##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134792
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	53-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.95306153871142	37.0	35.0	38.0	33.0	39.0
2	35.33436327108185	35.0	34.0	37.0	33.0	39.0
3	35.44602410495189	35.0	34.0	37.0	33.0	39.0
4	35.51584571352428	36.0	35.0	38.0	33.0	39.0
5	35.43642874568797	36.0	35.0	37.0	33.0	39.0
6	35.386232870786706	35.0	34.0	37.0	33.0	39.0
7	35.435502613819914	35.0	34.0	38.0	33.0	39.0
8	35.546135926922425	36.0	35.0	38.0	33.0	39.0
9	35.5392435948899	36.0	35.0	38.0	33.0	39.0
10-14	35.49999028787983	35.8	34.4	37.8	33.0	39.0
15-19	35.47174599225881	36.0	34.0	38.0	33.0	39.0
20-24	35.44528343977442	36.0	34.0	38.0	33.0	39.0
25-29	35.43808870613013	36.0	34.0	38.0	33.0	39.0
30-34	35.421217452381605	36.0	34.0	38.0	33.0	39.0
35-39	35.39402953213767	36.0	34.0	38.0	33.0	39.0
40-44	35.378495799756635	36.0	34.0	38.0	32.6	39.0
45-49	35.34966090447351	36.0	34.0	38.0	32.0	39.0
50-54	35.331034577084516	36.0	34.0	38.0	32.0	39.0
55-59	35.29983863543712	36.0	34.0	38.0	32.0	39.0
60-64	35.26046322920301	36.0	34.0	38.0	32.0	39.0
65-69	35.2220859246181	36.0	34.0	38.0	32.0	39.0
70-74	35.20400766829461	36.0	34.0	38.0	32.0	39.0
75-79	35.14768520081559	36.0	34.0	38.0	32.0	39.0
80-84	35.07464275125809	35.8	34.0	38.0	32.0	39.0
85-89	35.056457459806204	35.8	34.0	38.0	32.0	39.0
90-94	35.00905336074801	35.2	34.0	38.0	31.6	39.0
95-99	34.94423338400692	35.2	34.0	37.2	31.0	39.0
100-104	34.87988870264614	35.0	34.0	37.0	31.0	39.0
105-109	34.82485407636536	35.0	34.0	37.0	31.0	39.0
110-114	34.75352596699521	35.0	34.0	37.0	31.0	39.0
115-119	34.69089069987767	35.0	34.0	37.0	30.4	39.0
120-124	34.617592956392976	35.0	34.0	37.0	30.0	39.0
125-129	34.538704844974305	35.0	34.0	37.0	30.0	39.0
130-134	34.46164823567408	35.0	34.0	37.0	30.0	39.0
135-139	34.37719692819377	35.0	34.0	37.0	30.0	39.0
140-144	34.28439154157854	35.0	34.0	37.0	29.0	39.0
145-149	34.24625576904233	35.0	34.0	37.0	29.0	39.0
150	35.04308733697294	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	112.0
22	1297.0
23	7354.0
24	25814.0
25	67503.0
26	140574.0
27	250485.0
28	376875.0
29	501850.0
30	648445.0
31	848263.0
32	1135763.0
33	1584267.0
34	2364899.0
35	4011094.0
36	1.0010003E7
37	2160180.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.361940720268066	19.21183741711965	15.120934126964922	32.30528773564736
2	24.4421590392061	26.00567786147392	31.894869112394638	17.65729398692535
3	20.155976484073282	29.496363589957603	29.21511401465569	21.13254591131343
4	23.75049264978128	34.02738668723559	21.333401174536743	20.888719488446387
5	25.664410117974086	34.96309808677862	21.31907331125953	18.053418483987762
6	19.488964313427687	38.78851742331154	21.676698104545505	20.04582015871527
7	20.814250232610252	20.793690701788524	36.209083550419656	22.182975515181568
8	21.92304371216458	23.58336877318023	27.594723004035004	26.898864510620186
9	23.75044707242557	23.576726909434313	27.217736121363718	25.455089896776407
10-14	23.37680722502187	28.802471552271925	24.794623463090133	23.026097759616075
15-19	23.471256519747673	27.67196405103381	26.309739722879893	22.547039706338627
20-24	23.327086212489135	27.645092128067393	26.27336258684384	22.754459072599637
25-29	23.43854319058182	27.24195946865388	26.250827653711106	23.068669687053195
30-34	23.24194631550999	26.982675467018737	26.494606624328892	23.280771593142383
