##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24119721
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	60-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.76595471398695	36.0	35.0	38.0	32.0	39.0
2	35.132548796895286	35.0	34.0	37.0	32.0	39.0
3	35.27182370807689	35.0	34.0	37.0	33.0	39.0
4	35.336815794842735	36.0	34.0	37.0	33.0	39.0
5	35.24736803547603	35.0	34.0	37.0	32.0	39.0
6	35.21137101876096	35.0	34.0	37.0	33.0	39.0
7	35.24522974374372	35.0	34.0	37.0	33.0	39.0
8	35.3819892029431	36.0	34.0	38.0	33.0	39.0
9	35.35623463472069	35.0	34.0	38.0	33.0	39.0
10-14	35.314850134460514	35.4	34.0	37.4	32.4	39.0
15-19	35.28408206711843	35.0	34.0	37.0	32.0	39.0
20-24	35.24911522815707	35.0	34.0	37.0	32.0	39.0
25-29	35.237548850585796	35.0	34.0	37.0	32.0	39.0
30-34	35.22018729818641	35.0	34.0	37.0	32.0	39.0
35-39	35.19267309103617	35.0	34.0	37.0	32.0	39.0
40-44	35.169256617852255	35.0	34.0	37.0	32.0	39.0
45-49	35.13890868803996	35.0	34.0	37.0	32.0	39.0
50-54	35.113042849873764	35.0	34.0	37.0	32.0	39.0
55-59	35.081005597038214	35.0	34.0	37.0	32.0	39.0
60-64	35.03777222083141	35.0	34.0	37.0	32.0	39.0
65-69	34.999264675915974	35.0	34.0	37.0	32.0	39.0
70-74	34.97409551772388	35.0	34.0	37.0	31.8	39.0
75-79	34.92290339817684	35.0	34.0	37.0	31.0	39.0
80-84	34.84211700072792	35.0	34.0	37.0	31.0	39.0
85-89	34.82691221081792	35.0	34.0	37.0	31.0	39.0
90-94	34.772618691071045	35.0	34.0	37.0	31.0	39.0
95-99	34.704737155566775	35.0	34.0	37.0	31.0	39.0
100-104	34.6338021479546	35.0	34.0	37.0	30.2	39.0
105-109	34.57476904682754	35.0	34.0	37.0	30.0	39.0
110-114	34.50588343889501	35.0	34.0	37.0	30.0	39.0
115-119	34.44638489576657	35.0	34.0	37.0	30.0	39.0
120-124	34.368944482674536	35.0	34.0	37.0	30.0	39.0
125-129	34.28464723655831	35.0	34.0	37.0	29.6	39.0
130-134	34.20517410395462	35.0	34.0	37.0	29.0	39.0
135-139	34.11352433795812	35.0	34.0	37.0	29.0	39.0
140-144	34.01521330917228	35.0	34.0	37.0	28.4	39.0
145-149	33.98645312078147	35.0	34.0	37.0	28.2	39.0
150	34.863483535467104	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	205.0
22	2015.0
23	10560.0
24	35594.0
25	89070.0
26	178241.0
27	306961.0
28	445870.0
29	579777.0
30	735831.0
31	946306.0
32	1245387.0
33	1706963.0
34	2514461.0
35	4187845.0
36	9776059.0
37	1358561.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.04392285466321	18.67220188823909	16.64734844984318	31.636526807254526
2	24.03140567007388	25.250661066933567	33.713926458767915	17.00400680422464
3	19.649493458071092	29.35082458043358	30.408535820128268	20.59114614136706
4	23.193485529952852	34.19860868208218	22.388281356985846	20.219624430979113
5	24.953837567192423	35.25266730904557	22.60172495361783	17.191770170144174
6	18.69850816267734	38.76602055222778	23.182225034858405	19.353246250236477
7	20.07483834493774	20.30741566206342	38.50609631844415	21.111649674554695
8	21.334023722745382	22.96908824111191	28.630853565843484	27.066034470299222
9	23.36709864927542	23.148994965571948	28.825337573349213	24.65856881180342
10-14	23.026495206971923	28.964582965118048	25.841505380597063	22.167416447312966
15-19	22.851672289244142	28.147857929202413	27.279774919452844	21.720694862100604
20-24	22.94506885047734	28.26780958191128	27.119163436845138	21.66795813076624
25-29	23.0535203951986	28.26162292673286	27.0306526348294	21.65420404323914
30-34	23.053363676967905	28.0511768772118	27.202050968997526	21.69340847682276
