##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.2.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24097071
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	76-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.50015642980012	36.0	34.0	38.0	31.0	39.0
2	34.8325704812838	35.0	34.0	38.0	31.0	39.0
3	34.933669075382646	35.0	34.0	38.0	31.0	39.0
4	34.962955539285254	36.0	34.0	38.0	31.0	39.0
5	34.83110246054385	35.0	34.0	37.0	31.0	39.0
6	34.812318310387184	35.0	34.0	37.0	32.0	39.0
7	34.83260737373434	35.0	34.0	37.0	32.0	39.0
8	34.97105005002475	35.0	34.0	37.0	32.0	39.0
9	34.93636272225782	35.0	34.0	37.0	32.0	39.0
10-14	34.91858963274002	35.2	34.0	37.4	31.6	39.0
15-19	34.933676578369216	35.0	34.0	37.0	31.6	39.0
20-24	34.92131998117115	35.0	34.0	37.0	31.2	39.0
25-29	34.92313699868337	35.0	34.0	37.0	31.4	39.0
30-34	34.90296869690097	35.0	34.0	37.0	31.0	39.0
35-39	34.893712542906144	35.0	34.0	37.0	31.0	39.0
40-44	34.850719002321895	35.0	34.0	37.0	31.0	39.0
45-49	34.74876393068685	35.0	34.0	37.0	31.0	39.0
50-54	34.7255245917647	35.0	34.0	37.0	31.0	39.0
55-59	34.71744834880555	35.0	34.0	37.0	31.0	39.0
60-64	34.688734161923655	35.0	34.0	37.0	31.0	39.0
65-69	34.654260146388744	35.0	34.0	37.0	31.0	39.0
70-74	34.658338575671706	35.0	34.0	37.0	31.0	39.0
75-79	34.64268516150042	35.0	34.0	37.0	31.0	39.0
80-84	34.6234921412089	35.0	34.0	37.0	31.0	39.0
85-89	34.61128160750768	35.0	34.0	37.0	30.6	39.0
90-94	34.5849524209704	35.0	34.0	37.0	30.0	39.0
95-99	34.56633994202334	35.0	34.0	37.0	30.0	39.0
100-104	34.5279195153098	35.0	34.0	37.0	30.0	39.0
105-109	34.499505748059406	35.0	34.0	37.0	30.0	39.0
110-114	34.46837150995086	35.0	34.0	37.0	30.0	39.0
115-119	34.433426508299945	35.0	34.0	37.0	30.0	39.0
120-124	34.391244342383274	35.0	34.0	37.0	30.0	39.0
125-129	34.37328105457124	35.0	34.0	37.0	30.0	39.0
130-134	34.32768217089169	35.0	34.0	37.0	30.0	39.0
135-139	34.28674253386801	35.0	34.0	37.0	30.0	39.0
140-144	34.245353071543434	35.0	34.0	37.0	29.4	39.0
145-149	34.27825273628057	35.0	34.0	37.0	29.8	39.0
150	35.13355722409546	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	6.0
22	196.0
23	2454.0
24	17792.0
25	72036.0
26	188502.0
27	351545.0
28	530894.0
29	698720.0
30	880616.0
31	1110040.0
32	1420107.0
33	1877468.0
34	2594403.0
35	4055391.0
36	8730947.0
37	1565945.0
38	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.92838195978258	11.194551404193481	15.246604037478248	38.63046259854569
2	23.1994240524103	12.934352623563198	36.04166423721285	27.82455908681365
3	21.386582652261733	17.63101755482055	28.107579879152954	32.87481991376476
4	26.56051784598189	25.246356929431485	21.885078127201385	26.308047097385245
5	26.62049256141497	30.71319583603906	23.833508956446796	18.832802646099175
6	19.68241857182269	36.275946314455574	25.32782831966583	18.713806794055905
7	16.746336825059288	24.334324422384952	39.696533946608675	19.22280480594708
8	19.104679581304747	23.90009249680324	31.108585121771547	25.886642800120462
9	20.11567639062239	22.82853935825843	32.12597955965734	24.929804691461843
10-14	21.045670468678278	28.655601470474796	26.519035546666387	23.779692514180542
15-19	21.283920853635028	27.60044426432801	27.204148551967798	23.91148633006916
20-24	21.23197426347483	27.69622384046765	27.093632406476786	23.97816948958074
25-29	21.18248698444481	27.875217794630547	26.811250230982985	24.131044989941657
30-34	21.18366583562052	27.66177217983643	27.012694334633252	24.1418676499098
35-39	21.45393251471942	27.4510239252857	26.913370369193956	24.181673190800925
