##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22878051
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.073856378762336	37.0	35.0	38.0	33.0	39.0
2	35.37427191678172	35.0	34.0	37.0	33.0	39.0
3	35.53325932353241	35.0	35.0	38.0	33.0	39.0
4	35.5856708248443	36.0	35.0	38.0	33.0	39.0
5	35.49822115528985	36.0	35.0	38.0	33.0	39.0
6	35.444006921743465	35.0	35.0	37.0	33.0	39.0
7	35.47330710120368	35.0	34.0	38.0	33.0	39.0
8	35.56549624791028	36.0	35.0	38.0	33.0	39.0
9	35.57059375381233	36.0	35.0	38.0	33.0	39.0
10-14	35.55739028643656	35.8	35.0	37.8	33.0	39.0
15-19	35.530498913565665	36.0	35.0	38.0	33.0	39.0
20-24	35.517484850435906	36.0	35.0	38.0	33.0	39.0
25-29	35.49656805992783	36.0	35.0	38.0	33.0	39.0
30-34	35.4802712608692	36.0	35.0	38.0	33.0	39.0
35-39	35.451987951246366	36.0	34.8	38.0	33.0	39.0
40-44	35.437027935640145	36.0	34.4	38.0	33.0	39.0
45-49	35.406718343271464	36.0	34.0	38.0	33.0	39.0
50-54	35.386322873395116	36.0	34.0	38.0	33.0	39.0
55-59	35.35992961113689	36.0	34.0	38.0	32.8	39.0
60-64	35.320189672793404	36.0	34.0	38.0	32.0	39.0
65-69	35.29475788057276	36.0	34.0	38.0	32.0	39.0
70-74	35.265752362197865	36.0	34.0	38.0	32.0	39.0
75-79	35.21998637996578	36.0	34.0	38.0	32.0	39.0
80-84	35.17965768724717	36.0	34.0	38.0	32.0	39.0
85-89	35.13378433907699	35.8	34.0	38.0	32.0	39.0
90-94	35.07855055378743	35.4	34.0	38.0	32.0	39.0
95-99	35.017026887036465	35.0	34.0	37.0	32.0	39.0
100-104	34.97053046944882	35.0	34.0	37.0	31.4	39.0
105-109	34.913231921177285	35.0	34.0	37.0	31.0	39.0
110-114	34.840665437004084	35.0	34.0	37.0	31.0	39.0
115-119	34.78255518458855	35.0	34.0	37.0	31.0	39.0
120-124	34.71073154557142	35.0	34.0	37.0	31.0	39.0
125-129	34.6366691114726	35.0	34.0	37.0	30.0	39.0
130-134	34.53968467097344	35.0	34.0	37.0	30.0	39.0
135-139	34.48590237119688	35.0	34.0	37.0	30.0	39.0
140-144	34.366104786782785	35.0	34.0	37.0	30.0	39.0
145-149	34.3575435212015	35.0	34.0	37.0	30.0	39.0
150	35.10834477705239	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	134.0
22	1304.0
23	7171.0
24	25262.0
25	63121.0
26	128448.0
27	224734.0
28	334935.0
29	444241.0
30	574071.0
31	747876.0
32	1005890.0
33	1414804.0
34	2150462.0
35	3739529.0
36	1.0307847E7
37	1708212.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.17649956283426	18.513941594063237	15.706652634002783	31.602906209099718
2	24.13751940670121	25.45695435332319	33.706695557239556	16.698830682736045
3	19.89472965985867	29.206415284799853	29.69125557865533	21.207599476686145
4	23.653883248523506	34.036119076412504	22.21831940302252	20.091678272041467
5	25.173527720572274	35.138111669943825	22.4967386854952	17.1916219239887
6	18.98494485626121	38.617895738216596	23.18628161483149	19.2108777906907
7	20.317295991612372	20.475525672535152	38.02498463228264	21.182193703569833
8	21.610577467982655	23.189868611792004	28.49687272416651	26.702681196058837
9	23.505109766561848	23.24153399255907	28.86325850047279	24.390097740406297
10-14	23.31281191741377	28.99102025780081	25.615735361373222	22.0804324634122
15-19	23.0604040527753	28.2562959580779	27.07189611562628	21.611403873520523
20-24	23.17672777283345	28.327684032175643	26.92220941373022	21.573378781260693
25-29	23.24150689234848	28.330053989301803	26.878417221816665	21.550021896533057
30-34	23.244750175615923	28.076843608749712	27.0651953700077	21.613210845626664
35-39	23.29360419114027	28.100326623979697	27.059721364024597	21.546347820855438
