##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24083958
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	65-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.06707265475218	37.0	35.0	38.0	33.0	39.0
2	35.42978620872865	35.0	34.0	38.0	33.0	39.0
3	35.53213126347422	35.0	35.0	37.0	33.0	39.0
4	35.61996653540087	36.0	35.0	38.0	33.0	39.0
5	35.543485667928834	36.0	35.0	38.0	33.0	39.0
6	35.486062340749804	35.0	35.0	37.0	33.0	39.0
7	35.51682933511178	35.0	34.0	38.0	33.0	39.0
8	35.625253291008065	36.0	35.0	38.0	33.0	39.0
9	35.612883771014715	36.0	35.0	38.0	33.0	39.0
10-14	35.58135568912718	35.8	35.0	37.8	33.0	39.0
15-19	35.56208788439176	36.0	35.0	38.0	33.0	39.0
20-24	35.54760022418242	36.0	35.0	38.0	33.0	39.0
25-29	35.533609151784766	36.0	35.0	38.0	33.0	39.0
30-34	35.51582551339776	36.0	35.0	38.0	33.0	39.0
35-39	35.49000708272287	36.0	35.0	38.0	33.0	39.0
40-44	35.47433474182275	36.0	34.8	38.0	33.0	39.0
45-49	35.448200133881656	36.0	34.0	38.0	33.0	39.0
50-54	35.426970052015534	36.0	34.2	38.0	33.0	39.0
55-59	35.40419925993892	36.0	34.0	38.0	32.8	39.0
60-64	35.36256063060731	36.0	34.0	38.0	32.0	39.0
65-69	35.338010620861795	36.0	34.0	38.0	32.0	39.0
70-74	35.304275730210705	36.0	34.0	38.0	32.0	39.0
75-79	35.25531785079307	36.0	34.0	38.0	32.0	39.0
80-84	35.216370641258855	36.0	34.0	38.0	32.0	39.0
85-89	35.16843761920194	36.0	34.0	38.0	32.0	39.0
90-94	35.11684921717037	36.0	34.0	38.0	32.0	39.0
95-99	35.05062607504795	35.8	34.0	38.0	32.0	39.0
100-104	35.0059221290478	36.0	34.0	38.0	31.0	39.0
105-109	34.94716051684605	35.4	34.0	38.0	31.0	39.0
110-114	34.87173322036184	35.0	34.0	37.8	31.0	39.0
115-119	34.799239261660276	35.0	34.0	37.0	31.0	39.0
120-124	34.72475914073	35.0	34.0	37.0	30.4	39.0
125-129	34.64918509939383	35.0	34.0	37.0	30.0	39.0
130-134	34.563353787857835	35.0	34.0	37.0	30.0	39.0
135-139	34.4940289152814	35.0	34.0	37.0	30.0	39.0
140-144	34.374596722733884	35.0	34.0	37.0	29.6	39.0
145-149	34.36325304081325	35.0	34.0	37.0	29.4	39.0
150	35.13204241115809	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	151.0
22	1380.0
23	7803.0
24	27673.0
25	68694.0
26	137243.0
27	239919.0
28	357290.0
29	470032.0
30	602905.0
31	785173.0
32	1053372.0
33	1475332.0
34	2223310.0
35	3793328.0
36	1.0316724E7
37	2523613.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.31997589432767	18.98149797470997	15.639314767115936	32.05921136384642
2	24.516622226296857	25.726925781883526	32.360075532435324	17.396376459384292
3	19.846571730443973	29.51479985141977	29.914460073381626	20.724168344754627
4	23.440250144930495	34.30337322461698	21.468614087435295	20.787762543017223
5	25.509249767002583	35.238709517762814	21.4268103274387	17.825230387795894
6	19.025793019569292	39.274466431140596	21.667954245726552	20.03178630356356
7	20.457414018077927	20.81217298253053	36.72366062089961	22.00675237849194
8	21.478026992074977	23.615927249167267	27.897312393585803	27.008733365171956
9	23.64722609132602	23.729965813758685	27.304872396804548	25.317935698110748
10-14	23.088549647861036	29.06286084704184	25.025965416481792	22.822624088615335
15-19	23.23370851252938	27.844192387314408	26.55241468200534	22.36968441815087
20-24	23.109409176016666	27.78055998935059	26.52165063566379	22.588380198968956
