##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.S.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035747
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	69-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.50003035062734	36.0	34.0	38.0	31.0	39.0
2	34.84493192576873	35.0	34.0	38.0	31.0	39.0
3	34.94975621103018	35.0	34.0	38.0	31.0	39.0
4	34.960591738629965	36.0	34.0	38.0	31.0	39.0
5	34.82875984673994	35.0	34.0	37.0	31.0	39.0
6	34.817214293360635	35.0	34.0	37.0	32.0	39.0
7	34.83127314495364	35.0	34.0	37.0	32.0	39.0
8	34.98005662149797	35.0	34.0	37.0	32.0	39.0
9	34.93901820484298	35.0	34.0	37.0	32.0	39.0
10-14	34.923253752005294	35.2	34.0	37.4	31.6	39.0
15-19	34.93652944508028	35.0	34.0	37.0	31.4	39.0
20-24	34.92864037884905	35.0	34.0	37.0	31.0	39.0
25-29	34.93020959157209	35.0	34.0	37.0	31.0	39.0
30-34	34.90722105703643	35.0	34.0	37.0	31.0	39.0
35-39	34.89799050555824	35.0	34.0	37.0	31.0	39.0
40-44	34.85389802114326	35.0	34.0	37.0	31.0	39.0
45-49	34.7502059494968	35.0	34.0	37.0	31.0	39.0
50-54	34.72700667052287	35.0	34.0	37.0	31.0	39.0
55-59	34.71796560348218	35.0	34.0	37.0	31.0	39.0
60-64	34.68919852584569	35.0	34.0	37.0	31.0	39.0
65-69	34.65698472362851	35.0	34.0	37.0	31.0	39.0
70-74	34.65590648889183	35.0	34.0	37.0	31.0	39.0
75-79	34.64045541245494	35.0	34.0	37.0	30.8	39.0
80-84	34.619426449985625	35.0	34.0	37.0	30.0	39.0
85-89	34.60598099424595	35.0	34.0	37.0	30.0	39.0
90-94	34.58114324622801	35.0	34.0	37.0	30.0	39.0
95-99	34.558816863285074	35.0	34.0	37.0	30.0	39.0
100-104	34.519393234014665	35.0	34.0	37.0	30.0	39.0
105-109	34.487618450572086	35.0	34.0	37.0	30.0	39.0
110-114	34.4590620561352	35.0	34.0	37.0	30.0	39.0
115-119	34.421477093751534	35.0	34.0	37.0	30.0	39.0
120-124	34.37689657040933	35.0	34.0	37.0	30.0	39.0
125-129	34.35648842845376	35.0	34.0	37.0	30.0	39.0
130-134	34.31171645357005	35.0	34.0	37.0	30.0	39.0
135-139	34.27124771469535	35.0	34.0	37.0	29.8	39.0
140-144	34.2274288550174	35.0	34.0	37.0	29.0	39.0
145-149	34.26296063712485	35.0	34.0	37.0	29.4	39.0
150	35.13185214397898	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	6.0
22	197.0
23	2530.0
24	17280.0
25	71424.0
26	188068.0
27	353894.0
28	534838.0
29	705898.0
30	886052.0
31	1112140.0
32	1425303.0
33	1876610.0
34	2583183.0
35	3998138.0
36	8604580.0
37	1675597.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.903718740258	10.36980876858123	15.705890064494355	38.020582426666415
2	23.178478962361424	12.122013589859733	35.751177397724796	28.948330050054043
3	22.243142650335486	16.99482241273933	27.764172255877085	32.9978626810481
4	27.474270802199126	24.527357773394673	21.648680664051447	26.34969076035475
5	27.120622127762754	30.247657381064652	23.67566403083812	18.95605646033447
6	20.179381339158443	35.89635876134697	25.10439253707461	18.819867362419984
7	17.09461940283449	24.126039104723947	39.578523043728886	19.200818448712674
8	19.597225028232998	23.67990968768795	30.777018222557572	25.945847061521476
9	20.362164127933653	22.63939143465774	32.00100833440223	24.997436103006375
10-14	21.268112472851932	28.38515770062286	26.526868492161586	23.819861334363623
15-19	21.522937114224465	27.355648940820217	27.14425898770303	23.977154957252292
20-24	21.426898972570438	27.405027479886275	27.069186380235834	24.098887167307453
25-29	21.38090970350623	27.606058772169956	26.714844953387317	24.298186570936494
