##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23616406
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04884117422439	37.0	35.0	38.0	33.0	39.0
2	35.35801925153218	35.0	34.0	37.0	33.0	39.0
3	35.43290283034599	35.0	34.0	37.0	33.0	39.0
4	35.54279724018972	36.0	35.0	38.0	33.0	39.0
5	35.47198100337537	36.0	35.0	38.0	33.0	39.0
6	35.405520425080766	35.0	35.0	37.0	33.0	39.0
7	35.453371524862845	35.0	34.0	38.0	33.0	39.0
8	35.56127431074822	36.0	35.0	38.0	33.0	39.0
9	35.57688604269422	36.0	35.0	38.0	33.0	39.0
10-14	35.534149607692214	35.8	35.0	37.8	33.0	39.0
15-19	35.50204664503142	36.0	35.0	38.0	33.0	39.0
20-24	35.478268403752885	36.0	34.8	38.0	33.0	39.0
25-29	35.46513819249211	36.0	34.6	38.0	33.0	39.0
30-34	35.45388971547999	36.0	34.0	38.0	33.0	39.0
35-39	35.42032244025615	36.0	34.0	38.0	33.0	39.0
40-44	35.41078736535949	36.0	34.0	38.0	33.0	39.0
45-49	35.37629798539202	36.0	34.0	38.0	32.6	39.0
50-54	35.3583651805444	36.0	34.0	38.0	32.0	39.0
55-59	35.32458337648836	36.0	34.0	38.0	32.0	39.0
60-64	35.28385742382367	36.0	34.0	38.0	32.0	39.0
65-69	35.24436056363486	36.0	34.0	38.0	32.0	39.0
70-74	35.22780945188002	36.0	34.0	38.0	32.0	39.0
75-79	35.16461945319644	36.0	34.0	38.0	32.0	39.0
80-84	35.093147029455196	36.0	34.0	38.0	32.0	39.0
85-89	35.07003530629779	36.0	34.0	38.0	32.0	39.0
90-94	35.02155211230376	35.6	34.0	38.0	31.6	39.0
95-99	34.953036111876074	35.2	34.0	37.6	31.0	39.0
100-104	34.89065393611958	35.0	34.0	37.4	31.0	39.0
105-109	34.832223242941964	35.0	34.0	37.2	31.0	39.0
110-114	34.75909242806815	35.0	34.0	37.0	30.8	39.0
115-119	34.696249522608774	35.0	34.0	37.0	30.0	39.0
120-124	34.62122216333821	35.0	34.0	37.0	30.0	39.0
125-129	34.539154683670915	35.0	34.0	37.0	30.0	39.0
130-134	34.45779963138857	35.0	34.0	37.0	30.0	39.0
135-139	34.37496160039006	35.0	34.0	37.0	29.6	39.0
140-144	34.281432865684295	35.0	34.0	37.0	29.0	39.0
145-149	34.24550528357675	35.0	34.0	37.0	29.0	39.0
150	35.04917573487328	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	119.0
22	1158.0
23	6461.0
24	23669.0
25	61057.0
26	130452.0
27	239076.0
28	363226.0
29	485781.0
30	630613.0
31	825764.0
32	1105592.0
33	1541470.0
34	2309341.0
35	3917146.0
36	9859684.0
37	2115786.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.368392802867636	19.321462376620726	12.902479742260528	36.407665078251114
2	25.103125344305145	25.621074603815668	32.91319602144374	16.362604030435453
3	19.065978963945657	29.86556464180028	30.12388506532281	20.944571328931254
4	22.734564268585153	34.32960967896639	21.645380757766443	21.290445294682012
5	25.717668471654832	35.25940399229248	21.353503153697474	17.669424382355214
6	18.943403157957228	38.77200874680084	22.04177045398017	20.242817641261755
7	20.24024739412085	20.519396558477187	36.9953582268191	22.24499782058286
8	20.770844640797588	23.130136736301026	28.876654644233334	27.222363978668053
9	23.26085518685612	23.24464188158012	28.04548244978512	25.44902048177864
10-14	23.126573958797962	28.692160017912972	25.156323955474	23.02494206781506
15-19	23.364766397812883	27.58012167609452	26.526116235063306	22.52899569102929
20-24	23.26603060990281	27.56436917671466	26.424456973379435	22.7451432400031
25-29	23.352760788411242	27.194609543890802	26.432839103460537	23.01979056423742