35-39	23.56297663555584	26.701372027569164	26.55648244244243	23.17916889443257
40-44	23.73580845445032	26.398716011308487	26.570531869510205	23.294943664730983
45-49	23.638465995480715	26.222328329989335	26.665782742192267	23.47342293233768
50-54	23.83193480734261	26.229622798980373	26.37452315933816	23.56391923433886
55-59	23.86210180978986	26.229785872187584	26.44292216990816	23.465190148114395
60-64	23.528976074414732	26.20164724028478	26.504837767188455	23.76453891811203
65-69	23.76043111408526	26.1673092977622	26.53135721063778	23.540902377514765
70-74	23.93556235439119	26.123699409444118	26.256771232553948	23.683967003610746
75-79	23.71825006689347	26.230918019772194	26.55731193089117	23.49351998244316
80-84	24.04569660627233	26.226710870420096	26.241481089294272	23.486111434013303
85-89	23.726878741809102	26.325951380575603	26.50777079788302	23.439399079732283
90-94	23.746235763388935	26.049292168470274	26.56420349020975	23.640268577931046
95-99	23.722969087264502	26.258869793229913	26.6051090443237	23.413052075181888
100-104	23.993533795227833	26.040111206841914	26.55098487373743	23.41537012419282
105-109	23.784526815540143	26.229487356914312	26.486856378557512	23.499129448988032
110-114	23.87531568358505	26.322766295435272	26.382208830711573	23.419709190268097
115-119	23.985648207041063	26.411392926947812	26.536959927602922	23.0659989384082
120-124	23.976688497170624	26.550192413056774	26.632470343116065	22.840648746656537
125-129	23.917459747430613	26.642490548125863	26.488476090771506	22.951573613672018
130-134	24.08255761856795	26.654038991526026	26.50526479769317	22.758138592212855
135-139	23.843039698782114	26.675465765287882	26.781342733575126	22.70015180235488
140-144	23.890086789240648	26.78391876612268	26.597740048819173	22.728254395817498
145-149	23.6452668768041	26.943789742716994	26.529326886095784	22.88161649438312
150	23.799372461331085	25.86576442955743	26.799471641293128	23.535391467818357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	10.0
3	28.0
4	31.5
5	29.5
6	28.5
7	21.0
8	13.5
9	10.5
10	6.5
11	2.0
12	4.0
13	8.0
14	15.0
15	25.0
16	34.5
17	68.0
18	128.5
19	213.0
20	368.5
21	564.0
22	909.0
23	1521.0
24	2414.0
25	3972.5
26	6274.5
27	9650.0
28	14663.5
29	20969.5
30	29483.0
31	42283.0
32	61104.0
33	84635.0
34	115367.5
35	164636.0
36	234783.0
37	320249.5
38	429272.0
39	565968.5
40	728575.0
41	910964.0
42	1106429.5
43	1297521.0
44	1453533.0
45	1566192.5
46	1615806.0
47	1600357.5
48	1572459.0
49	1548140.0
50	1455596.5
51	1299115.5
52	1115444.0
53	938541.5
54	793506.5
55	672634.0
56	562215.0
57	458554.0
58	380358.0
59	313145.0
60	237031.0
61	158184.5
62	96753.0
63	58401.5
64	36360.0
65	19394.5
66	11278.5
67	6367.0
68	3719.5
69	2417.0
70	1415.0
71	1236.5
72	1147.0
73	932.5
74	722.0
75	413.5
76	138.0
77	43.5
78	14.5
79	3.5
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.143395973746117E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	2.4860375842476703E-6
20-24	0.028052448100650714
25-29	8.286791947492234E-7
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.286792016163155E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	2.486038119880968E-6
75-79	5.618448432318226E-4
80-84	0.054878532851129716
85-89	1.65736358103632E-6
90-94	0.0
95-99	8.286883418168368E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.287211479385673E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	8.288733591913087E-7
