##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23055046
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	73-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.28159002588848	36.0	34.0	38.0	31.0	39.0
2	34.75891802601478	35.0	34.0	37.0	31.0	39.0
3	34.900223838200105	35.0	34.0	38.0	31.0	39.0
4	34.89146562535594	36.0	34.0	38.0	31.0	39.0
5	34.808860021359315	35.0	34.0	37.0	31.0	39.0
6	34.776809814215945	35.0	34.0	37.0	31.0	39.0
7	34.74188652670656	35.0	34.0	37.0	32.0	39.0
8	34.877218505658156	35.0	34.0	37.0	32.0	39.0
9	34.849946384839136	35.0	34.0	37.0	32.0	39.0
10-14	34.86008632557055	35.0	34.0	37.0	31.2	39.0
15-19	34.86064275907322	35.0	34.0	37.0	31.0	39.0
20-24	34.85593439284398	35.0	34.0	37.0	31.0	39.0
25-29	34.84619000977053	35.0	34.0	37.0	31.0	39.0
30-34	34.82719559960974	35.0	34.0	37.0	31.0	39.0
35-39	34.81939806149161	35.0	34.0	37.0	31.0	39.0
40-44	34.760477953503106	35.0	34.0	37.0	31.0	39.0
45-49	34.6738526654859	35.0	34.0	37.0	31.0	39.0
50-54	34.65084556326629	35.0	34.0	37.0	31.0	39.0
55-59	34.63530898181683	35.0	34.0	37.0	31.0	39.0
60-64	34.62648034621141	35.0	34.0	37.0	31.0	39.0
65-69	34.601876725815245	35.0	34.0	37.0	31.0	39.0
70-74	34.59544040459422	35.0	34.0	37.0	31.0	39.0
75-79	34.57458654925809	35.0	34.0	37.0	30.8	39.0
80-84	34.561731351514815	35.0	34.0	37.0	30.2	39.0
85-89	34.546799637214995	35.0	34.0	37.0	30.0	39.0
90-94	34.532227175087996	35.0	34.0	37.0	30.0	39.0
95-99	34.51176536584353	35.0	34.0	37.0	30.0	39.0
100-104	34.475713165212085	35.0	34.0	37.0	30.0	39.0
105-109	34.45568843016865	35.0	34.0	37.0	30.0	39.0
110-114	34.41674918471422	35.0	34.0	37.0	30.0	39.0
115-119	34.3948153224816	35.0	34.0	37.0	30.0	39.0
120-124	34.371124160355265	35.0	34.0	37.0	30.0	39.0
125-129	34.34358006445454	35.0	34.0	37.0	30.0	39.0
130-134	34.31028602998275	35.0	34.0	37.0	30.0	39.0
135-139	34.2865287716739	35.0	34.0	37.0	30.0	39.0
140-144	34.251308279340805	35.0	34.0	37.0	30.0	39.0
145-149	34.277617004865554	35.0	34.0	37.0	30.0	39.0
150	35.04088203037246	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	5.0
22	167.0
23	2121.0
24	16394.0
25	71737.0
26	188790.0
27	340271.0
28	494550.0
29	641093.0
30	807422.0
31	1030446.0
32	1350866.0
33	1852031.0
34	2708456.0
35	4463865.0
36	8178166.0
37	908662.0
38	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.50697318062171	10.679232650414143	15.792291197337017	38.02150297162712
2	22.924541211930496	12.197179488124828	36.10750970449622	28.77076959544846
3	21.644406234421783	17.160192310286433	28.285862787531034	32.90953866776075
4	26.571421792616267	24.67914050335829	22.287002978829477	26.462434725195966
5	26.15242994325228	30.5512672639161	24.38287637436422	18.913426418467402
6	19.557540199160954	36.318839180411075	25.550200106085352	18.573420514342622
7	16.09019665624825	25.18736390425661	40.25124960957495	18.4711898299202
8	18.56346884640535	24.345282905275713	31.60506295468453	25.486185293634406
9	19.66699290097716	22.997653784835602	32.793566203675354	24.541787110511887
10-14	20.280631951537266	29.225736478152836	27.2220122027802	23.271619367529702
15-19	20.496183800437258	27.81726657617294	27.99817653476978	23.688373088620022
20-24	20.656588733130658	27.99404217848722	27.73295439260861	23.61641469577351
25-29	20.662516893206757	28.18038242796109	27.586259963108876	23.57084071572328
