##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24032968
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.901464022254764	37.0	35.0	38.0	33.0	39.0
2	35.27727049775958	35.0	34.0	37.0	33.0	39.0
3	35.40735709380547	35.0	34.0	37.0	33.0	39.0
4	35.47016381830159	36.0	35.0	38.0	33.0	39.0
5	35.38523215276615	36.0	35.0	37.0	33.0	39.0
6	35.3399336694494	35.0	34.0	37.0	33.0	39.0
7	35.36789155629883	35.0	34.0	37.0	33.0	39.0
8	35.518193633012785	36.0	34.0	38.0	33.0	39.0
9	35.4909180588931	36.0	34.0	38.0	33.0	39.0
10-14	35.45405915740411	35.8	34.4	37.8	33.0	39.0
15-19	35.42499393333358	36.0	34.0	38.0	33.0	39.0
20-24	35.395258088805335	36.0	34.0	38.0	33.0	39.0
25-29	35.38420476405578	36.0	34.0	38.0	33.0	39.0
30-34	35.36755524328081	36.0	34.0	38.0	33.0	39.0
35-39	35.34222502189492	36.0	34.0	38.0	32.8	39.0
40-44	35.32249301043466	36.0	34.0	38.0	32.2	39.0
45-49	35.293880747479875	36.0	34.0	38.0	32.0	39.0
50-54	35.2712216069193	36.0	34.0	38.0	32.0	39.0
55-59	35.242353728428384	36.0	34.0	38.0	32.0	39.0
60-64	35.202262999726045	35.8	34.0	38.0	32.0	39.0
65-69	35.168097887274904	35.6	34.0	37.8	32.0	39.0
70-74	35.14851536504716	35.6	34.0	37.6	32.0	39.0
75-79	35.098190222467345	35.0	34.0	37.0	32.0	39.0
80-84	35.02565785805294	35.0	34.0	37.0	32.0	39.0
85-89	35.008345222036766	35.0	34.0	37.0	32.0	39.0
90-94	34.960284504062756	35.0	34.0	37.0	31.6	39.0
95-99	34.89648774610422	35.0	34.0	37.0	31.0	39.0
100-104	34.83378322170967	35.0	34.0	37.0	31.0	39.0
105-109	34.77754403636159	35.0	34.0	37.0	31.0	39.0
110-114	34.71573126743884	35.0	34.0	37.0	31.0	39.0
115-119	34.65900770363786	35.0	34.0	37.0	30.4	39.0
120-124	34.58679424030121	35.0	34.0	37.0	30.0	39.0
125-129	34.50957267770585	35.0	34.0	37.0	30.0	39.0
130-134	34.436630485634026	35.0	34.0	37.0	30.0	39.0
135-139	34.35189166569318	35.0	34.0	37.0	29.8	39.0
140-144	34.25820801876468	35.0	34.0	37.0	29.0	39.0
145-149	34.22716553212235	35.0	34.0	37.0	29.2	39.0
150	35.023162751443216	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	150.0
22	1415.0
23	7631.0
24	27052.0
25	69007.0
26	144594.0
27	259202.0
28	389373.0
29	513797.0
30	657636.0
31	852667.0
32	1136859.0
33	1579264.0
34	2370325.0
35	4064725.0
36	1.0379069E7
37	1580182.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.187344983773954	18.572645709011056	16.81330412456755	31.426705182647435
2	23.722683773389953	25.355977672004553	33.49059092493278	17.43074762967271
3	19.86915640215557	29.257580670019617	30.167580633403247	20.705682294421564
4	23.35167674670894	34.05030539715278	22.356506279207792	20.241511576930492
5	25.004797576395895	35.1158666711494	22.631640836038226	17.247694916416485
6	18.860933031658845	38.65936575124637	23.13138768378504	19.348313533309742
7	20.185654971953525	20.362724237805335	38.28400220896562	21.167618581275523
8	21.547151396365194	23.009471822206894	28.543732093347774	26.899644688080144
9	23.407379396502336	23.02808375561437	28.85000720676697	24.71452964111632
10-14	23.112434552403183	28.827893417076076	25.82424276518822	22.23542926533252
15-19	22.902519572280877	28.079246807968122	27.237124436732074	21.781109183018927
20-24	22.99420257698744	28.24539900404665	27.036475765632694	21.723922653333215
25-29	23.1160048406506	28.2248545267056	26.96665286590198	21.692487766741817
30-34	23.107820250148215	28.0127334086471	27.11201568705135	21.767430654153333
35-39	23.18759297644802	28.007254867563592	27.08317674288086	21.72197541310753
