##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24114212
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03239682889078	37.0	35.0	38.0	33.0	39.0
2	35.39004803474399	35.0	34.0	38.0	33.0	39.0
3	35.48580865922552	35.0	34.0	38.0	33.0	39.0
4	35.57658836208291	36.0	35.0	38.0	33.0	39.0
5	35.4990967981869	36.0	35.0	38.0	33.0	39.0
6	35.44234200976586	35.0	35.0	37.0	33.0	39.0
7	35.47981115866444	35.0	34.0	38.0	33.0	39.0
8	35.60821373719365	36.0	35.0	38.0	33.0	39.0
9	35.61093545167472	36.0	35.0	38.0	33.0	39.0
10-14	35.55837087274508	35.8	35.0	37.8	33.0	39.0
15-19	35.52949424181889	36.0	35.0	38.0	33.0	39.0
20-24	35.49737856663116	36.0	35.0	38.0	33.0	39.0
25-29	35.4943565147391	36.0	35.0	38.0	33.0	39.0
30-34	35.48033766145873	36.0	34.8	38.0	33.0	39.0
35-39	35.454022972013355	36.0	34.2	38.0	33.0	39.0
40-44	35.436962543084555	36.0	34.0	38.0	33.0	39.0
45-49	35.41186462987055	36.0	34.0	38.0	33.0	39.0
50-54	35.3916530965225	36.0	34.0	38.0	32.8	39.0
55-59	35.36360017901477	36.0	34.0	38.0	32.0	39.0
60-64	35.321504579954755	36.0	34.0	38.0	32.0	39.0
65-69	35.288535700682544	36.0	34.0	38.0	32.0	39.0
70-74	35.26994417018553	36.0	34.0	38.0	32.0	39.0
75-79	35.21497041587143	36.0	34.0	38.0	32.0	39.0
80-84	35.13434325580847	36.0	34.0	38.0	32.0	39.0
85-89	35.12831661547916	36.0	34.0	38.0	32.0	39.0
90-94	35.08258707476237	36.0	34.0	38.0	32.0	39.0
95-99	35.01682351422223	35.2	34.0	38.0	31.4	39.0
100-104	34.95324820163579	35.4	34.0	38.0	31.0	39.0
105-109	34.90150643006398	35.0	34.0	38.0	31.0	39.0
110-114	34.832876084165754	35.0	34.0	37.2	31.0	39.0
115-119	34.774293108971975	35.0	34.0	37.0	31.0	39.0
120-124	34.705684704908826	35.0	34.0	37.0	30.2	39.0
125-129	34.63003292649372	35.0	34.0	37.0	30.0	39.0
130-134	34.554373483600415	35.0	34.0	37.0	30.0	39.0
135-139	34.47525789307066	35.0	34.0	37.0	30.0	39.0
140-144	34.385771768964666	35.0	34.0	37.0	29.8	39.0
145-149	34.35328987069264	35.0	34.0	37.0	29.2	39.0
150	35.13507398634174	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	132.0
22	1301.0
23	7108.0
24	24891.0
25	63086.0
26	132330.0
27	240724.0
28	365226.0
29	488008.0
30	626420.0
31	813967.0
32	1083911.0
33	1507273.0
34	2247338.0
35	3827298.0
36	1.0403414E7
37	2281769.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.70464686965512	18.616498851382744	15.550609740015556	32.128244538946575
2	24.9804575808206	25.262838799197652	32.09875836695459	17.657945253027158
3	20.263747370223005	29.291519042795176	29.549976586421316	20.8947570005605
4	23.921507366693135	33.84033448822628	21.468435294505994	20.76972285057459
5	25.852837322654377	34.941602072669845	21.335787377169947	17.869773227505835
6	19.433067105821248	38.94745555027882	21.63981970466213	19.979657639237807
7	20.863780282921137	20.705798750786414	36.55800307959485	21.872417886697598
8	21.881639756671294	23.464656444091975	27.794571931274387	26.859131867962343
9	23.865652338131554	23.367174511031088	27.42186640807504	25.345306742762318
10-14	23.398546246491872	28.760439932770264	24.98974567354509	22.85126814719277
15-19	23.45488002601752	27.628146051434666	26.502670317448818	22.414303605098993
20-24	23.34263935795026	27.637549002448562	26.433487948831562	22.58632369076961
25-29	23.459834391436885	27.242775339289544	26.440881418808125	22.856508850465442
30-34	23.254600426127137	27.004146990199363	26.686934051064444	23.054318532609056
35-39	23.585201125377846	26.755720651373554	26.727212151904446	22.93186607134415
40-44	23.72975571418216	26.450250997212766	26.779109348462228	23.04088394014285