40-44	21.605881593510762	27.533644323546074	26.769522870620406	24.090951212322757
45-49	21.809599666335632	27.462066568770226	26.68331746056066	24.045016304333483
50-54	21.812281057188503	27.6416462001193	26.627327681575576	23.91874506111662
55-59	21.721609968017972	27.601970718787843	26.690794724821583	23.9856245883726
60-64	21.919637069899306	27.44312204703703	26.483893294416443	24.153347588647225
65-69	21.800413887830057	27.53679204559088	26.656802457980334	24.00599160859873
70-74	22.136856053520752	27.317422332274326	26.59580559592452	23.949916018280394
75-79	21.998218187513036	27.195176807677285	26.605886079882307	24.200718924927372
80-84	22.25015271793859	27.19890778942285	26.595631159351356	23.9553083332872
85-89	22.114961454148514	27.20372846164853	26.606632954055392	24.074677130147567
90-94	22.100220074299187	26.982234865812575	26.595197547135502	24.32234751275273
95-99	22.31926908909816	26.984252351080347	26.771422304819914	23.925056255001582
100-104	22.280025258485438	26.82411662633637	26.808375929700194	24.08748218547799
105-109	22.359199379412168	26.75479442621318	26.74253711432783	24.143469080046827
110-114	22.321349944049082	26.797399248052738	26.746294682444287	24.13495612545389
115-119	22.427751788541237	26.958180650908886	26.647673509662955	23.966394050886926
120-124	22.353186264797834	26.816225906556056	26.67575857876596	24.15482924988015
125-129	22.691072448376314	26.78326659983744	26.576615984092992	23.94904496769325
130-134	22.45183123374094	27.00357973947688	26.491317195068177	24.053271831714003
135-139	22.54795814776868	26.829702002416926	26.541896296428373	24.080443553386022
140-144	22.53633889030678	26.68302173866543	26.772647914078973	24.00799145694881
145-149	22.008339199937467	26.814808097009262	27.037484269301853	24.13936843375142
150	21.809748402546795	25.395910428475872	27.72980611403955	25.06453505493778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.0
10	3.0
11	8.5
12	14.5
13	17.5
14	25.0
15	57.5
16	105.0
17	222.5
18	524.5
19	955.0
20	1555.5
21	2536.0
22	4227.0
23	6911.5
24	11199.5
25	17235.5
26	24941.0
27	36418.5
28	55914.5
29	82467.0
30	111482.5
31	140700.0
32	171355.0
33	206060.0
34	241288.0
35	287336.0
36	355470.0
37	426530.5
38	499154.0
39	604406.5
40	746043.5
41	905638.0
42	1070954.5
43	1231285.0
44	1371792.5
45	1477489.0
46	1548780.5
47	1565684.0
48	1517098.5
49	1462125.0
50	1375633.0
51	1224818.5
52	1036663.0
53	867156.5
54	723682.5
55	596158.5
56	501546.0
57	409976.0
58	335454.5
59	272663.5
60	206708.0
61	143471.0
62	85690.0
63	49416.0
64	31669.0
65	19861.5
66	13331.5
67	6689.5
68	3068.5
69	1886.5
70	1470.5
71	1350.5
72	1212.5
73	840.0
74	435.0
75	143.5
76	37.0
77	23.5
78	10.5
79	3.5
80	3.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018010487664662648
3	0.15383612390070145
4	9.129740290842817E-5
5	6.224822925574648E-5
6	2.904917365268169E-5
7	4.149881950383098E-5
8	9.129740290842817E-5
9	7.884775705727887E-5
10-14	1.5686553772448114E-4
15-19	2.7140227955505463E-4
20-24	3.3448048520087775E-4
25-29	2.415231295122963E-4
30-34	2.2326364893061068E-4
35-39	1.228365057313397E-4
40-44	2.655924448245183E-4
45-49	1.7346506552601352E-4
50-54	1.0374704875957747E-4
55-59	1.485657738237149E-4
60-64	1.8757466415731606E-4
65-69	0.06220922036541288
70-74	1.7097513635578367E-4
75-79	1.3943604047658942E-4
80-84	9.627729960467295E-5
85-89	8.880759756798983E-5
90-94	1.410964793324284E-4
95-99	8.382821096347008E-5
100-104	5.809903107664883E-5
105-109	1.0208882873486792E-4
110-114	5.477976162038933E-5
115-119	4.4820218261016866E-5
120-124	1.062416099216118E-4
125-129	1.0956324438679278E-4
