##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.2.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24190877
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	70-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.454808562748674	36.0	34.0	38.0	31.0	39.0
2	34.75655892921947	35.0	34.0	38.0	31.0	39.0
3	34.941786070839846	35.0	34.0	38.0	31.0	39.0
4	34.89190036392645	36.0	34.0	38.0	31.0	39.0
5	34.75618552398907	35.0	34.0	37.0	31.0	39.0
6	34.72844436355077	35.0	34.0	37.0	31.0	39.0
7	34.74392197521404	35.0	34.0	37.0	32.0	39.0
8	34.885087795700834	35.0	34.0	37.0	31.0	39.0
9	34.867721207461805	35.0	34.0	37.0	31.0	39.0
10-14	34.848934414407545	35.0	34.0	37.4	31.0	39.0
15-19	34.84694017500895	35.0	34.0	37.0	31.0	39.0
20-24	34.85276699145715	35.0	34.0	37.0	31.0	39.0
25-29	34.84789581626164	35.0	34.0	37.0	31.0	39.0
30-34	34.82954407151093	35.0	34.0	37.0	31.0	39.0
35-39	34.82257630428198	35.0	34.0	37.0	31.0	39.0
40-44	34.756094192037764	35.0	34.0	37.0	31.0	39.0
45-49	34.65496633296924	35.0	34.0	37.0	31.0	39.0
50-54	34.61905407563356	35.0	34.0	37.0	30.6	39.0
55-59	34.61030836542222	35.0	34.0	37.0	30.6	39.0
60-64	34.58047997185055	35.0	34.0	37.0	30.0	39.0
65-69	34.56325787609933	35.0	34.0	37.0	30.0	39.0
70-74	34.53720053338688	35.0	34.0	37.0	30.0	39.0
75-79	34.51646466569191	35.0	34.0	37.0	30.0	39.0
80-84	34.48754560219344	35.0	34.0	37.0	30.0	39.0
85-89	34.47062557002926	35.0	34.0	37.0	30.0	39.0
90-94	34.44313039202898	35.0	34.0	37.0	30.0	39.0
95-99	34.40769052117956	35.0	34.0	37.0	30.0	39.0
100-104	34.37010291812747	35.0	34.0	37.0	30.0	39.0
105-109	34.31363782404923	35.0	34.0	37.0	30.0	39.0
110-114	34.28650074238371	35.0	34.0	37.0	30.0	39.0
115-119	34.23664348965643	35.0	34.0	37.0	29.6	39.0
120-124	34.199900777957225	35.0	34.0	37.0	29.0	39.0
125-129	34.14636704582876	35.0	34.0	37.0	29.0	39.0
130-134	34.10194883652282	35.0	34.0	37.0	29.0	39.0
135-139	34.05140404832432	35.0	34.0	37.0	29.0	39.0
140-144	34.02748131190812	35.0	34.0	37.0	29.0	39.0
145-149	34.03513867034757	35.0	34.0	37.0	29.0	39.0
150	34.96596852667538	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	18.0
22	293.0
23	3517.0
24	23621.0
25	92355.0
26	226680.0
27	402142.0
28	583455.0
29	755357.0
30	943432.0
31	1176897.0
32	1494995.0
33	1951169.0
34	2666759.0
35	4081952.0
36	8272456.0
37	1515771.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.414963376482795	10.888133572007332	14.623566561890252	38.07333648961962
2	22.837914329814797	12.569934003540101	36.13265564990852	28.45949601673658
3	21.675633759016165	16.53240589761612	27.236589655621575	34.55537068774615
4	26.968735762963767	24.246257895593406	21.76382929971051	27.02117704173232
5	27.024755005660197	29.805354211186742	23.845848422534704	19.324042360618357
6	20.183475126591233	35.88767887235399	25.46140785273641	18.46743814831837
7	17.067024378044156	24.287691717831038	39.09538016996113	19.549903734163678
8	19.417035780057333	24.174437855635496	30.790472430691768	25.618053933615396
9	20.1315034349143	22.99940998355122	32.036467009779855	24.832619571754627
10-14	21.22008738761914	28.36431027759638	26.497960943152	23.917641391632475
15-19	21.429559702196702	27.435671096532865	27.137371908296394	23.997397292974036
20-24	21.41048069207106	27.405375336835224	27.047086735803212	24.137057235290502
25-29	21.33257313252962	27.638735440984608	26.725551142757926	24.30314028372785