30-34	23.154182732122745	26.907742863160465	26.639786765183494	23.298287639533296
35-39	23.505904327694907	26.69510085488876	26.662157654301843	23.13683716311449
40-44	23.71294768560466	26.36609905842574	26.628327781966487	23.292625474003113
45-49	23.642664341051724	26.218434760987762	26.703777873737433	23.435123024223074
50-54	23.781267342552184	26.17263885260644	26.50701826947774	23.53907553536364
55-59	23.834932377094127	26.236099599574974	26.500958697949212	23.428009325381684
60-64	23.505691513916847	26.18706316753861	26.610417180592606	23.696828137951933
65-69	23.699267678517018	26.13487641895015	26.615698139310282	23.550157763222547
70-74	23.893619688013416	26.118114530580446	26.31915533273488	23.669110448671262
75-79	23.715204282065226	26.183143754974324	26.66202212813642	23.43962983482403
80-84	24.06123882534898	26.22956979780604	26.30333358960588	23.405857787239096
85-89	23.753938943495395	26.318448788320925	26.56848293211925	23.35912933606443
90-94	23.73499882402833	26.03353140176331	26.633090640197075	23.598379134011278
95-99	23.743760485854278	26.25227442280313	26.67257495199801	23.33139013934459
100-104	24.027707613247077	26.01694954871292	26.566441567673277	23.388901270366727
105-109	23.8311561107068	26.192057684882545	26.527368536114366	23.44941766829629
110-114	23.891336739905885	26.350052697374977	26.41021765459413	23.348392908125007
115-119	24.001902515717944	26.380127620320735	26.570855159811657	23.04711470414966
120-124	23.96458204550006	26.580615435556492	26.6376403288691	22.81716219007435
125-129	23.958898588592678	26.62899341909694	26.532456466815475	22.879651525494907
130-134	24.14398215785255	26.611480659860504	26.542953833656917	22.70158334863003
135-139	23.87687769556401	26.667303159280305	26.83214746136183	22.62367168379386
140-144	23.916046196529244	26.84460608333998	26.623181545473436	22.616166174657344
145-149	23.719493774121858	27.099548280756103	26.4677506617852	22.713207283336835
150	23.918373176789682	25.984735868577445	26.716165232986256	23.380725721646616
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	7.5
3	15.5
4	30.0
5	30.0
6	17.5
7	19.0
8	14.5
9	5.0
10	6.5
11	6.5
12	6.0
13	9.0
14	19.5
15	31.5
16	53.0
17	94.0
18	179.0
19	329.0
20	500.5
21	724.0
22	1120.5
23	1827.0
24	2898.5
25	4655.0
26	7213.5
27	10742.5
28	16274.0
29	23362.5
30	32719.5
31	46293.5
32	65474.5
33	89400.5
34	120600.5
35	169778.5
36	239694.0
37	325829.0
38	434915.0
39	567455.5
40	725127.5
41	904244.5
42	1095318.0
43	1281186.0
44	1430139.0
45	1538864.5
46	1584484.5
47	1561810.5
48	1531939.0
49	1500589.0
50	1399126.0
51	1244614.0
52	1071495.5
53	900389.5
54	759571.0
55	646873.5
56	541319.0
57	443016.0
58	370372.0
59	305450.5
60	231616.5
61	155804.0
62	94219.5
63	56609.5
64	35547.5
65	18364.5
66	10237.5
67	5694.5
68	3175.5
69	1931.5
70	1192.5
71	1069.5
72	889.5
73	709.5
74	581.0
75	359.0
76	122.0
77	36.0
78	10.0
79	2.0
80	1.0
81	0.0
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.6937378193786132E-6
20-24	0.024774303084050976
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.468689096893066E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.468691176735733E-7
75-79	4.954187527007203E-4
80-84	0.04774657587692244
85-89	1.693745780191177E-6
90-94	0.0
95-99	0.0
