##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24190877
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01341274233258	37.0	35.0	38.0	33.0	39.0
2	35.31389014957994	35.0	34.0	37.0	33.0	39.0
3	35.473415122568724	35.0	34.0	37.0	33.0	39.0
4	35.51321115807418	36.0	35.0	38.0	33.0	39.0
5	35.43183523276151	36.0	35.0	37.0	33.0	39.0
6	35.383308343885176	35.0	35.0	37.0	33.0	39.0
7	35.43156331207008	35.0	34.0	38.0	33.0	39.0
8	35.51991046045995	36.0	35.0	38.0	33.0	39.0
9	35.50194835846588	36.0	35.0	38.0	33.0	39.0
10-14	35.48179580260773	35.8	34.6	37.8	33.0	39.0
15-19	35.45572995968687	36.0	34.4	38.0	33.0	39.0
20-24	35.4452754234582	36.0	34.0	38.0	33.0	39.0
25-29	35.42721884783259	36.0	34.0	38.0	33.0	39.0
30-34	35.41235250793098	36.0	34.0	38.0	33.0	39.0
35-39	35.38130085155656	36.0	34.0	38.0	32.8	39.0
40-44	35.36894374685134	36.0	34.0	38.0	32.0	39.0
45-49	35.33790824532736	36.0	34.0	38.0	32.0	39.0
50-54	35.31833456058662	36.0	34.0	38.0	32.0	39.0
55-59	35.28981948856175	36.0	34.0	38.0	32.0	39.0
60-64	35.24782436783916	36.0	34.0	38.0	32.0	39.0
65-69	35.21954957470633	36.0	34.0	38.0	32.0	39.0
70-74	35.186169785977555	36.0	34.0	38.0	32.0	39.0
75-79	35.13463153957973	36.0	34.0	38.0	32.0	39.0
80-84	35.090436541551824	36.0	34.0	38.0	32.0	39.0
85-89	35.04214872756832	35.6	34.0	38.0	32.0	39.0
90-94	34.98818942531878	35.2	34.0	38.0	31.0	39.0
95-99	34.917312623419456	35.0	34.0	37.4	31.0	39.0
100-104	34.86866122447782	35.0	34.0	37.2	31.0	39.0
105-109	34.80792405206613	35.0	34.0	37.0	31.0	39.0
110-114	34.72938995011312	35.0	34.0	37.0	30.6	39.0
115-119	34.65477819815334	35.0	34.0	37.0	30.0	39.0
120-124	34.57960369510711	35.0	34.0	37.0	30.0	39.0
125-129	34.499744603633246	35.0	34.0	37.0	30.0	39.0
130-134	34.393505437222764	35.0	34.0	37.0	29.8	39.0
135-139	34.33753681066943	35.0	34.0	37.0	29.4	39.0
140-144	34.2144978306928	35.0	34.0	37.0	29.0	39.0
145-149	34.20495220796767	35.0	34.0	37.0	29.0	39.0
150	35.02239840127477	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	180.0
22	1703.0
23	9523.0
24	32743.0
25	79187.0
26	155501.0
27	266459.0
28	389889.0
29	514022.0
30	658169.0
31	854670.0
32	1136533.0
33	1579298.0
34	2345555.0
35	3926801.0
36	9957575.0
37	2283053.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.05954236384237	19.19324380013176	14.311924284514365	32.4352895515115
2	23.67759548361971	27.09440835898591	32.09056455456328	17.1374316028311
3	19.66068448035183	30.17669843056951	28.619429547758852	21.543187541319814
4	23.116627809731742	34.73010507225513	21.431885251617793	20.721381866395337
5	24.88265307619893	35.30454890081083	21.78958208088115	18.023215942109086
6	18.98554153286795	39.042482833507854	22.218012186990986	19.753963446633207
7	20.294716888519584	21.016811420272198	36.41687318735902	22.271598503849198
8	21.55364189566174	23.991899921610944	27.84223573209024	26.612222450637073
9	23.42972931489834	23.96846960116411	27.555334186519985	25.04646689741757
10-14	23.09135877959282	29.00538992447442	25.019611318762852	22.883639977169906
15-19	23.13720250820175	27.95274185388153	26.5725942883344	22.337461349582323
20-24	23.028773202393612	27.85146648465866	26.527083743181368	22.59267656976636
25-29	23.111253883023753	27.431244431526814	26.51101983611425	22.946481849335186
30-34	23.019428357227394	27.037513356791486	26.741018111910535	23.202040174070582
35-39	23.318652730118053	26.72909212840857	26.785867250699507	23.166387890773866