140-144	2.9110685236253907E-4
145-149	0.029418482993905863
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52-53	1.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.0
66-67	0.0
68-69	1.0
70-71	2.0
72-73	3.0
74-75	4.0
76-77	6.0
78-79	7.0
80-81	10.0
82-83	13.0
84-85	18.0
86-87	21.0
88-89	19.0
90-91	44.0
92-93	48.0
94-95	32.0
96-97	66.0
98-99	76.0
100-101	82.0
102-103	70.0
104-105	83.0
106-107	81.0
108-109	100.0
110-111	109.0
112-113	105.0
114-115	134.0
116-117	153.0
118-119	201.0
120-121	218.0
122-123	235.0
124-125	257.0
126-127	339.0
128-129	422.0
130-131	476.0
132-133	573.0
134-135	817.0
136-137	1451.0
138-139	4492.0
140-141	8853.0
142-143	9954.0
144-145	61240.0
146-147	788361.0
148-149	1073714.0
150-151	2.21819E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.918525140975134
#Duplication Level	Percentage of total
1	24.79214778883038
2	8.090997986383323
3	5.04213592643829
4	3.49451889833562
5	2.624293062794434
6	2.179545215329719
7	1.769806251388329
8	1.5258327738422621
9	1.270602106183109
>10	16.786745396742177
>50	6.137347488494746
>100	13.570587142182816
>500	4.752401212104629
>1k	6.33702999897808
>5k	1.0708402283190583
>10k+	0.555168523653023
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	26519	0.10987871782777328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.356150738734355E-4	0.0	0.0	0.0	0.002548188523853862	0.0
2	0.001798233852605815	1.2430187921238352E-5	0.0	0.0	0.010370920122286532	0.0
3	0.0026144828594338	1.2430187921238352E-5	0.0	0.0	0.014373440632925281	0.0
4	0.006227524148540414	1.657358389498447E-5	0.0	4.143395973746117E-6	0.020609251573413187	0.0
5	0.006488558094886419	1.657358389498447E-5	0.0	4.143395973746117E-6	0.024789938110923018	0.0
6	0.007665282551430317	1.657358389498447E-5	0.0	4.143395973746117E-6	0.032272911239508505	0.0
7	0.00986956920946325	2.4860375842476704E-5	0.0	4.143395973746117E-6	0.043385499241095596	0.0
8	0.01665645181445939	2.900377181622282E-5	0.0	4.143395973746117E-6	0.05861662284058632	0.0
9	0.01713708574741394	2.900377181622282E-5	0.0	4.143395973746117E-6	0.0699529542247557	0.0
10-11	0.017756523445488986	2.900377181622282E-5	0.0	4.143395973746117E-6	0.11682097778178491	0.0
12-13	0.018603847922120068	2.900377181622282E-5	0.0	4.143395973746117E-6	0.13977953487231215	0.0
14-15	0.021701036412495288	3.5218865776842E-5	0.0	4.143395973746117E-6	0.15035348139731225	0.0
16-17	0.025208421104271378	3.9362261750588117E-5	0.0	4.143395973746117E-6	0.15824043563333795	0.0
18-19	0.03217139803815173	4.972075168495341E-5	0.0	4.143395973746117E-6	0.16523655973500828	0.0
20-21	0.03795350711951444	4.972075168495341E-5	0.0	4.143395973746117E-6	0.1696782802188641	0.0
22-23	0.04035460508630031	4.972075168495341E-5	8.286791947492234E-6	4.143395973746117E-6	0.17434581578328912	0.0
24-25	0.04576380853002586	5.179244967182646E-5	8.286791947492234E-6	4.143395973746117E-6	0.1786383740120901	0.0
26-27	0.050298755423290986	5.386414765869952E-5	8.286791947492234E-6	4.143395973746117E-6	0.18200281154277193	0.0
28-29	0.06111509061275523	5.386414765869952E-5	8.286791947492234E-6	4.143395973746117E-6	0.18519529814054334	0.0
30-31	0.08348114207903677	5.386414765869952E-5	1.0358489934365292E-5	4.143395973746117E-6	0.18864053189271324	0.0
32-33	0.10253662016229517	5.800754363244564E-5	1.2430187921238352E-5	4.143395973746117E-6	0.19118457702059333	0.0
34-35	0.11625540423136856	5.800754363244564E-5	1.2430187921238352E-5	8.286791947492234E-6	0.19407252401429437	0.0
