##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22878051
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	68-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.44612344819058	36.0	34.0	38.0	31.0	39.0
2	34.742015392832194	35.0	34.0	37.0	31.0	39.0
3	34.92168432529502	35.0	34.0	38.0	31.0	39.0
4	34.88506197490337	36.0	34.0	38.0	31.0	39.0
5	34.76253641536161	35.0	34.0	37.0	31.0	39.0
6	34.7482857696226	35.0	34.0	37.0	31.0	39.0
7	34.74522292130567	35.0	34.0	37.0	32.0	39.0
8	34.89226162665692	35.0	34.0	37.0	32.0	39.0
9	34.87325179054807	35.0	34.0	37.0	32.0	39.0
10-14	34.84968143483901	35.0	34.0	37.0	31.2	39.0
15-19	34.850125974454734	35.0	34.0	37.0	31.0	39.0
20-24	34.85772688416509	35.0	34.0	37.0	31.0	39.0
25-29	34.84639664453935	35.0	34.0	37.0	31.0	39.0
30-34	34.83651746383466	35.0	34.0	37.0	31.0	39.0
35-39	34.830547191279535	35.0	34.0	37.0	31.0	39.0
40-44	34.76596521268355	35.0	34.0	37.0	31.0	39.0
45-49	34.68103171900439	35.0	34.0	37.0	31.0	39.0
50-54	34.642193646652856	35.0	34.0	37.0	31.0	39.0
55-59	34.63385699245098	35.0	34.0	37.0	31.0	39.0
60-64	34.60825919130961	35.0	34.0	37.0	31.0	39.0
65-69	34.589618400420896	35.0	34.0	37.0	31.0	39.0
70-74	34.56509639643283	35.0	34.0	37.0	30.0	39.0
75-79	34.54688510669057	35.0	34.0	37.0	30.0	39.0
80-84	34.52678789528265	35.0	34.0	37.0	30.0	39.0
85-89	34.50650503561632	35.0	34.0	37.0	30.0	39.0
90-94	34.48068761876371	35.0	34.0	37.0	30.0	39.0
95-99	34.453976026083055	35.0	34.0	37.0	30.0	39.0
100-104	34.41747359521629	35.0	34.0	37.0	30.0	39.0
105-109	34.36966012700094	35.0	34.0	37.0	30.0	39.0
110-114	34.33867443317787	35.0	34.0	37.0	30.0	39.0
115-119	34.2953476506753	35.0	34.0	37.0	30.0	39.0
120-124	34.26026411712023	35.0	34.0	37.0	30.0	39.0
125-129	34.21799888532103	35.0	34.0	37.0	29.8	39.0
130-134	34.16410267809154	35.0	34.0	37.0	29.0	39.0
135-139	34.12373258861976	35.0	34.0	37.0	29.0	39.0
140-144	34.091651500096745	35.0	34.0	37.0	29.0	39.0
145-149	34.104884188386144	35.0	34.0	37.0	29.2	39.0
150	35.00034692876958	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	12.0
22	277.0
23	2976.0
24	20052.0
25	79510.0
26	201646.0
27	365562.0
28	536555.0
29	697412.0
30	865513.0
31	1081170.0
32	1378998.0
33	1814781.0
34	2517070.0
35	3960693.0
36	8350111.0
37	1005702.0
38	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.13887175091969	11.428045159965768	14.980415071196408	38.452668017918135
2	22.120135396332373	13.91546558661982	37.068491941173235	26.895907075874575
3	20.91550329116932	18.388752637409176	28.323874262125354	32.37186980929616
4	25.730565078028523	26.27194440223998	22.349620448321545	25.64787007140995
5	25.14612697726659	31.450344889564004	24.607444656117515	18.79608347705189
6	18.710601393564044	36.543155158027155	26.270714742484387	18.475528705924418
7	15.98218323825148	24.101220879948155	40.50024549778309	19.416350384017278
8	18.430526442226576	24.244882469765315	31.63168608762958	25.69290500037853
9	19.658233715207672	23.3044389482452	32.46702338732632	24.570303949220808
10-14	20.33649206410789	29.09556512255027	27.031863561405583	23.536079251936258
15-19	20.413401509773706	27.919837963257198	27.940064406758857	23.72669612021024
20-24	20.571034101316968	28.08256345457388	27.640850818919183	23.705551625189972
25-29	20.524943046305232	28.285770127328686	27.53470615843151	23.65458066793457
30-34	20.52237186401773	28.002169854456326	27.560758577998755	23.91469970352718
35-39	20.599140972640768	28.012438313745854	27.613578768173085	23.774841945440294