30-34	21.36021392571846	27.435442774623937	26.898344954146218	24.305998345511387
35-39	21.599704454876118	27.152857708671075	26.852279243115714	24.395158593337097
40-44	21.79949516516811	27.206381263606342	26.655561712481195	24.338561858744352
45-49	22.030977651916555	27.184172903059924	26.59044056870698	24.194408876316544
50-54	22.006136114670024	27.40180570030672	26.475349827347628	24.116708357675627
55-59	21.957019807075092	27.30921014279647	26.598139236534422	24.135630813594016
60-64	22.175983282781235	27.159985022747186	26.345493349208006	24.318538345263576
65-69	22.04652716946579	27.25907257184416	26.526561962192307	24.167838296497738
70-74	22.39772837231907	26.995408567635675	26.471596105282224	24.135266954763026
75-79	22.225133612734226	26.927744621014725	26.441286293537615	24.405835472713438
80-84	22.48802196777014	26.931332959482983	26.46468336157494	24.115961711171934
85-89	22.338909072654165	26.915875690097014	26.49045238371731	24.25476285353151
90-94	22.32423828716169	26.676222138131063	26.48537093503539	24.51416863967186
95-99	22.569916823885304	26.726274502748847	26.655866228097825	24.047942445268028
100-104	22.532012030763113	26.534254700703773	26.67252495594109	24.26120831259202
105-109	22.659398788770062	26.516614069115786	26.562760563578706	24.261226578535446
110-114	22.56326978289249	26.55874712140434	26.571771414194874	24.306211681508294
115-119	22.67745589199063	26.797363421892996	26.424307829788756	24.10087285632762
120-124	22.590884211779567	26.64153721752417	26.502352242774606	24.26522632792166
125-129	23.002574572245095	26.59364335272582	26.403591246545314	24.000190828483763
130-134	22.672036822184833	26.856435915765825	26.29425170466203	24.17727555738731
135-139	22.811992048515425	26.609713766582566	26.373896812467727	24.204397372434283
140-144	22.750792477622834	26.471474958183595	26.66834155091513	24.109391013278444
145-149	22.216234911874643	26.54670913847086	27.02116936091557	24.21588658873893
150	21.975888409624872	25.15511830643543	27.734536543476345	25.13445674046335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	1.0
10	1.0
11	3.0
12	10.5
13	16.0
14	24.5
15	60.0
16	107.5
17	232.0
18	544.5
19	974.0
20	1578.0
21	2454.5
22	4112.0
23	6768.5
24	10850.0
25	16346.0
26	22814.0
27	32303.5
28	49029.5
29	72573.0
30	99128.5
31	125569.5
32	152810.5
33	185552.0
34	220168.5
35	265076.0
36	331958.5
37	405815.0
38	477708.0
39	578860.0
40	720434.0
41	877976.5
42	1039390.0
43	1194082.0
44	1330639.5
45	1435486.5
46	1514299.0
47	1545080.0
48	1502325.0
49	1452982.5
50	1378494.0
51	1240821.0
52	1068488.5
53	918830.5
54	778413.0
55	651652.0
56	566393.5
57	473861.0
58	394846.0
59	329970.0
60	260678.0
61	184156.5
62	107586.5
63	60431.5
64	39002.5
65	24851.5
66	16652.0
67	8000.5
68	3477.5
69	2112.0
70	1616.0
71	1390.0
72	1280.5
73	957.0
74	531.0
75	172.5
76	41.0
77	25.0
78	11.5
79	2.5
80	2.5
81	3.0
82	1.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.405488523628143E-4
3	7.440821595678404E-5
4	4.133789775376891E-5
5	4.96054773045227E-5
6	9.50771648336685E-5
7	2.1495706831959832E-4
8	1.1161232393517606E-4
9	1.0747853415979916E-4
10-14	1.4881643191356808E-4
15-19	2.6290902971397024E-4
20-24	1.785797182962817E-4
25-29	3.1251450701849296E-4
30-34	8.259311971203028E-4
35-39	7.796327516360817E-4
40-44	9.606927437975896E-4
45-49	8.763634323799008E-4
50-54	5.357391548888452E-4
55-59	4.0015085025648305E-4
60-64	2.4306683879216117E-4
