##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24083933
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00247957839776	37.0	35.0	38.0	33.0	39.0
2	35.342509879927	35.0	34.0	37.0	33.0	39.0
3	35.48684386391542	35.0	34.0	37.0	33.0	39.0
4	35.54886309474453	36.0	35.0	38.0	33.0	39.0
5	35.461856126239844	36.0	35.0	37.0	33.0	39.0
6	35.41954729736211	35.0	35.0	37.0	33.0	39.0
7	35.43971837988422	35.0	34.0	37.0	33.0	39.0
8	35.569723225853515	36.0	35.0	38.0	33.0	39.0
9	35.5271244526382	36.0	35.0	38.0	33.0	39.0
10-14	35.51368346689886	35.8	35.0	37.8	33.0	39.0
15-19	35.488379750931884	36.0	35.0	38.0	33.0	39.0
20-24	35.474036337835685	36.0	34.8	38.0	33.0	39.0
25-29	35.45049525756445	36.0	34.4	38.0	33.0	39.0
30-34	35.43404659861825	36.0	34.0	38.0	33.0	39.0
35-39	35.40319259316989	36.0	34.0	38.0	33.0	39.0
40-44	35.38704885119885	36.0	34.0	38.0	33.0	39.0
45-49	35.35407149654502	36.0	34.0	38.0	32.8	39.0
50-54	35.3277609433642	36.0	34.0	38.0	32.4	39.0
55-59	35.3063068145888	36.0	34.0	38.0	32.0	39.0
60-64	35.26177223628715	36.0	34.0	38.0	32.0	39.0
65-69	35.232837909086115	36.0	34.0	38.0	32.0	39.0
70-74	35.194905355147256	36.0	34.0	38.0	32.0	39.0
75-79	35.15127234054207	35.6	34.0	37.6	32.0	39.0
80-84	35.10924972685076	35.4	34.0	37.4	32.0	39.0
85-89	35.05636140460849	35.0	34.0	37.0	32.0	39.0
90-94	34.99671692344212	35.0	34.0	37.0	32.0	39.0
95-99	34.92858429484837	35.0	34.0	37.0	31.0	39.0
100-104	34.87982282490584	35.0	34.0	37.0	31.0	39.0
105-109	34.81603327448737	35.0	34.0	37.0	31.0	39.0
110-114	34.74084483330033	35.0	34.0	37.0	31.0	39.0
115-119	34.67060159893398	35.0	34.0	37.0	30.4	39.0
120-124	34.58923993073248	35.0	34.0	37.0	30.0	39.0
125-129	34.506259878853555	35.0	34.0	37.0	30.0	39.0
130-134	34.41927565656785	35.0	34.0	37.0	30.0	39.0
135-139	34.340137244732716	35.0	34.0	37.0	29.8	39.0
140-144	34.21065887670612	35.0	34.0	37.0	29.0	39.0
145-149	34.196700008280324	35.0	34.0	37.0	29.0	39.0
150	34.99481989659627	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	13.0
21	154.0
22	1474.0
23	8320.0
24	29938.0
25	74380.0
26	146684.0
27	247268.0
28	364964.0
29	486644.0
30	631757.0
31	830286.0
32	1118585.0
33	1570820.0
34	2364676.0
35	4050782.0
36	1.0501296E7
37	1655885.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.61470902613789	18.249984336030163	16.237563856368475	31.897742781463478
2	24.18923852678049	25.276768541085048	33.537558005995116	16.996434926139347
3	19.677172328954743	29.23415374058714	30.15092260886127	20.93775132159685
4	23.325974208614515	34.07709612877598	22.308690196073872	20.288239466535636
5	25.0117080129728	35.16035358510589	22.49624677165478	17.331691630266537
6	18.683642742238156	38.730260543408754	23.124221446721346	19.461875267631747
7	20.097340413627624	20.319820687094587	38.31315674229786	21.269682156979925
8	21.445376052158924	22.996414248453522	28.539786255010757	27.0184234443768
9	23.429811069479392	23.135436392386577	28.756308199329407	24.67844433880463
10-14	23.112543121590647	28.954496759312526	25.72887576128035	22.204084357816477
15-19	22.930785432761336	28.137715712794915	27.185890277970792	21.745608576472954
20-24	23.029374811829946	28.20448553813864	27.048269068013102	21.71787058201831
25-29	23.113399294043877	28.205792633620096	26.969324320907223	21.711483751428805
30-34	23.110920463032347	27.976021192219726	27.160403576940695	21.752654767807233