45-49	23.624617081616574	26.320198448286003	26.83693439235696	23.218250077740464
50-54	23.82685281194343	26.326036281011376	26.55912123522842	23.28798967181677
55-59	23.87034749466414	26.353649872531598	26.600711646725177	23.17529098607908
60-64	23.53424196486288	26.338456342674604	26.700875815473463	23.426425876989054
65-69	23.78275591391539	26.30502220383712	26.680409987631194	23.231811894616296
70-74	23.910087598727998	26.28703643212243	26.411674152972203	23.39120181617737
75-79	23.726403356468403	26.402991535353042	26.690795984585264	23.179809123593294
80-84	24.043090746075883	26.395996047679365	26.392090969773353	23.1688222364714
85-89	23.717448489679853	26.463318635332943	26.665712094514323	23.153520780472878
90-94	23.760730395204593	26.21337805658881	26.668074034492413	23.357817513714185
95-99	23.733628704164733	26.40272427107841	26.72461832956784	23.13902869518902
100-104	23.9896967251395	26.204785201747143	26.675667263315116	23.129850809798246
105-109	23.782829154501307	26.39492474705104	26.610519089299068	23.211727009148582
110-114	23.864924482293475	26.517195142589483	26.48831326553521	23.129567109581835
115-119	23.983935898597306	26.56533163051306	26.655831005151608	22.794901465738025
120-124	23.951152538065422	26.70479485164164	26.78765991160839	22.556392698684544
125-129	23.893814331017555	26.783172186750182	26.644809318804807	22.678204163427456
130-134	24.056928371229365	26.77273089082289	26.676742014782285	22.493598723165462
135-139	23.806877214922608	26.830774280150877	26.93479614938985	22.42755235553667
140-144	23.837073447678243	26.959377510840188	26.731802426343155	22.471746615138414
145-149	23.647617486820685	27.26182524353059	26.507229550822615	22.58332771882611
150	23.753271946948935	26.15689195551798	26.788258182420364	23.301577915112723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	5.0
2	14.5
3	36.5
4	50.0
5	45.0
6	36.0
7	24.0
8	19.5
9	13.5
10	7.5
11	8.5
12	10.5
13	11.0
14	14.0
15	21.5
16	36.5
17	63.5
18	128.0
19	231.5
20	341.5
21	524.5
22	870.0
23	1471.0
24	2442.0
25	4027.0
26	6370.5
27	9854.5
28	15190.5
29	21997.5
30	31487.5
31	45726.5
32	66022.5
33	91195.5
34	123754.5
35	175148.0
36	248287.0
37	337723.0
38	450748.5
39	591142.5
40	757831.5
41	944690.5
42	1144835.0
43	1342178.5
44	1499684.0
45	1600365.0
46	1634879.0
47	1613020.5
48	1574754.0
49	1532857.0
50	1431689.0
51	1272079.0
52	1081922.5
53	898762.5
54	757688.0
55	641923.0
56	525816.5
57	422271.0
58	350688.5
59	284746.0
60	212146.5
61	141980.5
62	88570.5
63	53917.5
64	33074.0
65	17989.0
66	10720.5
67	6168.0
68	3660.0
69	2344.5
70	1380.0
71	1196.5
72	1067.0
73	884.0
74	738.5
75	429.0
76	131.5
77	49.5
78	15.5
79	3.0
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.29386421584085E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	4.146932107920425E-6
8	0.0
9	0.0
10-14	8.293864215840849E-7
15-19	1.6587728431681699E-6
20-24	0.03748411932349272
25-29	0.0
30-34	8.293864215840849E-7
35-39	0.0
40-44	0.0
45-49	8.293864215840849E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	2.4881595536626596E-6
75-79	7.671829108206845E-4
80-84	0.07189881009455677
85-89	1.6587791992206923E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.294588893880572E-7
130-134	8.294925271723295E-7
135-139	1.6591335642836194E-6
140-144	4.0920302415477314E-4
145-149	0.04067184998230078
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	1.0
68-69	0.0
70-71	1.0
72-73	1.0
74-75	4.0
76-77	11.0
78-79	10.0
80-81	13.0
82-83	21.0
84-85	16.0
