##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24129992
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.976852872557934	37.0	35.0	38.0	33.0	39.0
2	35.34259107918478	35.0	34.0	37.0	33.0	39.0
3	35.459594391908624	35.0	34.0	37.0	33.0	39.0
4	35.521593417851115	36.0	35.0	38.0	33.0	39.0
5	35.44703326051662	36.0	35.0	37.0	33.0	39.0
6	35.39296233500616	35.0	35.0	37.0	33.0	39.0
7	35.430278509831254	35.0	34.0	38.0	33.0	39.0
8	35.54935351822744	36.0	35.0	38.0	33.0	39.0
9	35.545883065357	36.0	35.0	38.0	33.0	39.0
10-14	35.504741526644516	35.8	34.6	37.8	33.0	39.0
15-19	35.478057100060376	36.0	34.6	38.0	33.0	39.0
20-24	35.45173186132842	36.0	34.4	38.0	33.0	39.0
25-29	35.44476445744367	36.0	34.0	38.0	33.0	39.0
30-34	35.4291127075384	36.0	34.0	38.0	33.0	39.0
35-39	35.40061349377986	36.0	34.0	38.0	33.0	39.0
40-44	35.38539625707294	36.0	34.0	38.0	32.8	39.0
45-49	35.35701154811821	36.0	34.0	38.0	32.0	39.0
50-54	35.33860186940799	36.0	34.0	38.0	32.0	39.0
55-59	35.307639745591295	36.0	34.0	38.0	32.0	39.0
60-64	35.26662360687618	36.0	34.0	38.0	32.0	39.0
65-69	35.23115231049141	36.0	34.0	38.0	32.0	39.0
70-74	35.212522987977074	36.0	34.0	38.0	32.0	39.0
75-79	35.15686815675567	36.0	34.0	38.0	32.0	39.0
80-84	35.08098995835329	36.0	34.0	38.0	32.0	39.0
85-89	35.065065997428334	35.8	34.0	38.0	32.0	39.0
90-94	35.01930510123664	35.2	34.0	38.0	31.6	39.0
95-99	34.95351115412863	35.2	34.0	37.4	31.0	39.0
100-104	34.888505517176846	35.0	34.0	37.4	31.0	39.0
105-109	34.83296705666014	35.0	34.0	37.0	31.0	39.0
110-114	34.762180549641656	35.0	34.0	37.0	31.0	39.0
115-119	34.698490286281455	35.0	34.0	37.0	30.0	39.0
120-124	34.625078736508065	35.0	34.0	37.0	30.0	39.0
125-129	34.54567424624419	35.0	34.0	37.0	30.0	39.0
130-134	34.46668868253371	35.0	34.0	37.0	30.0	39.0
135-139	34.37905227783888	35.0	34.0	37.0	29.8	39.0
140-144	34.28599213809741	35.0	34.0	37.0	29.0	39.0
145-149	34.24667081559958	35.0	34.0	37.0	29.0	39.0
150	35.05048135491626	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	144.0
22	1602.0
23	8474.0
24	29441.0
25	73793.0
26	147431.0
27	260404.0
28	383244.0
29	505585.0
30	648201.0
31	838910.0
32	1117574.0
33	1547735.0
34	2298672.0
35	3892089.0
36	1.0197237E7
37	2179441.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.23843240395604	19.486885863866014	15.472325892192588	30.802355839985356
2	24.338123458065112	26.30814076971682	31.64429692493462	17.709438847283447
3	19.900773278333453	29.969263976548355	29.057398775764202	21.07256396935399
4	23.059357002687776	34.59845738863071	21.610380144344845	20.731805464336663
5	24.98625362163402	35.30265571575821	21.863020095489464	17.8480705671183
6	19.047142659641164	39.13293050407974	22.12374956444246	19.696177271836643
7	20.579596545245433	20.938291235239532	36.84864462449884	21.633467595016196
8	21.6892612314169	23.411723468453697	28.278753677166574	26.620261622962825
9	23.663451691156798	23.27755433984396	27.939976109399456	25.11901785959979
10-14	23.151019693665877	28.607436753398012	25.39901380821013	22.84252974472598
15-19	23.12271218324482	27.53267883387612	26.873694777851565	22.470914205027505
20-24	22.979012064870254	27.58447530842037	26.77609273667746	22.66041989003192
