##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24097071
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.012988674017684	37.0	35.0	38.0	33.0	39.0
2	35.34433566635547	35.0	34.0	37.0	33.0	39.0
3	35.4540445185226	35.0	34.0	37.0	33.0	39.0
4	35.53459887303316	36.0	35.0	38.0	33.0	39.0
5	35.45313523788845	36.0	35.0	38.0	33.0	39.0
6	35.39942680170549	35.0	35.0	37.0	33.0	39.0
7	35.44002401785678	35.0	34.0	38.0	33.0	39.0
8	35.564351119685874	36.0	35.0	38.0	33.0	39.0
9	35.560950083933434	36.0	35.0	38.0	33.0	39.0
10-14	35.51731897208586	35.8	34.8	37.8	33.0	39.0
15-19	35.488626480786806	36.0	35.0	38.0	33.0	39.0
20-24	35.46094941580244	36.0	34.8	38.0	33.0	39.0
25-29	35.452785012751136	36.0	34.0	38.0	33.0	39.0
30-34	35.43888064238181	36.0	34.0	38.0	33.0	39.0
35-39	35.410437426191756	36.0	34.0	38.0	33.0	39.0
40-44	35.39578041663238	36.0	34.0	38.0	33.0	39.0
45-49	35.36681478840312	36.0	34.0	38.0	32.6	39.0
50-54	35.34847549729177	36.0	34.0	38.0	32.0	39.0
55-59	35.31801824379403	36.0	34.0	38.0	32.0	39.0
60-64	35.27890949900093	36.0	34.0	38.0	32.0	39.0
65-69	35.24239651912306	36.0	34.0	38.0	32.0	39.0
70-74	35.22378258913748	36.0	34.0	38.0	32.0	39.0
75-79	35.168577359933636	36.0	34.0	38.0	32.0	39.0
80-84	35.09212135279321	36.0	34.0	38.0	32.0	39.0
85-89	35.07928519446895	35.8	34.0	38.0	32.0	39.0
90-94	35.03261555282462	35.2	34.0	38.0	32.0	39.0
95-99	34.96704961974201	35.2	34.0	37.6	31.0	39.0
100-104	34.90300294227043	35.0	34.0	37.4	31.0	39.0
105-109	34.850001744935604	35.0	34.0	37.2	31.0	39.0
110-114	34.77830602450183	35.0	34.0	37.0	31.0	39.0
115-119	34.7168174990597	35.0	34.0	37.0	30.4	39.0
120-124	34.647201177899206	35.0	34.0	37.0	30.0	39.0
125-129	34.56722613141946	35.0	34.0	37.0	30.0	39.0
130-134	34.491874714076594	35.0	34.0	37.0	30.0	39.0
135-139	34.409860429635025	35.0	34.0	37.0	30.0	39.0
140-144	34.31671121204614	35.0	34.0	37.0	29.0	39.0
145-149	34.28142297578898	35.0	34.0	37.0	29.2	39.0
150	35.08559549855378	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	13.0
21	152.0
22	1436.0
23	8201.0
24	28013.0
25	70567.0
26	143037.0
27	252357.0
28	378376.0
29	502013.0
30	643323.0
31	834493.0
32	1108583.0
33	1534738.0
34	2291141.0
35	3873607.0
36	1.0234124E7
37	2192889.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.4895473395916	18.439581308450308	15.220111191107003	32.85076016085108
2	24.707679154479674	25.021721523204466	32.86016509810094	17.41043422421492
3	19.872867536473624	28.956083500770696	29.97004490711755	21.20100405563813
4	23.729415081193896	33.76289176389944	21.750170383778176	20.757522771128492
5	25.69568724763271	34.84485728576722	21.70431003834449	17.755145428255574
6	19.168765365716023	38.81981341217777	22.095320215473492	19.916101006632715
7	20.53483595578898	20.706429424555374	36.93936495435482	21.819369665300815
8	21.493085196951945	23.36786491603067	28.265933233130287	26.873116653887102
9	23.563967587596018	23.419099358590096	27.8847582762237	25.132174777590187
10-14	23.18871451223263	28.85151311543216	25.229921097049512	22.729851275285696
15-19	23.2420962966171	27.727349334094164	26.701675292417697	22.328879076871036
20-24	23.131875672915104	27.664411714132026	26.670526676087626	22.53318593686524
25-29	23.21915721624425	27.25618561691585	26.686830943063576	22.837826223776325