30-34	21.388275610661054	27.379966128680227	26.976693060490003	24.255065200168715
35-39	21.70304955730955	27.097701571689687	26.865677652747337	24.333571218253425
40-44	21.925001373167042	27.183342043024755	26.648963095169513	24.242693488638686
45-49	22.183530698243345	27.169581072033615	26.538125304604222	24.10876292511882
50-54	22.137743407964543	27.417198934259385	26.435361787332063	24.009695870444006
55-59	22.04538827284061	27.35344445711818	26.53347604545544	24.06769122458577
60-64	22.269415179891155	27.17868108779578	26.293766395458647	24.258137336854418
65-69	22.070663894137546	27.31458483942776	26.510750421395635	24.104000845039064
70-74	22.465128242670655	27.034690718270486	26.434797156622526	24.06538388243633
75-79	22.313989132633324	26.929238334079614	26.410874469443442	24.345898063843624
80-84	22.593515022961927	26.924525217481083	26.45258305691256	24.029376702644427
85-89	22.438834436881542	26.91457353969941	26.4523873539746	24.194204669444446
90-94	22.41732349429625	26.66434988401719	26.468439073070815	24.449887548615738
95-99	22.65809265291907	26.70092450053094	26.646118234834677	23.99486461171531
100-104	22.598960323274213	26.524126558417542	26.6829493236646	24.193963794643643
105-109	22.67574084273246	26.501405579253788	26.581116479831362	24.241737098182387
110-114	22.625167138264068	26.567389289331363	26.56862580959625	24.23881776280832
115-119	22.709879026575823	26.753352095375448	26.494174861312768	24.042594016735958
120-124	22.678610923491462	26.614710643427646	26.470600056420007	24.236078376660885
125-129	23.079142712942595	26.572584035123008	26.37808225187725	23.97019100005714
130-134	22.77699749617996	26.836590648287117	26.29467895531198	24.091732900220943
135-139	22.882524477486132	26.643026842040037	26.36601600753744	24.10843267293639
140-144	22.81479841683171	26.47431793635423	26.666904422622068	24.043979224191993
145-149	22.24576764961125	26.704851064615102	26.918559608434716	24.130821677338936
150	22.038339863987584	25.2287934696602	27.606027328231626	25.126839338120593
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	2.0
9	2.0
10	0.5
11	3.5
12	6.5
13	11.5
14	17.0
15	46.0
16	87.0
17	183.5
18	453.5
19	798.5
20	1276.0
21	2104.5
22	3593.5
23	5913.5
24	9491.0
25	14706.5
26	21180.5
27	30982.5
28	47734.0
29	70939.5
30	97234.0
31	123078.0
32	150015.0
33	181907.0
34	216685.5
35	262333.5
36	327720.0
37	397452.0
38	466478.0
39	566976.0
40	705668.5
41	859200.0
42	1019257.5
43	1177134.0
44	1318873.5
45	1434733.5
46	1523977.5
47	1555969.0
48	1517747.0
49	1474591.5
50	1397292.5
51	1250451.5
52	1069494.0
53	912329.0
54	772688.5
55	645819.5
56	557075.0
57	460895.0
58	380466.5
59	319238.5
60	248875.5
61	174940.0
62	104598.5
63	59696.5
64	37924.5
65	23748.0
66	15553.5
67	7575.5
68	3425.0
69	1977.0
70	1421.0
71	1182.0
72	1074.0
73	772.0
74	422.5
75	143.0
76	47.5
77	38.5
78	18.5
79	6.0
80	4.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013563131613924875
3	0.1246934409818842
4	7.904892658422473E-5
5	1.7057926262911656E-4
6	1.0401174550555887E-4
7	1.3313503424711536E-4
8	1.7890020226956125E-4
9	2.912328874155648E-4
10-14	2.4463562542907446E-4
15-19	3.037142968762319E-4
20-24	3.927483510289903E-4
25-29	3.261808339054326E-4
30-34	3.037142968762319E-4
35-39	1.4728063163587136E-4
40-44	2.895686994874759E-4
45-49	2.0469511515493986E-4
50-54	1.2398200064262616E-4
55-59	1.689150747010276E-4
60-64	2.1468024272347348E-4