30-34	20.62190105557908	27.921834494593856	27.63138837290419	23.824876076922873
35-39	20.694503236428606	27.86791809706277	27.680318220330175	23.757260446178446
40-44	20.73257364524617	27.947782089578705	27.596782729388664	23.72286153578646
45-49	20.846515112800077	27.73075229300655	27.546197910361542	23.876534683831828
50-54	20.970101093688672	27.717492970626413	27.62206712700876	23.690338808676152
55-59	20.94443362391233	27.742620590839916	27.57218940264035	23.7407563826074
60-64	20.889023832719	27.674049636389032	27.53205701531044	23.90486951558153
65-69	21.00701492679867	27.580036982469437	27.632632255893768	23.780315834838124
70-74	21.150004651922348	27.506629911058283	27.60677311219244	23.73659232482693
75-79	21.173563251824184	27.422708608576208	27.588359864579832	23.815368275019775
80-84	21.209601911202096	27.414613577056386	27.550020349619263	23.825764162122255
85-89	21.159396844898083	27.434461120100078	27.6427133644708	23.763428670531038
90-94	21.144204453323518	27.345116814447927	27.58333554472514	23.927343187503418
95-99	21.255510097035728	27.243029450083693	27.685350132196522	23.816110320684057
100-104	21.321578667274796	27.31395578684937	27.532262709570528	23.832202836305306
105-109	21.26726016928827	27.16256815428016	27.64164023683601	23.92853143959556
110-114	21.332010666925765	27.150353684583102	27.70962035946221	23.808015289028926
115-119	21.357279366895455	27.1608981875863	27.692446401984594	23.78937604353365
120-124	21.33396386288701	27.06254075637729	27.68971529735881	23.913780083376896
125-129	21.380245609169275	26.99851360607616	27.743459521889275	23.877781262865298
130-134	21.447430664340907	27.1141423655283	27.650459175564972	23.787967794565827
135-139	21.424612150371754	26.98208853527583	27.75149434276716	23.84180497158526
140-144	21.46955466323987	26.890704398694194	27.896857200773123	23.742883737292814
145-149	21.11647478356793	27.06806911847349	27.958589864156785	23.856866233801796
150	21.219115410140304	25.652129309614786	28.506213339986864	24.622541940258046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.5
11	3.0
12	10.5
13	19.5
14	34.5
15	73.0
16	148.5
17	312.0
18	661.5
19	1238.0
20	2331.0
21	4021.5
22	6247.0
23	9689.5
24	15081.5
25	23026.0
26	33745.5
27	47235.5
28	63587.0
29	87776.5
30	118463.0
31	150783.0
32	183298.5
33	218518.0
34	255808.5
35	300508.0
36	368920.0
37	461412.0
38	574179.0
39	713337.0
40	871978.0
41	1031410.5
42	1192953.5
43	1358020.0
44	1495412.0
45	1576894.5
46	1596504.5
47	1559562.0
48	1478700.0
49	1357232.5
50	1199890.0
51	1030303.0
52	860128.0
53	693248.5
54	539636.5
55	408127.5
56	306491.0
57	230011.0
58	172894.0
59	129288.5
60	91482.5
61	64194.0
62	47204.5
63	32546.0
64	21924.0
65	17372.5
66	16300.0
67	12878.0
68	7440.5
69	4276.5
70	3249.5
71	2677.0
72	2091.0
73	1303.0
74	651.0
75	185.5
76	57.0
77	41.5
78	22.5
79	2.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.771189786392098E-5
3	5.638678838463389E-5
4	7.37365694260597E-5
5	9.542379572784196E-5
6	1.0843613150891132E-4
7	1.9518503671604037E-4
8	2.1687226301782264E-4
9	1.82172700934971E-4
10-14	1.6308794178940261E-4
15-19	1.7783525567461456E-4
20-24	1.8130521188289972E-4
25-29	2.975487448604527E-4
30-34	4.285395917232176E-4
35-39	5.499880590131983E-4
40-44	3.2617588357880523E-4
45-49	2.2988459879889203E-4
50-54	3.2791086168294786E-4
55-59	4.80588934847495E-4
60-64	8.067648184263002E-4