40-44	23.457146969880842	27.860682956434385	27.143764363002465	21.53840571068231
45-49	23.454153502848648	27.68366850830082	27.242679020166534	21.619498968684002
50-54	23.518033070212145	27.67911392452093	27.198845740836923	21.60400726443
55-59	23.6891079576665	27.573778902757056	27.093585900302436	21.64352723927401
60-64	23.600705528637082	27.538149360171886	27.180709818163358	21.68043529302767
65-69	23.677436691644818	27.458672979536637	27.141334697692244	21.7225556311263
70-74	23.75347016293237	27.4408283467594	27.057803275075354	21.74789821523288
75-79	23.676710581272076	27.467993454425603	27.144052440056342	21.71124352424598
80-84	23.784688581996242	27.517736782055152	27.02562888852722	21.671945747421383
85-89	23.80085017738707	27.44905066520741	27.057624348194686	21.692474809210836
90-94	23.718546023978174	27.389285436595596	27.14742864532274	21.74473989410349
95-99	23.803135961669184	27.486870519188646	27.145211849706342	21.564781669435824
100-104	23.955231528288426	27.459249709518797	26.96951745264286	21.616001309549915
105-109	23.753204012794072	27.58376279500335	27.06322005320646	21.59981313899612
110-114	23.771135263403465	27.569551490061432	27.019478643441342	21.639834603093764
115-119	23.84756058425496	27.565538199980903	27.034751257220268	21.55214995854387
120-124	23.818062312679803	27.47319665548077	27.217157595812612	21.49158343602682
125-129	23.780461600940125	27.567941809628294	27.1719857315006	21.47961085793098
130-134	23.836707656435838	27.492132372711573	27.220481782626948	21.450678188225638
135-139	23.763697470270216	27.48232216984039	27.327308385303745	21.426671974585652
140-144	23.771324220453643	27.63219155534146	27.30122189852684	21.295262325678056
145-149	23.512446906148675	27.794363496927044	27.202350012769795	21.490839584154493
150	23.69606737331513	26.467078127083	27.6454816659925	22.19137283360937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	0.0
7	1.5
8	2.5
9	3.0
10	4.0
11	5.5
12	7.0
13	10.5
14	23.0
15	38.5
16	61.5
17	118.5
18	192.0
19	314.5
20	497.0
21	800.0
22	1260.0
23	2209.0
24	3723.0
25	6130.0
26	9766.0
27	15571.5
28	25984.0
29	39626.5
30	54094.0
31	71747.0
32	95172.5
33	125985.0
34	169434.5
35	233930.5
36	327681.0
37	443218.5
38	578666.0
39	751477.5
40	955320.0
41	1148352.0
42	1325782.5
43	1489449.5
44	1606080.5
45	1662749.5
46	1664442.0
47	1613023.5
48	1514333.0
49	1374433.0
50	1194424.0
51	1000749.0
52	815521.5
53	647552.0
54	505800.5
55	383173.5
56	283547.5
57	209941.5
58	153962.0
59	111306.5
60	77198.0
61	52096.0
62	35840.0
63	25434.0
64	18043.5
65	13004.5
66	9795.0
67	7384.0
68	6375.0
69	5019.5
70	3146.5
71	2302.5
72	1626.5
73	1222.0
74	1014.5
75	623.0
76	235.0
77	71.5
78	21.0
79	2.5
80	2.0
81	1.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	8.742003416287515E-6
4	8.742003416287515E-6
5	2.6226010248862542E-5
6	1.0053303928730642E-4
7	0.002395308936062779
8	0.11597578832217832
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.742003416287515E-7
40-44	8.742003416287515E-7
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.742005021162908E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	8.742211902177061E-7
95-99	0.0
100-104	1.7484910958965186E-6
105-109	5.0707100018635736E-5
110-114	0.019837368722453085
115-119	0.0
120-124	0.0
125-129	2.6230594343998363E-6
130-134	0.048720613256980384
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	1.0
64-65	3.0
66-67	1.0
68-69	4.0
70-71	7.0
72-73	17.0
74-75	14.0
76-77	23.0