65-69	0.050933303633126106
70-74	1.930458269658027E-4
75-79	1.506090551139858E-4
80-84	1.2980674588017937E-4
85-89	1.1649333623168754E-4
90-94	1.3313549842053037E-4
95-99	9.485930862591723E-5
100-104	8.653869036672934E-5
105-109	1.0567753382585999E-4
110-114	7.072957481531864E-5
115-119	6.656959322159112E-5
120-124	1.1067302714261817E-4
125-129	1.0651373867874418E-4
130-134	5.991529425345506E-5
135-139	5.326056342552504E-5
140-144	5.078118317409617E-5
145-149	5.851981258224313E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	2.0
70-71	0.0
72-73	4.0
74-75	0.0
76-77	2.0
78-79	5.0
80-81	3.0
82-83	4.0
84-85	9.0
86-87	17.0
88-89	19.0
90-91	18.0
92-93	23.0
94-95	32.0
96-97	24.0
98-99	45.0
100-101	50.0
102-103	41.0
104-105	43.0
106-107	46.0
108-109	66.0
110-111	82.0
112-113	101.0
114-115	73.0
116-117	72.0
118-119	104.0
120-121	100.0
122-123	99.0
124-125	129.0
126-127	142.0
128-129	202.0
130-131	251.0
132-133	287.0
134-135	414.0
136-137	737.0
138-139	2006.0
140-141	5256.0
142-143	8125.0
144-145	67219.0
146-147	762167.0
148-149	1350457.0
150-151	2.1837271E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.8210540125044
#Duplication Level	Percentage of total
1	33.70248906978389
2	8.408673515177055
3	4.950518510360719
4	3.5278317977160674
5	2.563004503859303
6	2.0198105969041626
7	1.658133415860159
8	1.4536775260990311
9	1.2004262682940903
>10	15.827782043940939
>50	5.271322820073851
>100	10.54577528149924
>500	3.808956976847808
>1k	4.46050749082058
>5k	0.4860422587023727
>10k+	0.11504792406056849
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2481409460667064E-5	0.0	0.0	2.745910081346754E-4	1.664187928088942E-5
2	4.160469820222355E-6	2.912328874155648E-5	0.0	8.32093964044471E-6	0.0023090607502234068	2.496281892133413E-5
3	2.0802349101111773E-5	3.744422838200119E-5	0.0	1.2481409460667064E-5	0.004455863177458142	4.57651680224459E-5
4	6.656751712355768E-5	3.744422838200119E-5	0.0	1.2481409460667064E-5	0.00650281432900754	5.408610766289061E-5
5	7.488845676400239E-5	4.1604698202223546E-5	0.0	1.2481409460667064E-5	0.007663585408849577	5.824657748311296E-5
6	1.0817221532578122E-4	4.1604698202223546E-5	0.0	1.2481409460667064E-5	0.00936521756532052	5.824657748311296E-5
7	1.5393738334822712E-4	7.488845676400239E-5	0.0	1.2481409460667064E-5	0.010875468110061235	5.824657748311296E-5
8	2.4130724957289657E-4	7.904892658422473E-5	0.0	1.664187928088942E-5	0.014116474100014448	6.240704730333532E-5
9	3.0787476669645424E-4	7.904892658422473E-5	0.0	1.664187928088942E-5	0.016933112168304985	6.240704730333532E-5
10-11	3.7236204890990076E-4	9.361057095500297E-5	0.0	2.0802349101111776E-5	0.029537255488668604	7.488845676400239E-5
12-13	4.118865122020131E-4	9.569080586511416E-5	0.0	2.496281892133413E-5	0.037849874189472874	1.0817221532578122E-4
14-15	4.4309003585368074E-4	1.102524502358924E-4	0.0	2.496281892133413E-5	0.04379310532766051	1.2273385969655947E-4
16-17	5.200587275277944E-4	1.1857338987633711E-4	0.0	2.496281892133413E-5	0.0476457003811864	1.2481409460667064E-4
18-19	6.095088286625749E-4	1.3105479933700416E-4	0.0	2.496281892133413E-5	0.050990718116645184	1.2481409460667064E-4
20-21	9.236243000893627E-4	1.5185714843811594E-4	0.0	2.496281892133413E-5	0.05369710373469982	1.2689432951678182E-4
22-23	0.001243980476246484	1.560176182583383E-4	0.0	2.7043053831445305E-5	0.0563910079432938	1.3313503424711536E-4
