##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24135576
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.10786678552855	37.0	35.0	38.0	33.0	39.0
2	35.486142738006336	35.0	34.0	38.0	33.0	39.0
3	35.60446578942222	35.0	35.0	38.0	33.0	39.0
4	35.67654503045629	36.0	35.0	38.0	33.0	39.0
5	35.599269849619496	36.0	35.0	38.0	33.0	39.0
6	35.54016962346372	35.0	35.0	37.0	33.0	39.0
7	35.56966819437	35.0	34.0	38.0	33.0	39.0
8	35.67667077843927	36.0	35.0	38.0	33.0	39.0
9	35.65715166690035	36.0	35.0	38.0	33.0	39.0
10-14	35.63672550429292	35.8	35.0	37.8	33.0	39.0
15-19	35.61399854720683	36.0	35.0	38.0	33.0	39.0
20-24	35.60146779177758	36.0	35.0	38.0	33.0	39.0
25-29	35.584040273163566	36.0	35.0	38.0	33.0	39.0
30-34	35.567473392804054	36.0	35.0	38.0	33.0	39.0
35-39	35.53822202544493	36.0	35.0	38.0	33.0	39.0
40-44	35.52381258272021	36.0	35.0	38.0	33.0	39.0
45-49	35.49289768762925	36.0	35.0	38.0	33.0	39.0
50-54	35.47044347315349	36.0	34.6	38.0	33.0	39.0
55-59	35.44588168933693	36.0	34.2	38.0	33.0	39.0
60-64	35.40383676776556	36.0	34.0	38.0	33.0	39.0
65-69	35.37504388682599	36.0	34.0	38.0	32.6	39.0
70-74	35.341634000240994	36.0	34.0	38.0	32.0	39.0
75-79	35.29243109775011	36.0	34.0	38.0	32.0	39.0
80-84	35.249933366003184	36.0	34.0	38.0	32.0	39.0
85-89	35.19785554898778	36.0	34.0	38.0	32.0	39.0
90-94	35.14362316218052	36.0	34.0	38.0	32.0	39.0
95-99	35.07381846850095	36.0	34.0	38.0	32.0	39.0
100-104	35.02537986089895	36.0	34.0	38.0	32.0	39.0
105-109	34.96430187486949	35.4	34.0	37.8	31.2	39.0
110-114	34.88532557107845	35.0	34.0	37.2	31.0	39.0
115-119	34.81140004293466	35.0	34.0	37.0	31.0	39.0
120-124	34.734614907521994	35.0	34.0	37.0	31.0	39.0
125-129	34.65157465782651	35.0	34.0	37.0	30.0	39.0
130-134	34.5643234135203	35.0	34.0	37.0	30.0	39.0
135-139	34.48765492483481	35.0	34.0	37.0	30.0	39.0
140-144	34.36256722998143	35.0	34.0	37.0	29.8	39.0
145-149	34.34740247875821	35.0	34.0	37.0	29.4	39.0
150	35.105473568264614	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	114.0
22	1171.0
23	6521.0
24	23673.0
25	60395.0
26	123171.0
27	217907.0
28	326791.0
29	442477.0
30	580945.0
31	771914.0
32	1052820.0
33	1497153.0
34	2281585.0
35	3932517.0
36	1.0475275E7
37	2341135.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.77121805586906	18.728875581838196	15.397241814324216	32.10266454796852
2	24.555800947116406	25.475103639540237	32.114829163389345	17.85426624995401
3	20.455733063921905	29.037931392231947	29.31016438140942	21.196171162436727
4	23.772612677650617	33.8076207503811	21.37794432583668	21.041822246131602
5	25.87979255187446	34.78134103781074	21.45858462213622	17.88028178817858
6	19.570492123328652	38.76712948553621	21.769035054311527	19.893343336823616
7	20.871617068513302	20.653151182304494	36.47683403122428	21.99839771795792
8	21.947704914935528	23.399822734704983	27.844079627517488	26.808392722842
9	23.77501162599144	23.25808590605006	27.58329446954156	25.38360799841694
10-14	23.41690540138756	28.618427834496263	25.032011666098214	22.93265509801796
15-19	23.53247421979902	27.598909593042237	26.439263765654488	22.429352421504255
20-24	23.365146122885154	27.651730375111	26.36202840155959	22.621095100444258
25-29	23.503724957713874	27.297169124946514	26.3066777440903	22.892428173249314
30-34	23.2776404424738	27.03633756244309	26.5309110501444	23.15511094493871