130-134	4.316227440583622E-5
135-139	3.6523764091646396E-5
140-144	5.397363490899783E-5
145-149	5.837343489611652E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
76-77	2.0
78-79	2.0
80-81	7.0
82-83	3.0
84-85	14.0
86-87	11.0
88-89	20.0
90-91	25.0
92-93	28.0
94-95	38.0
96-97	43.0
98-99	45.0
100-101	47.0
102-103	46.0
104-105	51.0
106-107	54.0
108-109	61.0
110-111	77.0
112-113	96.0
114-115	96.0
116-117	79.0
118-119	90.0
120-121	112.0
122-123	128.0
124-125	138.0
126-127	164.0
128-129	210.0
130-131	254.0
132-133	315.0
134-135	428.0
136-137	813.0
138-139	2003.0
140-141	5215.0
142-143	7816.0
144-145	67575.0
146-147	767610.0
148-149	1341305.0
150-151	2.190205E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.372092489753726
#Duplication Level	Percentage of total
1	35.937057916567895
2	8.885228468723778
3	5.246785087644248
4	3.5129102820880083
5	2.619079717819019
6	2.044922010536915
7	1.690797799705841
8	1.4534119905346286
9	1.2266844370005086
>10	15.30185381962653
>50	5.088131876983041
>100	10.047919168459138
>500	3.3360429688009203
>1k	3.317897249639469
>5k	0.23691466818296186
>10k+	0.054362537687045805
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.149881950383098E-6	1.2449645851149295E-5	0.0	0.0	2.738922087252845E-4	1.6599527801532394E-5
2	4.149881950383098E-6	4.149881950383099E-5	0.0	8.299763900766197E-6	0.002494079052180242	2.0749409751915494E-5
3	8.299763900766197E-6	4.149881950383099E-5	0.0	1.6599527801532394E-5	0.004652017666379453	3.319905560306479E-5
4	6.224822925574647E-5	4.149881950383099E-5	0.0	1.6599527801532394E-5	0.0068514551000824955	4.149881950383099E-5
5	7.884775705727887E-5	4.5648701454214084E-5	0.0	1.6599527801532394E-5	0.00805077098374321	4.5648701454214084E-5
6	1.0374704875957747E-4	4.5648701454214084E-5	0.0	2.0749409751915494E-5	0.009689974354144535	4.5648701454214084E-5
7	1.2449645851149294E-4	5.394846535498028E-5	0.0	2.0749409751915494E-5	0.01135822689819854	4.5648701454214084E-5
8	2.489929170229859E-4	5.809834730536338E-5	0.0	2.0749409751915494E-5	0.015147069118898309	4.979858340459718E-5
9	3.278406740802648E-4	5.809834730536338E-5	0.0	2.0749409751915494E-5	0.01837567727629636	4.979858340459718E-5
10-11	3.797141984600535E-4	6.0173288280554926E-5	0.0	2.0749409751915494E-5	0.03264504636268864	8.922246193323661E-5
12-13	4.253628999142676E-4	6.432317023093802E-5	0.0	2.0749409751915494E-5	0.04201547980665368	1.0789693070996056E-4
14-15	5.083605389219296E-4	6.639811120612957E-5	0.0	2.2824350727107042E-5	0.04862624175361396	1.2449645851149294E-4
16-17	5.872082959792085E-4	6.847305218132113E-5	0.0	2.489929170229859E-5	0.05296909321468987	1.2864634046187606E-4
18-19	7.034049905899352E-4	7.469787510689577E-5	0.0	2.489929170229859E-5	0.05639482076473112	1.307212814370676E-4
20-21	0.0010416203695461578	9.959716680919436E-5	0.0	2.697423267749014E-5	0.05936613624120542	1.3279622241225915E-4
22-23	0.00135286151582489	9.959716680919436E-5	0.0	2.904917365268169E-5	0.06241007465181142	1.4109598631302536E-4
24-25	0.0014918825611627239	1.0374704875957747E-4	4.149881950383098E-6	2.904917365268169E-5	0.06525274378782384	1.473208092386E-4
26-27	0.0015686553772448113	1.0582198973476901E-4	4.149881950383098E-6	4.149881950383099E-5	0.06768664955172353	1.639203370401324E-4
28-29	0.0017201260684337944	1.1204681266034366E-4	4.149881950383098E-6	4.5648701454214084E-5	0.07050649433700884	1.7429504191609013E-4
30-31	0.002027217332762144	1.1204681266034366E-4	4.149881950383098E-6	6.224822925574647E-5	0.07362720556369692	1.7844492386647322E-4