40-44	23.5140867360865	26.300153566156364	26.828810712402035	23.3569489853551
45-49	23.415131443272035	26.084635344842066	26.95106115376521	23.54917205812069
50-54	23.636984306108456	26.105866273471605	26.652143285255843	23.605006135164093
55-59	23.655783955248914	26.08498732807413	26.691597001629997	23.567631715046957
60-64	23.353302982773215	26.041768555972567	26.781382915551184	23.823545545703034
65-69	23.574358466135962	25.96811156167312	26.744159580640535	23.71337039155038
70-74	23.794192682168593	25.93487619520084	26.45547918165958	23.81545194097099
75-79	23.63049016231993	26.04733479499307	26.703300013057003	23.618875029629997
80-84	23.953743663900955	26.03237587028222	26.391435882392265	23.62244458342456
85-89	23.66082810565249	26.17236618020305	26.55975595328104	23.607049760863415
90-94	23.65298835228538	25.92145322182562	26.60230701855186	23.82325140733714
95-99	23.701205684376696	26.087954072895727	26.66735652302343	23.543483719704152
100-104	23.942834997534884	25.84346894978637	26.61258342338581	23.601112629292935
105-109	23.736148060745247	26.080936729856724	26.488628143411923	23.694287065986103
110-114	23.843420039205927	26.190049976206826	26.410222924945238	23.55630705964201
115-119	23.993422215063866	26.266634939137134	26.561505836175982	23.178437009623014
120-124	23.94136849697738	26.429573457866052	26.704919402478115	22.924138642678457
125-129	23.93906723322566	26.49665696408054	26.498406593138093	23.065869209555707
130-134	24.10571041106217	26.542301039190896	26.460289196442538	22.891699353304396
135-139	23.892916548793618	26.57574955349381	26.719009962996022	22.812323934716545
140-144	23.91867257337684	26.71577766819157	26.55039929409962	22.81515046433197
145-149	23.67899990974779	26.958569885630624	26.398332506133844	22.96409769848774
150	23.91706144532672	25.72119346697234	26.67394918383989	23.687795903861044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.0
10	2.5
11	7.0
12	10.0
13	9.5
14	19.5
15	33.0
16	49.5
17	104.5
18	200.0
19	333.5
20	489.5
21	763.0
22	1222.5
23	1900.0
24	3009.0
25	4792.0
26	7498.0
27	11541.5
28	17168.5
29	24210.0
30	34303.5
31	49314.0
32	70667.0
33	97002.0
34	130791.5
35	185111.0
36	262705.0
37	353018.0
38	463830.5
39	603767.5
40	771304.0
41	953445.0
42	1144149.5
43	1330654.0
44	1470642.0
45	1569841.0
46	1612387.5
47	1578782.5
48	1530237.5
49	1490564.0
50	1397683.5
51	1243892.0
52	1075573.0
53	918431.5
54	771842.5
55	655723.0
56	553572.5
57	449922.0
58	374549.5
59	312109.0
60	240877.5
61	166978.5
62	105069.5
63	63705.0
64	38555.5
65	19190.5
66	10822.5
67	5998.0
68	3176.0
69	1981.5
70	1294.0
71	1101.0
72	957.5
73	789.0
74	666.0
75	398.0
76	121.5
77	47.0
78	12.5
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.267579550753782E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.267580439341208E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	2.4803080284619316E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	7.4417109386453255E-6
130-134	0.050625956663258274
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	2.0
66-67	1.0
68-69	1.0
70-71	0.0
72-73	6.0
74-75	7.0
76-77	6.0
78-79	12.0
80-81	7.0
82-83	21.0
84-85	25.0
86-87	23.0
88-89	38.0
90-91	41.0
92-93	46.0
94-95	57.0
96-97	80.0
98-99	65.0
100-101	100.0
102-103	104.0
104-105	112.0
106-107	122.0
108-109	140.0
110-111	155.0
112-113	147.0
114-115	166.0
116-117	187.0
118-119	203.0
120-121	208.0
122-123	293.0
124-125	314.0
126-127	378.0
128-129	436.0
130-131	540.0
132-133	670.0
