##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24115250
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.39960456557572	36.0	34.0	38.0	31.0	39.0
2	34.724242087475766	35.0	34.0	37.0	31.0	39.0
3	34.836442126869926	35.0	34.0	38.0	31.0	39.0
4	34.832371300318265	35.0	34.0	38.0	31.0	39.0
5	34.707583375664775	35.0	34.0	37.0	31.0	39.0
6	34.70274428007174	35.0	34.0	37.0	31.0	39.0
7	34.728050341588826	35.0	34.0	37.0	32.0	39.0
8	34.85110492323322	35.0	34.0	37.0	32.0	39.0
9	34.81801324887778	35.0	34.0	37.0	31.0	39.0
10-14	34.795540149905136	35.0	34.0	37.0	31.0	39.0
15-19	34.814677127543774	35.0	34.0	37.0	31.0	39.0
20-24	34.801809875493724	35.0	34.0	37.0	31.0	39.0
25-29	34.79639448067094	35.0	34.0	37.0	31.0	39.0
30-34	34.78223890691575	35.0	34.0	37.0	31.0	39.0
35-39	34.77238284488032	35.0	34.0	37.0	31.0	39.0
40-44	34.72979993157857	35.0	34.0	37.0	31.0	39.0
45-49	34.63184943137641	35.0	34.0	37.0	31.0	39.0
50-54	34.60563416924975	35.0	34.0	37.0	31.0	39.0
55-59	34.591830306548765	35.0	34.0	37.0	31.0	39.0
60-64	34.56811620032967	35.0	34.0	37.0	30.4	39.0
65-69	34.53090137354711	35.0	34.0	37.0	30.0	39.0
70-74	34.53317461147765	35.0	34.0	37.0	30.0	39.0
75-79	34.51656605086244	35.0	34.0	37.0	30.0	39.0
80-84	34.50290161488848	35.0	34.0	37.0	30.0	39.0
85-89	34.483768035664866	35.0	34.0	37.0	30.0	39.0
90-94	34.4596002118834	35.0	34.0	37.0	30.0	39.0
95-99	34.44352820125525	35.0	34.0	37.0	30.0	39.0
100-104	34.40114826431343	35.0	34.0	37.0	30.0	39.0
105-109	34.37626155092742	35.0	34.0	37.0	30.0	39.0
110-114	34.33955922743898	35.0	34.0	37.0	30.0	39.0
115-119	34.30265893203782	35.0	34.0	37.0	30.0	39.0
120-124	34.259553169967866	35.0	34.0	37.0	30.0	39.0
125-129	34.241960584570116	35.0	34.0	37.0	30.0	39.0
130-134	34.18594588209947	35.0	34.0	37.0	29.2	39.0
135-139	34.14979818327299	35.0	34.0	37.0	29.0	39.0
140-144	34.099013954287926	35.0	34.0	37.0	29.0	39.0
145-149	34.13262759384651	35.0	34.0	37.0	29.2	39.0
150	35.01723255726531	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	11.0
22	238.0
23	2762.0
24	19328.0
25	80280.0
26	208104.0
27	380983.0
28	564101.0
29	739300.0
30	929464.0
31	1167435.0
32	1493095.0
33	1963606.0
34	2701454.0
35	4199729.0
36	8582903.0
37	1082441.0
38	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.02344574491245	10.975697950467028	15.674931837737532	37.32592446688299
2	22.86255209972627	12.814689396267273	36.03646887535088	28.286289628655588
3	21.951991321215736	17.598673096287946	27.844331783243874	32.605003799252444
4	26.747093549194645	25.18468717516647	22.116718156242875	25.95150111939601
5	26.099866814501272	30.82744611519074	24.286299807615418	18.78638726269258
6	19.662556084354467	36.483340387656156	25.70817363312664	18.14592989486274
7	16.309502424110633	25.031440704125806	40.105591336530985	18.55346553523258
8	18.80503653479413	24.651316837893862	31.44128704386037	25.102359583451637
9	19.990365423472895	23.41198778680375	32.391003410417824	24.206643379305532
10-14	20.517060542009766	29.39376547886653	27.017074603669567	23.07209937545414
15-19	20.67262091104469	28.059534139201975	27.81883876601343	23.449006183739904
20-24	20.87878361516184	28.214707099427798	27.51257391760059	23.393935367809767
25-29	20.86189259234701	28.40415689534351	27.405470962329208	23.328479549980262
30-34	20.83137170795887	28.146860532727775	27.433305773958555	23.5884619853548