25-29	23.201124997809746	27.333518851012776	26.516066835858126	22.94928931531935
30-34	23.052333839811546	26.99782319832978	26.760891212316512	23.188951749542163
35-39	23.3672380594585	26.70112777974451	26.784133239229202	23.147500921567794
40-44	23.568979816357427	26.336559796359055	26.81435667675554	23.28010371052798
45-49	23.43900948506886	26.12238569756682	26.932764954996184	23.505839862368138
50-54	23.681301055250138	26.131392522773872	26.63563107027508	23.55167535170091
55-59	23.713979238794554	26.135611098474758	26.682300309608582	23.468109353122106
60-64	23.391847801760825	26.081477969692525	26.7669882168039	23.759686011742755
65-69	23.60199882105751	26.021064777860754	26.733537406112607	23.643398994969132
70-74	23.806725067660203	26.0062769601217	26.444455895131156	23.742542077086938
75-79	23.64772907173892	26.112097513382697	26.709490018805564	23.530683396072813
80-84	23.979440571308164	26.09781602304358	26.4021604342566	23.52058297139166
85-89	23.661906563705053	26.22457918769196	26.59982070639806	23.513693542204933
90-94	23.683964433427633	25.964037791739848	26.63262524927482	23.7193725255577
95-99	23.69787634494526	26.157681608088602	26.69636267829335	23.448079368672794
100-104	23.944163993739103	25.901829039818868	26.65346618026914	23.500540786172884
105-109	23.75273116371813	26.155814749815846	26.497160958320205	23.594293128145814
110-114	23.869953885708934	26.260272704838144	26.41436182409973	23.455411585353193
115-119	23.980357391199014	26.3280231227003	26.595545066316216	23.09607441978447
120-124	23.958083242586095	26.487655030033565	26.713073155879258	22.841188571501085
125-129	23.928042164435066	26.56722942543539	26.519400837831213	22.98532757229833
130-134	24.121414589677368	26.601462236789736	26.477980735178136	22.799142438354764
135-139	23.862722728052386	26.63903882901205	26.750977754501697	22.747260688433858
140-144	23.92757918781831	26.788544033089394	26.54540442834554	22.738472350746754
145-149	23.678203501945372	27.027617015533323	26.405709085301705	22.888470397219603
150	23.965471053098796	25.794025746308325	26.653683235847147	23.586819964745732
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	3.5
10	4.5
11	4.0
12	6.5
13	9.0
14	13.0
15	24.0
16	46.0
17	85.5
18	175.0
19	308.0
20	467.0
21	698.0
22	1090.0
23	1699.0
24	2705.5
25	4402.0
26	6911.5
27	10712.5
28	16193.0
29	23121.0
30	32981.5
31	47601.5
32	68960.5
33	95324.0
34	129176.0
35	184409.0
36	262206.0
37	352253.5
38	462547.0
39	604065.0
40	774157.5
41	954728.0
42	1144877.0
43	1328488.5
44	1467310.0
45	1563986.0
46	1599315.0
47	1570791.5
48	1528525.0
49	1485369.0
50	1388392.5
51	1236792.5
52	1070873.0
53	909097.5
54	766960.5
55	654228.0
56	548638.5
57	447791.5
58	372666.0
59	310251.5
60	239168.5
61	164227.0
62	102776.0
63	62326.0
64	37950.0
65	19131.5
66	10998.5
67	6055.5
68	3158.5
69	1953.5
70	1207.0
71	990.5
72	850.5
73	696.5
74	575.5
75	339.5
76	109.0
77	35.5
78	9.5
79	3.0
80	1.0
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.304756182863988E-7
120-124	0.0
125-129	8.305160222777286E-7
130-134	0.0
135-139	8.306292820029688E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	1.0
68-69	2.0
70-71	1.0
72-73	8.0
74-75	8.0
76-77	11.0
78-79	9.0
80-81	19.0
82-83	16.0
84-85	20.0
86-87	25.0