40-44	20.655583061251846	28.05699273209637	27.52228405935891	23.765140147292875
45-49	20.74144495882852	27.846248560733766	27.480029835381835	23.93227664505588
50-54	20.842398046660456	27.834652250615864	27.57397805292222	23.748971649801458
55-59	20.845948531982692	27.855335977695074	27.485521523265938	23.813193967056293
60-64	20.791963440984745	27.782944606882953	27.483429663472542	23.94166228865976
65-69	20.910106351516717	27.691502779974307	27.560823417408876	23.8375674511001
70-74	21.013406220431172	27.658930050143244	27.530424805745934	23.79723892367965
75-79	21.03635763853359	27.5295322299342	27.526767998788003	23.9073421327442
80-84	21.083157141798356	27.529118482484588	27.521812755933183	23.86591161978387
85-89	21.058905526868916	27.533873807826414	27.579990808071376	23.827229857233295
90-94	21.05129192267076	27.43084609041758	27.539046064187815	23.97881592272384
95-99	21.135027580121594	27.351462701390144	27.63737461646898	23.876135102019276
100-104	21.187128355523143	27.4056527836053	27.52011525013853	23.887103610733025
105-109	21.1485791891834	27.24241490412166	27.604705598487243	24.004300308207696
110-114	21.227586433594016	27.22793979826916	27.6651337295587	23.87934003857812
115-119	21.258828130291214	27.25642845790823	27.621168414004305	23.863574997796256
120-124	21.2248123386804	27.145670218598912	27.63152396137097	23.997993481349724
125-129	21.276696155453564	27.081561826711457	27.681071971808098	23.960670046026873
130-134	21.338238001089827	27.175287948681948	27.590328987311057	23.896145062917167
135-139	21.32551431913851	27.008731662698683	27.6843748455676	23.98137917259521
140-144	21.37334399354985	26.955552131373278	27.81074602866873	23.86035784640814
145-149	20.99230365833373	27.202791412863387	27.851885367606634	23.95301956119625
150	20.890770056722854	25.822739402715257	28.569526192421478	24.71696434814041
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	2.0
10	2.5
11	4.5
12	12.0
13	18.0
14	37.0
15	75.0
16	158.5
17	322.0
18	633.5
19	1201.5
20	2245.0
21	3719.5
22	5832.0
23	9114.0
24	14450.5
25	22881.5
26	34245.0
27	48322.0
28	65928.5
29	91786.0
30	123633.5
31	156296.5
32	188653.5
33	224820.5
34	262669.5
35	306902.0
36	374682.0
37	465154.5
38	574719.0
39	710507.5
40	868567.5
41	1027803.0
42	1189912.5
43	1355391.0
44	1489516.0
45	1569101.0
46	1590968.0
47	1552921.5
48	1466161.0
49	1342700.0
50	1184978.0
51	1013085.5
52	840756.0
53	672226.5
54	519314.0
55	391092.0
56	293492.5
57	220688.5
58	166418.5
59	124837.5
60	87624.5
61	61037.0
62	44358.0
63	30105.5
64	20807.0
65	16671.0
66	15003.0
67	11346.0
68	6655.0
69	4400.0
70	3708.5
71	2986.0
72	2185.0
73	1285.5
74	632.0
75	194.0
76	56.0
77	34.0
78	19.5
79	3.5
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.355010529524565E-4
3	5.2452020497725084E-5
4	3.05970119570063E-5
5	1.3113005124431271E-5
6	9.179103587101891E-5
7	1.9232407515832532E-4
8	1.0053303928730642E-4
9	8.304903245473139E-5
10-14	1.8970147413343907E-4
15-19	3.4705753562661436E-4
20-24	2.395308936062779E-4
25-29	3.479317359682431E-4
30-34	8.881875470948115E-4
35-39	9.756075812576866E-4
40-44	0.001244861286479342
45-49	0.0010822600229363944
50-54	6.302984463143298E-4
55-59	4.0125795680759694E-4
60-64	2.648827035135117E-4
65-69	3.0334752384890694E-4
70-74	3.1208956015749633E-4
75-79	3.033476590940892E-4
80-84	3.2520299616863664E-4
85-89	3.6803946026427436E-4
90-94	4.0650553271077023E-4
95-99	2.7537602771426304E-4
100-104	4.589632640558156E-4
105-109	2.5352433798675803E-4
110-114	2.168091277482043E-4
115-119	2.1331463624718356E-4