35-39	20.87960697028537	28.123321262812183	27.490576596320377	23.50649517058207
40-44	20.936001417329983	28.200370763624676	27.38668322540501	23.476944593640322
45-49	21.054139821162167	27.963320975821617	27.356526363020638	23.626012839995578
50-54	21.1549487607154	27.955499188035855	27.429100668567614	23.460451382681136
55-59	21.141680553469506	27.98307807408947	27.384542427185615	23.49069894525541
60-64	21.092867387727676	27.917736047350044	27.34898945909809	23.640407105824192
65-69	21.193768194773714	27.821010265256557	27.45013464356617	23.53508689640356
70-74	21.32177250156217	27.740180830703405	27.435849082517127	23.502197585217303
75-79	21.370190307877007	27.64740430725212	27.415071248110912	23.567334136759957
80-84	21.384758165915155	27.661436046476044	27.389624938173856	23.564180849434948
85-89	21.33942456649696	27.675170910793767	27.471214752485434	23.514189770223844
90-94	21.32701301524769	27.580179856821253	27.416061214453073	23.676745913477983
95-99	21.42536281766375	27.481831509536335	27.52557271423966	23.567232958560258
100-104	21.507925494519437	27.547278016619643	27.369313255400613	23.575483233460307
105-109	21.46749320846895	27.384170092952942	27.484627327008372	23.663709371569734
110-114	21.531099978462784	27.363965710045377	27.541365752748252	23.563568558743587
115-119	21.55187580091598	27.39124354311644	27.519082398066725	23.537798257900857
120-124	21.522024486034724	27.30811783903382	27.51284353345914	23.657014141472317
125-129	21.568435792370575	27.238095587237076	27.568290911146637	23.625177709245712
130-134	21.630706339602742	27.346622949100986	27.4675222396983	23.555148471597974
135-139	21.61125877204224	27.22249830019195	27.567641660140747	23.598601267625064
140-144	21.662913891293236	27.14046316674828	27.689196512744374	23.50742642921411
145-149	21.30494511200487	27.36880760328336	27.726252431892934	23.599994852818835
150	21.278724000373444	25.946840094962052	28.37522328990712	24.399212614757378
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	1.0
11	7.0
12	12.0
13	16.5
14	33.5
15	81.0
16	176.0
17	366.5
18	678.5
19	1226.5
20	2355.0
21	3967.0
22	6174.0
23	9796.5
24	15500.0
25	24151.5
26	35811.5
27	50325.5
28	68007.5
29	94316.5
30	127369.0
31	162022.5
32	194935.5
33	231191.0
34	271846.0
35	319983.0
36	393213.0
37	491100.5
38	609791.0
39	756188.0
40	921399.5
41	1085962.0
42	1254984.0
43	1424802.0
44	1563437.5
45	1645134.0
46	1663926.5
47	1624689.5
48	1538166.0
49	1410478.5
50	1247672.0
51	1072604.0
52	896038.0
53	720582.0
54	558670.0
55	424276.5
56	320068.5
57	240001.0
58	179872.5
59	132737.5
60	92733.0
61	63901.0
62	45449.0
63	31333.5
64	21991.5
65	16966.5
66	14408.0
67	10606.5
68	6434.5
69	4415.5
70	3528.5
71	2733.0
72	2160.5
73	1395.0
74	708.0
75	238.0
76	66.5
77	39.0
78	20.0
79	3.5
80	1.5
81	1.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018245715885176394
3	0.1581779164636485
4	5.3907796933475704E-5
5	4.976104332320835E-5
6	5.3907796933475704E-5
7	5.8054550543743064E-5
8	7.878831859507988E-5
9	9.537533303614933E-5
10-14	1.3020806336239518E-4
15-19	1.5674728646810628E-4
20-24	2.197779413441702E-4
25-29	1.7001689802096184E-4
30-34	1.8245715885176393E-4
35-39	1.0366884025668404E-4
40-44	1.9572677040461946E-4
45-49	1.625527415224806E-4
50-54	9.371663159204237E-5
55-59	1.1610910108748613E-4
60-64	1.9075066607229866E-4
65-69	0.06415442882890289