25-29	23.093627217116357	27.226591703801645	26.730618062368194	22.949163016713804
30-34	22.941589868740945	26.9794718539484	26.922654595161077	23.156283682149585
35-39	23.2423118913591	26.71926870095937	26.97563347720961	23.06278593047192
40-44	23.386262208458252	26.399438507895074	27.013035064412787	23.201264219233888
45-49	23.311502962785895	26.234853289632255	27.115699831147893	23.337943916433954
50-54	23.480620639109404	26.261279801233904	26.814170190234798	23.44392936942189
55-59	23.53638907132667	26.246036882233533	26.866831949219044	23.350742097220753
60-64	23.212957106872757	26.221322016539784	26.959085224049563	23.606635652537896
65-69	23.428823439803967	26.15645819864444	26.94129647843786	23.473421883113737
70-74	23.584020517147167	26.140297557780233	26.64803269810922	23.62764922696338
75-79	23.398474353722108	26.276482519338508	26.924270371688824	23.400772755250564
80-84	23.71371972771477	26.233572818776135	26.624382661146566	23.428324792362524
85-89	23.472591812664596	26.317632440081674	26.843778821651647	23.36599692560208
90-94	23.479771253517956	26.07398910142948	26.884528944950574	23.56171070010199
95-99	23.431324563901786	26.27815575625806	26.90239264338573	23.38812703645442
100-104	23.70289462209027	26.07007916039144	26.82038327674721	23.406642940771075
105-109	23.50822032840931	26.23737514582868	26.769909837511275	23.48449468825073
110-114	23.594847224852312	26.335617859377937	26.65124371348909	23.41829120228066
115-119	23.73491870610894	26.409703114529638	26.815079050487505	23.040299128873922
120-124	23.7002032419491	26.550629490028747	26.944002475067197	22.805164792954958
125-129	23.650651714337517	26.615963161423785	26.787612892884944	22.94577223135375
130-134	23.82042938301972	26.63630486950426	26.78081444566875	22.762451301807268
135-139	23.610391037450515	26.630770664788944	27.069569217716754	22.689269080043783
140-144	23.614816637627616	26.788160070980858	26.846604006425846	22.750419284965677
145-149	23.44304527825884	27.168375693796794	26.56413783363635	22.82444119430801
150	23.562771127461446	26.014406569662974	26.858119346409893	23.56470295646569
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	11.5
3	25.0
4	40.5
5	40.5
6	28.5
7	23.0
8	13.5
9	8.5
10	8.5
11	7.0
12	8.0
13	13.0
14	17.0
15	26.0
16	60.5
17	113.0
18	180.5
19	276.5
20	432.0
21	671.0
22	1060.0
23	1775.5
24	2910.0
25	4667.0
26	7218.5
27	11112.0
28	16847.5
29	24281.5
30	34764.0
31	50196.0
32	72110.5
33	99943.0
34	136198.5
35	191745.5
36	270673.0
37	366136.5
38	485375.0
39	629095.0
40	797644.0
41	983254.5
42	1175159.5
43	1368989.0
44	1519880.0
45	1610372.5
46	1635959.0
47	1601028.5
48	1551608.0
49	1499738.5
50	1391030.5
51	1231712.5
52	1049073.0
53	872755.5
54	732862.0
55	619465.0
56	507222.5
57	404382.0
58	335215.0
59	274106.0
60	207001.5
61	140580.0
62	86682.5
63	52437.0
64	32593.5
65	16749.0
66	9306.5
67	5264.5
68	3255.0
69	2073.0
70	1085.5
71	910.5
72	804.0
73	652.5
74	557.5
75	349.5
76	112.0
77	43.5
78	14.0
79	2.5
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.144220188717841E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03078492524987161
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	4.97306422646141E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	6.241202846386343E-4