120-124	2.2730561505717158E-4
125-129	1.1278062770829782E-4
130-134	1.739844390590869E-4
135-139	1.4601655259378514E-4
140-144	1.6007093504124647E-4
145-149	1.7743253745210782E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	2.0
70-71	1.0
72-73	1.0
74-75	1.0
76-77	9.0
78-79	5.0
80-81	15.0
82-83	14.0
84-85	16.0
86-87	12.0
88-89	22.0
90-91	34.0
92-93	39.0
94-95	37.0
96-97	64.0
98-99	63.0
100-101	70.0
102-103	68.0
104-105	59.0
106-107	68.0
108-109	80.0
110-111	96.0
112-113	121.0
114-115	99.0
116-117	100.0
118-119	112.0
120-121	151.0
122-123	157.0
124-125	150.0
126-127	219.0
128-129	239.0
130-131	292.0
132-133	389.0
134-135	548.0
136-137	939.0
138-139	2590.0
140-141	5471.0
142-143	9121.0
144-145	71935.0
146-147	728133.0
148-149	1360618.0
150-151	2.0695891E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.467636595778146
#Duplication Level	Percentage of total
1	39.84882220934561
2	10.87531026665968
3	6.443387465615884
4	4.4899456185882745
5	3.3691924385487897
6	2.6445781980807572
7	2.2024010804483454
8	1.9141524251876711
9	1.5409651447756625
>10	17.773022076861043
>50	4.356006278092093
>100	3.9998976637223094
>500	0.3142990102542286
>1k	0.22802012381954612
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.1855008540718788E-5	0.0	0.0	2.6226010248862544E-4	2.6226010248862546E-5
2	4.371001708143758E-6	1.0490404099545018E-4	0.0	0.0	0.0022073558626125975	5.245202049772509E-5
3	1.748400683257503E-5	1.2675904953616897E-4	0.0	0.0	0.0033088482930648247	7.430702903844388E-5
4	4.3710017081437576E-5	1.3987205466060024E-4	0.0	4.371001708143758E-6	0.0047075688396708264	9.616203757916266E-5
5	5.245202049772509E-5	1.3987205466060024E-4	0.0	1.3113005124431273E-5	0.00576972225474976	1.1364604441173769E-4
6	7.867803074658763E-5	1.3987205466060024E-4	0.0	1.3113005124431273E-5	0.006871214685201987	1.1364604441173769E-4
7	8.742003416287515E-5	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.0080513851464008	1.223880478280252E-4
8	1.0053303928730642E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.010372387053425136	1.223880478280252E-4
9	1.1801704611988145E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.013681235346489962	1.3113005124431272E-4
10-11	1.7265456747167843E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.018109060076839587	2.141790836990441E-4
12-13	2.1855008540718786E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.021771959508264056	2.600746016345536E-4
14-15	2.3384859138569103E-4	1.6828356576353468E-4	0.0	1.529850597850315E-5	0.026239123253986977	2.688166050508411E-4
16-17	2.5133259821826605E-4	1.9888057772054097E-4	0.0	1.748400683257503E-5	0.030990402110739244	2.71002105904913E-4
18-19	2.7537310761305674E-4	2.2073558626125976E-4	0.0	1.748400683257503E-5	0.035057619200167006	2.71002105904913E-4
20-21	3.3656713152706937E-4	2.7318760675898484E-4	0.0	1.748400683257503E-5	0.03871614762988333	2.906716135915599E-4
22-23	4.1087416056551323E-4	2.928571144456318E-4	0.0	1.748400683257503E-5	0.04298224529703164	3.1908312469449427E-4
24-25	4.939231930202446E-4	3.037846187159912E-4	0.0	1.748400683257503E-5	0.047722596649513546	3.3001062896485366E-4
26-27	5.267057058313228E-4	3.05970119570063E-4	0.0	1.748400683257503E-5	0.05198432331495371	3.5186563750557245E-4
28-29	5.616737194964728E-4	3.05970119570063E-4	0.0	1.966950768664691E-5	0.056486455074341774	3.6716414348407565E-4
30-31	6.163112408482697E-4	3.05970119570063E-4	0.0	2.6226010248862546E-5	0.06108037786960087	4.0213215714922574E-4