65-69	3.050736854228146E-4
70-74	3.348369828786937E-4
75-79	2.546415280581728E-4
80-84	2.7283042066465887E-4
85-89	3.530265574438324E-4
90-94	3.8609765044532647E-4
95-99	2.91847775573459E-4
100-104	4.6795103019791343E-4
105-109	2.943313388085907E-4
110-114	2.5134101801330486E-4
115-119	2.2405939349867112E-4
120-124	2.000849253024271E-4
125-129	1.289826909445494E-4
130-134	1.4469636117673596E-4
135-139	1.4635952027086799E-4
140-144	1.5551566413328342E-4
145-149	1.6946514893566867E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
70-71	1.0
72-73	0.0
74-75	4.0
76-77	5.0
78-79	6.0
80-81	9.0
82-83	14.0
84-85	18.0
86-87	14.0
88-89	9.0
90-91	23.0
92-93	34.0
94-95	30.0
96-97	30.0
98-99	49.0
100-101	70.0
102-103	50.0
104-105	63.0
106-107	49.0
108-109	72.0
110-111	80.0
112-113	98.0
114-115	82.0
116-117	104.0
118-119	101.0
120-121	123.0
122-123	155.0
124-125	179.0
126-127	211.0
128-129	278.0
130-131	297.0
132-133	421.0
134-135	570.0
136-137	1028.0
138-139	2324.0
140-141	5976.0
142-143	9233.0
144-145	72172.0
146-147	772337.0
148-149	1448191.0
150-151	2.1876367E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.05007151459901
#Duplication Level	Percentage of total
1	32.86882196263382
2	8.51280024873629
3	5.051748821379286
4	3.4067378421513146
5	2.6253202823424404
6	2.098710402901564
7	1.6728777621057633
8	1.4275845967346994
9	1.2110145472009355
>10	15.378013001122234
>50	5.185309413486159
>100	11.3655126427181
>500	4.260545960846543
>1k	4.541315700812882
>5k	0.33516942662574134
>10k+	0.05851738820243342
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.6535159101507563E-5	0.0	8.267579550753781E-6	2.769639149502517E-4	2.0668948876884454E-5
2	2.8936528427638237E-5	5.3739267079899587E-5	0.0	1.6535159101507563E-5	0.0023645277515155815	4.5471687529145804E-5
3	5.7873056855276475E-5	6.200684663065336E-5	0.0	1.6535159101507563E-5	0.004555436332465334	5.7873056855276475E-5
4	1.2814748303668363E-4	7.027442618140715E-5	0.0	2.0668948876884454E-5	0.006771147652067348	6.200684663065336E-5
5	1.364150625874374E-4	7.027442618140715E-5	0.0	2.4802738652261346E-5	0.007953411527825138	6.614063640603025E-5
6	1.9842190921809077E-4	7.027442618140715E-5	0.0	2.4802738652261346E-5	0.00971853976191107	6.614063640603025E-5
7	2.4802738652261345E-4	8.68095852829147E-5	0.0	2.4802738652261346E-5	0.011479534206221626	7.440821595678404E-5
8	3.761748695592971E-4	8.68095852829147E-5	0.0	2.4802738652261346E-5	0.015195811214285452	8.267579550753782E-5
9	4.3818171618995044E-4	8.68095852829147E-5	0.0	2.4802738652261346E-5	0.01823001290941209	8.68095852829147E-5
10-11	5.270581963605536E-4	1.0747853415979917E-4	0.0	2.6869633539949793E-5	0.0312328486478601	1.0334474438442227E-4
12-13	5.580616196758803E-4	1.1161232393517606E-4	0.0	3.100342331532668E-5	0.03980012795732871	1.2608058814899518E-4
14-15	6.63473258947991E-4	1.1574611371055295E-4	0.0	3.3070318203015126E-5	0.04641005780815635	1.2814748303668363E-4
16-17	7.709517931077901E-4	1.1987990348592984E-4	0.0	3.3070318203015126E-5	0.050578984796623955	1.322812728120605E-4
18-19	9.280358045721121E-4	1.2401369326130673E-4	0.0	3.3070318203015126E-5	0.05364005612529054	1.3434816769974895E-4
20-21	0.001345548571885178	1.2814748303668363E-4	0.0	3.3070318203015126E-5	0.056134798254730486	1.364150625874374E-4
22-23	0.0017031213874552792	1.2814748303668363E-4	0.0	3.3070318203015126E-5	0.0588176278189501	1.405488523628143E-4