40-44	23.34901956345966	27.79535677823896	27.154028582736846	21.701595075564533
45-49	23.35601911507559	27.621909204056692	27.241207993952308	21.78086368691541
50-54	23.431426558978703	27.609658762160866	27.204515290866404	21.75439938799402
55-59	23.60707840995752	27.485916845559817	27.10073179475793	21.80627294972473
60-64	23.50724055389247	27.468799525718175	27.175132925737678	21.84882699465168
65-69	23.59919556828063	27.389754784238907	27.116387774329542	21.89466187315092
70-74	23.670180226409506	27.364427745369767	27.0348510380857	21.930540990135025
75-79	23.581111836962997	27.41215121657243	27.13037497727997	21.87636196918461
80-84	23.684358041878664	27.43942730507214	27.022688389671135	21.85352626337806
85-89	23.705739540224116	27.3622403533451	27.077685665814872	21.854334440615915
90-94	23.63585066365927	27.294548585642957	27.14735271589545	21.92224803480232
95-99	23.720974897427435	27.41696848456635	27.13172792267403	21.730328695332183
100-104	23.864479128944108	27.393764463221697	26.947315494726716	21.79444091310748
105-109	23.648211613690798	27.53146308958196	27.050560031572008	21.769765265155236
110-114	23.67913350191972	27.50508658333545	27.010247504097194	21.805532410647633
115-119	23.757754014981185	27.518287166407813	27.011698165308033	21.71226065330297
120-124	23.721576408909513	27.417839990307396	27.209535092068197	21.65104850871489
125-129	23.701302222468257	27.493192762865874	27.171002030017107	21.634502984648762
130-134	23.749150184149208	27.40627766294854	27.239228194699344	21.60534395820291
135-139	23.66843657426174	27.42021401419914	27.333645215870995	21.577704195668126
140-144	23.69470615279647	27.523329629727044	27.31416496232094	21.467799255155544
145-149	23.388874236970196	27.52778641435536	27.373084361721546	21.710254986952897
150	23.518147281367586	26.360346879850287	27.819638314789703	22.30186752399242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	10.5
3	29.0
4	37.0
5	32.5
6	24.5
7	19.0
8	15.5
9	12.0
10	12.0
11	12.5
12	14.0
13	18.0
14	21.5
15	38.0
16	81.0
17	132.5
18	206.0
19	381.5
20	597.5
21	889.5
22	1404.0
23	2451.0
24	4119.0
25	6686.5
26	10616.5
27	16889.5
28	28769.0
29	44764.0
30	60017.5
31	76906.5
32	100414.5
33	132674.5
34	180325.0
35	248529.0
36	343846.0
37	464627.0
38	607341.5
39	787593.5
40	994842.5
41	1194969.0
42	1382288.0
43	1553219.0
44	1674446.5
45	1733483.5
46	1736485.0
47	1681333.5
48	1579523.0
49	1437987.0
50	1255592.5
51	1059491.5
52	870292.0
53	693914.0
54	543137.0
55	414390.0
56	310399.5
57	229461.0
58	165852.5
59	120020.0
60	84064.0
61	56526.5
62	38304.5
63	27174.0
64	19448.0
65	14064.5
66	10702.5
67	7910.0
68	6839.0
69	5243.0
70	3038.0
71	2335.0
72	1867.5
73	1488.5
74	1273.5
75	757.5
76	256.0
77	95.0
78	27.0
79	3.0
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.030059541543100293
25-29	8.321901814207883E-7
30-34	8.321901814207883E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.321901814207883E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.321908531856137E-7
75-79	6.607604282330082E-4
80-84	0.05811464210611996
85-89	8.321991429913361E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	3.3298251959980893E-6
140-144	3.1981626022822786E-4
145-149	0.031649342077004254
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	3.0
68-69	8.0
70-71	6.0
72-73	13.0
74-75	10.0
76-77	19.0
78-79	30.0
80-81	33.0
82-83	52.0
84-85	43.0