86-87	23.0
88-89	37.0
90-91	23.0
92-93	34.0
94-95	28.0
96-97	52.0
98-99	63.0
100-101	58.0
102-103	77.0
104-105	77.0
106-107	80.0
108-109	101.0
110-111	72.0
112-113	119.0
114-115	121.0
116-117	143.0
118-119	148.0
120-121	168.0
122-123	201.0
124-125	254.0
126-127	285.0
128-129	362.0
130-131	437.0
132-133	596.0
134-135	739.0
136-137	1512.0
138-139	4048.0
140-141	8353.0
142-143	9582.0
144-145	61458.0
146-147	795583.0
148-149	1049462.0
150-151	2.2179837E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.191507404492356
#Duplication Level	Percentage of total
1	25.92132705251479
2	8.22868336542437
3	5.146750511725654
4	3.58106458425876
5	2.6889581100618143
6	2.1368828998732123
7	1.8035774885457827
8	1.4593005317449064
9	1.3427221032972538
>10	16.86273880622485
>50	6.118599177595618
>100	13.240484016972099
>500	4.237014278851254
>1k	5.709760954343391
>5k	0.9370343472013989
>10k+	0.5851017713645313
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	31693	0.13142871929632202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.234607044177931E-4	2.0734660539602123E-5	0.0	0.0	0.0021937270850899047	0.0
2	0.0016463320468444087	3.31754568633634E-5	4.146932107920425E-6	0.0	0.007854289412401284	0.0
3	0.0026664773453928333	3.31754568633634E-5	4.146932107920425E-6	4.146932107920425E-6	0.010898137579614876	0.0
4	0.006552152730514271	3.31754568633634E-5	4.146932107920425E-6	8.29386421584085E-6	0.015683697232155045	0.0
5	0.006784380928557814	3.31754568633634E-5	4.146932107920425E-6	8.29386421584085E-6	0.01884365949839041	0.0
6	0.00790819952980425	3.7322388971283825E-5	4.146932107920425E-6	1.2440796323761273E-5	0.025051616863947284	0.0
7	0.010255363102887211	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.03229630725648427	0.0
8	0.017512494291747952	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.04388283556601393	0.0
9	0.0181511218363677	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.05277385800539532	0.0
10-11	0.01877523511860972	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.08973753734934403	0.0
12-13	0.0195113155677656	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.10540049992095948	0.0
14-15	0.02274799607799749	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.11319465881779592	0.0
16-17	0.026341312749510536	4.1469321079204245E-5	4.146932107920425E-6	1.2440796323761273E-5	0.11933211833751814	0.0
18-19	0.03385555372906235	4.354278713316446E-5	4.146932107920425E-6	1.2440796323761273E-5	0.12533065563162504	0.0
20-21	0.04025841690369149	5.391011740296552E-5	4.146932107920425E-6	1.2440796323761273E-5	0.12949002853586922	4.146932107920425E-6
22-23	0.04265327019601553	6.842437978068701E-5	4.146932107920425E-6	1.2440796323761273E-5	0.13398530294085498	4.146932107920425E-6
24-25	0.048288950930679385	7.257131188860744E-5	4.146932107920425E-6	1.2440796323761273E-5	0.13800990055159174	4.146932107920425E-6
26-27	0.053155375759324004	7.879171005048806E-5	4.146932107920425E-6	1.2440796323761273E-5	0.14132537277187412	4.146932107920425E-6
28-29	0.06492229561554821	8.293864215840849E-5	4.146932107920425E-6	1.2440796323761273E-5	0.14412662541077437	4.146932107920425E-6
30-31	0.08818036434281992	8.50121082123687E-5	4.146932107920425E-6	1.2440796323761273E-5	0.14702325748815678	4.146932107920425E-6
32-33	0.10769582684269342	9.123250637424935E-5	4.146932107920425E-6	1.2440796323761273E-5	0.14928333548697342	4.146932107920425E-6
34-35	0.12194261209945405	9.330597242820956E-5	4.146932107920425E-6	1.2440796323761273E-5	0.15170929077010686	4.146932107920425E-6