24-25	0.0013521526915722653	1.5809785316844946E-4	0.0	2.912328874155648E-5	0.05912443661517988	1.372955040673377E-4
26-27	0.0014374423228868235	1.5809785316844946E-4	0.0	3.120352365166766E-5	0.061493824177796516	1.456164437077824E-4
28-29	0.0015414540683923823	1.6225832298867184E-4	0.0	3.744422838200119E-5	0.06409411781543548	1.4977691352800477E-4
30-31	0.001759878733954056	1.6225832298867184E-4	0.0	6.656751712355767E-5	0.06712293984455736	1.6017808807856064E-4
32-33	0.0018846928285607266	1.6225832298867184E-4	0.0	7.904892658422473E-5	0.06988757204009512	1.830606720897836E-4
34-35	0.0019887045740662854	1.6225832298867184E-4	0.0	1.0193151059544769E-4	0.07240257604641953	1.830606720897836E-4
36-37	0.002026148802448287	1.6641879280889418E-4	0.0	1.0817221532578122E-4	0.07458474246712615	1.8722114191000597E-4
38-39	0.0020407104468190647	1.7057926262911653E-4	0.0	1.1233268514600358E-4	0.0772911280851808	1.8930137682011714E-4
40-41	0.002088555849751622	1.7057926262911653E-4	0.0	1.1441292005611475E-4	0.07973124363474121	1.9554208155045068E-4
42-43	0.0020968767893920666	1.7890020226956125E-4	0.0	1.2273385969655947E-4	0.0821505568352005	2.0386302119089538E-4
44-45	0.0021135186686729564	1.830606720897836E-4	0.0	1.2481409460667064E-4	0.0847924551710417	2.0594325610100655E-4
46-47	0.002134321017774068	1.830606720897836E-4	0.0	1.2481409460667064E-4	0.08867625374821927	2.0802349101111772E-4
48-49	0.0021530431319650685	1.830606720897836E-4	0.0	1.2481409460667064E-4	0.09116421470071223	2.121839608313401E-4
50-51	0.0021655245414257357	1.8722114191000597E-4	0.0	1.28974564426893E-4	0.09396421088972189	2.1634443065156244E-4
52-53	0.0021988082999875144	1.913816117302283E-4	0.0	1.28974564426893E-4	0.09646465325167553	2.1634443065156244E-4
54-55	0.002240412998189738	1.913816117302283E-4	0.0	1.3105479933700416E-4	0.0981350818844948	2.184246655616736E-4
56-57	0.0023028200454930734	1.9554208155045068E-4	0.0	1.3521526915722653E-4	0.10002809565269596	2.288258401122295E-4
58-59	0.002369387562616631	2.0802349101111772E-4	0.0	1.3937573897744888E-4	0.10204800375041391	2.288258401122295E-4
60-61	0.002500442361953635	2.2050490047178479E-4	0.0	1.4145597388756005E-4	0.10383700577310953	2.3090607502234068E-4
62-63	0.002567009879077193	2.2050490047178479E-4	0.0	1.4145597388756005E-4	0.10612318393932171	2.3298630993245185E-4
64-65	0.002594052932908638	2.2258513538189599E-4	0.0	1.476966786178936E-4	0.10883373002719657	2.3298630993245185E-4
66-67	0.0026086145772794165	2.392270146627854E-4	0.0	1.5393738334822712E-4	0.11098053245443132	2.3298630993245185E-4
68-69	0.002635657631110862	2.5378865903356363E-4	0.0	1.5809785316844946E-4	0.11281737988005949	2.371467797526742E-4
70-71	0.002677262329313085	2.57949128853786E-4	0.0	1.6641879280889418E-4	0.11469375176897975	2.4546771939311894E-4
72-73	0.002714706557695086	2.57949128853786E-4	0.0	1.6641879280889418E-4	0.1163225757035968	2.5378865903356363E-4
74-75	0.0027417496115265316	2.6002936376389715E-4	0.0	1.7057926262911653E-4	0.11796804151749475	2.5378865903356363E-4
76-77	0.0027604717257175325	2.6418983358411955E-4	0.0	1.7265949753922773E-4	0.11930563256469624	2.5378865903356363E-4
78-79	0.0027937554842793113	2.7043053831445307E-4	0.0	2.017827862807842E-4	0.1206016189136955	2.5378865903356363E-4
80-81	0.002816638068290534	2.766712430447866E-4	0.0	2.0386302119089538E-4	0.12191840761179587	2.6002936376389715E-4