70-74	2.0153226390140735E-4
75-79	1.3269617165490513E-4
80-84	1.119624412619116E-4
85-89	8.376458687712136E-5
90-94	1.2274444130441677E-4
95-99	8.791193990359693E-5
100-104	7.132518663686468E-5
105-109	9.869472903382879E-5
110-114	3.732187110662906E-5
115-119	4.3127980889925316E-5
120-124	7.962198137310098E-5
125-129	1.0948191333909384E-4
130-134	2.820059220580089E-5
135-139	3.8985456740795745E-5
140-144	3.9830435859978694E-5
145-149	4.906909289529703E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	1.0
68-69	1.0
70-71	1.0
72-73	4.0
74-75	1.0
76-77	1.0
78-79	6.0
80-81	4.0
82-83	4.0
84-85	15.0
86-87	18.0
88-89	14.0
90-91	34.0
92-93	36.0
94-95	47.0
96-97	44.0
98-99	50.0
100-101	68.0
102-103	56.0
104-105	44.0
106-107	73.0
108-109	75.0
110-111	109.0
112-113	105.0
114-115	108.0
116-117	110.0
118-119	131.0
120-121	144.0
122-123	148.0
124-125	136.0
126-127	171.0
128-129	220.0
130-131	284.0
132-133	358.0
134-135	512.0
136-137	895.0
138-139	2594.0
140-141	5700.0
142-143	9031.0
144-145	74777.0
146-147	785138.0
148-149	1394100.0
150-151	2.1839881E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.817416656555324
#Duplication Level	Percentage of total
1	41.644330386534435
2	10.151837317126835
3	6.341183519212888
4	4.533260661023348
5	3.329255069777235
6	2.6001357877662703
7	2.1804526899588152
8	1.8600940587420882
9	1.5324317401381131
>10	17.24266571267706
>50	4.0706786029736115
>100	3.887571373488748
>500	0.32023535472775566
>1k	0.3058677258526094
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.9027275271871532E-5	0.0	4.146753610267362E-6	2.197779413441702E-4	4.976104332320834E-5
2	4.146753610267362E-6	9.122857942588196E-5	0.0	8.293507220534723E-6	0.0020111755009796705	9.122857942588196E-5
3	8.293507220534723E-6	1.0781559386695141E-4	0.0	1.2440260830802086E-5	0.003172266511854532	1.5757663719015975E-4
4	2.488052166160417E-5	1.202558546977535E-4	0.0	1.2440260830802086E-5	0.00465265755071998	1.617233908004271E-4
5	2.9027275271871532E-5	1.2440260830802086E-4	0.0	1.2440260830802086E-5	0.005963031691564467	1.7001689802096184E-4
6	4.561428971294098E-5	1.2440260830802086E-4	0.0	1.2440260830802086E-5	0.007182177252983071	1.741636516312292E-4
7	5.3907796933475704E-5	1.409896227490903E-4	0.0	1.2440260830802086E-5	0.008359855278299001	1.741636516312292E-4
8	6.634805776427779E-5	1.4928312996962504E-4	0.0	1.2440260830802086E-5	0.010603248981453645	1.8245715885176393E-4
9	8.70818258156146E-5	1.534298835798924E-4	0.0	1.2440260830802086E-5	0.01414872331823224	1.9489741968256602E-4
10-11	1.4720975316449134E-4	1.6172339080042713E-4	0.0	1.2440260830802086E-5	0.0188801691875473	2.46731839810908E-4
12-13	2.5087859342117543E-4	1.7209027482609552E-4	0.0	1.2440260830802086E-5	0.022867272783819368	2.9441950632898273E-4
14-15	2.6331885425197744E-4	1.8660391246203128E-4	0.0	1.2440260830802086E-5	0.027376867334985122	3.504006800675921E-4
16-17	3.151532743803195E-4	2.0526430370823443E-4	0.0	1.6587014441069446E-5	0.03185536123407387	3.607675640932605E-4
18-19	3.566208104829931E-4	2.1148443412363546E-4	0.0	1.6587014441069446E-5	0.035738795990089264	3.6284094089839415E-4
20-21	4.437026362986077E-4	2.5709872383657644E-4	0.0	1.6587014441069446E-5	0.039520635282653094	3.773545785343299E-4
22-23	5.287110853090886E-4	2.6331885425197744E-4	0.0	2.280714485647049E-5	0.04361555447279211	3.8979483936513203E-4