35-39	23.189264172004336	27.968178851586895	27.13536636342051	21.70719061298826
40-44	23.352528011101843	27.74178204199455	27.22118268639927	21.684507260504336
45-49	23.356870325125055	27.581955156576793	27.29313688092389	21.76803763737426
50-54	23.43696790251798	27.579497315322186	27.233185935479774	21.750348846680055
55-59	23.604006040043377	27.46505896690545	27.13167820222719	21.79925679082399
60-64	23.516005463061205	27.449522467945748	27.198740338631566	21.83573173036148
65-69	23.613606008992868	27.369105308870406	27.15285903470552	21.864429647431205
70-74	23.68189893771344	27.347521286384836	27.066439918379054	21.90413985752267
75-79	23.595918780698177	27.38587522501045	27.146332437035376	21.87187355725599
80-84	23.71677673097906	27.429683640429136	27.0119231194349	21.841616509156903
85-89	23.74453723596798	27.35857377447914	27.052728828623067	21.844160160929817
90-94	23.669033314829	27.299490967448186	27.14327114516865	21.888204572554155
95-99	23.755669878792094	27.404230284553165	27.12867188942207	21.71142794723267
100-104	23.903915599342128	27.377874978421225	26.942593938556826	21.775615483679818
105-109	23.701193744463193	27.511860786532118	27.051001143323106	21.73594432568158
110-114	23.728198803384952	27.501214093004734	26.99778500436201	21.7728020992483
115-119	23.796685647519173	27.497541607221216	27.022152950608685	21.68361979465093
120-124	23.757690639182254	27.410189858964056	27.198361531817657	21.633757970036033
125-129	23.7459633972155	27.490757831757133	27.157602639121365	21.605676131906
130-134	23.793628542843173	27.42293830930685	27.205534287248135	21.577898860601845
135-139	23.710346133844624	27.432906557319853	27.31144237111569	21.545304937719834
140-144	23.73268213916632	27.561532855179806	27.284290657733944	21.42149434791993
145-149	23.463439400032936	27.669236575431892	27.234643305194734	21.632680719340442
150	23.60192226462284	26.451031211668273	27.654873748479407	22.292172775229474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	3.5
9	6.5
10	6.5
11	5.0
12	7.5
13	11.0
14	18.0
15	39.5
16	69.0
17	128.5
18	201.5
19	332.0
20	500.5
21	793.5
22	1279.0
23	2220.5
24	3753.5
25	6001.5
26	9336.0
27	14940.5
28	25148.0
29	38849.5
30	53085.0
31	70488.5
32	94658.0
33	127233.0
34	172689.0
35	239694.5
36	337425.0
37	458743.0
38	601839.5
39	784473.5
40	1000045.5
41	1204032.0
42	1392075.5
43	1569591.0
44	1695951.0
45	1754734.0
46	1757519.0
47	1706221.5
48	1604136.5
49	1457869.5
50	1267670.5
51	1061782.5
52	864640.5
53	686460.5
54	536496.0
55	406529.5
56	302894.5
57	224941.0
58	163276.0
59	117140.5
60	80990.0
61	54004.0
62	36727.0
63	26043.5
64	18424.0
65	13203.5
66	9740.0
67	7197.0
68	6242.5
69	4806.5
70	2715.0
71	1849.5
72	1346.0
73	1055.5
74	946.0
75	550.5
76	165.0
77	65.0
78	18.5
79	0.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.304291495911404E-7
40-44	0.0
45-49	0.0
50-54	8.304291495911404E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.304718732879303E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.305831065509219E-7
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	4.0
68-69	4.0
70-71	9.0
72-73	11.0
74-75	10.0
76-77	21.0
78-79	26.0
80-81	49.0
82-83	50.0
84-85	62.0
86-87	89.0
88-89	112.0
90-91	123.0
92-93	96.0
94-95	96.0
96-97	135.0
98-99	161.0
100-101	183.0
102-103	171.0
104-105	161.0
106-107	201.0
108-109	194.0
110-111	203.0
112-113	213.0
114-115	233.0
116-117	260.0
118-119	275.0
120-121	328.0