88-89	32.0
90-91	45.0
92-93	47.0
94-95	46.0
96-97	55.0
98-99	76.0
100-101	76.0
102-103	82.0
104-105	102.0
106-107	88.0
108-109	106.0
110-111	134.0
112-113	135.0
114-115	141.0
116-117	162.0
118-119	190.0
120-121	226.0
122-123	237.0
124-125	281.0
126-127	312.0
128-129	376.0
130-131	496.0
132-133	605.0
134-135	846.0
136-137	1422.0
138-139	4245.0
140-141	8641.0
142-143	9560.0
144-145	60748.0
146-147	794245.0
148-149	1019502.0
150-151	2.2180578E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.52692260387857
#Duplication Level	Percentage of total
1	25.354402034163837
2	8.253901333254031
3	4.976632910059168
4	3.5459899535641264
5	2.7167981715495833
6	2.2160507390905706
7	1.773645055725929
8	1.4396039140094739
9	1.2992434726813
>10	15.94647740331906
>50	5.8792283225063535
>100	13.245047105025481
>500	4.727762964798988
>1k	6.714866923540806
>5k	1.134716491551144
>10k+	0.775633205160011
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32104	0.1333003487217508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.4427913385333093E-4	0.0	0.0	0.0	0.0021383528405090227	0.0
2	0.002092679284692325	4.152141437881598E-6	0.0	0.0	0.01042602715052069	0.0
3	0.0030227589667778027	4.152141437881598E-6	0.0	0.0	0.014723493538728144	0.0
4	0.007702222367270363	8.304282875763195E-6	0.0	0.0	0.021537157638291844	0.0
5	0.007984567985046312	1.2456424313644791E-5	0.0	0.0	0.026220773180222288	0.0
6	0.009143015446215277	1.2456424313644791E-5	0.0	0.0	0.034400491812849036	0.0
7	0.01198308018972629	2.0760707189407987E-5	0.0	0.0	0.04694826323812722	0.0
8	0.020715033633591288	2.0760707189407987E-5	0.0	0.0	0.06528827196925023	0.0
9	0.021437506243782687	2.0760707189407987E-5	0.0	0.0	0.07842149533726973	0.0
10-11	0.022135066005346794	2.4912848627289583E-5	0.0	0.0	0.13217304232136595	0.0
12-13	0.022992483212269346	2.4912848627289583E-5	0.0	0.0	0.158472706188908	0.0
14-15	0.026754323354990072	3.321713150305278E-5	0.0	0.0	0.16929318677602742	0.0
16-17	0.031188810410647617	3.321713150305278E-5	0.0	0.0	0.1773317325997662	0.0
18-19	0.039823188530722406	3.9445343659875174E-5	0.0	0.0	0.1852810073825905	0.0
20-21	0.047211924219432704	4.5673555816697566E-5	0.0	0.0	0.19041305419981217	0.0
22-23	0.05007067359941418	5.1901767973519965E-5	0.0	2.076070718940799E-6	0.1955181120976876	0.0
24-25	0.056942467679108225	5.6053909411401565E-5	0.0	4.152141437881598E-6	0.20050898610602128	0.0
26-27	0.06310632164364345	5.8129980130342364E-5	0.0	4.152141437881598E-6	0.20448258546207398	0.0
28-29	0.07810385651727178	5.8129980130342364E-5	0.0	4.152141437881598E-6	0.20789564572401265	0.0
30-31	0.10862624822713941	6.228212156822396E-5	2.076070718940799E-6	4.152141437881598E-6	0.21186716900934638	0.0
32-33	0.13209830377548407	6.228212156822396E-5	4.152141437881598E-6	4.152141437881598E-6	0.21496466652200608	0.0
34-35	0.14881690127511432	6.228212156822396E-5	4.152141437881598E-6	4.152141437881598E-6	0.21844623711766978	0.0
36-37	0.15155523855339725	6.228212156822396E-5	4.152141437881598E-6	4.152141437881598E-6	0.22058251388745986	0.0
38-39	0.15385344883926472	6.435819228716477E-5	4.152141437881598E-6	4.152141437881598E-6	0.2222433704626125	0.0
40-41	0.15543749079781655	6.851033372504636E-5	4.152141437881598E-6	4.152141437881598E-6	0.22396235701789546	0.0