30-34	23.04716535881062	27.01227215540013	26.91947581513123	23.021086670658022
35-39	23.360785217423313	26.800321914642655	26.92683272585286	22.912060142081167
40-44	23.527663590317676	26.469790457105763	26.973792790003397	23.02875316257316
45-49	23.420694573211822	26.29092058532757	27.07652311768513	23.211861723775478
50-54	23.614158945825068	26.30407426947511	26.816900085631985	23.264866699067838
55-59	23.64273566691985	26.31995813931079	26.848290400107132	23.189015793662225
60-64	23.353992690646923	26.28506012203724	26.924816713201366	23.43613047411447
65-69	23.549840267714544	26.23323045056068	26.91029534977149	23.306633931953286
70-74	23.73652902234161	26.21925480114108	26.62808719362947	23.41612898288784
75-79	23.551405226321656	26.32728660940878	26.89361851453869	23.227689649730877
80-84	23.843245639960355	26.327648933996876	26.58501084970367	23.24409457633909
85-89	23.589612976473926	26.385828753671248	26.814086668075642	23.210471601779183
90-94	23.61094848061879	26.136408945871885	26.84075962536707	23.411882948142253
95-99	23.589565636339717	26.35856974003464	26.87497999213322	23.176884631492424
100-104	23.836995723774056	26.13902244498214	26.818444307388546	23.205537523855256
105-109	23.62957082863449	26.321850013582974	26.75234689149083	23.296232266291707
110-114	23.71963766458805	26.4185120156515	26.638899280638594	23.22295103912185
115-119	23.84405615292588	26.4936958251346	26.7938655774211	22.868382444518417
120-124	23.800583805258757	26.61893206681858	26.915741892213624	22.664742235709042
125-129	23.80296982598923	26.676457818685336	26.75507562023484	22.765496735090597
130-134	23.95446910478248	26.688455681900397	26.76329103698035	22.593784176336772
135-139	23.715793938064337	26.722825073725947	27.0199612186175	22.54141976959222
140-144	23.754741300440315	26.850067833611003	26.811454458878302	22.58373640707038
145-149	23.526584248643882	27.138232776166188	26.641111119389187	22.694071855800747
150	23.708860347852166	25.9980515292379	26.909270999131003	23.38381712377893
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	3.0
2	9.0
3	21.5
4	35.5
5	36.5
6	26.5
7	19.0
8	16.0
9	9.5
10	5.5
11	7.5
12	10.0
13	18.0
14	24.0
15	24.5
16	49.5
17	104.0
18	180.0
19	307.5
20	471.5
21	683.0
22	1084.5
23	1788.5
24	2881.0
25	4637.0
26	7284.5
27	11426.5
28	17329.0
29	24804.5
30	35257.0
31	50042.5
32	71262.5
33	98091.5
34	133261.0
35	187308.5
36	264311.0
37	358165.5
38	476056.5
39	621861.0
40	793705.5
41	983035.5
42	1178244.5
43	1371510.0
44	1522024.0
45	1613971.5
46	1640975.5
47	1610584.5
48	1561295.5
49	1504426.0
50	1393231.0
51	1231871.0
52	1048856.0
53	870488.0
54	728864.5
55	617224.0
56	506314.5
57	401708.0
58	331030.5
59	270121.5
60	202413.0
61	136438.0
62	84331.5
63	51606.5
64	32097.0
65	16488.0
66	9497.5
67	5409.0
68	3166.5
69	2097.0
70	1284.5
71	1043.5
72	911.0
73	740.0
74	611.0
75	374.5
76	129.5
77	55.0
78	15.5
79	3.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.6599527801532394E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	8.299763900766196E-7
20-24	0.03180967512607653
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.299763900766196E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	3.3199065247118902E-6
75-79	6.448922278020293E-4
80-84	0.062179463525113526