36-37	0.11887403048677611	5.800754363244564E-5	1.2430187921238352E-5	8.286791947492234E-6	0.19596812767228322	0.0
38-39	0.12115496997032334	5.800754363244564E-5	1.2430187921238352E-5	8.286791947492234E-6	0.19739967098121253	0.0
40-41	0.12257822648730513	6.215093960619175E-5	1.2430187921238352E-5	1.0358489934365292E-5	0.19873384448475878	0.0
42-43	0.12284340382962489	6.629433557993787E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20018196137758304	0.0
44-45	0.12363686415859726	8.079622148804929E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20138561790795628	0.0
46-47	0.12494410558831416	9.115471142241458E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.2027052895255944	2.0716979868730586E-6
48-49	0.1260255319374619	9.52981073961607E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20377428568682093	4.143395973746117E-6
50-51	0.12700751678323974	9.52981073961607E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.2048847158077849	4.143395973746117E-6
52-53	0.1280392223807025	9.52981073961607E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20601793460660445	6.215093960619176E-6
54-55	0.12854264499151266	1.0151320135677987E-4	1.2430187921238352E-5	1.2430187921238352E-5	0.2069356968147892	8.286791947492234E-6
56-57	0.1293485355084063	1.0772829531739904E-4	1.2430187921238352E-5	1.2430187921238352E-5	0.20784102883505273	1.0358489934365292E-5
58-59	0.1295080562533955	1.0772829531739904E-4	1.450188590811141E-5	1.450188590811141E-5	0.20867592312376257	1.2430187921238352E-5
60-61	0.1298022373675315	1.0772829531739904E-4	1.657358389498447E-5	1.657358389498447E-5	0.20933265138560134	1.2430187921238352E-5
62-63	0.13005498452192998	1.0979999330427211E-4	1.657358389498447E-5	1.657358389498447E-5	0.21016133058035055	1.2430187921238352E-5
64-65	0.13007984489777247	1.201584832386374E-4	1.657358389498447E-5	1.657358389498447E-5	0.21094650411737545	1.2430187921238352E-5
66-67	0.1301627128172474	1.3258867115987575E-4	1.657358389498447E-5	1.657358389498447E-5	0.21152243615772615	1.2430187921238352E-5
68-69	0.13023522224678796	1.40875463107368E-4	1.657358389498447E-5	1.657358389498447E-5	0.21221438328534176	1.2430187921238352E-5
70-71	0.13039059959580346	1.5123395304173328E-4	1.657358389498447E-5	1.657358389498447E-5	0.21290011531899672	1.2430187921238352E-5
72-73	0.13044653544144902	1.6159244297609857E-4	1.657358389498447E-5	1.657358389498447E-5	0.21339317943987252	1.2430187921238352E-5
74-75	0.1304776109112521	1.6159244297609857E-4	1.657358389498447E-5	1.657358389498447E-5	0.21395253789632826	1.2430187921238352E-5
76-77	0.13050247128709458	1.6366414096297163E-4	2.4860375842476704E-5	1.657358389498447E-5	0.2146714170977732	1.450188590811141E-5
78-79	0.13056047883072702	1.698792349235908E-4	2.4860375842476704E-5	1.657358389498447E-5	0.2157549151449078	1.657358389498447E-5
80-81	0.13062055807234635	1.7195093291046386E-4	2.4860375842476704E-5	1.657358389498447E-5	0.2165939528295914	2.4860375842476704E-5
82-83	0.13065784863611007	1.843811208317022E-4	2.6932073829349764E-5	1.657358389498447E-5	0.21753657541361865	2.6932073829349764E-5
84-85	0.1306992825958475	1.947396107660675E-4	2.900377181622282E-5	1.657358389498447E-5	0.21847919799764587	2.900377181622282E-5
86-87	0.13074071655558497	1.9681130875294056E-4	2.900377181622282E-5	1.657358389498447E-5	0.21934309605817193	2.900377181622282E-5