122-123	369.0
124-125	388.0
126-127	435.0
128-129	522.0
130-131	673.0
132-133	820.0
134-135	1232.0
136-137	1624.0
138-139	3535.0
140-141	7152.0
142-143	11232.0
144-145	71227.0
146-147	836915.0
148-149	1060792.0
150-151	2.2083536E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.26156569474258
#Duplication Level	Percentage of total
1	28.991108637714387
2	10.529466573210854
3	7.010738180377617
4	4.940230839844963
5	4.025633901014544
6	3.134469027429111
7	2.513277471217021
8	2.1677464011662275
9	1.869549922555186
>10	20.922956532687557
>50	5.485855263746381
>100	6.398402174174158
>500	1.0525171809549732
>1k	0.8580551265352083
>5k	0.09999276737170874
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.0760728739778507E-5	0.0	0.0	6.062132792015324E-4	0.0
2	2.0760728739778507E-5	3.3217165983645615E-5	0.0	0.0	0.004272557974646417	0.0
3	4.5673603227512715E-5	3.7369311731601315E-5	0.0	0.0	0.005675983237455444	0.0
4	1.1210793519480394E-4	3.7369311731601315E-5	0.0	4.152145747955702E-6	0.0069506919820778446	0.0
5	1.2041222669071534E-4	3.7369311731601315E-5	0.0	4.152145747955702E-6	0.00817142283197682	0.0
6	1.2456437243867104E-4	3.7369311731601315E-5	0.0	4.152145747955702E-6	0.00967865173848474	0.0
7	1.5362939267436095E-4	6.228218621933552E-5	4.152145747955702E-6	4.152145747955702E-6	0.011675833843251432	0.0
8	2.1591157889369646E-4	6.228218621933552E-5	4.152145747955702E-6	4.152145747955702E-6	0.01673729951000943	0.0
9	2.2006372464165218E-4	6.643433196729123E-5	4.152145747955702E-6	4.152145747955702E-6	0.022014676755661128	0.0
10-11	2.6366125499518707E-4	6.643433196729123E-5	4.152145747955702E-6	4.152145747955702E-6	0.027615921369653372	0.0
12-13	3.031066396007662E-4	7.681469633718048E-5	4.152145747955702E-6	4.152145747955702E-6	0.03320263347352777	0.0
14-15	3.3424773271043397E-4	8.719506070706972E-5	4.152145747955702E-6	4.152145747955702E-6	0.040483421042568093	0.0
16-17	3.8199740881192455E-4	8.927113358104757E-5	4.152145747955702E-6	4.152145747955702E-6	0.045756646142471824	0.0
18-19	4.4843174077921575E-4	9.965149795093683E-5	4.152145747955702E-6	4.152145747955702E-6	0.05286304359009801	0.0
20-21	5.14866072746507E-4	1.0172757082491468E-4	4.152145747955702E-6	4.152145747955702E-6	0.05833141954015567	0.0
22-23	5.709200403439089E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.0641029021298141	0.0
24-25	6.290500808152888E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.06914775921358027	2.076072873977851E-6
26-27	7.037887042784913E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.07281410390902515	4.152145747955702E-6
28-29	8.34581295339096E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.0765697197380511	4.152145747955702E-6
30-31	0.0010193517811231247	1.307925910606046E-4	1.6608582991822807E-5	4.152145747955702E-6	0.08134053520245219	4.152145747955702E-6
32-33	0.001210350485529087	1.4117295543049385E-4	2.0760728739778507E-5	4.152145747955702E-6	0.08583730904748821	4.152145747955702E-6
34-35	0.001376436315447315	1.4532510117844954E-4	2.0760728739778507E-5	4.152145747955702E-6	0.08865246386460218	4.152145747955702E-6
36-37	0.0014241859915488056	1.4740117405242739E-4	2.0760728739778507E-5	4.152145747955702E-6	0.09175826888407304	4.152145747955702E-6
38-39	0.0014636313761543847	1.5362939267436095E-4	2.0760728739778507E-5	4.152145747955702E-6	0.09471044451086955	4.152145747955702E-6