78-79	26.0
80-81	48.0
82-83	42.0
84-85	59.0
86-87	74.0
88-89	106.0
90-91	118.0
92-93	125.0
94-95	100.0
96-97	130.0
98-99	123.0
100-101	172.0
102-103	135.0
104-105	155.0
106-107	170.0
108-109	200.0
110-111	168.0
112-113	218.0
114-115	200.0
116-117	206.0
118-119	247.0
120-121	289.0
122-123	286.0
124-125	333.0
126-127	384.0
128-129	445.0
130-131	506.0
132-133	699.0
134-135	934.0
136-137	1364.0
138-139	3131.0
140-141	6623.0
142-143	9556.0
144-145	64956.0
146-147	791903.0
148-149	960378.0
150-151	2.1033372E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.419392105317286
#Duplication Level	Percentage of total
1	30.0164517180849
2	10.942116342576462
3	6.919982145248027
4	5.052117682638749
5	3.8741995765360993
6	3.114990343066503
7	2.407693370822122
8	2.03312957458089
9	1.7953855042420683
>10	21.025898338519593
>50	5.188582986856547
>100	5.90532761417774
>500	0.9075636944776169
>1k	0.7091079672156364
>5k	0.10745314095691225
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.748400683257503E-5	1.3113005124431273E-5	0.0	0.0	6.906182698867137E-4	0.0
2	3.496801366515006E-5	6.11940239140126E-5	0.0	0.0	0.004287952675689026	0.0
3	5.6823022205868845E-5	6.11940239140126E-5	0.0	0.0	0.005774093256457904	0.0
4	1.0053303928730642E-4	6.11940239140126E-5	0.0	0.0	0.007006715738154443	0.0
5	1.0053303928730642E-4	6.11940239140126E-5	0.0	0.0	0.008645841378708352	0.0
6	1.1364604441173769E-4	6.11940239140126E-5	0.0	0.0	0.010145094964601661	0.0
7	1.2675904953616897E-4	8.304903245473139E-5	0.0	0.0	0.012125158738390783	0.0
8	1.8795307345018157E-4	8.304903245473139E-5	0.0	0.0	0.017081874675425806	0.0
9	1.8795307345018157E-4	9.179103587101891E-5	0.0	0.0	0.022519400800356637	0.0
10-11	2.0325157942868472E-4	1.0490404099545018E-4	0.0	2.185500854071879E-6	0.028269453547419752	0.0
12-13	2.513325982182661E-4	1.0927504270359393E-4	0.0	4.371001708143758E-6	0.033879634239822265	0.0
14-15	3.147121229863506E-4	1.2020254697395333E-4	0.0	4.371001708143758E-6	0.04085356746516563	0.0
16-17	3.824626494625788E-4	1.223880478280252E-4	0.0	6.556502562215636E-6	0.04636977162084305	2.185500854071879E-6
18-19	4.742536853335977E-4	1.3113005124431272E-4	0.0	8.742003416287515E-6	0.05378517601870894	4.371001708143758E-6
20-21	5.245202049772509E-4	1.4205755551467212E-4	0.0	8.742003416287515E-6	0.05927515416413749	4.371001708143758E-6
22-23	5.813432271831198E-4	1.5079955893095964E-4	4.371001708143758E-6	8.742003416287515E-6	0.06486129434714522	4.371001708143758E-6
24-25	6.49093753659348E-4	1.573560614931753E-4	8.742003416287515E-6	8.742003416287515E-6	0.06972621924830921	4.371001708143758E-6
26-27	7.037312750111449E-4	1.6828356576353468E-4	8.742003416287515E-6	8.742003416287515E-6	0.07347872421475063	4.371001708143758E-6
28-29	8.676438390665358E-4	1.7046906661760656E-4	8.742003416287515E-6	8.742003416287515E-6	0.07734050422389566	4.371001708143758E-6
30-31	0.0011495734492418083	1.748400683257503E-4	1.0927504270359394E-5	8.742003416287515E-6	0.08188853150121922	4.371001708143758E-6
32-33	0.0013571960303786369	1.857675725961097E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.08640377626573173	4.371001708143758E-6
34-35	0.0015298505978503152	1.9232407515832532E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.08920777386150595	4.371001708143758E-6
36-37	0.0015801171174939684	2.0106607857461284E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.0923592660930776	4.371001708143758E-6