85-89	1.6599594070206689E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.4555466025148866E-4
145-149	0.03449891971570818
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	0.0
68-69	3.0
70-71	3.0
72-73	3.0
74-75	9.0
76-77	4.0
78-79	15.0
80-81	9.0
82-83	13.0
84-85	22.0
86-87	29.0
88-89	40.0
90-91	26.0
92-93	42.0
94-95	44.0
96-97	65.0
98-99	79.0
100-101	86.0
102-103	89.0
104-105	85.0
106-107	116.0
108-109	128.0
110-111	120.0
112-113	133.0
114-115	122.0
116-117	160.0
118-119	188.0
120-121	199.0
122-123	260.0
124-125	254.0
126-127	325.0
128-129	443.0
130-131	477.0
132-133	663.0
134-135	882.0
136-137	1551.0
138-139	4323.0
140-141	8377.0
142-143	10151.0
144-145	62861.0
146-147	791282.0
148-149	1072949.0
150-151	2.214044E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.38590754511616
#Duplication Level	Percentage of total
1	25.702581174425347
2	8.60301574275095
3	5.41235789830401
4	3.798475556780108
5	2.8060578267481753
6	2.3549729240101067
7	1.8335749366253384
8	1.5891992651307116
9	1.3564100649471433
>10	16.945306438568654
>50	6.17183870637533
>100	12.595261934758605
>500	4.031414957790718
>1k	5.398675771359701
>5k	0.8376296494743505
>10k+	0.5632271519506851
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	27558	0.11436244678865742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.780420906756676E-4	0.0	0.0	0.0	0.0022409362532068732	0.0
2	0.0014981073840882985	0.0	0.0	0.0	0.00889319701967098	0.0
3	0.0023446833019664507	0.0	0.0	0.0	0.012706938532073048	0.0
4	0.0057475865012805915	0.0	0.0	0.0	0.018720117478178158	0.0
5	0.005909431897345532	0.0	0.0	0.0	0.022774552143702446	0.0
6	0.007029900023948969	0.0	0.0	0.0	0.02969240535499107	0.0
7	0.009092391353289368	0.0	0.0	0.0	0.040589995356697085	0.0
8	0.016346385002559024	4.149881950383098E-6	0.0	0.0	0.0553179263986067	0.0
9	0.016935668239513424	8.299763900766197E-6	0.0	0.0	0.06611591923350352	0.0
10-11	0.017663972521805658	1.2449645851149295E-5	0.0	0.0	0.10849451371081573	0.0
12-13	0.01852299808553496	1.2449645851149295E-5	0.0	0.0	0.12851769412141417	0.0
14-15	0.021764055888784162	1.4524586826340845E-5	0.0	0.0	0.13790680203415592	0.0
16-17	0.025318429779287284	1.6599527801532394E-5	0.0	0.0	0.14498027581858391	0.0
18-19	0.03224873263642706	2.2824350727107042E-5	0.0	0.0	0.15165743587675035	0.0
20-21	0.038180988884499695	2.697423267749014E-5	0.0	0.0	0.15617250743876715	0.0
22-23	0.040554721360118826	2.904917365268169E-5	0.0	0.0	0.16084112463294814	0.0
24-25	0.0463562563267544	4.357376047902253E-5	0.0	0.0	0.16520265056280076	0.0
26-27	0.051776002153954725	5.809834730536338E-5	0.0	0.0	0.16884624691523714	0.0
28-29	0.06470910925232365	5.809834730536338E-5	0.0	2.074940975191549E-6	0.17191300967657025	0.0
30-31	0.0900835624379411	6.224822925574647E-5	0.0	4.149881950383098E-6	0.17515614242079464	0.0
32-33	0.1108578714815589	6.639811120612957E-5	0.0	4.149881950383098E-6	0.17778509263636233	0.0
34-35	0.1258161209717148	6.847305218132113E-5	0.0	4.149881950383098E-6	0.18082280622404273	0.0
36-37	0.1282417269717137	7.054799315651268E-5	0.0	4.149881950383098E-6	0.182872847907532	0.0
38-39	0.13065903320781186	7.262293413170422E-5	0.0	4.149881950383098E-6	0.18437718011454587	0.0
40-41	0.13223598834895745	7.677281608208731E-5	4.149881950383098E-6	4.149881950383098E-6	0.1859126364361876	0.0