65-69	5.317707889197011E-4
70-74	2.6371667411738566E-4
75-79	4.285396995317319E-4
80-84	2.1687244362691437E-4
85-89	2.966819249660182E-4
90-94	4.042514868625597E-4
95-99	5.222320154701433E-4
100-104	3.5914461122595834E-4
105-109	4.259441377126151E-4
110-114	2.992906940676984E-4
115-119	3.955872759873188E-4
120-124	3.0970586695950323E-4
125-129	4.2422362348846907E-4
130-134	4.0687929652391476E-4
135-139	3.3054813130860543E-4
140-144	3.2978548660416996E-4
145-149	3.366562929402451E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
72-73	1.0
74-75	3.0
76-77	3.0
78-79	6.0
80-81	4.0
82-83	12.0
84-85	18.0
86-87	11.0
88-89	14.0
90-91	15.0
92-93	27.0
94-95	27.0
96-97	30.0
98-99	50.0
100-101	49.0
102-103	41.0
104-105	44.0
106-107	51.0
108-109	54.0
110-111	81.0
112-113	70.0
114-115	68.0
116-117	70.0
118-119	67.0
120-121	102.0
122-123	84.0
124-125	102.0
126-127	124.0
128-129	129.0
130-131	151.0
132-133	228.0
134-135	329.0
136-137	576.0
138-139	1916.0
140-141	4652.0
142-143	7114.0
144-145	64700.0
146-147	717004.0
148-149	1143415.0
150-151	2.1113604E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.454014106183216
#Duplication Level	Percentage of total
1	36.34907495566163
2	11.136216757228503
3	6.81322869738707
4	4.720837471683239
5	3.49600946634224
6	2.83998639293494
7	2.2960961850921375
8	1.9795488369685235
9	1.7321243516580334
>10	18.964339243640048
>50	4.586532696322956
>100	4.422645645370063
>500	0.36517362706164525
>1k	0.2981856726490478
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	5.638678838463389E-5	0.0	0.0	1.6916036515390168E-4	2.6024671562138717E-5
2	0.0	1.2144846728998068E-4	0.0	8.674890520712906E-6	0.0019084759145568393	3.903700734320808E-5
3	8.674890520712906E-6	1.3446080307105005E-4	0.0	8.674890520712906E-6	0.00304488657277023	6.072423364499034E-5
4	2.1687226301782266E-5	1.4313569359176295E-4	0.0	8.674890520712906E-6	0.004493593289729285	6.939912416570325E-5
5	2.1687226301782266E-5	1.5614802937283231E-4	0.0	8.674890520712906E-6	0.005872900882522638	7.807401468641616E-5
6	3.4699562082851625E-5	1.5614802937283231E-4	0.0	8.674890520712906E-6	0.007061360883860305	8.241145994677261E-5
7	3.4699562082851625E-5	1.8651014619532747E-4	0.0	8.674890520712906E-6	0.008202108987334053	8.241145994677261E-5
8	5.2049343124277434E-5	1.9518503671604037E-4	0.0	1.3012335781069358E-5	0.010045523222985546	8.241145994677261E-5
9	8.241145994677261E-5	1.9518503671604037E-4	0.0	1.3012335781069358E-5	0.013263907606170033	8.674890520712907E-5
10-11	1.236171899201589E-4	1.9952248197639683E-4	0.0	1.3012335781069358E-5	0.017777019399570923	1.1711102202962423E-4
12-13	1.474731388521194E-4	2.0169120460657505E-4	0.0	1.3012335781069358E-5	0.021433485754051414	1.4313569359176295E-4
14-15	1.6482291989354522E-4	2.1036609512728796E-4	0.0	1.5181058411247585E-5	0.02541959794831899	1.6916036515390168E-4
16-17	1.800039783047928E-4	2.1687226301782264E-4	0.0	1.7349781041425812E-5	0.029880660398595604	1.7349781041425813E-4
18-19	2.0819737249710974E-4	2.2337843090835732E-4	0.0	1.7349781041425812E-5	0.033656406497735894	1.7349781041425813E-4
20-21	2.450656572101396E-4	2.602467156213872E-4	0.0	1.7349781041425812E-5	0.03710467547971928	1.82172700934971E-4
22-23	2.8193394192316945E-4	2.7542777403263477E-4	0.0	1.7349781041425812E-5	0.040975845374587413	1.9084759145568392E-4