35-39	23.13996666876868	28.042995190533095	27.176568916365163	21.640469224333067
40-44	23.300559736988667	27.809987520170736	27.2720509495114	21.6174017933292
45-49	23.308698305424013	27.65166562250036	27.350418356829252	21.689217715246375
50-54	23.386688815955853	27.63945366763494	27.29345712238474	21.680400394024467
55-59	23.545378489245376	27.531290266583103	27.19268270142926	21.730648542742266
60-64	23.47054175197273	27.50524775988073	27.253327756264767	21.770882731881773
65-69	23.56161831679157	27.42240978808143	27.223196012875373	21.79277588225162
70-74	23.627341650698604	27.404022107168856	27.125124836723753	21.84351140540879
75-79	23.55111586625214	27.441882923075273	27.20870796680672	21.798293243865867
80-84	23.662528731367058	27.484598047292202	27.082721718332547	21.770151503008194
85-89	23.696336504854862	27.39995277341194	27.13457208556067	21.76913863617252
90-94	23.626589909815337	27.349390458127683	27.192784397807074	21.831235234249906
95-99	23.706191659186597	27.466068660316555	27.17502657180552	21.65271310869133
100-104	23.853125704461174	27.42379650304074	26.99266471025218	21.730413082245907
105-109	23.64035073061941	27.562406306671722	27.087801660518373	21.709441302190495
110-114	23.678292687063045	27.5573780612928	27.02719673521256	21.737132516431597
115-119	23.755441569628395	27.565025648409254	27.043982717672467	21.635550064289884
120-124	23.729708720434108	27.468390226059768	27.2209312332248	21.58096982028132
125-129	23.706460032583134	27.535971819669502	27.185346513435483	21.572221634311877
130-134	23.749007394251905	27.466022990060807	27.25509206551794	21.529877550169353
135-139	23.680753220428198	27.4772349127006	27.33723299357298	21.504778873298225
140-144	23.6891194475763	27.592393411332022	27.324971866438467	21.39351527465321
145-149	23.395294569693664	27.602829347175	27.365742896244726	21.63613318688661
150	23.496942315979695	26.448418656853356	27.82692601433588	22.227713012831074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	13.0
3	31.5
4	41.5
5	41.5
6	33.5
7	20.5
8	13.0
9	13.0
10	12.5
11	10.5
12	13.5
13	19.5
14	30.5
15	44.0
16	77.5
17	160.0
18	245.0
19	395.0
20	618.0
21	981.5
22	1469.5
23	2407.0
24	4057.5
25	6700.5
26	10698.5
27	17146.5
28	28660.5
29	43944.5
30	59547.0
31	78255.5
32	103771.0
33	136575.5
34	184425.5
35	255167.5
36	354486.0
37	480670.5
38	629129.0
39	812579.0
40	1023908.5
41	1223297.5
42	1409641.5
43	1582036.0
44	1699668.5
45	1750827.5
46	1747640.5
47	1687528.0
48	1578281.5
49	1430296.0
50	1240677.0
51	1038015.5
52	846128.0
53	671523.0
54	525156.0
55	399249.0
56	296727.5
57	220208.0
58	160692.5
59	115368.0
60	79345.5
61	52871.5
62	35947.5
63	25398.0
64	17712.5
65	12449.0
66	9307.0
67	7023.0
68	6202.0
69	4770.0
70	2728.5
71	1960.0
72	1526.5
73	1215.0
74	1039.0
75	643.5
76	220.5
77	69.5
78	21.0
79	2.5
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03452278739045116
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.6583939756185407E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	7.869094291376741E-4
80-84	0.06662547085556678
85-89	1.658411605040354E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.293406043061637E-7
130-134	0.0
135-139	1.6589968752627953E-6
140-144	4.274211534745783E-4
145-149	0.03689690756004066
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	2.0
66-67	1.0