24-25	0.001837469555155028	1.322812728120605E-4	0.0	3.720410797839202E-5	0.061533527701372714	1.4261574725050276E-4
26-27	0.0019325467199886965	1.322812728120605E-4	0.0	4.5471687529145804E-5	0.06367896459479332	1.6121780123969873E-4
28-29	0.0022074437400512598	1.322812728120605E-4	0.0	5.3739267079899587E-5	0.06653127953980337	1.6535159101507563E-4
30-31	0.0026993647233211097	1.322812728120605E-4	0.0	7.027442618140715E-5	0.0694600695956579	1.6948538079045254E-4
32-33	0.0030155996411374416	1.364150625874374E-4	0.0	7.234132106909559E-5	0.07207055783880842	1.8188675011658321E-4
34-35	0.0032388242890077938	1.364150625874374E-4	0.0	8.680958528291472E-5	0.07476372187746644	1.8808743477964854E-4
36-37	0.0032760283969861864	1.364150625874374E-4	0.0	1.0127784949673382E-4	0.07731840395864936	1.9635501433040232E-4
38-39	0.0033070318203015127	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.07984208261651696	2.0668948876884454E-4
40-41	0.0033421690333922164	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.0821611386805034	2.1495706831959835E-4
42-43	0.0033628379822691007	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.08460420843775114	2.2322464787035212E-4
44-45	0.0033979751953598044	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.08727050284286923	2.27358437645729E-4
46-47	0.0034496475675520155	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.09116866660104964	2.314922274211059E-4
48-49	0.003474450306204277	1.3848195747512585E-4	0.0	1.322812728120605E-4	0.09379155621352628	2.356260171964828E-4
50-51	0.003513721309070357	1.508833268012565E-4	0.0	1.364150625874374E-4	0.09635657276914764	2.356260171964828E-4
52-53	0.0035653936812625685	1.550171165766334E-4	0.0	1.364150625874374E-4	0.09864669230470643	2.3975980697185968E-4
54-55	0.0036005308943532722	1.5708401146432186E-4	0.0	1.364150625874374E-4	0.10033947921772328	2.3975980697185968E-4
56-57	0.0036253336330055335	1.5708401146432186E-4	0.0	1.4261574725050276E-4	0.10230302936102731	2.4596049163492503E-4
58-59	0.0036666715307593026	1.6741848590276409E-4	0.0	1.4881643191356808E-4	0.10427484708388207	2.4802738652261345E-4
60-61	0.00374934732626684	1.6948538079045254E-4	0.0	1.4881643191356808E-4	0.10609784837482329	2.5009428141030193E-4
62-63	0.003809287278009805	1.8188675011658321E-4	0.0	1.550171165766334E-4	0.1083300948535268	2.6042875584874416E-4
64-65	0.0038320231217743783	1.860205398919601E-4	0.0	1.632846961273872E-4	0.11087030867049591	2.6042875584874416E-4
66-67	0.003846491385988197	2.08756383656533E-4	0.0	1.6948538079045254E-4	0.11321830126290998	2.6042875584874416E-4
68-69	0.0038754279144158355	2.2942533253341748E-4	0.0	1.6948538079045254E-4	0.11507023908227883	2.6042875584874416E-4
70-71	0.0039271002866080465	2.3975980697185968E-4	0.0	1.736191705658294E-4	0.11708132780799968	2.64562545624121E-4
72-73	0.003964304394586438	2.4389359674723658E-4	0.0	1.7981985522889476E-4	0.11870384029483512	2.686963353994979E-4
74-75	0.003999441607677142	2.4802738652261345E-4	0.0	1.942881194427139E-4	0.12035322241521049	2.810977047256286E-4
76-77	0.004022177451441715	2.5009428141030193E-4	0.0	1.9842190921809077E-4	0.1216946371973203	2.8523149450100546E-4
78-79	0.004040779505430911	2.5629496607336725E-4	0.0	2.0255569899346764E-4	0.12300711545100246	2.8523149450100546E-4
80-81	0.004075916718521614	2.6662944051180943E-4	0.0	2.0668948876884454E-4	0.12454281835255497	2.872983893886939E-4