100-104	8.468863018300238E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	2.6105718735698006E-4
145-149	0.026500317408810415
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	1.0
66-67	0.0
68-69	2.0
70-71	1.0
72-73	3.0
74-75	10.0
76-77	8.0
78-79	8.0
80-81	20.0
82-83	20.0
84-85	20.0
86-87	32.0
88-89	33.0
90-91	29.0
92-93	34.0
94-95	45.0
96-97	69.0
98-99	58.0
100-101	93.0
102-103	84.0
104-105	81.0
106-107	104.0
108-109	121.0
110-111	118.0
112-113	126.0
114-115	149.0
116-117	171.0
118-119	174.0
120-121	173.0
122-123	236.0
124-125	265.0
126-127	307.0
128-129	375.0
130-131	463.0
132-133	596.0
134-135	866.0
136-137	1514.0
138-139	4395.0
140-141	8880.0
142-143	10060.0
144-145	59658.0
146-147	780875.0
148-149	1070214.0
150-151	2.1675914E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.267536482703676
#Duplication Level	Percentage of total
1	24.161990557164863
2	7.9700128845678115
3	4.991677479337297
4	3.6554829141008707
5	2.653494149809223
6	2.199294355500777
7	1.759955871212475
8	1.4733769240870684
9	1.3209236844570813
>10	17.003514154284975
>50	6.146967688919714
>100	13.413849291549099
>500	4.879075830054635
>1k	6.630331493360729
>5k	1.052965413500191
>10k+	0.6870873080931945
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	29717	0.12583201694618562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.81091009360188E-4	4.234344548446533E-6	0.0	0.0	0.002371232947130059	0.0
2	0.0013719276336966767	1.6937378193786134E-5	0.0	4.234344548446533E-6	0.010835687699474678	0.0
3	0.002206093509740644	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.015946541569449645	0.0
4	0.005762942930435732	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.025080022760448815	0.0
5	0.005974660157858059	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.03144424261676396	0.0
6	0.007100995807744836	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.041107016876318944	0.0
7	0.009184293325580531	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.054885574036963966	0.0
8	0.016251414376937796	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.07240729177843572	0.0
9	0.0169162064710439	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.08395011501750096	0.0
10-11	0.017650865250199377	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.140372756125551	0.0
12-13	0.018279665415643687	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.16638010034210965	0.0
14-15	0.020801217594243596	2.54060672906792E-5	0.0	4.234344548446533E-6	0.17790386903070687	0.0
16-17	0.023718680988123256	2.54060672906792E-5	0.0	4.234344548446533E-6	0.1861057944210478	0.0
18-19	0.029468920884913652	3.1757584113349E-5	0.0	4.234344548446533E-6	0.1936048186163466	0.0
20-21	0.034626352544921524	3.387475638757227E-5	0.0	4.234344548446533E-6	0.19768037524422638	0.0
22-23	0.03670329854593456	5.504647912980493E-5	0.0	4.234344548446533E-6	0.20239955224347006	0.0
24-25	0.04151139678069559	5.928082367825146E-5	0.0	4.234344548446533E-6	0.20730927474739383	0.0
26-27	0.04561871099268873	6.774951277514453E-5	0.0	4.234344548446533E-6	0.21130861317340158	0.0
28-29	0.05662165530182704	7.198385732359107E-5	0.0	4.234344548446533E-6	0.21482311914861218	0.0
30-31	0.08006933823884972	7.198385732359107E-5	0.0	4.234344548446533E-6	0.21840960898114642	0.0