68-69	2.0
70-71	10.0
72-73	7.0
74-75	13.0
76-77	17.0
78-79	27.0
80-81	34.0
82-83	48.0
84-85	58.0
86-87	68.0
88-89	96.0
90-91	92.0
92-93	97.0
94-95	104.0
96-97	112.0
98-99	146.0
100-101	177.0
102-103	161.0
104-105	171.0
106-107	200.0
108-109	180.0
110-111	184.0
112-113	193.0
114-115	242.0
116-117	219.0
118-119	266.0
120-121	297.0
122-123	356.0
124-125	347.0
126-127	469.0
128-129	534.0
130-131	659.0
132-133	933.0
134-135	1189.0
136-137	1837.0
138-139	3679.0
140-141	7423.0
142-143	12040.0
144-145	75542.0
146-147	843698.0
148-149	1183504.0
150-151	2.1984286E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.15042782439096
#Duplication Level	Percentage of total
1	29.82975098306646
2	10.650358885642202
3	7.083134906293289
4	5.014903805000444
5	3.8997223631020486
6	3.0003489119581133
7	2.558688889221031
8	2.130410665214816
9	1.874375889331386
>10	20.820997162289018
>50	5.152233712068467
>100	6.055944885916516
>500	0.9753098428181114
>1k	0.8547126981668054
>5k	0.05648089536863253
>10k+	0.04262550454286498
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	8.291969878092703E-6	0.0	0.0	0.0013515910901291106	0.0
2	8.291969878092703E-6	2.0729924695231757E-5	0.0	4.145984939046352E-6	0.005107853444905105	0.0
3	4.145984939046351E-5	2.0729924695231757E-5	0.0	4.145984939046352E-6	0.006633575902474162	0.0
4	9.950363853711243E-5	2.0729924695231757E-5	0.0	8.291969878092703E-6	0.007951999113090902	0.0
5	1.0779560841520513E-4	2.0729924695231757E-5	0.0	8.291969878092703E-6	0.00946528361584282	0.0
6	1.285255331104369E-4	2.0729924695231757E-5	0.0	8.291969878092703E-6	0.011127823576400407	0.0
7	1.451094728666223E-4	2.4875909634278106E-5	0.0	8.291969878092703E-6	0.013466159082022549	0.0
8	2.4875909634278106E-4	2.4875909634278106E-5	0.0	1.2437954817139053E-5	0.01883106359314853	0.0
9	2.7363500597705917E-4	2.902189457332446E-5	0.0	1.2437954817139053E-5	0.024871763649339063	0.0
10-11	2.8399996832467506E-4	3.5240871981893986E-5	0.0	1.2437954817139053E-5	0.030939412607633398	0.0
12-13	3.1094887042847633E-4	4.975181926855621E-5	0.0	1.2437954817139053E-5	0.03673135356748115	0.0
14-15	3.4411674994084716E-4	5.182481173807939E-5	0.0	1.2437954817139053E-5	0.04441800964447309	0.0
16-17	3.731386445141716E-4	5.597079667712574E-5	0.0	1.2437954817139053E-5	0.04973523532880003	0.0
18-19	4.332554261303437E-4	5.804378914664892E-5	0.0	1.2437954817139053E-5	0.05687254840136832	0.0
20-21	4.995911851550854E-4	6.426276655521845E-5	0.0	1.2437954817139053E-5	0.06291946743496743	0.0
22-23	5.65926944179827E-4	8.291969878092703E-5	2.072992469523176E-6	1.2437954817139053E-5	0.06905759813722555	0.0
24-25	6.384816806131381E-4	9.121166865901973E-5	4.145984939046352E-6	1.2437954817139053E-5	0.07400790415444689	0.0
26-27	6.737225525950321E-4	9.743064606758926E-5	4.145984939046352E-6	1.2437954817139053E-5	0.07789891101974189	0.0
28-29	7.918831233578532E-4	1.0364962347615879E-4	4.145984939046352E-6	1.2437954817139053E-5	0.08209050179311776	0.0
30-31	9.888174079625547E-4	1.0364962347615879E-4	4.145984939046352E-6	1.2437954817139053E-5	0.08704702678774767	0.0
32-33	0.0011733137377501176	1.1401458582377466E-4	4.145984939046352E-6	1.2437954817139053E-5	0.09197038390286522	0.0
34-35	0.001343299120251018	1.2023356323234419E-4	4.145984939046352E-6	1.2437954817139053E-5	0.0951814492381566	0.0