24-25	3.404894529379816E-4	2.841026645533477E-4	0.0	1.7349781041425812E-5	0.0452503976786687	1.9301631408586215E-4
26-27	4.0555113184332834E-4	2.862713871835259E-4	0.0	1.7349781041425812E-5	0.04946639447173516	2.1036609512728796E-4
28-29	4.207321902545759E-4	2.862713871835259E-4	0.0	2.1687226301782263E-5	0.05390360097307982	2.2771587616871378E-4
30-31	4.684440881184969E-4	2.862713871835259E-4	0.0	3.25308394526734E-5	0.058483943168016236	2.4940310247049605E-4
32-33	5.161559859824179E-4	2.862713871835259E-4	0.0	3.4699562082851625E-5	0.06240282496074828	2.9711500033441704E-4
34-35	5.616991612161607E-4	2.862713871835259E-4	0.0	4.1205729973386306E-5	0.06580338204486774	3.274771171569122E-4
36-37	6.007361685593688E-4	2.9060883244388236E-4	0.0	4.337445260356453E-5	0.06947069201249913	3.339832850474469E-4
38-39	6.202546722309728E-4	3.036211682249517E-4	0.0	4.337445260356453E-5	0.07369970114134666	3.3832073030780335E-4
40-41	6.614604022043591E-4	3.036211682249517E-4	0.0	4.337445260356453E-5	0.07711543928387737	3.4482689819833803E-4
42-43	6.723040153552503E-4	3.101273361154864E-4	0.0	5.638678838463389E-5	0.08045744085698203	3.513330660888727E-4
44-45	6.98328686917389E-4	3.2097094926637754E-4	0.0	5.638678838463389E-5	0.08448692750385317	3.5567051134922917E-4
46-47	7.330282490002405E-4	3.361520076776251E-4	0.0	5.638678838463389E-5	0.08837978462502309	3.600079566095856E-4
48-49	7.590529205623792E-4	3.5783923397940734E-4	0.0	5.638678838463389E-5	0.09208179415473733	3.7302029239065493E-4
50-51	7.742339789736268E-4	3.773577376510114E-4	0.0	6.072423364499034E-5	0.09628044116676236	3.795264602811896E-4
52-53	8.284520447280825E-4	3.8169518291136784E-4	0.0	6.072423364499034E-5	0.10045306350722527	3.860326281717243E-4
54-55	8.870075557428946E-4	3.8169518291136784E-4	0.0	6.072423364499034E-5	0.10391000737972936	3.9037007343208075E-4
56-57	9.238758404559245E-4	3.9037007343208075E-4	0.0	6.072423364499034E-5	0.10798720592446442	3.947075186924372E-4
58-59	9.607441251689543E-4	4.0555113184332834E-4	0.0	6.723040153552502E-5	0.11192994366612845	3.9904496395279365E-4
60-61	9.911062419914495E-4	4.1639474499421947E-4	0.0	6.939912416570325E-5	0.11536953775759112	4.1422602236404124E-4
62-63	0.0010258058040743011	4.250696355149324E-4	0.0	7.156784679588148E-5	0.11878310717749165	4.1639474499421947E-4
64-65	0.0010583366435269745	4.272383581451106E-4	0.0	7.37365694260597E-5	0.12353911590547251	4.185634676243977E-4
66-67	0.0010670115340476875	4.359132486658235E-4	0.0	7.37365694260597E-5	0.1278808986110893	4.250696355149324E-4
68-69	0.0011147234319116084	4.5326302970724933E-4	0.0	7.37365694260597E-5	0.13079566182604885	4.3157580340546706E-4
70-71	0.001205809782379094	4.6193792022796224E-4	0.0	7.807401468641616E-5	0.13345668449327752	4.424194165563582E-4
72-73	0.0012687027386542626	4.684440881184969E-4	0.0	8.891762783730729E-5	0.13632156708774296	4.684440881184969E-4
74-75	0.0013077397459974706	4.814564238995663E-4	0.0	9.542379572784196E-5	0.13965055632506654	4.814564238995663E-4
76-77	0.0013381018628199658	4.901313144202792E-4	0.0	9.759251835802019E-5	0.14204699483141348	4.8579386915992273E-4
78-79	0.0013749701475329957	4.988062049409921E-4	0.0	1.14942299399446E-4	0.14436752804570419	4.8796259179010096E-4
80-81	0.0014140071548762038	5.118185407220615E-4	0.0	1.1711102202962423E-4	0.14698951370558966	4.923000370504574E-4