42-43	0.15574890140565767	7.889068731975035E-5	4.152141437881598E-6	4.152141437881598E-6	0.22556300754219882	0.0
44-45	0.15666444859271056	8.927104091445434E-5	4.152141437881598E-6	4.152141437881598E-6	0.22683771496362848	0.0
46-47	0.158225653773354	9.549925307127675E-5	4.152141437881598E-6	4.152141437881598E-6	0.22820169342597257	2.076070718940799E-6
48-49	0.15953980653844355	9.965139450915833E-5	4.152141437881598E-6	4.152141437881598E-6	0.22948055298884013	4.152141437881598E-6
50-51	0.16075638397974285	9.965139450915833E-5	4.152141437881598E-6	4.152141437881598E-6	0.2308424553804653	4.152141437881598E-6
52-53	0.16200825462326418	1.0587960666598074E-4	4.152141437881598E-6	4.152141437881598E-6	0.23202996783169944	4.152141437881598E-6
54-55	0.162575021929535	1.0795567738492153E-4	4.152141437881598E-6	4.152141437881598E-6	0.23311782888842442	4.152141437881598E-6
56-57	0.16360475300612964	1.0795567738492153E-4	4.152141437881598E-6	4.152141437881598E-6	0.2342119181573062	4.152141437881598E-6
58-59	0.16377706687580174	1.1003174810386232E-4	4.152141437881598E-6	8.304282875763195E-6	0.23515653033442427	6.2282121568223966E-6
60-61	0.16409470569579968	1.1210781882280313E-4	8.304282875763195E-6	8.304282875763195E-6	0.2359620457733733	8.304282875763195E-6
62-63	0.16441026844507867	1.1210781882280313E-4	8.304282875763195E-6	8.304282875763195E-6	0.23696063578918383	1.2456424313644791E-5
64-65	0.1644310291522681	1.1418388954174394E-4	8.304282875763195E-6	8.304282875763195E-6	0.23783673763257684	1.2456424313644791E-5
66-67	0.164538984829653	1.2041210169856633E-4	8.304282875763195E-6	8.304282875763195E-6	0.23855505810133038	1.2456424313644791E-5
68-69	0.16460126695122124	1.2041210169856633E-4	8.304282875763195E-6	8.304282875763195E-6	0.2393875624596256	1.2456424313644791E-5
70-71	0.16475489618442285	1.2248817241750713E-4	8.304282875763195E-6	8.304282875763195E-6	0.24013287184772536	1.868463647046719E-5
72-73	0.16481510223527213	1.2456424313644793E-4	8.304282875763195E-6	8.304282875763195E-6	0.24065604166889842	2.0760707189407987E-5
74-75	0.1648483193667752	1.2664031385538872E-4	8.304282875763195E-6	8.304282875763195E-6	0.24137436213765195	2.0760707189407987E-5
76-77	0.16490229720546765	1.3286852601221113E-4	8.304282875763195E-6	8.304282875763195E-6	0.24217157329372524	2.0760707189407987E-5
78-79	0.16496457932703584	1.370206674500927E-4	1.2456424313644791E-5	8.304282875763195E-6	0.24315355474378422	2.0760707189407987E-5
80-81	0.16501440502429043	1.411728088879743E-4	1.2456424313644791E-5	8.304282875763195E-6	0.244037960870053	2.0760707189407987E-5
82-83	0.16504554608507455	1.494770917637375E-4	1.660856575152639E-5	8.304282875763195E-6	0.24477081383383908	2.0760707189407987E-5
84-85	0.16509121964089124	1.494770917637375E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24558255748494495	2.0760707189407987E-5
86-87	0.16512236070167535	1.536292332016191E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24637146435814244	2.0760707189407987E-5
88-89	0.16514727355030268	1.536292332016191E-4	2.4912848627289583E-5	8.304282875763195E-6	0.2470794044733013	2.0760707189407987E-5
90-91	0.16516595818677313	1.577813746395007E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24766485641604258	2.0760707189407987E-5