36-37	0.0014531677211357462	1.2230655570186737E-4	1.2437954817139053E-5	1.2437954817139053E-5	0.09861639776015652	0.0
38-39	0.0015153574952214415	1.2437954817139053E-4	1.2437954817139053E-5	1.2437954817139053E-5	0.10200781344029643	0.0
40-41	0.0015568173446119048	1.3681750298852959E-4	1.865693222570858E-5	1.2437954817139053E-5	0.10488098100305555	0.0
42-43	0.0015837662467157064	1.4718246533614547E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.10779768140767465	0.0
44-45	0.0016438830283318781	1.5754742768376136E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.11026661543887675	0.0
46-47	0.0016936348476004346	2.0937223942184074E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.11287858595047597	0.0
48-49	0.0017475326518080372	2.1559121683041027E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.1152874032000619	0.0
50-51	0.0018138684108327786	2.1559121683041027E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.11746819127800028	0.0
52-53	0.001876058184918474	2.2802917164754935E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.1196800742429815	0.0
54-55	0.001969342846047017	2.4046712646468837E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.12171989883299231	0.0
56-57	0.0020211676577850963	2.4875909634278106E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.12388617596364403	0.0
58-59	0.002083357431870792	2.653430360989665E-4	2.0729924695231757E-5	1.6583939756185407E-5	0.12574565020880632	0.0
60-61	0.0021890800478164734	2.6948902103801284E-4	2.0729924695231757E-5	1.6583939756185407E-5	0.12730454054588775	0.0
62-63	0.002253342814371692	2.7363500597705917E-4	2.0729924695231757E-5	1.6583939756185407E-5	0.1293775330154109	0.0
64-65	0.0022844377014145396	2.819269758551519E-4	2.0729924695231757E-5	2.0729924695231757E-5	0.13106287589313326	0.0
66-67	0.0023569924378478505	3.0265690055038366E-4	2.0729924695231757E-5	2.4875909634278106E-5	0.1323004523974386	0.0
68-69	0.002371503385134513	3.130218628979995E-4	2.2802917164754933E-5	2.4875909634278106E-5	0.13353802890174393	4.145984939046352E-6
70-71	0.0024005252797078373	3.275328101846617E-4	2.4875909634278106E-5	2.4875909634278106E-5	0.13478182438345782	4.145984939046352E-6
72-73	0.002444058121567824	3.4411674994084716E-4	2.902189457332446E-5	2.902189457332446E-5	0.135646262243249	4.145984939046352E-6
74-75	0.002475153008610672	3.461897424103703E-4	3.316787951237081E-5	2.902189457332446E-5	0.1367801891240782	6.2189774085695275E-6
76-77	0.0025103938805925655	3.503357273494167E-4	3.731386445141716E-5	2.902189457332446E-5	0.13827067070966537	8.291969878092703E-6
78-79	0.0025808756245563535	3.6070068969703254E-4	3.731386445141716E-5	2.902189457332446E-5	0.13999954642924767	1.0364962347615878E-5
80-81	0.002599532556782062	3.752116369836948E-4	3.731386445141716E-5	2.902189457332446E-5	0.1415750207060853	1.6583939756185407E-5
82-83	0.0026202624814772937	3.980145541484497E-4	4.3532841859986686E-5	2.902189457332446E-5	0.1433557212374057	1.6583939756185407E-5
84-85	0.0026327004362944333	4.063065240265424E-4	4.5605834329509866E-5	2.902189457332446E-5	0.14485242180040142	1.6583939756185407E-5
86-87	0.0026637953233372806	4.166714863741583E-4	4.5605834329509866E-5	2.902189457332446E-5	0.1466227573693742	1.6583939756185407E-5
88-89	0.0026783062706239426	4.2496345625225103E-4	4.975181926855621E-5	2.902189457332446E-5	0.14842626081785937	1.6583939756185407E-5