42-43	0.13257212878693847	8.299763900766196E-5	4.149881950383098E-6	4.149881950383098E-6	0.18766803650119968	0.0
44-45	0.1334705782291964	9.337234388361971E-5	4.149881950383098E-6	4.149881950383098E-6	0.1889648746106944	0.0
46-47	0.13481099009917014	9.959716680919436E-5	4.149881950383098E-6	4.149881950383098E-6	0.19037583447382464	0.0
48-49	0.135871284937493	9.959716680919436E-5	4.149881950383098E-6	4.149881950383098E-6	0.1915191269511552	0.0
50-51	0.13684650719583308	9.959716680919436E-5	4.149881950383098E-6	4.149881950383098E-6	0.19262922037288266	0.0
52-53	0.13783417910002424	1.0789693070996056E-4	6.224822925574648E-6	4.149881950383098E-6	0.1938409859023945	0.0
54-55	0.13834876446187172	1.141217536355352E-4	8.299763900766197E-6	4.149881950383098E-6	0.19494692944217162	0.0
56-57	0.1391185675636678	1.224215175363014E-4	8.299763900766197E-6	4.149881950383098E-6	0.1960030743985441	0.0
58-59	0.13927418813680717	1.2864634046187606E-4	8.299763900766197E-6	4.149881950383098E-6	0.19700734583053683	2.074940975191549E-6
60-61	0.1395605299913836	1.307212814370676E-4	8.299763900766197E-6	4.149881950383098E-6	0.1978041231650104	4.149881950383098E-6
62-63	0.13979084843962986	1.3279622241225915E-4	8.299763900766197E-6	4.149881950383098E-6	0.19876482083652408	4.149881950383098E-6
64-65	0.13983234725913368	1.390210453378338E-4	8.299763900766197E-6	4.149881950383098E-6	0.1997877667372935	4.149881950383098E-6
66-67	0.13991326995716616	1.6807021899051548E-4	8.299763900766197E-6	4.149881950383098E-6	0.2005783192488415	4.149881950383098E-6
68-69	0.13999211771422343	1.7844492386647322E-4	1.0374704875957745E-5	4.149881950383098E-6	0.20139999587501733	4.149881950383098E-6
70-71	0.14011868911371012	1.7844492386647322E-4	1.2449645851149295E-5	4.149881950383098E-6	0.20223412214704434	4.149881950383098E-6
72-73	0.14019131204784183	1.7844492386647322E-4	1.2449645851149295E-5	4.149881950383098E-6	0.20286697914447777	4.149881950383098E-6
74-75	0.14021206145759374	1.8051986484166478E-4	1.2449645851149295E-5	6.224822925574648E-6	0.20362640754139788	4.149881950383098E-6
76-77	0.14025563521807277	1.8259480581685634E-4	1.2449645851149295E-5	8.299763900766197E-6	0.20446675863635044	4.149881950383098E-6
78-79	0.14030958368342777	1.9089456971762252E-4	1.2449645851149295E-5	8.299763900766197E-6	0.20564947499220965	4.149881950383098E-6
80-81	0.14036560708975793	1.9504445166800563E-4	1.2449645851149295E-5	1.0374704875957745E-5	0.2066433717193264	8.299763900766197E-6
82-83	0.14040503096828655	1.971193926431972E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.2076165190366912	8.299763900766197E-6
84-85	0.14044860472876558	1.9919433361838872E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.20857306682625454	8.299763900766197E-6
86-87	0.14049840331217017	1.9919433361838872E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.2094134179212071	8.299763900766197E-6
88-89	0.14052745248582285	1.9919433361838872E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.21035336618296888	8.299763900766197E-6
90-91	0.14054612695459956	2.0334421556877181E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.21105262129160843	8.299763900766197E-6