134-135	993.0
136-137	1667.0
138-139	4768.0
140-141	9157.0
142-143	10249.0
144-145	62419.0
146-147	799549.0
148-149	1091670.0
150-151	2.2205692E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.13675577631453
#Duplication Level	Percentage of total
1	24.94568192270178
2	8.271686424622708
3	5.116475748779332
4	3.50067465927257
5	2.623378528935257
6	2.088960488191591
7	1.784539576624845
8	1.5146787604327958
9	1.2829515918856509
>10	15.99202185315245
>50	5.7921509484251255
>100	13.41544014839946
>500	4.675905348870083
>1k	6.746073622963723
>5k	1.1732751756847137
>10k+	1.0761052010581658
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	27697	0.11449357540861374	No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA	24305	0.10047176049053533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.844424491100509E-4	4.133789775376891E-6	0.0	0.0	0.002116500364992968	0.0
2	0.0018850081375718624	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.011702758854091978	0.0
3	0.002810977047256286	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.017010544925675906	0.0
4	0.007101850834097499	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.025083836356986976	0.0
5	0.007436687805903027	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.030738860769702562	0.0
6	0.008916584545487953	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.04096585667398499	0.0
7	0.011942518661063838	4.133789775376891E-5	4.133789775376891E-6	0.0	0.05678587014435235	0.0
8	0.022272859309730688	4.133789775376891E-5	4.133789775376891E-6	0.0	0.07604519670783329	0.0
9	0.023054145577276922	4.133789775376891E-5	4.133789775376891E-6	4.133789775376891E-6	0.08957095685286648	4.133789775376891E-6
10-11	0.024077258546682703	4.133789775376891E-5	4.133789775376891E-6	4.133789775376891E-6	0.15095153433255024	4.133789775376891E-6
12-13	0.025185114206483707	4.133789775376891E-5	4.133789775376891E-6	4.133789775376891E-6	0.18278584939272768	4.133789775376891E-6
14-15	0.02949045625753874	4.960547730452269E-5	4.133789775376891E-6	4.133789775376891E-6	0.19699781864047344	4.133789775376891E-6
16-17	0.03437866266692191	5.3739267079899587E-5	4.133789775376891E-6	4.133789775376891E-6	0.20626370842198072	4.133789775376891E-6
18-19	0.043086490828753335	5.993995174296492E-5	4.133789775376891E-6	4.133789775376891E-6	0.21490539594740612	4.133789775376891E-6
20-21	0.04990724395812521	7.234132106909559E-5	4.133789775376891E-6	4.133789775376891E-6	0.22008710143084104	4.133789775376891E-6
22-23	0.05295798081235335	7.854200573216093E-5	1.2401369326130671E-5	4.133789775376891E-6	0.22535148270978353	4.133789775376891E-6
24-25	0.05970432572576844	7.854200573216093E-5	1.6535159101507563E-5	4.133789775376891E-6	0.2305931281449614	4.133789775376891E-6
26-27	0.06550196588573452	8.060890061984937E-5	1.6535159101507563E-5	4.133789775376891E-6	0.2350865576307961	4.133789775376891E-6
28-29	0.0797284033976941	8.267579550753782E-5	1.6535159101507563E-5	4.133789775376891E-6	0.23917900950841922	4.133789775376891E-6
30-31	0.10889642405275343	8.68095852829147E-5	2.0668948876884454E-5	4.133789775376891E-6	0.24416435997752378	4.133789775376891E-6
32-33	0.13431509738154596	9.301026994598004E-5	2.0668948876884454E-5	4.133789775376891E-6	0.24793644314755517	4.133789775376891E-6
34-35	0.15240249454370752	9.50771648336685E-5	2.0668948876884454E-5	4.133789775376891E-6	0.2522211162497333	4.133789775376891E-6
