##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035747
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	58-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02940495254838	37.0	35.0	38.0	33.0	39.0
2	35.37488816137064	35.0	34.0	37.0	33.0	39.0
3	35.47329288330419	35.0	34.0	37.0	33.0	39.0
4	35.55120874753757	36.0	35.0	38.0	33.0	39.0
5	35.46969008285867	36.0	35.0	38.0	33.0	39.0
6	35.418264928483396	35.0	35.0	37.0	33.0	39.0
7	35.463845288436424	35.0	34.0	38.0	33.0	39.0
8	35.59122381343089	36.0	35.0	38.0	33.0	39.0
9	35.59206422833457	36.0	35.0	38.0	33.0	39.0
10-14	35.54170382971663	35.8	35.0	37.8	33.0	39.0
15-19	35.51106631302118	36.0	35.0	38.0	33.0	39.0
20-24	35.48896207802487	36.0	35.0	38.0	33.0	39.0
25-29	35.47935344801225	36.0	34.8	38.0	33.0	39.0
30-34	35.46694226728215	36.0	34.4	38.0	33.0	39.0
35-39	35.43589999511976	36.0	34.0	38.0	33.0	39.0
40-44	35.4250502803179	36.0	34.0	38.0	33.0	39.0
45-49	35.39514236857294	36.0	34.0	38.0	32.8	39.0
50-54	35.38110834666383	36.0	34.0	38.0	32.0	39.0
55-59	35.34842485521757	36.0	34.0	38.0	32.0	39.0
60-64	35.313098956862	36.0	34.0	38.0	32.0	39.0
65-69	35.276360599549136	36.0	34.0	38.0	32.0	39.0
70-74	35.260089309671116	36.0	34.0	38.0	32.0	39.0
75-79	35.20311804859168	36.0	34.0	38.0	32.0	39.0
80-84	35.13369363028691	36.0	34.0	38.0	32.0	39.0
85-89	35.11662801863235	36.0	34.0	38.0	32.0	39.0
90-94	35.07113087910835	36.0	34.0	38.0	32.0	39.0
95-99	35.00615779476045	35.8	34.0	38.0	31.0	39.0
100-104	34.947135737623704	35.4	34.0	38.0	31.0	39.0
105-109	34.89437176529009	35.0	34.0	38.0	31.0	39.0
110-114	34.82441969034694	35.0	34.0	37.8	31.0	39.0
115-119	34.76147242960375	35.0	34.0	37.0	30.6	39.0
120-124	34.69311594230046	35.0	34.0	37.0	30.0	39.0
125-129	34.616169882064	35.0	34.0	37.0	30.0	39.0
130-134	34.53935267992971	35.0	34.0	37.0	30.0	39.0
135-139	34.46106087974444	35.0	34.0	37.0	30.0	39.0
140-144	34.37388048441788	35.0	34.0	37.0	29.4	39.0
145-149	34.339634960130454	35.0	34.0	37.0	29.2	39.0
150	35.14035353028688	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	182.0
22	1557.0
23	8321.0
24	28768.0
25	71627.0
26	144243.0
27	251703.0
28	372792.0
29	491214.0
30	628274.0
31	813149.0
32	1080946.0
33	1498000.0
34	2228894.0
35	3763593.0
36	1.0203991E7
37	2448474.0
38	11.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.66592683805501	18.387691466381302	15.696308502498383	32.25007319306531
2	24.23835631153881	25.194469720454286	32.51232840818303	18.054845559823875
3	20.215781103037905	29.101221609630024	29.70348289986577	20.979514387466303
4	23.815673380153317	33.95764650043953	21.3376393086514	20.889040810755745
5	25.602324737400505	34.865739766690005	21.479012073142556	18.05292342276693
6	19.226175912069635	38.82522977130688	21.768127281419627	20.180467035203854
7	20.638809353418473	20.60491816626294	36.60557751752005	22.150694962798532
8	21.694998703389583	23.345240736641138	27.883389686203635	27.076370873765647
9	23.662455758083993	23.53653913897496	27.333051059324266	25.467954043616785
10-14	23.159096324320604	28.793460839806645	25.044400741944905	23.003042093927846
15-19	23.2244040511826	27.632079002994995	26.569306125580372	22.574210820242033
20-24	23.10495136474104	27.55188861965178	26.53320321416464	22.809956801442535
25-29	23.218556926897257	27.115134803174623	26.508921898703626	23.157386371224494