36-37	0.12435197965415581	9.537943848216977E-5	4.146932107920425E-6	1.2440796323761273E-5	0.15331000656376415	4.146932107920425E-6
38-39	0.12652082514659818	9.745290453612999E-5	4.146932107920425E-6	1.2440796323761273E-5	0.154620437109867	4.146932107920425E-6
40-41	0.12787687194588818	1.0574676875197083E-4	4.146932107920425E-6	1.2440796323761273E-5	0.15599514510364262	4.146932107920425E-6
42-43	0.1281630102613347	1.161140990217719E-4	4.146932107920425E-6	1.2440796323761273E-5	0.15753158344962714	4.146932107920425E-6
44-45	0.1289695885563252	1.2233449718365252E-4	4.146932107920425E-6	1.2440796323761273E-5	0.15866162244903545	4.146932107920425E-6
46-47	0.13029660683085975	1.3892222561533423E-4	4.146932107920425E-6	1.2440796323761273E-5	0.1599720529951383	8.29386421584085E-6
48-49	0.13143701316053785	1.4099569166929444E-4	4.146932107920425E-6	1.2440796323761273E-5	0.16099427175974068	8.29386421584085E-6
50-51	0.13241361567195314	1.4099569166929444E-4	4.146932107920425E-6	1.4514262377721485E-5	0.1619418457464005	1.866119448564191E-5
52-53	0.13354780160346935	1.513630219390955E-4	4.146932107920425E-6	1.65877284316817E-5	0.16294955024862517	2.0734660539602123E-5
54-55	0.13414910675911781	1.5550995404701594E-4	4.146932107920425E-6	1.866119448564191E-5	0.16391371196371668	2.0734660539602123E-5
56-57	0.1350925338136697	1.5965688615493634E-4	4.146932107920425E-6	2.0734660539602123E-5	0.1647037025302755	2.0734660539602123E-5
58-59	0.1352708518943103	1.6173035220889655E-4	4.146932107920425E-6	2.0734660539602123E-5	0.16548954616472644	2.0734660539602123E-5
60-61	0.1356399288519152	1.6173035220889655E-4	4.146932107920425E-6	2.0734660539602123E-5	0.1662048919533427	2.0734660539602123E-5
62-63	0.13593021409946965	1.6173035220889655E-4	4.146932107920425E-6	2.0734660539602123E-5	0.16701976411254904	2.0734660539602123E-5
64-65	0.13598619768292658	1.700242164247374E-4	4.146932107920425E-6	2.695505870148276E-5	0.16780975467910791	2.0734660539602123E-5
66-67	0.1360940179177325	1.7831808064057826E-4	4.146932107920425E-6	2.9028524755442974E-5	0.1684131333008103	2.0734660539602123E-5
68-69	0.13614585456908151	1.824650127484987E-4	4.146932107920425E-6	2.9028524755442974E-5	0.16909530363256325	2.2808126593562336E-5
70-71	0.13627233599837307	1.8661194485641912E-4	4.146932107920425E-6	2.9028524755442974E-5	0.1698479718101508	2.4881592647522547E-5
72-73	0.13631587878550624	1.9905274118018037E-4	4.146932107920425E-6	2.9028524755442974E-5	0.1703684117896948	2.4881592647522547E-5
74-75	0.13633661344604586	2.0112620723414059E-4	4.146932107920425E-6	2.9028524755442974E-5	0.1709738638774512	2.4881592647522547E-5
76-77	0.13636771543685525	2.05273139342061E-4	4.146932107920425E-6	2.9028524755442974E-5	0.17173897285136253	2.4881592647522547E-5
78-79	0.1364444336808518	2.0734660539602123E-4	4.146932107920425E-6	2.9028524755442974E-5	0.172806807869152	2.4881592647522547E-5
80-81	0.1364962703322008	2.0942007144998144E-4	4.146932107920425E-6	2.9028524755442974E-5	0.17365900241732968	2.4881592647522547E-5
82-83	0.13652115192484832	2.1564046961186208E-4	4.146932107920425E-6	2.9028524755442974E-5	0.17447180111048205	2.4881592647522547E-5
84-85	0.13656469471198146	2.1564046961186208E-4	4.146932107920425E-6	2.9028524755442974E-5	0.17537168537790082	2.4881592647522547E-5
86-87	0.13660201710095277	2.1564046961186208E-4	4.146932107920425E-6	3.524892291732361E-5	0.17619692486737695	2.4881592647522547E-5