32-33	0.10136173980071311	7.198385732359107E-5	0.0	4.234344548446533E-6	0.2212931976186385	0.0
34-35	0.1167938085075265	7.198385732359107E-5	0.0	4.234344548446533E-6	0.22495378848077052	0.0
36-37	0.11856588170105138	7.198385732359107E-5	0.0	4.234344548446533E-6	0.22691005566215283	0.0
38-39	0.12037182965096382	7.833537414626086E-5	0.0	4.234344548446533E-6	0.22863343389337057	0.0
40-41	0.12174587445693472	8.680406324315394E-5	0.0	4.234344548446533E-6	0.23039492122552432	0.0
42-43	0.12201475533576109	8.89212355173772E-5	0.0	4.234344548446533E-6	0.23180495796015702	0.0
44-45	0.12271553935852898	9.103840779160047E-5	0.0	4.234344548446533E-6	0.23307314415241676	0.0
46-47	0.1234078546922	1.0374144143694007E-4	0.0	4.234344548446533E-6	0.2344344859247423	0.0
48-49	0.12418697408911415	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.23552694681824152	0.0
50-51	0.12494280459101186	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.23664693095130562	0.0
52-53	0.12576003308886202	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.23783043025259643	0.0
54-55	0.12617711602688403	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.2387471658473351	4.234344548446533E-6
56-57	0.12690542328921683	1.143273028080564E-4	0.0	4.234344548446533E-6	0.2396829559925418	4.234344548446533E-6
58-59	0.12703880514249288	1.2279599190494946E-4	0.0	4.234344548446533E-6	0.2405488794526991	4.234344548446533E-6
60-61	0.12725052236991524	1.27030336453396E-4	0.0	4.234344548446533E-6	0.24124542913091856	4.234344548446533E-6
62-63	0.12750881738737047	1.3338185327606581E-4	0.0	4.234344548446533E-6	0.24219180513749639	4.234344548446533E-6
64-65	0.12754269214375802	1.460848869214054E-4	0.0	4.234344548446533E-6	0.24308525183721857	4.234344548446533E-6
66-67	0.12760197296743628	1.7572529876053113E-4	0.0	4.234344548446533E-6	0.24377544999861536	4.234344548446533E-6
68-69	0.12765278510201766	1.7995964330897766E-4	0.0	4.234344548446533E-6	0.244615967391482	4.234344548446533E-6
70-71	0.1277501750266319	1.8631116013164746E-4	0.0	4.234344548446533E-6	0.24551364843575268	4.234344548446533E-6
72-73	0.12780945585031017	1.884283324058707E-4	0.0	4.234344548446533E-6	0.24600059805882402	8.468689096893067E-6
74-75	0.1278306275730524	2.0113136605121035E-4	0.0	4.234344548446533E-6	0.24665056994701057	1.4820205919562867E-5
76-77	0.12786026798489153	2.0960005514810342E-4	0.0	4.234344548446533E-6	0.2473555883143269	1.6937378193786134E-5
78-79	0.12792801749766666	2.3288895016455933E-4	0.0	4.234344548446533E-6	0.24862165733431243	1.6937378193786134E-5
80-81	0.12801905590545826	2.3712329471300587E-4	0.0	4.234344548446533E-6	0.24955109596269642	1.6937378193786134E-5
82-83	0.12804234480047472	2.455919838098989E-4	2.1171722742232667E-6	4.234344548446533E-6	0.25051652651974227	1.6937378193786134E-5
84-85	0.12808045390141076	2.54060672906792E-4	4.234344548446533E-6	6.3515168226698E-6	0.25136762977398	1.6937378193786134E-5
86-87	0.12812703169144365	2.5617784518101525E-4	4.234344548446533E-6	8.468689096893067E-6	0.2521954441332013	1.9054550468009402E-5
88-89	0.12816514079237967	2.5829501745523855E-4	4.234344548446533E-6	8.468689096893067E-6	0.25306560193790706	2.1171722742232667E-5