36-37	0.15558137888097234	9.50771648336685E-5	2.0668948876884454E-5	4.133789775376891E-6	0.2547096576945102	4.133789775376891E-6
38-39	0.15857837646812062	9.50771648336685E-5	2.0668948876884454E-5	4.133789775376891E-6	0.2565305920905637	4.133789775376891E-6
40-41	0.1602628958015867	9.50771648336685E-5	2.8936528427638237E-5	4.133789775376891E-6	0.25826471690133435	4.133789775376891E-6
42-43	0.160639070671146	1.0127784949673383E-4	2.8936528427638237E-5	4.133789775376891E-6	0.2599678382887896	4.133789775376891E-6
44-45	0.16161051126835957	1.1161232393517606E-4	2.8936528427638237E-5	4.133789775376891E-6	0.2614518688181499	4.133789775376891E-6
46-47	0.16311521074659674	1.1987990348592984E-4	2.8936528427638237E-5	4.133789775376891E-6	0.2629669027708256	4.133789775376891E-6
48-49	0.16457857232708017	1.2194679837361827E-4	2.8936528427638237E-5	6.200684663065336E-6	0.2643165851324861	8.267579550753781E-6
50-51	0.16573810035907338	1.2401369326130673E-4	2.8936528427638237E-5	8.267579550753781E-6	0.26560839443729134	8.267579550753781E-6
52-53	0.16697823729168645	1.2608058814899518E-4	2.8936528427638237E-5	8.267579550753781E-6	0.26695187611428883	1.0334474438442227E-5
54-55	0.16755696786023921	1.2814748303668363E-4	2.8936528427638237E-5	8.267579550753781E-6	0.26813413999004665	1.2401369326130673E-5
56-57	0.1683630568664377	1.3434816769974895E-4	2.8936528427638237E-5	8.267579550753781E-6	0.26929573491692754	1.2401369326130673E-5
58-59	0.16855527809099274	1.3848195747512585E-4	2.8936528427638237E-5	8.267579550753781E-6	0.2702609748294781	1.2401369326130673E-5
60-61	0.16892525227588895	1.4261574725050276E-4	2.8936528427638237E-5	1.0334474438442227E-5	0.2710050569890459	1.6535159101507563E-5
62-63	0.16929936025056058	1.4468264213819118E-4	3.100342331532668E-5	1.4468264213819119E-5	0.27187935352653814	1.6535159101507563E-5
64-65	0.16934069814831434	1.4468264213819118E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27273091422026574	1.6535159101507563E-5
66-67	0.16942957462848496	1.4674953702587963E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.2734398591667429	1.6535159101507563E-5
68-69	0.16948538079045253	1.4881643191356808E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.274208744064963	1.6535159101507563E-5
70-71	0.16961972895815228	1.4881643191356808E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27500656549161073	1.6535159101507563E-5
72-73	0.16968380269967062	1.508833268012565E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27550882094931906	1.6535159101507563E-5
74-75	0.169721006807649	1.5295022168894496E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27636451543282203	2.27358437645729E-5
76-77	0.16978301365427967	1.5708401146432186E-4	3.720410797839202E-5	1.6535159101507563E-5	0.27729255123739416	2.4802738652261346E-5
78-79	0.16983675292135958	1.6121780123969876E-4	3.720410797839202E-5	1.6535159101507563E-5	0.2784086744767459	2.4802738652261346E-5
80-81	0.1698863583986641	1.6948538079045254E-4	3.9271002866080465E-5	1.860205398919601E-5	0.2793243089119919	2.4802738652261346E-5
82-83	0.1699338969810809	1.6948538079045254E-4	4.133789775376891E-5	2.0668948876884454E-5	0.28008906002043665	2.4802738652261346E-5
84-85	0.16998763624816082	1.736191705658294E-4	4.5471687529145804E-5	2.27358437645729E-5	0.28092821934483814	2.4802738652261346E-5
86-87	0.17003310793569	1.736191705658294E-4	4.5471687529145804E-5	2.6869633539949793E-5	0.2818211179363196	2.6869633539949793E-5