88-89	0.13077593542136184	1.9888300673981364E-4	2.900377181622282E-5	1.657358389498447E-5	0.22012619789721	3.314716778996894E-5
90-91	0.13079665240123056	2.0716979868730585E-4	2.900377181622282E-5	1.657358389498447E-5	0.22071870352145567	3.729056376371506E-5
92-93	0.13081944107908616	2.0716979868730585E-4	2.900377181622282E-5	1.657358389498447E-5	0.2212304129242133	3.729056376371506E-5
94-95	0.1308567316428499	2.1131319466105198E-4	2.900377181622282E-5	1.657358389498447E-5	0.22205080532701504	3.729056376371506E-5
96-97	0.13086087503882363	2.154565906347981E-4	2.900377181622282E-5	1.657358389498447E-5	0.22270546189086693	3.729056376371506E-5
98-99	0.13088159201869234	2.154565906347981E-4	3.107546980309588E-5	1.657358389498447E-5	0.2233621901527057	3.729056376371506E-5
100-101	0.13092095428044292	2.2374338258229033E-4	3.314716778996894E-5	1.657358389498447E-5	0.22392776370312203	3.729056376371506E-5
102-103	0.1309395995623248	2.2788677855603646E-4	3.314716778996894E-5	1.657358389498447E-5	0.224482978763604	3.729056376371506E-5
104-105	0.13098103352206225	2.2788677855603646E-4	3.314716778996894E-5	2.0716979868730585E-5	0.2251148466496003	4.764905369808034E-5
106-107	0.13100382219991785	2.3203017452978257E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22568663529397726	5.386414765869952E-5
108-109	0.13104111276368158	2.46532060437894E-4	3.314716778996894E-5	2.0716979868730585E-5	0.2262604956363411	5.386414765869952E-5
110-111	0.13108461842140592	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22688614842837676	5.593584564557258E-5
112-113	0.13108669011939278	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.227584310649953	5.800754363244564E-5
114-115	0.1311011920053009	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22819960495205427	5.800754363244564E-5
116-117	0.13112190898516962	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.2286595219051401	5.800754363244564E-5
118-119	0.13112812407913024	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.2292230237575696	5.800754363244564E-5
120-121	0.13113848256906463	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22990461239525078	6.629433557993787E-5
122-123	0.13115505615295958	2.527471543985131E-4	3.314716778996894E-5	2.0716979868730585E-5	0.23038317463021848	6.629433557993787E-5
124-125	0.1311716297368546	2.631056443328784E-4	3.314716778996894E-5	2.0716979868730585E-5	0.2309114576168711	7.0437731553684E-5
126-127	0.13121099199860517	2.7346413426724376E-4	3.314716778996894E-5	2.0716979868730585E-5	0.2315122500330643	7.0437731553684E-5
128-129	0.13123170897847392	2.7760753024098987E-4	3.5218865776842E-5	2.0716979868730585E-5	0.23220626885866677	7.872452350117623E-5
130-131	0.13125449765632952	2.858943221884821E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23300594428159976	8.079622148804929E-5
132-133	0.13128764482411948	2.900377181622282E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23366474424142541	8.286791947492234E-5
134-135	0.13131043350197508	2.900377181622282E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23437533665092286	8.286791947492234E-5
136-137	0.13132493538788317	2.9418111413597435E-4	3.729056376371506E-5	2.0716979868730585E-5	0.2350196347248404	8.286791947492234E-5
138-139	0.13132493538788317	2.9418111413597435E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23570122336252164	8.701131544866846E-5
>>END_MODULE