32-33	6.578357570756355E-4	3.0815562042413487E-4	0.0	2.6226010248862546E-5	0.06526342650429444	4.480276750847351E-4
34-35	7.146587792815043E-4	3.1034112127820677E-4	0.0	2.6226010248862546E-5	0.06897222145365442	4.523986767928789E-4
36-37	7.430702903844388E-4	3.1034112127820677E-4	0.0	2.8411511102934423E-5	0.07257611236201894	4.5895517935509456E-4
38-39	7.736673023414451E-4	3.168976238404224E-4	0.0	3.496801366515006E-5	0.07716129315386175	4.633261810632383E-4
40-41	8.151918185688108E-4	3.190831246944943E-4	0.0	3.496801366515006E-5	0.08085916059895137	4.720681844795258E-4
42-43	8.173773194228827E-4	3.2563962725670997E-4	0.0	3.496801366515006E-5	0.08424668692276278	4.7643918618766955E-4
44-45	8.63272837358392E-4	3.365671315270693E-4	0.0	3.496801366515006E-5	0.08844503406343487	4.851811896039571E-4
46-47	9.157248578561172E-4	3.4749463579742876E-4	2.185500854071879E-6	3.496801366515006E-5	0.092614969693004	4.939231930202446E-4
48-49	9.419508681049798E-4	3.496801366515006E-4	4.371001708143758E-6	3.496801366515006E-5	0.09649641920983566	5.092216989987478E-4
50-51	9.659913774997704E-4	3.496801366515006E-4	4.371001708143758E-6	3.496801366515006E-5	0.10057237830267972	5.157782015609634E-4
52-53	0.0010162578971434236	3.5405113835964435E-4	4.371001708143758E-6	4.1524516227365695E-5	0.10491715400057461	5.288912066853947E-4
54-55	0.0010577824133707894	3.6060764092185995E-4	4.371001708143758E-6	4.3710017081437576E-5	0.10839428585940297	5.288912066853947E-4
56-57	0.0010774519210574362	3.6716414348407565E-4	4.371001708143758E-6	4.808101878958133E-5	0.1128527076017096	5.485607143720416E-4
58-59	0.0011036779313062987	3.8901915202479444E-4	4.371001708143758E-6	5.245202049772509E-5	0.11740729138159539	5.616737194964729E-4
60-61	0.001138645944971449	3.9994665629515384E-4	4.371001708143758E-6	5.245202049772509E-5	0.12093687526092148	5.682302220586885E-4
62-63	0.001188912464615102	4.0650315885736943E-4	4.371001708143758E-6	5.245202049772509E-5	0.12470249323248733	5.682302220586885E-4
64-65	0.00122169497742618	4.0650315885736943E-4	4.371001708143758E-6	6.556502562215636E-5	0.12973133069770673	5.682302220586885E-4
66-67	0.0012304369808424677	4.3491466996030387E-4	4.371001708143758E-6	7.430702903844388E-5	0.13380073328798855	5.726012237668323E-4
68-69	0.0012828890013401929	4.545841776469508E-4	4.371001708143758E-6	7.649252989251576E-5	0.13675990144440187	5.813432271831198E-4
70-71	0.0013309700201297743	4.633261810632383E-4	4.371001708143758E-6	8.304903245473139E-5	0.13952893102651096	5.900852305994073E-4
72-73	0.0013637525329408522	4.720681844795258E-4	4.371001708143758E-6	8.523453330880326E-5	0.14224769408897636	5.96641733161623E-4
74-75	0.0014140190525845056	4.7643918618766955E-4	4.371001708143758E-6	8.960553501694704E-5	0.1454538238418998	5.988272340156948E-4
76-77	0.001459914570520015	4.895521913121008E-4	4.371001708143758E-6	9.616203757916266E-5	0.14802615834714242	6.010127348697667E-4
78-79	0.001499253585893309	4.939231930202446E-4	4.371001708143758E-6	1.333155520983846E-4	0.150567895840428	6.031982357238386E-4
80-81	0.0015407781021206746	5.026651964365321E-4	4.371001708143758E-6	1.355010529524565E-4	0.15321672287556315	6.075692374319822E-4
82-83	0.0015779316166398966	5.114071998528196E-4	4.371001708143758E-6	1.355010529524565E-4	0.15591363092948785	6.075692374319822E-4
84-85	0.0016216416337213341	5.179637024150353E-4	4.371001708143758E-6	1.355010529524565E-4	0.158291455858718	6.097547382860541E-4
86-87	0.0016653516508027717	5.267057058313228E-4	4.371001708143758E-6	1.355010529524565E-4	0.16045728720510327	6.206822425564135E-4