30-34	23.051150438553044	26.878667844190574	26.736204204512553	23.333977512743832
35-39	23.362295334528195	26.58650966828699	26.806345565211682	23.244849431973137
40-44	23.55655099881023	26.23721492824833	26.812242615134867	23.393991457806575
45-49	23.452653250177747	26.052165551584476	26.88636804173384	23.608813156503935
50-54	23.654951102622274	26.118338656169083	26.56541275792261	23.661297483286038
55-59	23.698399086147802	26.125770304752923	26.629627334899368	23.546203274199907
60-64	23.386345487258854	26.06957820239904	26.68693703120344	23.85713927913866
65-69	23.59947628665913	26.009342152931197	26.709716550322444	23.681465010087226
70-74	23.772371954146355	25.98592715751158	26.410811912053706	23.83088897628836
75-79	23.57213817936261	26.113757411889075	26.69718599904194	23.616918409706372
80-84	23.921589361992655	26.106697028852516	26.36494677384875	23.60676683530607
85-89	23.601969977407972	26.20669378965979	26.622340613417748	23.568995619514485
90-94	23.634107435726534	25.931178694252438	26.670945323557827	23.7637685464632
95-99	23.61452517559868	26.118210126885778	26.716780733825935	23.550483963689615
100-104	23.8740603153434	25.885837956823337	26.64586434903712	23.594237378796144
105-109	23.67976926884249	26.118437410877505	26.508257482470153	23.693535837809847
110-114	23.779361935399756	26.238231751503083	26.394220842901284	23.58818547019588
115-119	23.908732717068094	26.339734152785745	26.574333810674318	23.177199319471846
120-124	23.895941685941303	26.505216823548714	26.65776208373399	22.941079406775998
125-129	23.85184922585988	26.571665766663394	26.497633320109987	23.078851687366743
130-134	24.010735027641907	26.60679129320394	26.521271055661373	22.86120262349278
135-139	23.75786328960175	26.597990884344373	26.8164257448732	22.827720081180676
140-144	23.783746015735357	26.792230790127334	26.55136109991937	22.872662094217937
145-149	23.61357340200727	27.22643280353434	26.249446106532638	22.91054768792575
150	23.73538320608283	25.9446861788481	26.536980340981486	23.782950274087582
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	5.5
3	13.0
4	23.0
5	27.0
6	22.0
7	15.0
8	13.0
9	9.5
10	7.0
11	6.5
12	8.0
13	12.5
14	14.5
15	24.0
16	44.0
17	77.5
18	136.0
19	231.0
20	376.0
21	566.5
22	907.0
23	1509.5
24	2450.5
25	3948.0
26	6108.0
27	9554.5
28	14638.0
29	21024.0
30	29860.5
31	43115.5
32	62357.0
33	86851.5
34	119387.5
35	170980.5
36	243932.5
37	331476.0
38	442006.0
39	578957.5
40	741308.0
41	921751.0
42	1114583.0
43	1309200.0
44	1464268.0
45	1568418.5
46	1610448.0
47	1590188.5
48	1554961.5
49	1516801.0
50	1416493.5
51	1262009.0
52	1087109.0
53	920042.5
54	782322.5
55	671624.5
56	560056.5
57	446925.5
58	371303.0
59	309869.0
60	236552.5
61	160943.5
62	100219.0
63	61402.5
64	38285.5
65	19370.5
66	10871.5
67	6245.5
68	3902.5
69	2582.0
70	1329.0
71	1020.0
72	884.5
73	713.0
74	572.0
75	338.5
76	112.0
77	42.0
78	12.5
79	2.0
80	1.5
81	2.0
82	0.5
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.026157705853701987
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.320940471301229E-7
70-74	8.320941579110183E-7
75-79	5.059135258485741E-4
80-84	0.050467435997383396
85-89	8.320974467322835E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.321185235008743E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	2.49688345249668E-6
140-144	2.4650455705312995E-4
145-149	0.027044765249800304