38-39	0.001623827134575406	2.0325157942868472E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.09546486280671374	4.371001708143758E-6
40-41	0.001678464655927203	2.0543708028275662E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.09810713333928664	4.371001708143758E-6
42-43	0.00169813416361385	2.076225811368285E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.10096358295555857	4.371001708143758E-6
44-45	0.0017440296815493593	2.16364584553116E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.10316001131390082	4.371001708143758E-6
46-47	0.001785554197776725	2.3166309053161916E-4	1.3113005124431273E-5	1.748400683257503E-5	0.1055859172619206	4.371001708143758E-6
48-49	0.0018423772199825939	2.360340922397629E-4	1.3113005124431273E-5	1.748400683257503E-5	0.10772333709720291	8.742003416287515E-6
50-51	0.0019035712438966064	2.360340922397629E-4	1.3113005124431273E-5	1.748400683257503E-5	0.10971651387611644	8.742003416287515E-6
52-53	0.0019472812609780438	2.4259059480197855E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11170969065503	8.742003416287515E-6
54-55	0.0019953622797676254	2.7318760675898484E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11344716383401715	8.742003416287515E-6
56-57	0.0020827823139305006	2.863006118834161E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11541411460268183	8.742003416287515E-6
58-59	0.0021352343344282257	3.05970119570063E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11721715280729114	8.742003416287515E-6
60-61	0.0022139123651748133	3.278251281107818E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.118642099364146	8.742003416287515E-6
62-63	0.002255436881402179	3.365671315270693E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.1206090501328107	1.0927504270359394E-5
64-65	0.002288219394213257	3.5623663921371626E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12223724826909424	1.3113005124431273E-5
66-67	0.0023734539275220602	3.715351451922194E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12347861275420707	1.3113005124431273E-5
68-69	0.002401865438624995	3.7809164775443505E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.124844550788002	1.3113005124431273E-5
70-71	0.0024237204471657136	3.8901915202479444E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12612306878763405	1.748400683257503E-5
72-73	0.00245431745912272	4.15245162273657E-4	1.529850597850315E-5	2.1855008540718788E-5	0.12715681069161006	1.748400683257503E-5
74-75	0.0024870999719337978	4.1743066312772883E-4	1.748400683257503E-5	2.6226010248862546E-5	0.12822770611010526	1.748400683257503E-5
76-77	0.002504583978766373	4.4584217423066327E-4	1.748400683257503E-5	2.6226010248862546E-5	0.12958271663962984	1.748400683257503E-5
78-79	0.0025329954898693076	4.5895517935509456E-4	1.748400683257503E-5	2.6226010248862546E-5	0.13133767382544956	1.748400683257503E-5
80-81	0.002554850498410026	4.6769718277138206E-4	1.748400683257503E-5	2.6226010248862546E-5	0.1327954028951155	2.1855008540718788E-5
82-83	0.002572334505242601	4.7643918618766955E-4	1.748400683257503E-5	2.6226010248862546E-5	0.13441485902798278	2.1855008540718788E-5
84-85	0.0026029315171996075	4.829956887498852E-4	1.748400683257503E-5	2.6226010248862546E-5	0.13596875013522786	2.1855008540718788E-5
86-87	0.0026160445223240387	4.895521913121008E-4	1.966950768664691E-5	2.6226010248862546E-5	0.13766251329713358	2.1855008540718788E-5