82-83	0.004115187721387695	2.728301251748748E-4	0.0	2.08756383656533E-4	0.12623353837068413	2.8936528427638236E-4
84-85	0.004158592514029153	2.790308098379402E-4	0.0	2.1082327854422144E-4	0.12787465291150873	2.8936528427638236E-4
86-87	0.0042226662555474945	2.810977047256286E-4	0.0	2.1082327854422144E-4	0.1294392923414889	2.8936528427638236E-4
88-89	0.004253669678862821	2.8936528427638236E-4	0.0	2.1495706831959835E-4	0.13093779113506301	2.9143217916407084E-4
90-91	0.004282606207290459	2.9349907405175926E-4	0.0	2.1495706831959835E-4	0.13290754196303012	2.9349907405175926E-4
92-93	0.004334278579482671	2.9763286382713616E-4	0.0	2.1495706831959835E-4	0.1347181418846452	2.9349907405175926E-4
94-95	0.004385950951674881	2.9763286382713616E-4	0.0	2.1495706831959835E-4	0.13610502835428412	2.9349907405175926E-4
96-97	0.004462426062519354	3.038335484902015E-4	0.0	2.1702396320728677E-4	0.13737203492043715	2.9349907405175926E-4
98-99	0.004545101858026892	3.183018127040206E-4	0.0	2.27358437645729E-4	0.1384922919495643	2.9349907405175926E-4
100-101	0.00469391828994046	3.2656939225477437E-4	0.0	2.3975980697185968E-4	0.13977376677993114	2.9349907405175926E-4
102-103	0.004716654133705033	3.2656939225477437E-4	0.0	2.3975980697185968E-4	0.1408526859113045	2.955659689394477E-4
104-105	0.004747657557020359	3.2656939225477437E-4	0.0	2.4182670185954813E-4	0.1419295381477902	2.9763286382713616E-4
106-107	0.0047910623496618164	3.3070318203015127E-4	0.0	2.5216117629799035E-4	0.1430332600178158	2.9969975871482464E-4
108-109	0.0048386009320786506	3.3277007691783975E-4	0.0	2.728301251748748E-4	0.14401090129969246	3.059004433778899E-4
110-111	0.004884072619607797	3.3897076158090507E-4	0.0	2.7489702006256323E-4	0.14513529211859497	3.059004433778899E-4
112-113	0.004952280150901515	3.43104551356282E-4	0.0	2.8523149450100546E-4	0.1463113553096897	3.059004433778899E-4
114-115	0.004991551153767596	3.472383411316588E-4	0.0	2.8523149450100546E-4	0.14759489703494422	3.059004433778899E-4
116-117	0.00503908973618443	3.472383411316588E-4	0.0	2.8936528427638236E-4	0.14860560863502387	3.121011280409553E-4
118-119	0.005096962793039707	3.61706605345478E-4	0.0	2.9349907405175926E-4	0.14952330996515753	3.141680229286437E-4
120-121	0.005223043381188702	3.6790729000854333E-4	0.0	2.955659689394477E-4	0.15039760650264972	3.141680229286437E-4
122-123	0.00541113081596835	3.6790729000854333E-4	0.0	3.141680229286437E-4	0.15124296651171432	3.141680229286437E-4
124-125	0.005524810034791215	3.6790729000854333E-4	0.0	3.141680229286437E-4	0.15215240026229723	3.183018127040206E-4
126-127	0.005566147932544984	3.720410797839202E-4	0.0	3.141680229286437E-4	0.1530556333282171	3.183018127040206E-4
128-129	0.0056260878842879485	3.720410797839202E-4	0.0	3.1623491781633214E-4	0.15381211685711105	3.2450249736708594E-4
130-131	0.00571703125934624	3.720410797839202E-4	0.0	3.183018127040206E-4	0.15457686796555578	3.3070318203015127E-4
132-133	0.005793506370190713	3.720410797839202E-4	0.0	3.2243560247939747E-4	0.15532095012512362	3.3070318203015127E-4
134-135	0.005884449745249005	3.720410797839202E-4	0.0	3.2656939225477437E-4	0.1561105039722206	3.3070318203015127E-4
136-137	0.006041533756713326	3.782417644469855E-4	0.0	3.2656939225477437E-4	0.15693726192729598	3.3277007691783975E-4
138-139	0.006056002020927145	3.9271002866080463E-4	0.0	3.2656939225477437E-4	0.15771028061529146	3.3483697180552817E-4
>>END_MODULE