92-93	0.16517841461108676	1.598574453584415E-4	2.6988919346230383E-5	8.304282875763195E-6	0.24823577586375128	2.0760707189407987E-5
94-95	0.16521370781330877	1.619335160773823E-4	2.9064990065171182E-5	8.304282875763195E-6	0.24888973814021764	2.0760707189407987E-5
96-97	0.1652157838840277	1.619335160773823E-4	2.9064990065171182E-5	8.304282875763195E-6	0.2495125593558999	2.0760707189407987E-5
98-99	0.16525315315696865	1.619335160773823E-4	3.321713150305278E-5	8.304282875763195E-6	0.2501083916522359	2.4912848627289583E-5
100-101	0.16530505492494216	1.619335160773823E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2506523221805984	2.4912848627289583E-5
102-103	0.1653382720564452	1.702377989531455E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2512066330625556	2.4912848627289583E-5
104-105	0.16535695669291567	1.743899403910271E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2518003892881726	2.4912848627289583E-5
106-107	0.1653777174001051	1.8061815254784947E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2524066019381034	2.4912848627289583E-5
108-109	0.16541093453160813	1.8684636470467188E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25296506496149845	2.4912848627289583E-5
110-111	0.16545660808742485	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2535650493992723	2.6988919346230383E-5
112-113	0.16547321665317638	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2541816424027977	2.9064990065171182E-5
114-115	0.1654960534310847	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2547484097090686	2.9064990065171182E-5
116-117	0.16551473806755518	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25530687273246366	2.9064990065171182E-5
118-119	0.16553342270402566	1.9930278901831666E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2558715639680156	2.9064990065171182E-5
120-121	0.16553965091618245	2.0553100117513907E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25649853732513567	2.9064990065171182E-5
122-123	0.16554795519905824	2.1175921333196147E-4	3.321713150305278E-5	1.660856575152639E-5	0.25697395751977314	2.9064990065171182E-5
124-125	0.16556248769409082	2.1175921333196147E-4	3.321713150305278E-5	1.660856575152639E-5	0.2575075076945409	3.321713150305278E-5
126-127	0.16557494411840445	2.1175921333196147E-4	3.321713150305278E-5	1.868463647046719E-5	0.25812825283950425	3.5293202221993575E-5
128-129	0.16559570482559388	2.1175921333196147E-4	3.321713150305278E-5	2.0760707189407987E-5	0.258836192954663	3.7369272940934374E-5
130-131	0.16559985696703175	2.1591135476984306E-4	3.321713150305278E-5	2.0760707189407987E-5	0.25952129629191345	3.7369272940934374E-5
132-133	0.1656185416035022	2.1591135476984306E-4	3.321713150305278E-5	2.0760707189407987E-5	0.2601524217904715	3.9445343659875174E-5
134-135	0.16563099802781586	2.2006349620772467E-4	3.7369272940934374E-5	2.0760707189407987E-5	0.26072126516746125	4.1521414378815974E-5
136-137	0.16563515016925373	2.2213956692666547E-4	3.7369272940934374E-5	2.0760707189407987E-5	0.26126934783726163	4.1521414378815974E-5
138-139	0.16563722623997268	2.2836777908348787E-4	3.7369272940934374E-5	2.0760707189407987E-5	0.261746844102618	4.359748509775677E-5
>>END_MODULE