88-89	0.13662482522754632	2.197874017197825E-4	4.146932107920425E-6	4.1469321079204245E-5	0.17701179702658332	2.4881592647522547E-5
90-91	0.13664348642203195	2.2393433382770294E-4	4.146932107920425E-6	4.1469321079204245E-5	0.1775446778024511	2.4881592647522547E-5
92-93	0.13665385375230177	2.2393433382770294E-4	8.29386421584085E-6	4.1469321079204245E-5	0.17814390949204562	2.4881592647522547E-5
94-95	0.13669532307338095	2.2600779988166315E-4	8.29386421584085E-6	4.1469321079204245E-5	0.17881571249352873	2.4881592647522547E-5
96-97	0.13669947000548888	2.3637513015146422E-4	8.29386421584085E-6	4.1469321079204245E-5	0.17952691135003707	2.4881592647522547E-5
98-99	0.13671191080181264	2.3637513015146422E-4	1.0367330269801061E-5	4.1469321079204245E-5	0.18010333491303798	2.4881592647522547E-5
100-101	0.13674923319078391	2.3844859620542443E-4	1.2440796323761273E-5	4.1469321079204245E-5	0.18062170142652806	2.695505870148276E-5
102-103	0.13677411478343143	2.4466899436730504E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18112762714369435	2.9028524755442974E-5
104-105	0.1367824086476473	2.4466899436730504E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18174966695988243	2.9028524755442974E-5
106-107	0.13681143717240274	2.529628585831459E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18229291506601997	2.9028524755442974E-5
108-109	0.13684875956137402	2.5918325674502657E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18285275090058925	2.9028524755442974E-5
110-111	0.13688400848429133	2.7369751912274804E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18348930497915503	2.9028524755442974E-5
112-113	0.13690059621272302	2.7784445123066846E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18418391610723173	2.9028524755442974E-5
114-115	0.13691096354299281	2.7784445123066846E-4	1.65877284316817E-5	4.1469321079204245E-5	0.184847425244499	2.9028524755442974E-5
116-117	0.13691511047510074	2.7784445123066846E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18537615908825883	2.9028524755442974E-5
118-119	0.13691925740720867	2.819913833385889E-4	1.65877284316817E-5	4.1469321079204245E-5	0.1859090398641266	2.9028524755442974E-5
120-121	0.13691925740720867	2.819913833385889E-4	1.65877284316817E-5	4.1469321079204245E-5	0.18654144701058445	3.31754568633634E-5
122-123	0.1369275512714245	2.819913833385889E-4	1.65877284316817E-5	4.1469321079204245E-5	0.1870660339222364	4.1469321079204245E-5
124-125	0.13695657979617992	2.819913833385889E-4	2.0734660539602123E-5	4.1469321079204245E-5	0.18761135549442792	4.7689719241084886E-5
126-127	0.1369897552530433	2.819913833385889E-4	2.0734660539602123E-5	4.354278713316446E-5	0.1883121870206665	4.976318529504509E-5
128-129	0.13701256337963685	2.9235871360839E-4	2.0734660539602123E-5	4.561625318712467E-5	0.18906900213036198	5.5983583456925734E-5
130-131	0.1370208572438527	2.9443217966235017E-4	2.0734660539602123E-5	4.561625318712467E-5	0.1898859477556223	6.0130515564846154E-5
132-133	0.13703329804017647	2.9443217966235017E-4	2.0734660539602123E-5	4.561625318712467E-5	0.19054116302867372	6.220398161880637E-5
134-135	0.1370415919043923	2.9443217966235017E-4	2.0734660539602123E-5	4.561625318712467E-5	0.1911258804558905	6.63509137267268E-5
136-137	0.137058179632824	2.9443217966235017E-4	2.0734660539602123E-5	4.561625318712467E-5	0.19167949589229788	7.049784583464722E-5
138-139	0.13706025309887795	2.985791117702706E-4	2.0734660539602123E-5	4.561625318712467E-5	0.1923388580974572	7.257131188860744E-5
>>END_MODULE