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	0.0
62-63	0.0
64-65	1.0
66-67	1.0
68-69	1.0
70-71	1.0
72-73	3.0
74-75	6.0
76-77	6.0
78-79	15.0
80-81	13.0
82-83	18.0
84-85	25.0
86-87	20.0
88-89	26.0
90-91	27.0
92-93	39.0
94-95	45.0
96-97	73.0
98-99	72.0
100-101	88.0
102-103	76.0
104-105	99.0
106-107	100.0
108-109	121.0
110-111	110.0
112-113	123.0
114-115	161.0
116-117	172.0
118-119	163.0
120-121	206.0
122-123	278.0
124-125	234.0
126-127	337.0
128-129	352.0
130-131	493.0
132-133	631.0
134-135	847.0
136-137	1388.0
138-139	4573.0
140-141	8600.0
142-143	10131.0
144-145	61025.0
146-147	786540.0
148-149	1059183.0
150-151	2.2099323E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.665824805315644
#Duplication Level	Percentage of total
1	24.251744725026157
2	8.386763070318938
3	5.159748119098749
4	3.6162397048446238
5	2.805895760982869
6	2.182713690251096
7	1.8212355853729396
8	1.5244957728469317
9	1.3614486546655487
>10	17.124229422113192
>50	6.059692688859803
>100	13.086500385364753
>500	4.307668741289068
>1k	6.270089300755322
>5k	1.1932778285193422
>10k+	0.8482565496905197
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	33825	0.14072789166902114	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	24077	0.10017163186149362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.3283758561778836E-4	0.0	0.0	0.0	0.002849921826852313	0.0
2	0.0016017808807856065	8.32093964044471E-6	0.0	0.0	0.011695080664645039	0.0
3	0.0024796400128525233	8.32093964044471E-6	0.0	0.0	0.016741730556574755	0.0
4	0.005999397480760635	8.32093964044471E-6	0.0	0.0	0.02522908898982836	0.0
5	0.006199100032131309	8.32093964044471E-6	0.0	0.0	0.031265930698970996	0.0
6	0.007343229232692456	8.32093964044471E-6	0.0	0.0	0.03986146134755038	0.0
7	0.009456747901365413	8.32093964044471E-6	0.0	0.0	0.054834992230530635	0.0
8	0.01606357397587851	8.32093964044471E-6	0.0	0.0	0.07447657025180036	0.0
9	0.01653786753538386	1.2481409460667064E-5	0.0	0.0	0.09034876261594865	0.0
10-11	0.01720562294152955	1.2481409460667064E-5	0.0	0.0	0.1510812208166445	0.0
12-13	0.018064759959405464	1.2481409460667064E-5	2.0802349101111775E-6	0.0	0.17979678351581918	0.0
14-15	0.021590758132043912	1.2481409460667064E-5	4.160469820222355E-6	0.0	0.192255310392475	0.0
16-17	0.025358063554255253	1.2481409460667064E-5	4.160469820222355E-6	0.0	0.20145202893007652	0.0
18-19	0.0330154082583745	1.4561644370778242E-5	4.160469820222355E-6	0.0	0.20914681786257777	0.0
20-21	0.0394370934258877	2.496281892133413E-5	4.160469820222355E-6	0.0	0.21425171433199058	0.0
22-23	0.04199578236532445	2.912328874155648E-5	4.160469820222355E-6	0.0	0.2193753329155944	0.0
24-25	0.048313455787332094	3.744422838200119E-5	4.160469820222355E-6	2.0802349101111775E-6	0.22395601018765923	0.0
26-27	0.05389680628607049	4.57651680224459E-5	4.160469820222355E-6	4.160469820222355E-6	0.22761930386436502	0.0
28-29	0.06767420209573682	4.992563784266826E-5	4.160469820222355E-6	4.160469820222355E-6	0.2311889869701158	0.0
30-31	0.09435321481791267	6.032681239322414E-5	4.160469820222355E-6	4.160469820222355E-6	0.23533073467614715	0.0
32-33	0.11515348368411434	7.072798694378003E-5	4.160469820222355E-6	4.160469820222355E-6	0.2387776839222014	0.0
34-35	0.13004172493578003	7.072798694378003E-5	4.160469820222355E-6	4.160469820222355E-6	0.24249090323674982	0.0