82-83	0.002847841591942202	2.891526525054536E-4	0.0	2.121839608313401E-4	0.12347442332455905	2.621095986740083E-4
84-85	0.0028915265250545365	2.953933572357872E-4	0.0	2.1426419574145127E-4	0.1251656543064794	2.6418983358411955E-4
86-87	0.002958094042178094	3.037142968762319E-4	0.0	2.1634443065156244E-4	0.12652820817260224	2.662700684942307E-4
88-89	0.0029955382705600954	3.0579453178634306E-4	0.0	2.2050490047178479E-4	0.1279178050925565	2.662700684942307E-4
90-91	0.0030038592102005404	3.0787476669645424E-4	0.0	2.2050490047178479E-4	0.1298711456731509	2.662700684942307E-4
92-93	0.0030413034385825414	3.0787476669645424E-4	0.0	2.2050490047178479E-4	0.13173919662243075	2.7251077322456424E-4
94-95	0.003107870955706099	3.0787476669645424E-4	0.0	2.2050490047178479E-4	0.13308094813945248	2.7875147795489776E-4
96-97	0.0032098024663015467	3.099550016065654E-4	0.0	2.2050490047178479E-4	0.1344497427103056	2.7875147795489776E-4
98-99	0.0032867711579756603	3.120352365166766E-4	0.0	2.3090607502234068E-4	0.13559179167595664	2.8083171286500893E-4
100-101	0.003413665487492442	3.1619570633689893E-4	0.0	2.3298630993245185E-4	0.13669015570849535	2.829119477751201E-4
102-103	0.003453189950784554	3.203561761571213E-4	0.0	2.3298630993245185E-4	0.1377989209155846	2.829119477751201E-4
104-105	0.0034802330046159995	3.203561761571213E-4	0.0	2.3506654484256302E-4	0.13878495226297732	2.829119477751201E-4
106-107	0.003544720286829446	3.3283758561778836E-4	0.0	2.3714677975267422E-4	0.13975642196599924	2.829119477751201E-4
108-109	0.003588405219941781	3.3283758561778836E-4	0.0	2.392270146627854E-4	0.14084438482398737	2.829119477751201E-4
110-111	0.0036736948512563388	3.3283758561778836E-4	0.0	2.4130724957289657E-4	0.14182833593646996	2.849921826852313E-4
112-113	0.0037631449523911195	3.3491782052789954E-4	0.0	2.4130724957289657E-4	0.14276028117619977	2.8707241759534245E-4
114-115	0.003794348476042787	3.390782903481219E-4	0.0	2.4546771939311894E-4	0.14386072544364858	2.912328874155648E-4
116-117	0.0038442741138854557	3.4323876016834423E-4	0.0	2.5378865903356363E-4	0.14485507773068174	2.912328874155648E-4
118-119	0.003942045154660681	3.494794648986778E-4	0.0	2.558688939436748E-4	0.14577454156095088	2.912328874155648E-4
120-121	0.004052297604896574	3.494794648986778E-4	0.0	2.57949128853786E-4	0.14662535763918633	2.912328874155648E-4
122-123	0.004227037337345913	3.494794648986778E-4	0.0	2.621095986740083E-4	0.14740128526065782	2.912328874155648E-4
124-125	0.0043144072035705815	3.494794648986778E-4	0.0	2.621095986740083E-4	0.1482167373454214	2.9747359214589837E-4
126-127	0.004374734015963807	3.5363993471890015E-4	0.0	2.621095986740083E-4	0.14905923248401642	3.037142968762319E-4
128-129	0.00443506082835703	3.5363993471890015E-4	0.0	2.683503034043419E-4	0.14974987047417332	3.037142968762319E-4
130-131	0.0045224306945816995	3.5988063944923367E-4	0.0	2.7043053831445307E-4	0.15044258869924032	3.037142968762319E-4
132-133	0.00459523891643559	3.64041109269456E-4	0.0	2.745910081346754E-4	0.15113738715921748	3.0579453178634306E-4
134-135	0.004682608782660261	3.702818139997896E-4	0.0	2.7875147795489776E-4	0.1519778020629024	3.099550016065654E-4
136-137	0.004784540293255708	3.827632234604566E-4	0.0	2.829119477751201E-4	0.15277245179856486	3.120352365166766E-4
138-139	0.0047887007630759305	3.827632234604566E-4	0.0	2.829119477751201E-4	0.15349221307746333	3.1619570633689893E-4
>>END_MODULE