24-25	6.406734327863075E-4	2.7161236147251226E-4	0.0	2.488052166160417E-5	0.0481085620095168	3.8979483936513203E-4
26-27	7.67149417899462E-4	2.7575911508277956E-4	0.0	2.488052166160417E-5	0.0523009299094971	4.208954914421372E-4
28-29	8.480111132996755E-4	2.7783249188791326E-4	0.0	3.1100652077005216E-5	0.05674417640289858	4.478493899088751E-4
30-31	9.496065767512259E-4	2.7783249188791326E-4	0.0	4.146753610267362E-5	0.06097593846217643	4.7687666518074663E-4
32-33	0.00101388125771037	2.819792454981806E-4	0.0	4.7687666518074666E-5	0.06508537128995137	5.328578389193559E-4
34-35	0.001071935808254113	2.819792454981806E-4	0.0	5.3907796933475704E-5	0.06882159629280227	5.598117373860939E-4
36-37	0.0011113299675516528	2.819792454981806E-4	0.0	6.634805776427779E-5	0.07246659271622728	5.743253750220296E-4
38-39	0.0011445039964337919	2.8819937591358167E-4	0.0	7.464156498481252E-5	0.0767771430941002	5.846922590476979E-4
40-41	0.001202558546977535	2.902727527187153E-4	0.0	7.464156498481252E-5	0.08042628627113549	5.888390126579654E-4
42-43	0.0012212189382237381	2.9234612952384897E-4	0.0	7.464156498481252E-5	0.0838058904635034	6.095727807093022E-4
44-45	0.0012854936191828822	3.0271301354951744E-4	0.0	7.464156498481252E-5	0.0878572687407346	6.157929111247032E-4
46-47	0.0013601351841676947	3.047863903546511E-4	0.0	7.464156498481252E-5	0.09173033661272431	6.365266791760401E-4
48-49	0.0014098962274909032	3.089331439649185E-4	0.0	7.464156498481252E-5	0.09575890774509906	6.572604472273769E-4
50-51	0.0014327033723473734	3.1100652077005215E-4	0.0	7.464156498481252E-5	0.0999720094131307	6.7592083847358E-4
52-53	0.0015425923430194585	3.1100652077005215E-4	0.0	7.464156498481252E-5	0.10403997470480297	6.7592083847358E-4
54-55	0.001656628067301811	3.172266511854532E-4	0.0	7.464156498481252E-5	0.1072723691440064	6.7592083847358E-4
56-57	0.0016939488497942174	3.3174028882138897E-4	0.0	7.878831859507988E-5	0.11117653766807312	6.987279833300505E-4
58-59	0.0017374897627020246	3.524740568727258E-4	0.0	8.500844901048092E-5	0.11502057826479095	7.21535128186521E-4
60-61	0.0018121313276868371	3.5662081048299315E-4	0.0	9.122857942588196E-5	0.11840225583396398	7.256818817967883E-4
62-63	0.001857745617399778	3.607675640932605E-4	0.0	9.122857942588196E-5	0.12184613470729103	7.298286354070557E-4
64-65	0.001903359907112719	3.6284094089839415E-4	0.0	9.952208664641669E-5	0.12610692404184076	7.298286354070557E-4
66-67	0.0019323871823845908	3.773545785343299E-4	0.0	9.952208664641669E-5	0.13002767958034853	7.298286354070557E-4
68-69	0.002040202776251542	3.8979483936513203E-4	0.0	9.952208664641669E-5	0.1330050486725205	7.360487658224567E-4
70-71	0.002170825514974964	4.022351001959341E-4	0.0	1.0781559386695141E-4	0.1357315391712713	7.401955194327242E-4
72-73	0.002241320326349509	4.1882211463700357E-4	0.0	1.0781559386695141E-4	0.13878147645162295	7.464156498481252E-4
74-75	0.0023055950073086534	4.208954914421372E-4	0.0	1.181824778926198E-4	0.14193093581862098	7.547091570686598E-4
76-77	0.002361576181047263	4.271156218575383E-4	0.0	1.223292315028872E-4	0.1443692269414582	7.547091570686598E-4
78-79	0.0024258508620064066	4.4162925949347404E-4	0.0	1.389162459439566E-4	0.14692777391899314	7.547091570686598E-4
80-81	0.002471465151719348	4.478493899088751E-4	0.0	1.4720975316449137E-4	0.14950705466457948	7.567825338737936E-4