36-37	0.13254424753264377	7.072798694378003E-5	8.32093964044471E-6	4.160469820222355E-6	0.2448124453964339	0.0
38-39	0.1350238875454963	7.696869167411356E-5	8.32093964044471E-6	6.240704730333532E-6	0.24660352765403962	0.0
40-41	0.1365695020837089	8.320939640444709E-5	1.2481409460667064E-5	8.32093964044471E-6	0.24820322829991512	0.0
42-43	0.13693354319297835	8.736986622466945E-5	1.2481409460667064E-5	8.32093964044471E-6	0.24992150233566696	0.0
44-45	0.1378592477279778	8.945010113478062E-5	1.2481409460667064E-5	8.32093964044471E-6	0.2512362107988572	0.0
46-47	0.1395900031731903	9.569080586511416E-5	1.2481409460667064E-5	8.32093964044471E-6	0.2527693439276092	0.0
48-49	0.1412396294569085	9.777104077522534E-5	1.2481409460667064E-5	8.32093964044471E-6	0.25390931265835004	2.0802349101111775E-6
50-51	0.1425272948662673	1.0817221532578122E-4	1.2481409460667064E-5	8.32093964044471E-6	0.254993115046518	8.32093964044471E-6
52-53	0.143588214670424	1.1233268514600358E-4	1.2481409460667064E-5	8.32093964044471E-6	0.2561996512943825	8.32093964044471E-6
54-55	0.1440687489346597	1.2065362478644828E-4	1.2481409460667064E-5	8.32093964044471E-6	0.25725849086362906	8.32093964044471E-6
56-57	0.14499237323474906	1.2065362478644828E-4	1.2481409460667064E-5	8.32093964044471E-6	0.25831733043287564	8.32093964044471E-6
58-59	0.14518791531629952	1.2065362478644828E-4	1.2481409460667064E-5	8.32093964044471E-6	0.2593304048340998	1.0401174550555888E-5
60-61	0.14556651806993975	1.28974564426893E-4	1.664187928088942E-5	8.32093964044471E-6	0.26006056728754884	1.2481409460667064E-5
62-63	0.14585775095735531	1.28974564426893E-4	2.0802349101111773E-5	8.32093964044471E-6	0.2609488275941663	1.2481409460667064E-5
64-65	0.1458951951857373	1.352152691572265E-4	2.0802349101111773E-5	8.32093964044471E-6	0.26175179826946926	1.2481409460667064E-5
66-67	0.1460054476359732	1.456164437077824E-4	2.0802349101111773E-5	8.32093964044471E-6	0.26246531884363733	1.664187928088942E-5
68-69	0.14607409538800686	1.456164437077824E-4	2.0802349101111773E-5	8.32093964044471E-6	0.26326828951894027	1.664187928088942E-5
70-71	0.14618226760333264	1.456164437077824E-4	2.496281892133413E-5	8.32093964044471E-6	0.2640150938516702	1.664187928088942E-5
72-73	0.1462321932411753	1.5393738334822712E-4	2.496281892133413E-5	8.32093964044471E-6	0.2645185106999171	1.664187928088942E-5
74-75	0.1462654769997371	1.5809785316844946E-4	2.496281892133413E-5	8.32093964044471E-6	0.26510721717947855	1.664187928088942E-5
76-77	0.14631332240266964	1.6849902771900536E-4	2.496281892133413E-5	8.32093964044471E-6	0.2658311389281972	2.0802349101111773E-5
78-79	0.14638821085943365	1.747397324493389E-4	2.496281892133413E-5	8.32093964044471E-6	0.26694198437019656	2.288258401122295E-5
80-81	0.14642357485290555	1.8098043717967242E-4	2.496281892133413E-5	1.0401174550555888E-5	0.2676804677632861	2.496281892133413E-5
82-83	0.146450617906737	1.830606720897836E-4	2.496281892133413E-5	1.2481409460667064E-5	0.2683856673978138	2.496281892133413E-5
84-85	0.146488062135119	1.8930137682011714E-4	2.496281892133413E-5	1.2481409460667064E-5	0.26931345216772334	3.120352365166766E-5
86-87	0.14651510518895045	1.9346184664033948E-4	2.496281892133413E-5	1.2481409460667064E-5	0.2701580275412285	3.328375856177884E-5
88-89	0.14655254941733242	1.9554208155045066E-4	2.496281892133413E-5	1.2481409460667064E-5	0.27096723892126173	3.744422838200119E-5