80-84	0.05953270531271914
85-89	1.6576969238681802E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.289127554238704E-7
125-129	0.0
130-134	1.6579518912431308E-6
135-139	8.290605762063859E-7
140-144	3.268443096459611E-4
145-149	0.031604662614500345
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	0.0
66-67	1.0
68-69	3.0
70-71	5.0
72-73	3.0
74-75	9.0
76-77	14.0
78-79	9.0
80-81	16.0
82-83	22.0
84-85	28.0
86-87	39.0
88-89	30.0
90-91	40.0
92-93	53.0
94-95	60.0
96-97	69.0
98-99	72.0
100-101	100.0
102-103	105.0
104-105	91.0
106-107	98.0
108-109	116.0
110-111	120.0
112-113	134.0
114-115	140.0
116-117	187.0
118-119	194.0
120-121	237.0
122-123	250.0
124-125	299.0
126-127	327.0
128-129	407.0
130-131	522.0
132-133	678.0
134-135	905.0
136-137	1611.0
138-139	4493.0
140-141	8782.0
142-143	10286.0
144-145	63249.0
146-147	791020.0
148-149	1089997.0
150-151	2.215517E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.13098269170227
#Duplication Level	Percentage of total
1	25.477104268830658
2	8.556353717654423
3	5.349116665050537
4	3.7529754946909377
5	2.8935238823829077
6	2.3251615926350184
7	1.8464348162268158
8	1.5762685527244986
9	1.3763760875051552
>10	17.167325506263765
>50	5.93665068385568
>100	12.718012968596451
>500	4.007085557032813
>1k	5.413628241986678
>5k	0.9602963807446596
>10k+	0.6436855838189657
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	29511	0.12230008198925221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.983838535876846E-4	0.0	4.144220188717841E-6	0.0	0.002631579819835829	0.0
2	0.001562371011146626	0.0	4.144220188717841E-6	0.0	0.010468300196701266	0.0
3	0.002349772847003016	0.0	4.144220188717841E-6	0.0	0.014239540568434503	0.0
4	0.005520101291372165	0.0	4.144220188717841E-6	0.0	0.019975141309619995	0.0
5	0.005685870098920878	4.144220188717841E-6	4.144220188717841E-6	0.0	0.02401575599361989	0.0
6	0.006560300558740343	8.288440377435682E-6	4.144220188717841E-6	0.0	0.0319850914165243	0.0
7	0.00835060368026645	8.288440377435682E-6	4.144220188717841E-6	0.0	0.04251969913624505	0.0
8	0.014189809926169888	8.288440377435682E-6	4.144220188717841E-6	0.0	0.05794863089884157	0.0
9	0.014720270110325772	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.06839620999459925	0.0
10-11	0.015399922221275498	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.11513472528295907	0.0
12-13	0.016249487359962657	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.13724413998976875	0.0
14-15	0.019326570850085652	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.14758396936061977	0.0
16-17	0.022697893973607615	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.15509122423248214	0.0
18-19	0.028862421504325407	2.2793211037948127E-5	4.144220188717841E-6	0.0	0.16225243671858658	0.0
20-21	0.034494416740792955	2.486532113230705E-5	4.144220188717841E-6	0.0	0.166821439476648	0.0
22-23	0.03698094885402366	3.315376150974273E-5	4.144220188717841E-6	0.0	0.1716577444368817	0.0
24-25	0.042687540053888126	3.315376150974273E-5	4.144220188717841E-6	0.0	0.1760257525157903	0.0
26-27	0.04753420556459364	3.729798169846057E-5	4.144220188717841E-6	0.0	0.17949239270365278	0.0