82-83	0.002527446325457957	4.561428971294098E-4	0.0	1.4928312996962504E-4	0.15217341723598138	7.588559106789272E-4
84-85	0.0026497755569608442	4.6236302754481087E-4	0.0	1.4928312996962504E-4	0.15464488238770072	7.588559106789272E-4
86-87	0.0027783249188791325	4.644364043499445E-4	0.0	1.534298835798924E-4	0.15687376245321943	7.609292874840609E-4
88-89	0.0028550398606690787	4.644364043499445E-4	0.0	1.5757663719015975E-4	0.15910471589554326	7.630026642891945E-4
90-91	0.00288406713594095	4.665097811550782E-4	0.0	1.5757663719015975E-4	0.16195768237940722	7.67149417899462E-4
92-93	0.002931754802459025	4.706565347653456E-4	0.0	1.5757663719015975E-4	0.16486455666020466	7.67149417899462E-4
94-95	0.0030063963674438372	4.727299115704793E-4	0.0	1.5757663719015975E-4	0.16791242056375116	7.67149417899462E-4
96-97	0.003097624946869719	4.7687666518074663E-4	0.0	1.5757663719015975E-4	0.1704149863675475	7.67149417899462E-4
98-99	0.0032406879464239435	4.8517017240128134E-4	0.0	1.6172339080042713E-4	0.17247384953504524	7.67149417899462E-4
100-101	0.003358870424316563	4.93463679621816E-4	0.0	1.6587014441069449E-4	0.17442489710867604	7.67149417899462E-4
102-103	0.003387897699588435	4.93463679621816E-4	0.0	1.6794352121582816E-4	0.17622458817553208	7.67149417899462E-4
104-105	0.003431438612496242	5.017571868423508E-4	0.0	1.741636516312292E-4	0.17800769222794705	7.67149417899462E-4
106-107	0.003516447061506723	5.141974476731529E-4	0.0	1.741636516312292E-4	0.17973896186023366	7.692227947045957E-4
108-109	0.003618042524958273	5.141974476731529E-4	0.0	1.741636516312292E-4	0.18155523994153078	7.712961715097294E-4
110-111	0.0037258581188252247	5.183442012834203E-4	0.0	1.741636516312292E-4	0.18317869397995046	7.712961715097294E-4
112-113	0.003815013321445973	5.266377085039549E-4	0.0	1.741636516312292E-4	0.18490996361223708	7.712961715097294E-4
114-115	0.0038855081328205182	5.307844621142223E-4	0.0	1.8038378204663025E-4	0.18679673650490874	7.712961715097294E-4
116-117	0.003974663335441267	5.328578389193559E-4	0.0	1.9489741968256602E-4	0.18860679445579043	7.712961715097294E-4
118-119	0.0040741854220876835	5.494448533604255E-4	0.0	1.9489741968256602E-4	0.19009133224826613	7.712961715097294E-4
120-121	0.004225541928862442	5.515182301655591E-4	0.0	1.9489741968256602E-4	0.19149708172214677	7.712961715097294E-4
122-123	0.004426659478960409	5.535916069706929E-4	0.0	1.9904417329283337E-4	0.1929505188625455	7.712961715097294E-4
124-125	0.004563502348099232	5.66031867801495E-4	0.0	2.0111755009796705E-4	0.194416396263775	7.712961715097294E-4
126-127	0.004607043261007039	5.701786214117623E-4	0.0	2.0319092690310073E-4	0.1958262924912659	7.712961715097294E-4
128-129	0.004669244565161049	5.763987518271633E-4	0.0	2.0319092690310073E-4	0.19734193093581862	7.754429251199967E-4
130-131	0.004764619898197198	5.805455054374306E-4	0.0	2.073376805133681E-4	0.19880780833704814	7.754429251199967E-4
132-133	0.004851701724012813	5.909123894630991E-4	0.0	2.2392469495443755E-4	0.19993365194223572	7.754429251199967E-4
134-135	0.004969884201905433	5.971325198785002E-4	0.0	2.280714485647049E-4	0.2010159546345155	7.754429251199967E-4
136-137	0.005156488114367464	5.971325198785002E-4	0.0	2.280714485647049E-4	0.20206093654430288	7.775163019251303E-4
138-139	0.005179295259223935	6.095727807093022E-4	0.0	2.280714485647049E-4	0.20310591845409026	7.795896787302641E-4
>>END_MODULE