40-41	0.0015238374894997425	1.6193368417027237E-4	2.4912874487734208E-5	4.152145747955702E-6	0.09741764353853666	4.152145747955702E-6
42-43	0.0015591307283573659	1.764661942881173E-4	2.4912874487734208E-5	4.152145747955702E-6	0.10019750511679301	4.152145747955702E-6
44-45	0.0016006521858369228	1.9515085015391797E-4	2.4912874487734208E-5	6.228218621933553E-6	0.10242513131057124	4.152145747955702E-6
46-47	0.001669162590678192	2.2006372464165218E-4	2.4912874487734208E-5	8.304291495911404E-6	0.10489981017635283	4.152145747955702E-6
48-49	0.0017314447768975276	2.2629194326358572E-4	2.4912874487734208E-5	8.304291495911404E-6	0.10742223871823593	6.228218621933553E-6
50-51	0.0017895748173689073	2.2836801613756357E-4	2.4912874487734208E-5	8.304291495911404E-6	0.10939658402138887	8.304291495911404E-6
52-53	0.0018705416594540435	2.2836801613756357E-4	2.4912874487734208E-5	8.304291495911404E-6	0.1113709293245418	1.0380364369889254E-5
54-55	0.0019452802829172462	2.4082445338143068E-4	2.698894736171206E-5	8.304291495911404E-6	0.11319164523502037	1.2456437243867104E-5
56-57	0.0020241710521284044	2.4705267200336424E-4	2.906502023568991E-5	8.304291495911404E-6	0.11519297948553503	1.2456437243867104E-5
58-59	0.0020760728739778506	2.5120481775131994E-4	2.906502023568991E-5	1.2456437243867104E-5	0.1169098917523147	1.2456437243867104E-5
60-61	0.0021611918618109427	2.5535696349927563E-4	2.906502023568991E-5	1.2456437243867104E-5	0.11829463235925794	1.2456437243867104E-5
62-63	0.002211017610786411	2.6158518212120917E-4	2.906502023568991E-5	1.2456437243867104E-5	0.12023576049642723	1.2456437243867104E-5
64-65	0.0022442347767700566	2.740416193650763E-4	3.114109310966776E-5	1.2456437243867104E-5	0.12184471697376006	1.4532510117844956E-5
66-67	0.0023293537646031483	3.072587853487219E-4	3.3217165983645615E-5	1.4532510117844956E-5	0.12307998033377687	1.6608582991822807E-5
68-69	0.0023708752220827057	3.155630768446333E-4	3.3217165983645615E-5	1.6608582991822807E-5	0.12427164616344016	2.0760728739778507E-5
70-71	0.002391635950822484	3.2386736834054473E-4	3.3217165983645615E-5	1.6608582991822807E-5	0.12544462733723766	2.0760728739778507E-5
72-73	0.0024310813354280632	3.404759513323675E-4	3.3217165983645615E-5	1.6608582991822807E-5	0.12636847976615778	2.4912874487734208E-5
74-75	0.0024539181370418195	3.4255202420634535E-4	3.529323885762346E-5	1.6608582991822807E-5	0.12735046223554933	2.4912874487734208E-5
76-77	0.0024788310115295537	3.550084614502125E-4	4.1521457479557015E-5	1.6608582991822807E-5	0.128506834826355	2.4912874487734208E-5
78-79	0.002526580687631044	3.736931173160131E-4	4.1521457479557015E-5	1.8684655865800657E-5	0.1300638894818384	2.698894736171206E-5
80-81	0.0025369610520009334	3.840734816859024E-4	5.813004047137982E-5	2.2836801613756358E-5	0.1314382497244117	3.114109310966776E-5
82-83	0.002545265343496845	4.1936672054352584E-4	5.813004047137982E-5	2.4912874487734208E-5	0.13309080373209808	3.3217165983645615E-5
84-85	0.002574330363732535	4.2351886629148154E-4	5.813004047137982E-5	2.4912874487734208E-5	0.13460010871147998	3.529323885762346E-5
86-87	0.0025971671653462914	4.4012744928330436E-4	5.813004047137982E-5	2.906502023568991E-5	0.13637307494585704	3.7369311731601315E-5
88-89	0.0026096236025901585	4.546599594011493E-4	6.228218621933552E-5	2.906502023568991E-5	0.13799656393330773	3.7369311731601315E-5