32-33	0.0021994374337030425	1.1619669461072676E-4	4.149881950383098E-6	7.469787510689578E-5	0.07653627281091549	2.2616856629587888E-4
34-35	0.0023052594234378113	1.1619669461072676E-4	4.149881950383098E-6	9.959716680919436E-5	0.07931461877669696	2.3239338922145352E-4
36-37	0.002342608360991259	1.1827163558591831E-4	4.149881950383098E-6	1.2449645851149294E-4	0.08179624818302605	2.3654327117183661E-4
38-39	0.002361282829767983	1.224215175363014E-4	4.149881950383098E-6	1.4524586826340845E-4	0.08444594780834566	2.469179760477944E-4
40-41	0.0023903320034206646	1.2449645851149294E-4	4.149881950383098E-6	1.4524586826340845E-4	0.08731766611801078	2.593676218989436E-4
42-43	0.0024048565902470053	1.265713994866845E-4	4.149881950383098E-6	1.5354563216417465E-4	0.08996736574333039	2.655924448245183E-4
44-45	0.0024276809409741126	1.2864634046187606E-4	4.149881950383098E-6	1.5562057313936618E-4	0.09304242826856426	2.655924448245183E-4
46-47	0.002458805055601986	1.2864634046187606E-4	4.149881950383098E-6	1.5769551411455774E-4	0.09805756060560222	2.697423267749014E-4
48-49	0.0024795544653539014	1.3694610436264227E-4	4.149881950383098E-6	1.597704550897493E-4	0.10090645456454023	2.697423267749014E-4
50-51	0.002494079052180242	1.514706911889831E-4	4.149881950383098E-6	1.6184539606494083E-4	0.10404376531902985	2.863418545764338E-4
52-53	0.002523128225832924	1.5354563216417465E-4	4.149881950383098E-6	1.6184539606494083E-4	0.10654406919413567	2.904917365268169E-4
54-55	0.002556327281435989	1.5354563216417465E-4	4.149881950383098E-6	1.7014515996570704E-4	0.10833681819670117	2.946416184772E-4
56-57	0.0026351750384932676	1.5562057313936618E-4	4.149881950383098E-6	1.7636998289128166E-4	0.11052588092552826	2.946416184772E-4
58-59	0.0026891235038482477	1.597704550897493E-4	4.149881950383098E-6	1.8674468776723943E-4	0.11261534648754615	2.946416184772E-4
60-61	0.0028011703165085914	1.6184539606494083E-4	4.149881950383098E-6	1.9296951069281408E-4	0.11465086358420906	3.029413823779662E-4
62-63	0.0028634185457643378	1.7014515996570704E-4	4.149881950383098E-6	1.9504445166800563E-4	0.11722794027539696	3.029413823779662E-4
64-65	0.002886242896491445	1.7014515996570704E-4	4.149881950383098E-6	2.1371892044472955E-4	0.12009343376213649	3.029413823779662E-4
66-67	0.002898692542342594	1.7844492386647322E-4	4.149881950383098E-6	2.282435072710704E-4	0.12244226694605331	3.0501632335315775E-4
68-69	0.0029277417159952763	1.9504445166800563E-4	4.149881950383098E-6	2.282435072710704E-4	0.12432216346957686	3.1124114627873237E-4
70-71	0.0029692405354991066	2.0541915654396337E-4	4.149881950383098E-6	2.282435072710704E-4	0.12637220515306613	3.153910282291155E-4
72-73	0.002992064886226214	2.2409362532068732E-4	4.149881950383098E-6	2.282435072710704E-4	0.12812553027710297	3.153910282291155E-4
74-75	0.0030128142959781295	2.2616856629587885E-4	4.149881950383098E-6	2.3239338922145352E-4	0.12989960481089174	3.153910282291155E-4
76-77	0.0030314887647548538	2.3239338922145352E-4	4.149881950383098E-6	2.3446833019664508E-4	0.1314827847749629	3.153910282291155E-4
78-79	0.003046013351581194	2.4276809409741126E-4	4.149881950383098E-6	2.5521773994856056E-4	0.13306596473903404	3.153910282291155E-4
80-81	0.0030626128793827265	2.510678579981775E-4	4.149881950383098E-6	2.572926809237521E-4	0.13469271846358422	3.1746596920430705E-4
82-83	0.0030916620530354083	2.572926809237521E-4	4.149881950383098E-6	2.572926809237521E-4	0.13642114429591878	3.195409101794986E-4
84-85	0.003143535577415197	2.614425628741352E-4	4.149881950383098E-6	2.572926809237521E-4	0.13824501741311218	3.195409101794986E-4