90-91	0.002703182180258221	4.477663734170059E-4	4.975181926855621E-5	2.902189457332446E-5	0.14940885924841335	1.865693222570858E-5
92-93	0.0027197661200144066	4.5191235835605225E-4	5.3897804207602566E-5	3.731386445141716E-5	0.15039974964884545	2.0729924695231757E-5
94-95	0.0027301310823620224	4.5605834329509864E-4	5.3897804207602566E-5	3.938685692094034E-5	0.1514507568308937	2.0729924695231757E-5
96-97	0.0027342770673010686	4.6227732070366814E-4	5.597079667712574E-5	4.5605834329509866E-5	0.1523836034421791	2.4875909634278106E-5
98-99	0.0027550069919963006	4.767882679903304E-4	5.804378914664892E-5	4.5605834329509866E-5	0.15322731137727505	2.902189457332446E-5
100-101	0.0027674449468134393	4.767882679903304E-4	5.804378914664892E-5	4.5605834329509866E-5	0.15402955946298053	2.902189457332446E-5
102-103	0.0027695179392829626	4.8093425292937674E-4	6.426276655521845E-5	4.5605834329509866E-5	0.15491887323240597	2.902189457332446E-5
104-105	0.002784028886569625	4.912992152769926E-4	6.633575902474163E-5	4.5605834329509866E-5	0.15574807022021525	2.902189457332446E-5
106-107	0.0028151237736124724	5.161751249112708E-4	6.840875149426479E-5	4.5605834329509866E-5	0.1564798365619569	2.902189457332446E-5
108-109	0.0028151237736124724	5.30686072197933E-4	7.048174396378797E-5	4.5605834329509866E-5	0.1572219678660462	2.902189457332446E-5
110-111	0.002827561728429612	5.514159968931648E-4	7.048174396378797E-5	4.5605834329509866E-5	0.15807396777102023	2.902189457332446E-5
112-113	0.002844145668185797	5.597079667712574E-4	7.048174396378797E-5	4.5605834329509866E-5	0.15888243483413428	2.902189457332446E-5
114-115	0.0028482916531248435	5.679999366493501E-4	7.048174396378797E-5	4.5605834329509866E-5	0.15980906246801113	2.902189457332446E-5
116-117	0.0028503646455943664	5.679999366493501E-4	7.462772890283432E-5	4.5605834329509866E-5	0.1607543470341137	2.902189457332446E-5
118-119	0.0028607296079419826	5.721459215883964E-4	7.462772890283432E-5	4.5605834329509866E-5	0.16155659511981918	2.902189457332446E-5
120-121	0.002864875592881029	5.742189140579197E-4	7.462772890283432E-5	4.5605834329509866E-5	0.16246041983653128	2.902189457332446E-5
122-123	0.002864875592881029	5.78364898996966E-4	7.462772890283432E-5	4.5605834329509866E-5	0.16311755844937012	3.316787951237081E-5
124-125	0.0028773135476981675	5.90802853814105E-4	8.49926912504502E-5	4.5605834329509866E-5	0.1638742007007461	3.731386445141716E-5
126-127	0.0028980434723933995	5.928758462836282E-4	8.706568371997337E-5	4.5605834329509866E-5	0.1648070473120315	4.145984939046351E-5
128-129	0.0029187733970886314	5.990948236921977E-4	8.913867618949655E-5	4.5605834329509866E-5	0.16582695960703692	4.145984939046351E-5
130-131	0.0029312113519057706	6.032408086312441E-4	9.121166865901973E-5	4.5605834329509866E-5	0.16687382080414612	4.3532841859986686E-5
132-133	0.002960233246479095	6.094597860398137E-4	9.121166865901973E-5	4.5605834329509866E-5	0.16771130976183346	4.767882679903304E-5
134-135	0.0029851091561133728	6.094597860398137E-4	9.121166865901973E-5	4.5605834329509866E-5	0.16846795201320947	5.3897804207602566E-5
136-137	0.0029934011259914657	6.094597860398137E-4	9.32846611285429E-5	4.5605834329509866E-5	0.16932824388806156	5.804378914664892E-5
138-139	0.002997547110930512	6.156787634483832E-4	9.535765359806608E-5	4.767882679903304E-5	0.17006001022980324	6.011678161617209E-5
>>END_MODULE