90-91	0.0026241561127080034	4.6919246951899427E-4	6.228218621933552E-5	3.3217165983645615E-5	0.1388913513419922	3.7369311731601315E-5
92-93	0.002630384331329937	4.712685423929721E-4	6.228218621933552E-5	3.7369311731601315E-5	0.1397716062405588	4.1521457479557015E-5
94-95	0.0026366125499518705	4.7749676101490566E-4	6.228218621933552E-5	3.7369311731601315E-5	0.14065393721199937	4.1521457479557015E-5
96-97	0.002655297205817671	4.837249796368392E-4	6.228218621933552E-5	3.7369311731601315E-5	0.14152588781907008	4.5673603227512715E-5
98-99	0.002659449351565627	5.00333562628662E-4	6.643433196729123E-5	3.7369311731601315E-5	0.14244558810224228	5.190182184944626E-5
100-101	0.002669829715935516	5.024096355026399E-4	7.058647771524692E-5	3.7369311731601315E-5	0.14311408356766314	5.3977894723424115E-5
102-103	0.0026739818616834717	5.086378541245734E-4	7.473862346320263E-5	3.7369311731601315E-5	0.14393828449863233	6.228218621933552E-5
104-105	0.0026905904446752945	5.14866072746507E-4	7.473862346320263E-5	3.7369311731601315E-5	0.14478532223121532	6.228218621933552E-5
106-107	0.002721731537784962	5.314746557383298E-4	7.473862346320263E-5	3.7369311731601315E-5	0.14547665449824992	6.643433196729123E-5
108-109	0.0027279597564068957	5.418550201082191E-4	7.473862346320263E-5	3.944538460557917E-5	0.14627386648185742	7.266255058922477E-5
110-111	0.002763252995264519	5.688439674699311E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14709806741282663	7.681469633718048E-5
112-113	0.002775709432508386	5.792243318398203E-4	7.473862346320263E-5	4.1521457479557015E-5	0.1478703665219464	7.889076921115832E-5
114-115	0.0027819376511303198	5.813004047137982E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14877968644074868	8.096684208513618E-5
116-117	0.0027840137240042976	5.854525504617538E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14965578919356734	8.304291495911403E-5
118-119	0.0027881658697522533	5.999850605795989E-4	7.473862346320263E-5	4.359753035353486E-5	0.15038656684520757	8.304291495911403E-5
120-121	0.002792318015500209	6.062132792015324E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15111942056972172	8.719506070706972E-5
122-123	0.002796470161248165	6.103654249494881E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15177130745215078	8.927113358104757E-5
124-125	0.0028026983798700987	6.186697164453995E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15259966052886795	9.342327932900329E-5
126-127	0.0028172308899879436	6.228218621933552E-4	7.473862346320263E-5	4.5673603227512715E-5	0.1535214368849141	1.0172757082491468E-4
128-129	0.002837991618727722	6.332022265632444E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15439131141911083	1.0795578944684823E-4
130-131	0.0028483719830976113	6.415065180591559E-4	7.473862346320263E-5	4.5673603227512715E-5	0.1552757184634254	1.1003186232082609E-4
132-133	0.002864980566089434	6.435825909331338E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15608538688427676	1.1210793519480394E-4
134-135	0.002891969513451146	6.498108095550673E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15680370809867308	1.1626008094275964E-4
136-137	0.0028961216591991018	6.601911739249565E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15750749680295156	1.1626008094275964E-4
138-139	0.0028981977320730796	6.80951902664735E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15813862295664083	1.2456437243867104E-4
>>END_MODULE