35-39	23.61407594841802	26.814799255794014	26.538478522646226	23.03264627314174
40-44	23.784952967354084	26.489609363372974	26.56564152436221	23.159796144910732
45-49	23.69400028981285	26.342314982184927	26.691608492721354	23.272076235280867
50-54	23.84663950013043	26.32642204188539	26.46011597154342	23.366822486440764
55-59	23.90365574867573	26.299237275298502	26.541167279372157	23.255939696653606
60-64	23.55065816535723	26.263066603423923	26.62556634239846	23.560708888820386
65-69	23.748867283987828	26.1964453878928	26.638369845207322	23.41631748291205
70-74	23.939388867035476	26.192095101478042	26.342936395193718	23.525579636292765
75-79	23.77274076131835	26.2813566209968	26.61547125554047	23.330431362144385
80-84	24.107936752271122	26.244182453813842	26.3206282909277	23.32725250298734
85-89	23.809872279210754	26.37575915271555	26.547535451986416	23.266833116087284
90-94	23.823666691322035	26.124027520960226	26.57197905673641	23.480326730981325
95-99	23.784833668801088	26.370443742058402	26.654202908047925	23.190519681092585
100-104	24.079295541944383	26.08021647656566	26.587805303843915	23.252682677646042
105-109	23.842782024233884	26.295352434771647	26.556114295588344	23.305751245406128
110-114	23.95881237551291	26.364079749467685	26.47140646735584	23.205701407663568
115-119	24.04453869196748	26.425941353101212	26.63205898548912	22.897460969442186
120-124	23.998529458830937	26.59775207949461	26.756231713227034	22.647486748447413
125-129	23.997609679137042	26.63029665311653	26.60224874539398	22.769844922352444
130-134	24.167348109315625	26.628148177394834	26.6147501393115	22.589753573978044
135-139	23.959311049362828	26.68829207612576	26.837532151101296	22.51486472341012
140-144	23.96797826947095	26.839467086761566	26.660506149174353	22.532048494593138
145-149	23.733212595022312	27.129339124913663	26.47219145465937	22.665256825404654
150	23.89569721579264	25.94603436897449	26.730601698607593	23.42766671662527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.5
9	0.5
10	0.0
11	1.5
12	7.0
13	11.0
14	18.0
15	25.5
16	47.5
17	94.0
18	166.0
19	272.0
20	416.0
21	645.0
22	1018.0
23	1678.0
24	2667.0
25	4341.0
26	6886.5
27	10685.5
28	16340.5
29	23384.5
30	33018.5
31	47185.0
32	67374.5
33	92394.5
34	124568.0
35	175630.5
36	249299.5
37	337558.5
38	447678.5
39	587245.5
40	752200.0
41	936225.0
42	1131392.0
43	1315402.0
44	1461722.5
45	1563042.0
46	1603398.0
47	1582764.0
48	1547253.5
49	1514238.5
50	1418430.5
51	1261084.0
52	1088711.0
53	917886.5
54	768733.5
55	651892.0
56	550842.5
57	461250.5
58	388259.0
59	322143.5
60	249852.5
61	170282.5
62	103086.0
63	60951.5
64	37101.5
65	19019.0
66	10942.0
67	6036.5
68	3116.5
69	1894.0
70	1300.0
71	1187.0
72	1096.0
73	893.5
74	725.5
75	420.0
76	127.0
77	43.0
78	13.0
79	1.5
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.286522766226917E-7
40-44	0.0
45-49	8.286522766226917E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.286523727557463E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	8.287787932878E-7
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	2.0
66-67	2.0
68-69	1.0
70-71	4.0
72-73	7.0
74-75	9.0
76-77	5.0
78-79	13.0
80-81	15.0
82-83	12.0
84-85	31.0
86-87	30.0
88-89	34.0
90-91	30.0
92-93	40.0
94-95	49.0
96-97	70.0
98-99	79.0
100-101	103.0
102-103	82.0
104-105	99.0
106-107	77.0
108-109	123.0
110-111	127.0