28-29	0.05946541548791231	3.729798169846057E-5	4.144220188717841E-6	0.0	0.1827808314234004	0.0
30-31	0.08358063276606142	4.144220188717841E-5	4.144220188717841E-6	0.0	0.1868007250064567	0.0
32-33	0.1042022724251214	4.5586422075896254E-5	4.144220188717841E-6	0.0	0.18993168335903304	0.0
34-35	0.11919191684771382	4.973064226461409E-5	4.144220188717841E-6	0.0	0.19334030446425343	0.0
36-37	0.12173232382339788	5.3874862453331936E-5	4.144220188717841E-6	0.0	0.19553881327436828	0.0
38-39	0.12417326951455268	6.009119273640869E-5	4.144220188717841E-6	0.0	0.19721929456089335	0.0
40-41	0.12572942419541622	6.630752301948546E-5	4.144220188717841E-6	0.0	0.19901374190260818	0.0
42-43	0.1260547454802306	6.837963311384438E-5	4.144220188717841E-6	0.0	0.20083719878564404	0.0
44-45	0.12675511869212389	7.04517432082033E-5	4.144220188717841E-6	0.0	0.202181998236883	0.0
46-47	0.12809991814336283	7.04517432082033E-5	4.144220188717841E-6	0.0	0.2037029270461424	0.0
48-49	0.12936183319082742	7.459596339692114E-5	4.144220188717841E-6	0.0	0.2048591644787947	0.0
50-51	0.13033986915536483	7.459596339692114E-5	4.144220188717841E-6	0.0	0.20596981548937107	6.2163302830767615E-6
52-53	0.1313096166795248	7.459596339692114E-5	4.144220188717841E-6	0.0	0.2071447019128726	8.288440377435682E-6
54-55	0.1318297163132089	8.288440377435683E-5	4.144220188717841E-6	0.0	0.20825120870326025	8.288440377435682E-6
56-57	0.13261297392887655	8.702862396307467E-5	4.144220188717841E-6	0.0	0.20928104742015663	8.288440377435682E-6
58-59	0.1327973917272745	9.117284415179251E-5	4.144220188717841E-6	0.0	0.21029016503610942	8.288440377435682E-6
60-61	0.1330999198010509	9.94612845292282E-5	4.144220188717841E-6	0.0	0.21110657641328684	8.288440377435682E-6
62-63	0.13337965466378937	9.94612845292282E-5	6.2163302830767615E-6	0.0	0.2121426314604663	8.288440377435682E-6
64-65	0.1334190247555822	1.0360550471794603E-4	8.288440377435682E-6	0.0	0.21309994632406012	8.288440377435682E-6
66-67	0.13351226970982832	1.0774972490666387E-4	8.288440377435682E-6	0.0	0.21379203109557599	8.288440377435682E-6
68-69	0.13354749558143242	1.1189394509538171E-4	1.0360550471794604E-5	0.0	0.21470790375728266	8.288440377435682E-6
70-71	0.13366767796690526	1.1603816528409956E-4	1.2432660566153525E-5	0.0	0.21550981036379954	8.288440377435682E-6
72-73	0.1337422739303022	1.1811027537845847E-4	1.6576880754871364E-5	0.0	0.21608378485993696	8.288440377435682E-6
74-75	0.13377542769181192	1.2225449556717632E-4	1.6576880754871364E-5	0.0	0.21682560027371744	8.288440377435682E-6
76-77	0.13381065356341604	1.326150460389709E-4	1.6576880754871364E-5	0.0	0.21765444431146103	8.288440377435682E-6
78-79	0.13386245631577498	1.388313763220477E-4	1.6576880754871364E-5	0.0	0.21881689807439636	1.2432660566153525E-5
80-81	0.13392461961860574	1.4297559651076553E-4	1.6576880754871364E-5	0.0	0.21963123734147943	1.2432660566153525E-5
82-83	0.1339474128296437	1.4504770660512444E-4	1.6576880754871364E-5	0.0	0.22052431679214812	1.2432660566153525E-5
84-85	0.1339826387012478	1.4711981669948335E-4	1.6576880754871364E-5	0.0	0.22147334321536452	1.4504770660512445E-5
86-87	0.13403029723341806	1.5126403688820123E-4	1.6576880754871364E-5	0.0	0.22239957642754296	2.0721100943589205E-5