86-87	72.0
88-89	108.0
90-91	105.0
92-93	99.0
94-95	103.0
96-97	118.0
98-99	163.0
100-101	175.0
102-103	145.0
104-105	160.0
106-107	180.0
108-109	182.0
110-111	181.0
112-113	179.0
114-115	192.0
116-117	188.0
118-119	248.0
120-121	278.0
122-123	292.0
124-125	317.0
126-127	395.0
128-129	437.0
130-131	537.0
132-133	720.0
134-135	1056.0
136-137	1458.0
138-139	3268.0
140-141	6698.0
142-143	10698.0
144-145	71065.0
146-147	829500.0
148-149	1078978.0
150-151	2.2024456E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.68311036206983
#Duplication Level	Percentage of total
1	29.437300399632342
2	10.401922128589476
3	6.950070962795585
4	5.114998102141958
5	3.934844300510902
6	3.149704584959408
7	2.5386080011461813
8	2.17294882249545
9	1.8326433803669593
>10	21.460618531742153
>50	5.316859064531909
>100	6.026259217057779
>500	0.8560362644882759
>1k	0.7341701045936875
>5k	0.07301613494777
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.001423045210229548	0.0
2	2.912665634972759E-5	1.6643803628415766E-5	0.0	0.0	0.005675537037289776	0.0
3	7.0736165420767E-5	1.6643803628415766E-5	0.0	0.0	0.007106904149333532	0.0
4	1.206675763060143E-4	2.0804754535519707E-5	0.0	4.1609509071039415E-6	0.008588202672262535	0.0
5	1.206675763060143E-4	2.0804754535519707E-5	4.1609509071039415E-6	8.321901814207883E-6	0.010173524967869137	0.0
6	1.2482852721311825E-4	2.0804754535519707E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.011987699563366455	0.0
7	1.4563328174863795E-4	4.160950907103941E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.014367763482229911	0.0
8	1.9140374172678132E-4	4.5770459978143354E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.020134841439475974	0.0
9	1.9556469263388526E-4	4.5770459978143354E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.026588476296394187	0.0
10-11	2.3717420170492467E-4	4.785093543169533E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.03326680250229601	0.0
12-13	2.683813335082042E-4	5.201188633879927E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.039485343632962855	0.0
14-15	3.3079559711476333E-4	5.825331269945518E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.04799240776253687	0.0
16-17	3.599222534644909E-4	5.825331269945518E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.054150615105050696	0.0
18-19	4.3273889433880993E-4	6.241426360655912E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.062129238469422504	0.0
20-21	4.826703052240572E-4	7.281664087431897E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.06829576771375054	0.0
22-23	5.138774370273368E-4	9.570187086339066E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.07740616972485462	0.0
24-25	5.617283724590321E-4	1.0194329722404657E-4	4.1609509071039415E-6	1.6643803628415766E-5	0.08336673189928102	0.0
26-27	5.950159797158636E-4	1.0818472358470248E-4	4.1609509071039415E-6	2.0804754535519707E-5	0.08816014734426476	0.0
28-29	7.073616542076701E-4	1.1650662539891036E-4	4.1609509071039415E-6	2.0804754535519707E-5	0.09303054038102992	0.0
30-31	8.966849204808994E-4	1.289894781202222E-4	8.321901814207883E-6	2.0804754535519707E-5	0.09984201701595907	0.0
32-33	0.0010277548740546736	1.4355280629508598E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.10784976703668062	0.0
34-35	0.001144261499453584	1.4563328174863795E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.11187132608839656	0.0