86-87	0.003172584751067879	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.13976179926597718	3.195409101794986E-4
88-89	0.0031871093378942196	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.14136987852175065	3.195409101794986E-4
90-91	0.0031974840427701776	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.14350499278522272	3.195409101794986E-4
92-93	0.00321408357057171	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.1453309408433913	3.195409101794986E-4
94-95	0.003265957094951499	2.780420906756676E-4	4.149881950383098E-6	2.572926809237521E-4	0.14686224728308267	3.195409101794986E-4
96-97	0.003332355206157628	2.780420906756676E-4	4.149881950383098E-6	2.572926809237521E-4	0.14827113220523772	3.195409101794986E-4
98-99	0.003400828258338949	2.780420906756676E-4	4.149881950383098E-6	2.593676218989436E-4	0.14956382043278205	3.195409101794986E-4
100-101	0.0035149500119744845	2.821919726260507E-4	4.149881950383098E-6	2.6351750384932674E-4	0.150906307243731	3.2161585115469016E-4
102-103	0.0035232497758752508	2.821919726260507E-4	4.149881950383098E-6	2.655924448245183E-4	0.15205374960301193	3.2369079212988167E-4
104-105	0.0035460741266023577	2.863418545764338E-4	4.149881950383098E-6	2.780420906756676E-4	0.15312234420523557	3.2369079212988167E-4
106-107	0.0035917228280565715	2.9256667750200845E-4	4.149881950383098E-6	2.863418545764338E-4	0.15414114022405462	3.2369079212988167E-4
108-109	0.0036166221197588702	2.946416184772E-4	4.149881950383098E-6	2.904917365268169E-4	0.15531555681601306	3.257657331050732E-4
110-111	0.003653971057312318	2.946416184772E-4	4.149881950383098E-6	2.904917365268169E-4	0.15655637151917756	3.278406740802648E-4
112-113	0.0037162192865680646	2.946416184772E-4	4.149881950383098E-6	2.904917365268169E-4	0.15777436187161503	3.319905560306479E-4
114-115	0.003761867988022279	2.946416184772E-4	4.149881950383098E-6	2.9671655945239157E-4	0.15911062385963837	3.319905560306479E-4
116-117	0.003815816453377259	2.946416184772E-4	4.149881950383098E-6	3.070912643283493E-4	0.160092070940904	3.319905560306479E-4
118-119	0.003875989741657814	2.987915004275831E-4	4.149881950383098E-6	3.091662053035408E-4	0.16099674520608748	3.319905560306479E-4
120-121	0.003981811731392583	3.029413823779662E-4	4.149881950383098E-6	3.257657331050732E-4	0.16196574264150196	3.319905560306479E-4
122-123	0.0041664814781846304	3.029413823779662E-4	4.149881950383098E-6	3.4444020188179714E-4	0.1629637892505691	3.319905560306479E-4
124-125	0.004234954530365952	3.070912643283493E-4	4.149881950383098E-6	3.4859008383218026E-4	0.1639016625713557	3.319905560306479E-4
126-127	0.004268153585969017	3.070912643283493E-4	4.149881950383098E-6	3.527399657825634E-4	0.16475446331215937	3.319905560306479E-4
128-129	0.004317952169373614	3.091662053035408E-4	4.149881950383098E-6	3.527399657825634E-4	0.16547654277152607	3.319905560306479E-4
130-131	0.0044486734508106815	3.1124114627873237E-4	4.149881950383098E-6	3.527399657825634E-4	0.16624219599137174	3.3614043798103096E-4
132-133	0.004506771798116045	3.1124114627873237E-4	4.149881950383098E-6	3.631146706585211E-4	0.16696842533268877	3.3614043798103096E-4
134-135	0.0046022190829748556	3.1124114627873237E-4	4.149881950383098E-6	3.651896116337127E-4	0.16783367571934366	3.402903199314141E-4
136-137	0.004737090246362307	3.1331608725392393E-4	4.149881950383098E-6	3.651896116337127E-4	0.1686055537621149	3.465151428569887E-4
138-139	0.0047433150692878815	3.195409101794986E-4	4.149881950383098E-6	3.6933949358409574E-4	0.16937535686391098	3.4859008383218026E-4
>>END_MODULE