88-89	0.13407588365549394	1.5748036717127796E-4	1.6576880754871364E-5	0.0	0.22332580963972137	2.0721100943589205E-5
90-91	0.13411110952709807	1.6162458735999581E-4	1.6576880754871364E-5	0.0	0.22401167808095418	2.2793211037948127E-5
92-93	0.13412975851794728	1.6162458735999581E-4	1.6576880754871364E-5	0.0	0.22459601312756342	2.900954132102489E-5
94-95	0.13416705649964578	1.6784091764307258E-4	1.6576880754871364E-5	0.0	0.22540413606436338	3.108165141538381E-5
96-97	0.13417741705011754	1.802735782092261E-4	1.6576880754871364E-5	0.0	0.2261708167992762	3.522587160410165E-5
98-99	0.1341898497106837	1.8856201858666178E-4	2.0721100943589205E-5	0.0	0.226775872946829	3.729798169846057E-5
100-101	0.1342105708116273	1.906341286810207E-4	2.0721100943589205E-5	0.0	0.22733327056221153	3.937009179281949E-5
102-103	0.13422093136209906	1.906341286810207E-4	2.4865321132307046E-5	0.0	0.22783679331514076	4.144220188717841E-5
104-105	0.13424372457313705	1.906341286810207E-4	3.315376150974273E-5	0.0	0.22851230120590177	4.144220188717841E-5
106-107	0.13427687833464677	1.9477834886973854E-4	3.315376150974273E-5	0.0	0.2291422226745869	4.144220188717841E-5
108-109	0.13430381576587344	1.989225690584564E-4	3.315376150974273E-5	0.0	0.22983845166629147	4.144220188717841E-5
110-111	0.1343514742980437	2.0513889934153316E-4	3.315376150974273E-5	0.0	0.2304683731349766	4.765853217025518E-5
112-113	0.13437012328889292	2.0928311953025098E-4	3.315376150974273E-5	0.0	0.2310672129522463	5.3874862453331936E-5
114-115	0.13438462805955342	2.134273397189688E-4	3.315376150974273E-5	0.0	0.23165569221904425	5.3874862453331936E-5
116-117	0.13440120494030833	2.1757155990768668E-4	3.315376150974273E-5	4.144220188717841E-6	0.23221930616470987	5.801908264204978E-5
118-119	0.13441778182106318	2.2378789019076342E-4	3.315376150974273E-5	4.144220188717841E-6	0.23278706433056423	6.216330283076762E-5
120-121	0.13443021448162934	2.2378789019076342E-4	3.315376150974273E-5	8.288440377435682E-6	0.23342527423962678	6.630752301948546E-5
122-123	0.13443435870181805	2.300042204738402E-4	3.315376150974273E-5	8.288440377435682E-6	0.2340178977266134	6.630752301948546E-5
124-125	0.13444886347247856	2.3829266085127588E-4	3.522587160410165E-5	8.288440377435682E-6	0.23464574708520416	6.837963311384438E-5
126-127	0.13446544035323343	2.4450899113435265E-4	3.729798169846057E-5	8.288440377435682E-6	0.2353813461687016	7.04517432082033E-5
128-129	0.13447372879361086	2.4450899113435265E-4	3.729798169846057E-5	8.288440377435682E-6	0.23611072892191592	7.04517432082033E-5
130-131	0.13448616145417702	2.527974315117883E-4	3.729798169846057E-5	8.288440377435682E-6	0.23701831314324512	7.04517432082033E-5
132-133	0.13452553154596986	2.527974315117883E-4	3.729798169846057E-5	1.6576880754871364E-5	0.23766895571287383	7.04517432082033E-5
134-135	0.13454625264691344	2.631579819835829E-4	3.729798169846057E-5	1.6576880754871364E-5	0.2382968050714646	7.04517432082033E-5
136-137	0.13455246897719653	2.652300920779418E-4	3.729798169846057E-5	1.6576880754871364E-5	0.23894123131081021	7.04517432082033E-5
138-139	0.13455454108729087	2.6937431226665965E-4	3.729798169846057E-5	1.6576880754871364E-5	0.2396125949813825	7.04517432082033E-5
>>END_MODULE