82-83	0.0014530441622194119	5.204934312427744E-4	0.0	1.1711102202962423E-4	0.14945968878136268	4.944687596806356E-4
84-85	0.0015267807316454714	5.204934312427744E-4	0.0	1.1711102202962423E-4	0.15184311495192854	4.944687596806356E-4
86-87	0.001580998797399927	5.248308765031308E-4	0.0	1.1927974465980246E-4	0.15389038911481678	4.944687596806356E-4
88-89	0.0016048547463318875	5.248308765031308E-4	0.0	1.2578591255033714E-4	0.15616754787650391	4.966374823108139E-4
90-91	0.0016395543084147392	5.335057670238437E-4	0.0	1.2578591255033714E-4	0.1588892947773776	5.118185407220615E-4
92-93	0.001676422593127769	5.335057670238437E-4	0.0	1.301233578106936E-4	0.16167176591189625	5.161559859824179E-4
94-95	0.0017284719362520465	5.35674489654022E-4	0.0	1.301233578106936E-4	0.16465809697365166	5.248308765031308E-4
96-97	0.0018130521188289973	5.486868254350913E-4	0.0	1.3229208044087182E-4	0.1670458605894779	5.248308765031308E-4
98-99	0.0019041384692964828	5.55192993325626E-4	0.0	1.3662952570122827E-4	0.16908445986184542	5.248308765031308E-4
100-101	0.0019561878124207603	5.682053291066954E-4	0.0	1.387982483314065E-4	0.17126402610517455	5.248308765031308E-4
102-103	0.001973537593462186	5.682053291066954E-4	0.0	1.4313569359176295E-4	0.17302502888087928	5.248308765031308E-4
104-105	0.0019952248197639684	5.703740517368736E-4	0.0	1.4313569359176295E-4	0.17462771490458098	5.26999599133309E-4
106-107	0.002049442885518424	5.725427743670518E-4	0.0	1.4313569359176295E-4	0.17624558198669393	5.291683217634873E-4
108-109	0.002116673287053949	5.725427743670518E-4	0.0	1.4530441622194118E-4	0.17792634202508206	5.291683217634873E-4
110-111	0.002162216462287692	5.725427743670518E-4	0.0	1.474731388521194E-4	0.17956155888823644	5.291683217634873E-4
112-113	0.002220771973302504	5.877238327782994E-4	0.0	1.474731388521194E-4	0.1812987057150092	5.291683217634873E-4
114-115	0.0022814962069474943	5.942300006688341E-4	0.0	1.4964186148229763E-4	0.18320284418430569	5.313370443936655E-4
116-117	0.002327039382181237	6.02904891189547E-4	0.0	1.5614802937283231E-4	0.18496818440527074	5.335057670238437E-4
118-119	0.0023855948931960492	6.267608401215075E-4	0.0	1.6048547463318877E-4	0.18652532725373872	5.335057670238437E-4
120-121	0.002470175075773	6.397731759025768E-4	0.0	1.6699164252372345E-4	0.1879415031312451	5.335057670238437E-4
122-123	0.002624154382515654	6.462793437931115E-4	0.0	1.6916036515390168E-4	0.18941189707450595	5.335057670238437E-4
124-125	0.002689216061421001	6.484480664232898E-4	0.0	1.7349781041425813E-4	0.1910688011639621	5.335057670238437E-4
126-127	0.0027217469008736744	6.50616789053468E-4	0.0	1.7349781041425813E-4	0.1925326889393324	5.335057670238437E-4
128-129	0.0027434341271754567	6.50616789053468E-4	0.0	1.7349781041425813E-4	0.1938556097437411	5.35674489654022E-4
130-131	0.002784639857148843	6.50616789053468E-4	0.0	1.8000397830479278E-4	0.19521973627812322	5.421806575445566E-4
132-133	0.002869220039725794	6.571229569440027E-4	0.0	1.8651014619532747E-4	0.19650578879781894	5.465181028049131E-4
134-135	0.003018861901208091	6.592916795741809E-4	0.0	1.8651014619532747E-4	0.19748822014928968	5.465181028049131E-4
136-137	0.003188022266361993	6.657978474647156E-4	0.0	1.8651014619532747E-4	0.1987027048221895	5.508555480652695E-4
138-139	0.0032140469379241315	6.788101832457849E-4	0.0	1.886788688255057E-4	0.1997458604073052	5.508555480652695E-4
>>END_MODULE