88-89	0.0026204155240321827	4.939231930202446E-4	2.4040509394790665E-5	2.6226010248862546E-5	0.13924918691718977	2.1855008540718788E-5
90-91	0.002631343028302542	5.092216989987478E-4	2.6226010248862546E-5	2.6226010248862546E-5	0.14026107381262504	2.1855008540718788E-5
92-93	0.0026357140300106857	5.22334704123179E-4	2.8411511102934423E-5	2.6226010248862546E-5	0.1412817027114766	2.6226010248862546E-5
94-95	0.002648827035135117	5.376332101016822E-4	3.05970119570063E-5	2.6226010248862546E-5	0.1422542505915386	3.05970119570063E-5
96-97	0.002653198036843261	5.507462152261134E-4	3.7153514519221934E-5	2.6226010248862546E-5	0.14316123344597842	3.05970119570063E-5
98-99	0.0026597545394054763	5.638592203505448E-4	3.9339015373293815E-5	2.6226010248862546E-5	0.1440485467927316	3.496801366515006E-5
100-101	0.002672867544529908	5.660447212046166E-4	3.9339015373293815E-5	2.6226010248862546E-5	0.14483532710019748	3.496801366515006E-5
102-103	0.0026772385462380515	5.726012237668323E-4	3.9339015373293815E-5	3.05970119570063E-5	0.14573793895292916	3.496801366515006E-5
104-105	0.002685980549654339	5.747867246209042E-4	3.9339015373293815E-5	3.05970119570063E-5	0.14657061477833055	3.496801366515006E-5
106-107	0.0027143920607572733	5.988272340156948E-4	3.9339015373293815E-5	3.05970119570063E-5	0.14737050809092087	3.496801366515006E-5
108-109	0.0027143920607572733	6.11940239140126E-4	4.1524516227365695E-5	3.05970119570063E-5	0.14815291739667857	3.496801366515006E-5
110-111	0.002723134064173561	6.359807485349167E-4	4.808101878958133E-5	3.05970119570063E-5	0.14907519875709693	3.496801366515006E-5
112-113	0.0027253195650276327	6.381662493889886E-4	4.808101878958133E-5	3.05970119570063E-5	0.14984449505773023	3.496801366515006E-5
114-115	0.0027296905667357767	6.425372510971323E-4	4.808101878958133E-5	3.05970119570063E-5	0.15067279988142346	3.7153514519221934E-5
116-117	0.0027318760675898485	6.469082528052761E-4	4.808101878958133E-5	3.05970119570063E-5	0.15156666973073887	4.3710017081437576E-5
118-119	0.0027318760675898485	6.578357570756355E-4	5.245202049772509E-5	3.05970119570063E-5	0.15238186154930766	4.808101878958133E-5
120-121	0.0027340615684439203	6.709487622000667E-4	5.245202049772509E-5	3.05970119570063E-5	0.15326261839349864	4.808101878958133E-5
122-123	0.0027384325701520643	6.796907656163543E-4	5.245202049772509E-5	3.05970119570063E-5	0.15398164817448828	4.808101878958133E-5
124-125	0.002742803571860208	6.862472681785699E-4	5.245202049772509E-5	3.05970119570063E-5	0.15486459051953333	4.808101878958133E-5
126-127	0.002751545575276495	6.928037707407855E-4	5.245202049772509E-5	3.05970119570063E-5	0.15588959042009304	4.808101878958133E-5
128-129	0.002758102077838711	7.102877775733606E-4	5.4637521351796965E-5	3.05970119570063E-5	0.1567878312711166	4.808101878958133E-5
130-131	0.002762473079546855	7.124732784274325E-4	5.6823022205868845E-5	3.05970119570063E-5	0.15786091219046589	5.026651964365321E-5
132-133	0.002782142587233502	7.124732784274325E-4	5.6823022205868845E-5	3.05970119570063E-5	0.1587285560295324	5.245202049772509E-5
134-135	0.0028192961017527236	7.124732784274325E-4	5.6823022205868845E-5	3.496801366515006E-5	0.15947162631991685	5.245202049772509E-5
136-137	0.0028236671034608672	7.146587792815043E-4	5.6823022205868845E-5	3.9339015373293815E-5	0.16031960065129675	5.6823022205868845E-5
138-139	0.0028236671034608672	7.277717844059356E-4	5.6823022205868845E-5	3.9339015373293815E-5	0.16115446197755218	5.6823022205868845E-5
>>END_MODULE