112-113	128.0
114-115	155.0
116-117	140.0
118-119	176.0
120-121	227.0
122-123	254.0
124-125	285.0
126-127	342.0
128-129	408.0
130-131	482.0
132-133	642.0
134-135	835.0
136-137	1503.0
138-139	5116.0
140-141	9292.0
142-143	9662.0
144-145	59312.0
146-147	788427.0
148-149	1005391.0
150-151	2.2251755E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.13380492348481
#Duplication Level	Percentage of total
1	25.975151434963113
2	8.241274200208434
3	5.1178091602318645
4	3.5152413746368203
5	2.7455550707219096
6	2.167876176619638
7	1.7151771336230173
8	1.4430878892600507
9	1.2044388075316177
>10	15.465241467638483
>50	5.711479177112234
>100	13.522447343528402
>500	5.128762846693574
>1k	6.773093735726018
>5k	0.7728218142486395
>10k+	0.5005423672557794
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.9775309277889203E-4	8.286522766226917E-6	0.0	0.0	0.0021462093964527717	0.0
2	0.0018106052244205814	2.9002829681794213E-5	0.0	0.0	0.009508784874245387	0.0
3	0.0026889766376406348	2.9002829681794213E-5	0.0	0.0	0.014116091532267554	0.0
4	0.006347476438929819	2.9002829681794213E-5	0.0	0.0	0.02125078763398893	0.0
5	0.006550496246702378	2.9002829681794213E-5	0.0	0.0	0.026206128248192625	0.0
6	0.0076898931270585795	2.9002829681794213E-5	0.0	0.0	0.03348169523693986	0.0
7	0.009910681228407393	3.314609106490767E-5	8.286522766226917E-6	0.0	0.04652468207098103	0.0
8	0.017194534739920855	3.728935244802113E-5	8.286522766226917E-6	0.0	0.06335046654780478	0.0
9	0.017787021117706078	4.143261383113459E-5	8.286522766226917E-6	0.0	0.07598327050491772	0.0
10-11	0.01865503437746835	4.143261383113459E-5	8.286522766226917E-6	0.0	0.12431855780031933	0.0
12-13	0.01958105329659421	4.9719136597361504E-5	8.286522766226917E-6	0.0	0.1488528800804257	0.0
14-15	0.022872874465477852	5.3862397980474965E-5	8.286522766226917E-6	4.143261383113458E-6	0.1585750429159014	0.0
16-17	0.02646301045394566	5.3862397980474965E-5	8.286522766226917E-6	4.143261383113458E-6	0.16547978801086	0.0
18-19	0.0332165265084206	6.007729005514515E-5	8.286522766226917E-6	4.143261383113458E-6	0.17198263675165656	0.0
20-21	0.03937134129303564	6.836381282137207E-5	8.286522766226917E-6	4.143261383113458E-6	0.17637242218706528	0.0
22-23	0.0418055073556148	7.872196627915571E-5	8.286522766226917E-6	4.143261383113458E-6	0.1812096798518502	0.0
24-25	0.04747141729702245	8.079359697071244E-5	8.286522766226917E-6	4.143261383113458E-6	0.18577969715742437	0.0
26-27	0.05241432812707681	8.700848904538263E-5	8.286522766226917E-6	4.143261383113458E-6	0.1892931828103046	0.0
28-29	0.06483996901503407	9.115175042849609E-5	8.286522766226917E-6	4.143261383113458E-6	0.1926865138830745	0.0
30-31	0.08881287937772854	9.115175042849609E-5	8.286522766226917E-6	6.214892074670188E-6	0.19616892507558137	0.0
32-33	0.10790709946180692	1.0150990388627974E-4	8.286522766226917E-6	8.286522766226917E-6	0.19900498749232254	0.0
34-35	0.12160264996368846	1.0358153457783648E-4	8.286522766226917E-6	8.286522766226917E-6	0.20200678036438824	0.0
36-37	0.12384001111056972	1.0772479596094993E-4	8.286522766226917E-6	8.286522766226917E-6	0.2039561848451431	4.143261383113458E-6
38-39	0.1259945070297887	1.1186805734406338E-4	8.286522766226917E-6	8.286522766226917E-6	0.20548711992620355	4.143261383113458E-6
40-41	0.1275275137415407	1.1601131872717685E-4	8.286522766226917E-6	8.286522766226917E-6	0.20687096922816345	4.143261383113458E-6