88-89	0.17005584377945454	1.777529603412063E-4	4.753858241683425E-5	2.8936528427638237E-5	0.2826995482635871	2.8936528427638237E-5
90-91	0.17006824514878066	1.8808743477964854E-4	4.960547730452269E-5	2.8936528427638237E-5	0.28335682083787206	2.8936528427638237E-5
92-93	0.17007237893855603	1.90154329667337E-4	4.960547730452269E-5	2.8936528427638237E-5	0.2838900797188957	2.8936528427638237E-5
94-95	0.17013025199541132	1.9222122455502544E-4	4.960547730452269E-5	3.3070318203015126E-5	0.28448947923632534	2.8936528427638237E-5
96-97	0.17014265336473747	1.9635501433040232E-4	4.960547730452269E-5	3.720410797839202E-5	0.28509507943841805	2.8936528427638237E-5
98-99	0.17015712162895127	2.0048880410577922E-4	4.960547730452269E-5	3.720410797839202E-5	0.2857482182229276	2.8936528427638237E-5
100-101	0.17018605815737892	2.0255569899346767E-4	4.960547730452269E-5	4.133789775376891E-5	0.28626907573462507	2.8936528427638237E-5
102-103	0.17021292779091887	2.0255569899346767E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2867878663514349	2.8936528427638237E-5
104-105	0.1702439312142342	2.0255569899346767E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2873955334484153	2.8936528427638237E-5
106-107	0.17028113532221256	2.0255569899346767E-4	5.3739267079899587E-5	4.133789775376891E-5	0.28793912680387734	2.8936528427638237E-5
108-109	0.17031833943019098	2.08756383656533E-4	5.3739267079899587E-5	4.133789775376891E-5	0.28849098773889015	2.8936528427638237E-5
110-111	0.1703720786972709	2.1082327854422144E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2890015107761492	2.8936528427638237E-5
112-113	0.17039688143592313	2.1082327854422144E-4	5.3739267079899587E-5	4.133789775376891E-5	0.28963191371689423	2.8936528427638237E-5
114-115	0.17041548348991234	2.128901734319099E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2902085773905593	2.8936528427638237E-5
116-117	0.1704402862285646	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29081417759265193	2.8936528427638237E-5
118-119	0.1704588882825538	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29141357711008165	2.8936528427638237E-5
120-121	0.17046922275699222	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2919613042553191	3.100342331532668E-5
122-123	0.1704919586007568	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2924780279772412	3.3070318203015126E-5
124-125	0.1705126275496337	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2931063640230985	3.3070318203015126E-5
126-127	0.17053949718317363	2.1909085809497522E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2937119642251912	3.720410797839202E-5
128-129	0.17055189855249978	2.2322464787035212E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2943340995863854	3.720410797839202E-5
130-131	0.17055809923716284	2.314922274211059E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29509471690505473	3.9271002866080465E-5
132-133	0.17057463439626436	2.3975980697185968E-4	5.7873056855276475E-5	4.133789775376891E-5	0.29573958811001355	4.133789775376891E-5
134-135	0.17060563781957966	2.5216117629799035E-4	6.200684663065336E-5	4.5471687529145804E-5	0.2962501111472726	4.133789775376891E-5
136-137	0.17060977160935506	2.5216117629799035E-4	6.407374151834181E-5	4.5471687529145804E-5	0.2967937045027347	4.133789775376891E-5
138-139	0.17060977160935506	2.624956507364326E-4	6.614063640603025E-5	4.5471687529145804E-5	0.2973579668070736	4.133789775376891E-5
>>END_MODULE