90-91	0.1282032498933157	2.6252936200368506E-4	4.234344548446533E-6	1.27030336453396E-5	0.253700753620174	2.752323956490247E-5
92-93	0.12823500747742905	2.6252936200368506E-4	4.234344548446533E-6	1.27030336453396E-5	0.25424910123919786	2.9640411839125734E-5
94-95	0.12831546002384953	2.6676370655213163E-4	4.234344548446533E-6	1.27030336453396E-5	0.2551086731825325	2.9640411839125734E-5
96-97	0.12833028022976908	2.837010847459178E-4	4.234344548446533E-6	1.27030336453396E-5	0.2557988713439293	2.9640411839125734E-5
98-99	0.12834933478023708	3.0063846293970387E-4	4.234344548446533E-6	1.4820205919562867E-5	0.25639167958071185	2.9640411839125734E-5
100-101	0.1283789751920762	3.048728074881504E-4	4.234344548446533E-6	1.6937378193786134E-5	0.2569971908511397	2.9640411839125734E-5
102-103	0.12840014691481844	3.048728074881504E-4	4.234344548446533E-6	1.6937378193786134E-5	0.2576492799116004	2.9640411839125734E-5
104-105	0.1284234358098349	3.0910715203659694E-4	4.234344548446533E-6	1.6937378193786134E-5	0.2583225406948034	2.9640411839125734E-5
106-107	0.12844249036030292	3.1334149658504345E-4	4.234344548446533E-6	1.6937378193786134E-5	0.25899791865028066	3.387475638757227E-5
108-109	0.1284678964275936	3.2392735795615983E-4	8.468689096893067E-6	2.1171722742232667E-5	0.25962883598799924	3.5991928661795536E-5
110-111	0.1284975368394327	3.366303916014994E-4	8.468689096893067E-6	2.1171722742232667E-5	0.2602195270525075	3.81091009360188E-5
112-113	0.12852717725127186	3.5145059752106226E-4	8.468689096893067E-6	2.1171722742232667E-5	0.2608356241843065	3.81091009360188E-5
114-115	0.1285525833185625	3.535677697952855E-4	1.0585861371116334E-5	2.1171722742232667E-5	0.26148136172794456	3.81091009360188E-5
116-117	0.1285674035244821	3.5991928661795533E-4	1.27030336453396E-5	2.1171722742232667E-5	0.26212498209930846	3.81091009360188E-5
118-119	0.12859280959177277	3.5991928661795533E-4	1.27030336453396E-5	2.1171722742232667E-5	0.26269238426880026	3.81091009360188E-5
120-121	0.12860127828086967	3.5991928661795533E-4	1.27030336453396E-5	2.54060672906792E-5	0.2633127157451477	3.81091009360188E-5
122-123	0.12860974696996658	3.6415363116640184E-4	1.27030336453396E-5	2.54060672906792E-5	0.26401349976791555	3.81091009360188E-5
124-125	0.128633035864983	3.6627080344062515E-4	1.27030336453396E-5	2.54060672906792E-5	0.2647164009629577	4.0226273210242066E-5
126-127	0.12864785607090257	3.683879757148484E-4	1.27030336453396E-5	2.54060672906792E-5	0.2654510597421132	4.4460617758688596E-5
128-129	0.12866267627682215	3.683879757148484E-4	1.27030336453396E-5	2.54060672906792E-5	0.26619418721036553	4.6577790032911864E-5
130-131	0.12869231668866127	3.768566648117415E-4	1.27030336453396E-5	2.54060672906792E-5	0.267185023834702	5.08121345813584E-5
132-133	0.12871983992822617	3.768566648117415E-4	1.27030336453396E-5	2.54060672906792E-5	0.26789427654656683	5.504647912980493E-5
134-135	0.1287515975123395	3.768566648117415E-4	1.27030336453396E-5	3.1757584113349E-5	0.26848920195562354	5.928082367825147E-5
136-137	0.1287791207519044	3.768566648117415E-4	1.27030336453396E-5	3.387475638757227E-5	0.26912223646561634	6.3515168226698E-5
138-139	0.12878335509645286	3.7897383708596473E-4	1.27030336453396E-5	3.387475638757227E-5	0.2698314891774811	6.98666850493678E-5
>>END_MODULE