42-43	0.1278341150838911	1.1808294941873358E-4	8.286522766226917E-6	8.286522766226917E-6	0.20836461495677583	4.143261383113458E-6
44-45	0.12868555529812092	1.2844110287651723E-4	8.286522766226917E-6	8.286522766226917E-6	0.20958480543410274	4.143261383113458E-6
46-47	0.12999689752587631	1.2844110287651723E-4	8.286522766226917E-6	8.286522766226917E-6	0.2108940760311666	4.143261383113458E-6
48-49	0.1312191596338948	1.3051273356807396E-4	8.286522766226917E-6	8.286522766226917E-6	0.21196925236008454	4.143261383113458E-6
50-51	0.13212031898472198	1.3258436425963067E-4	8.286522766226917E-6	8.286522766226917E-6	0.21304442868900247	4.143261383113458E-6
52-53	0.13297383082964334	1.3258436425963067E-4	8.286522766226917E-6	8.286522766226917E-6	0.214140321324836	4.143261383113458E-6
54-55	0.13337158392242224	1.491574097920845E-4	8.286522766226917E-6	8.286522766226917E-6	0.21501454947667292	4.143261383113458E-6
56-57	0.1340531504199444	1.491574097920845E-4	8.286522766226917E-6	8.286522766226917E-6	0.2160793676521331	4.143261383113458E-6
58-59	0.13421888087526895	1.5122904048364125E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21698259863365182	6.214892074670188E-6
60-61	0.13452962547900243	1.5330067117519798E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21763723393218376	8.286522766226917E-6
62-63	0.13472228713331724	1.615871939414249E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21839959402667664	8.286522766226917E-6
64-65	0.13476579137783992	1.6365882463298162E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21908944704696504	8.286522766226917E-6
66-67	0.13483622682135285	1.698737167076518E-4	8.286522766226917E-6	1.2429784149340376E-5	0.2197130078851236	8.286522766226917E-6
68-69	0.13491287715694045	1.8644676224010564E-4	8.286522766226917E-6	1.2429784149340376E-5	0.22040493253610355	1.0358153457783645E-5
70-71	0.13501853032220984	1.8644676224010564E-4	8.286522766226917E-6	1.6573045532453833E-5	0.2211548628464471	1.2429784149340376E-5
72-73	0.13508067924295653	1.8644676224010564E-4	8.286522766226917E-6	1.6573045532453833E-5	0.22166034073518692	1.2429784149340376E-5
74-75	0.13509518065779744	1.8644676224010564E-4	8.286522766226917E-6	2.0716306915567294E-5	0.22229011646542018	1.2429784149340376E-5
76-77	0.13514075653301166	1.9059002362321908E-4	8.286522766226917E-6	2.0716306915567294E-5	0.22317263114002334	1.4501414840897105E-5
78-79	0.13518011751615128	2.0301980777255948E-4	8.286522766226917E-6	2.0716306915567294E-5	0.2242374493154835	1.6573045532453833E-5
80-81	0.13524019480620642	2.0509143846411622E-4	8.286522766226917E-6	2.0716306915567294E-5	0.2249210874436972	1.6573045532453833E-5
82-83	0.1352733408972713	2.1544959192189986E-4	1.2429784149340376E-5	2.0716306915567294E-5	0.22568344753819009	1.6573045532453833E-5
84-85	0.13531477351110244	2.2166448399657003E-4	1.4501414840897105E-5	2.0716306915567294E-5	0.22658253525832572	1.6573045532453833E-5
86-87	0.13534377634078423	2.258077453796835E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22740497264287374	2.0716306915567294E-5
88-89	0.13537277917046603	2.2787937607124023E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22819012067497374	2.0716306915567294E-5
90-91	0.1353934954773816	2.3616589883746714E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22874946096169407	2.4859568298680752E-5