88-89	0.0016893921601975623	5.354477092476103E-4	4.371001708143758E-6	1.355010529524565E-4	0.16257503753269892	6.250532442645573E-4
90-91	0.001711247168738281	5.42004211809826E-4	4.371001708143758E-6	1.3987205466060024E-4	0.165405261138722	6.250532442645573E-4
92-93	0.0017352876781330717	5.441897126638978E-4	4.371001708143758E-6	1.464285572228159E-4	0.1681939602285177	6.250532442645573E-4
94-95	0.0018139657088796592	5.485607143720416E-4	4.371001708143758E-6	1.4861405807688776E-4	0.17125147592336426	6.272387451186291E-4
96-97	0.0018970147413343908	5.573027177883291E-4	4.371001708143758E-6	1.4861405807688776E-4	0.17361618784747004	6.316097468267729E-4
98-99	0.0019494667618321158	5.726012237668322E-4	4.371001708143758E-6	1.5079955893095964E-4	0.17574486567933606	6.337952476808448E-4
100-101	0.00197787827293505	5.813432271831198E-4	4.371001708143758E-6	1.529850597850315E-4	0.17823852215383207	6.381662493889886E-4
102-103	0.0019953622797676254	5.813432271831198E-4	4.371001708143758E-6	1.5517056063910339E-4	0.1801945454182264	6.381662493889886E-4
104-105	0.0020325157942868476	5.900852305994073E-4	4.371001708143758E-6	1.5735606149317526E-4	0.18212434267237188	6.381662493889886E-4
106-107	0.0020915243173467877	6.031982357238386E-4	4.371001708143758E-6	1.5954156234724713E-4	0.18389896936587824	6.381662493889886E-4
108-109	0.0022073558626125975	6.031982357238386E-4	4.371001708143758E-6	1.639125640553909E-4	0.18557524852095136	6.381662493889886E-4
110-111	0.002277291889942898	6.075692374319822E-4	4.371001708143758E-6	1.6609806490946278E-4	0.18715536563844534	6.381662493889886E-4
112-113	0.0023515989189813414	6.075692374319822E-4	4.371001708143758E-6	1.6609806490946278E-4	0.18895840384305465	6.425372510971323E-4
114-115	0.0024106074420412824	6.14125739994198E-4	4.371001708143758E-6	1.6828356576353468E-4	0.19076144204766393	6.469082528052761E-4
116-117	0.002445575455706432	6.272387451186292E-4	4.371001708143758E-6	1.748400683257503E-4	0.19246831821469407	6.469082528052761E-4
118-119	0.0025067694796204448	6.556502562215636E-4	4.371001708143758E-6	1.748400683257503E-4	0.19410962935610204	6.490937536593479E-4
120-121	0.0025876330112211045	6.687632613459949E-4	4.371001708143758E-6	1.748400683257503E-4	0.19563292345139016	6.556502562215636E-4
122-123	0.0027362470692979925	6.709487622000667E-4	4.371001708143758E-6	1.748400683257503E-4	0.19714747554326198	6.556502562215636E-4
124-125	0.00283896560943937	6.731342630541386E-4	4.371001708143758E-6	1.748400683257503E-4	0.1987844156829618	6.578357570756355E-4
126-127	0.0028761191239585924	6.731342630541386E-4	4.371001708143758E-6	1.7702556917982218E-4	0.20025962875946032	6.600212579297074E-4
128-129	0.0029242001427481736	6.731342630541386E-4	4.371001708143758E-6	1.7921107003389405E-4	0.20144635572322134	6.600212579297074E-4
130-131	0.002991950669224402	6.862472681785699E-4	4.371001708143758E-6	1.7921107003389405E-4	0.20269646221175047	6.600212579297074E-4
132-133	0.003064072197408774	6.906182698867137E-4	4.371001708143758E-6	1.8139657088796592E-4	0.20378484163707827	6.600212579297074E-4
134-135	0.0032105007546315897	6.971747724489293E-4	4.371001708143758E-6	1.857675725961097E-4	0.20487322106240607	6.600212579297074E-4
136-137	0.0033132192947729682	7.015457741570731E-4	4.371001708143758E-6	1.8795307345018157E-4	0.20600312500396123	6.622067587837793E-4
138-139	0.003330703301605543	7.168442801355763E-4	4.371001708143758E-6	1.8795307345018157E-4	0.20706090741733202	6.643922596378511E-4
>>END_MODULE