36-37	0.0012170781403279029	1.6435756083060568E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.1156036990520688	0.0
38-39	0.0012670095512131501	1.7267946264481356E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.1199789389308886	0.0
40-41	0.0012982166830164297	1.8724279081967738E-4	1.2482852721311825E-5	2.288522998907168E-5	0.12280422459681217	0.0
42-43	0.0013127800111912935	2.0180611899454118E-4	1.4563328174863796E-5	2.496570544262365E-5	0.12577722401993796	0.0
44-45	0.001346067618448125	2.2677182443716482E-4	1.6643803628415766E-5	2.496570544262365E-5	0.1281739317424298	0.0
46-47	0.0013876771275191644	2.475765789726845E-4	1.6643803628415766E-5	2.496570544262365E-5	0.13092015934111842	0.0
48-49	0.0014376085384044117	2.642203826011003E-4	1.6643803628415766E-5	2.496570544262365E-5	0.1334375346399163	0.0
50-51	0.0015000228020109708	2.787837107759641E-4	1.6643803628415766E-5	2.496570544262365E-5	0.135607470537971	0.0
52-53	0.0015624370656175301	2.82944661683068E-4	1.6643803628415766E-5	2.496570544262365E-5	0.13800209778500933	0.0
54-55	0.0016019660992350176	2.9334703895082787E-4	2.080475453551971E-5	2.496570544262365E-5	0.14000975659768697	4.1609509071039415E-6
56-57	0.0016643803628415767	2.9542751440437984E-4	2.496570544262365E-5	2.496570544262365E-5	0.14208191014942473	4.1609509071039415E-6
58-59	0.0017226336755410319	3.0166894076503575E-4	2.704618089617562E-5	2.496570544262365E-5	0.143933533303086	4.1609509071039415E-6
60-61	0.0017954503164153508	3.183127443934515E-4	2.912665634972759E-5	2.496570544262365E-5	0.14547308513871446	4.1609509071039415E-6
62-63	0.0018162550709508705	3.2871512166121136E-4	2.912665634972759E-5	2.496570544262365E-5	0.1475847677240697	6.241426360655913E-6
64-65	0.00184746220275415	3.5160035165028304E-4	2.912665634972759E-5	2.704618089617562E-5	0.1493261256786927	8.321901814207883E-6
66-67	0.0019306812208962289	3.848879589071146E-4	2.912665634972759E-5	3.328760725683153E-5	0.15071796375711896	8.321901814207883E-6
68-69	0.0019556469263388525	3.9737081162842644E-4	2.912665634972759E-5	3.328760725683153E-5	0.1520328242437638	8.321901814207883E-6
70-71	0.001980612631781476	4.0985366434973826E-4	2.912665634972759E-5	3.328760725683153E-5	0.1533684894849442	8.321901814207883E-6
72-73	0.0019993369108634437	4.202560416174981E-4	2.912665634972759E-5	3.328760725683153E-5	0.1542901401108677	8.321901814207883E-6
74-75	0.002011819763584756	4.2441699252460205E-4	2.912665634972759E-5	3.328760725683153E-5	0.15537406782216828	8.321901814207883E-6
76-77	0.002049268321748691	4.368998452459139E-4	3.328760725683153E-5	3.328760725683153E-5	0.1568199982623869	8.321901814207883E-6
78-79	0.0021116825853552505	4.618655506885375E-4	3.744855816393547E-5	3.328760725683153E-5	0.15857175859427766	8.321901814207883E-6
80-81	0.002126245913530114	4.7226792795629737E-4	3.744855816393547E-5	3.328760725683153E-5	0.16017164421805913	1.0402377267759855E-5
82-83	0.00214288971715853	4.785093543169533E-4	3.952903361748744E-5	3.328760725683153E-5	0.16194212882903186	1.2482852721311825E-5
84-85	0.0021595335207869456	4.909922070382651E-4	4.160950907103941E-5	3.328760725683153E-5	0.16363563584822316	1.2482852721311825E-5
86-87	0.002205303980765089	4.930726824918171E-4	4.160950907103941E-5	3.328760725683153E-5	0.16553510993731613	1.4563328174863796E-5
88-89	0.0022219477843935047	4.99314108852473E-4	4.160950907103941E-5	3.328760725683153E-5	0.1673243188273708	1.6643803628415766E-5