90-91	0.14659623435044478	1.9970255137067303E-4	2.496281892133413E-5	1.2481409460667064E-5	0.2715060197629805	4.1604698202223546E-5
92-93	0.14660871575990544	1.9970255137067303E-4	2.496281892133413E-5	1.2481409460667064E-5	0.272094726242542	4.1604698202223546E-5
94-95	0.14664199951846724	2.0594325610100655E-4	2.496281892133413E-5	1.2481409460667064E-5	0.2728373701054517	4.57651680224459E-5
96-97	0.14664407975337734	2.0802349101111772E-4	2.496281892133413E-5	1.2481409460667064E-5	0.27346352081339514	4.57651680224459E-5
98-99	0.1466711228072088	2.121839608313401E-4	2.496281892133413E-5	1.2481409460667064E-5	0.27403766564858584	4.784540293255708E-5
100-101	0.14672312867996157	2.184246655616736E-4	2.496281892133413E-5	1.2481409460667064E-5	0.2745889278997653	4.992563784266826E-5
102-103	0.14675433220361322	2.2258513538189599E-4	2.496281892133413E-5	1.2481409460667064E-5	0.27515059132549535	4.992563784266826E-5
104-105	0.14677097408289413	2.2466537029200716E-4	2.496281892133413E-5	1.2481409460667064E-5	0.27576634085888824	4.992563784266826E-5
106-107	0.1468042578414559	2.288258401122295E-4	2.496281892133413E-5	1.2481409460667064E-5	0.2762593565325846	4.992563784266826E-5
108-109	0.14683338113019745	2.288258401122295E-4	2.496281892133413E-5	1.664187928088942E-5	0.2768189397234045	4.992563784266826E-5
110-111	0.14685626371420868	2.3506654484256302E-4	2.496281892133413E-5	1.664187928088942E-5	0.2773265170414716	5.616634257300179E-5
112-113	0.1468791462982199	2.413072495728966E-4	2.496281892133413E-5	1.664187928088942E-5	0.27785905717846004	5.824657748311296E-5
114-115	0.14690618935205135	2.496281892133413E-4	2.496281892133413E-5	1.664187928088942E-5	0.27844360318820127	6.240704730333532E-5
116-117	0.14692075099642213	2.496281892133413E-4	2.912328874155648E-5	1.664187928088942E-5	0.278942859566628	6.240704730333532E-5
118-119	0.14693947311061312	2.5170842412345246E-4	2.912328874155648E-5	1.664187928088942E-5	0.279504522992358	6.240704730333532E-5
120-121	0.14695819522480413	2.558688939436748E-4	2.912328874155648E-5	1.664187928088942E-5	0.2801161120559307	6.240704730333532E-5
122-123	0.14696443592953445	2.57949128853786E-4	2.912328874155648E-5	1.664187928088942E-5	0.28062993007872816	6.240704730333532E-5
124-125	0.14697483710408502	2.57949128853786E-4	2.912328874155648E-5	1.664187928088942E-5	0.28118743303463795	6.240704730333532E-5
126-127	0.14699771968809622	2.621095986740083E-4	2.912328874155648E-5	1.664187928088942E-5	0.28180318256803083	6.44872822134465E-5
128-129	0.14701436156737713	2.6627006849423067E-4	2.912328874155648E-5	1.664187928088942E-5	0.2824542960948957	7.072798694378003E-5
130-131	0.14703932438629846	2.849921826852313E-4	2.912328874155648E-5	1.664187928088942E-5	0.2831740573737941	7.280822185389121E-5
132-133	0.14705804650048945	2.9331312232567597E-4	2.912328874155648E-5	1.664187928088942E-5	0.28375236267880505	7.488845676400239E-5
134-135	0.14708925002414114	2.9747359214589837E-4	2.912328874155648E-5	1.664187928088942E-5	0.2842100143590295	7.488845676400239E-5
136-137	0.1471017314336018	3.016340619661207E-4	2.912328874155648E-5	1.664187928088942E-5	0.28483616506697296	7.488845676400239E-5
138-139	0.1471017314336018	3.120352365166766E-4	2.912328874155648E-5	1.664187928088942E-5	0.2854581553050962	7.488845676400239E-5
>>END_MODULE