92-93	0.1354142117842972	2.465240522952508E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22929429983357347	2.4859568298680752E-5
94-95	0.13545357276743675	2.52738944369921E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.23004837340530013	3.107446037335094E-5
96-97	0.135461859290203	2.52738944369921E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.23075687110181253	3.314609106490767E-5
98-99	0.1354887904891932	2.589538364445912E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.2313431425875231	3.314609106490767E-5
100-101	0.13552193658025813	2.610254671361479E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23186519352179538	3.314609106490767E-5
102-103	0.13554058125648213	2.630970978277046E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23237067141053522	3.314609106490767E-5
104-105	0.13557165571685548	2.6516872851926134E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.2329217251744893	3.314609106490767E-5
106-107	0.1356048018079204	2.7138362059393154E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23341684490977138	3.314609106490767E-5
108-109	0.13562966137621907	2.75526881977045E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.2339471823668099	3.314609106490767E-5
110-111	0.1356710939900502	2.7759851266860174E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23455831342081912	3.314609106490767E-5
112-113	0.1356897386662742	2.7967014336015847E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.2351632295827537	3.314609106490767E-5
114-115	0.135700096819732	2.817417740517152E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23582615140405183	3.314609106490767E-5
116-117	0.13573117128010537	2.817417740517152E-4	2.6931198990237482E-5	2.0716306915567294E-5	0.23632541440071703	3.5217721756464394E-5
118-119	0.1357477443256378	2.900282968179421E-4	2.9002829681794213E-5	2.0716306915567294E-5	0.23683503555083996	3.728935244802113E-5
120-121	0.13576431737117026	2.9417155820105555E-4	2.9002829681794213E-5	2.0716306915567294E-5	0.23756424955426791	3.936098313957786E-5
122-123	0.1357850336780858	2.9417155820105555E-4	2.9002829681794213E-5	2.0716306915567294E-5	0.23806765581231623	4.143261383113459E-5
124-125	0.13579332020085205	2.9417155820105555E-4	3.314609106490767E-5	2.0716306915567294E-5	0.2386725719742508	4.143261383113459E-5
126-127	0.13580782161569294	2.9417155820105555E-4	3.314609106490767E-5	2.0716306915567294E-5	0.2393044193351756	4.557587521424804E-5
128-129	0.13581196487707606	2.98314819584169E-4	3.314609106490767E-5	2.0716306915567294E-5	0.23994869648024975	4.557587521424804E-5
130-131	0.13582025139984227	3.148878651166229E-4	3.314609106490767E-5	2.0716306915567294E-5	0.240779420387564	4.557587521424804E-5
132-133	0.13583682444537476	3.190311264997363E-4	3.314609106490767E-5	2.0716306915567294E-5	0.24147963156131014	4.557587521424804E-5
134-135	0.13587204216713122	3.190311264997363E-4	3.728935244802113E-5	2.0716306915567294E-5	0.2420907626153194	4.557587521424804E-5
136-137	0.13589483010473832	3.2110275719129304E-4	3.728935244802113E-5	2.0716306915567294E-5	0.24265838942480594	4.764750590580477E-5
138-139	0.13589483010473832	3.2938927995752E-4	3.728935244802113E-5	2.0716306915567294E-5	0.2432550190639743	4.9719136597361504E-5
>>END_MODULE