90-91	0.0022365111125683687	5.180383879344407E-4	4.160950907103941E-5	3.744855816393547E-5	0.16819603804240907	1.6643803628415766E-5
92-93	0.0022510744407432323	5.242798142950966E-4	4.160950907103941E-5	3.744855816393547E-5	0.16915305675104297	1.6643803628415766E-5
94-95	0.00225939634255744	5.430040933770644E-4	4.577045997814336E-5	3.744855816393547E-5	0.17018497257600476	2.288522998907168E-5
96-97	0.0022614768180109924	5.492455197377203E-4	5.201188633879927E-5	3.952903361748744E-5	0.17110454272647474	2.496570544262365E-5
98-99	0.00228020109709296	5.554869460983762E-4	5.825331269945518E-5	4.160950907103941E-5	0.17200122764695563	2.496570544262365E-5
100-101	0.0022906034743607195	5.7005027427324E-4	5.825331269945518E-5	4.160950907103941E-5	0.17273563548205947	3.120713180327956E-5
102-103	0.0022968449007213757	5.762917006338959E-4	5.825331269945518E-5	4.160950907103941E-5	0.17358654994256223	3.328760725683153E-5
104-105	0.0022989253761749274	5.887745533552077E-4	5.825331269945518E-5	4.160950907103941E-5	0.17446034963305407	3.328760725683153E-5
106-107	0.0023280520325246554	6.074988324371754E-4	5.825331269945518E-5	4.3689984524591383E-5	0.17534871265172075	3.328760725683153E-5
108-109	0.002342615360699519	6.179012097049353E-4	5.825331269945518E-5	4.5770459978143354E-5	0.17627036327764428	3.328760725683153E-5
110-111	0.002369661541595695	6.345450133333511E-4	5.825331269945518E-5	4.5770459978143354E-5	0.17722530151082463	3.328760725683153E-5
112-113	0.0023800639188634545	6.428669151475589E-4	5.825331269945518E-5	4.785093543169533E-5	0.17793058268957873	3.328760725683153E-5
114-115	0.002390466296131214	6.532692924153188E-4	6.033378815300715E-5	4.99314108852473E-5	0.17876277287099954	3.328760725683153E-5
116-117	0.002400868673398974	6.574302433224227E-4	6.241426360655912E-5	5.201188633879927E-5	0.17964697493875914	3.744855816393547E-5
118-119	0.0024029491488525264	6.657521451366306E-4	6.657521451366306E-5	5.409236179235124E-5	0.18049372844835476	3.952903361748744E-5
120-121	0.00241751247702739	6.678326205901826E-4	6.657521451366306E-5	5.409236179235124E-5	0.18130511387524006	4.3689984524591383E-5
122-123	0.002425834378841598	6.719935714972865E-4	7.281664087431897E-5	5.617283724590321E-5	0.18203328028398325	4.5770459978143354E-5
124-125	0.00242791485429515	6.823959487650464E-4	7.489711632787095E-5	6.241426360655912E-5	0.1829528504344532	4.5770459978143354E-5
126-127	0.002436236756109358	6.990397523934621E-4	7.489711632787095E-5	6.657521451366306E-5	0.18401181244031117	5.409236179235124E-5
128-129	0.0024445586579235658	7.05281178754118E-4	7.489711632787095E-5	6.657521451366306E-5	0.18497923352621282	6.865568996721503E-5
130-131	0.0024508000842842215	7.198445069289818E-4	7.489711632787095E-5	6.657521451366306E-5	0.18605691981115274	7.489711632787095E-5
132-133	0.0024903291179017087	7.260859332896377E-4	7.489711632787095E-5	6.657521451366306E-5	0.1869369609280052	7.697759178142292E-5
134-135	0.0025090533969836766	7.385687860109497E-4	7.489711632787095E-5	6.657521451366306E-5	0.18782532394667192	7.905806723497489E-5
136-137	0.0025132143478907806	7.656149669071253E-4	7.697759178142292E-5	7.0736165420767E-5	0.18875113552350253	7.905806723497489E-5
138-139	0.0025173752987978845	7.760173441748851E-4	8.946044450273474E-5	7.0736165420767E-5	0.1895375552449452	8.321901814207883E-5
>>END_MODULE