92-93	0.1405668763643515	2.095690384943465E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.2115755064173567	1.0374704875957745E-5
94-95	0.14059800047897938	2.157938614199211E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.2122602369391699	1.2449645851149295E-5
96-97	0.14061252506580574	2.3239338922145352E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.21293459275610715	1.2449645851149295E-5
98-99	0.14062497471165686	2.3239338922145352E-4	2.0749409751915494E-5	1.2449645851149295E-5	0.2135446254028135	1.2449645851149295E-5
100-101	0.14066024870823512	2.3446833019664508E-4	2.0749409751915494E-5	1.2449645851149295E-5	0.21412975875781748	1.6599527801532394E-5
102-103	0.1406706234131111	2.3861821214702815E-4	2.4899291702298594E-5	1.2449645851149295E-5	0.21473149164062305	1.6599527801532394E-5
104-105	0.1407141971735901	2.4276809409741126E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2154307467492626	1.6599527801532394E-5
106-107	0.14073702152431722	2.489929170229859E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2160947278613239	1.6599527801532394E-5
108-109	0.1407598458750443	2.53142798973369E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21670268556705502	1.8674468776723945E-5
110-111	0.14080134469454814	2.614425628741352E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21731064327278615	2.0749409751915494E-5
112-113	0.14082624398625043	2.655924448245183E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21797047450289705	2.0749409751915494E-5
114-115	0.140842843514052	2.655924448245183E-4	2.904917365268169E-5	1.2449645851149295E-5	0.218619931028132	2.0749409751915494E-5
116-117	0.14085944304185352	2.655924448245183E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21910339227535164	2.0749409751915494E-5
118-119	0.1408677428057543	2.6766738579970986E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21976114856448736	2.0749409751915494E-5
120-121	0.14087189268770467	2.738922087252845E-4	2.904917365268169E-5	1.2449645851149295E-5	0.22040230532582156	2.489929170229859E-5
122-123	0.140886417274531	2.8011703165085915E-4	2.904917365268169E-5	1.2449645851149295E-5	0.22099366350375116	2.489929170229859E-5
124-125	0.14090094186135735	2.863418545764338E-4	2.904917365268169E-5	1.2449645851149295E-5	0.22159954626850709	2.489929170229859E-5
126-127	0.1409133915072085	2.9256667750200845E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22224070302984125	2.904917365268169E-5
128-129	0.14092169127110926	3.029413823779662E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22292750849262966	4.5648701454214084E-5
130-131	0.14092584115305964	3.091662053035408E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22376163476465666	5.187352437978873E-5
132-133	0.14094244068086118	3.1331608725392393E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22445673999134583	5.809834730536338E-5
134-135	0.1409631900906131	3.236907921298817E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22509789675268005	5.809834730536338E-5
136-137	0.14097148985451385	3.319905560306479E-4	3.7348937553447884E-5	1.6599527801532394E-5	0.22568510504865924	5.809834730536338E-5
138-139	0.14097148985451385	3.319905560306479E-4	3.7348937553447884E-5	1.6599527801532394E-5	0.22643415874070338	6.0173288280554926E-5
>>END_MODULE
