##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23055046
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	60-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00691640346326	37.0	35.0	38.0	33.0	39.0
2	35.355013778762356	35.0	34.0	37.0	33.0	39.0
3	35.48226279834792	35.0	34.0	37.0	33.0	39.0
4	35.57235370512815	36.0	35.0	38.0	33.0	39.0
5	35.498191155203074	36.0	35.0	37.0	33.0	39.0
6	35.4387432582004	36.0	35.0	37.0	33.0	39.0
7	35.405169957153845	35.0	34.0	37.0	33.0	39.0
8	35.55526104784176	36.0	34.0	38.0	33.0	39.0
9	35.52789350322701	36.0	34.0	38.0	33.0	39.0
10-14	35.45139981069654	36.0	34.4	37.6	33.0	39.0
15-19	35.455835637890296	36.0	34.0	38.0	33.0	39.0
20-24	35.433871665231116	36.0	34.0	38.0	33.0	39.0
25-29	35.414034480781346	36.0	34.0	38.0	33.0	39.0
30-34	35.385469115958394	36.0	34.0	38.0	33.0	39.0
35-39	35.35880866166998	36.0	34.0	37.8	32.4	39.0
40-44	35.33754967133876	36.0	34.0	37.6	32.0	39.0
45-49	35.301070628963394	36.0	34.0	37.4	32.0	39.0
50-54	35.275984398382896	36.0	34.0	37.2	32.0	39.0
55-59	35.24107998743529	36.0	34.0	37.0	32.0	39.0
60-64	35.1970417736377	36.0	34.0	37.0	32.0	39.0
65-69	35.15539841162801	35.8	34.0	37.0	32.0	39.0
70-74	35.12010161329078	35.8	34.0	37.0	32.0	39.0
75-79	35.06960312952907	35.0	34.0	37.0	32.0	39.0
80-84	35.02426611319219	35.0	34.0	37.0	32.0	39.0
85-89	34.97547543848998	35.0	34.0	37.0	31.6	39.0
90-94	34.911052130755714	35.0	34.0	37.0	31.0	39.0
95-99	34.84201986376159	35.0	34.0	37.0	31.0	39.0
100-104	34.78181053214611	35.0	34.0	37.0	31.0	39.0
105-109	34.7103337999968	35.0	34.0	37.0	31.0	39.0
110-114	34.63909533466496	35.0	34.0	37.0	30.2	39.0
115-119	34.56119343857556	35.0	34.0	37.0	30.0	39.0
120-124	34.47687054557123	35.0	34.0	37.0	30.0	39.0
125-129	34.406940455572155	35.0	34.0	37.0	30.0	39.0
130-134	34.30638951590893	35.0	34.0	37.0	29.8	39.0
135-139	34.20484096686629	35.0	34.0	37.0	29.0	39.0
140-144	34.10248931820138	35.0	34.0	37.0	29.0	39.0
145-149	34.068943226908786	35.0	34.0	37.0	29.0	39.0
150	34.871646247925675	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	17.0
21	141.0
22	1760.0
23	10101.0
24	35569.0
25	86469.0
26	161674.0
27	259762.0
28	366228.0
29	477152.0
30	610741.0
31	797593.0
32	1074947.0
33	1524172.0
34	2346089.0
35	4112619.0
36	9815521.0
37	1374486.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.63998692520501	19.13023292167797	16.83676970325715	31.393010449859872
2	24.139899785929728	25.539636745899358	33.25392193969164	17.066541528479277
3	19.7450796561013	29.598046761565637	30.56296355092779	20.093910031405272
4	23.320275453810652	34.09118481442999	22.458212508368558	20.1303272233908
5	25.13249052430824	35.31593193593853	22.47205329679469	17.079524242958538
6	18.754406302203865	39.18866611673644	22.751817541374674	19.305110039685022
7	20.203368928433278	20.74665563451923	38.21882636885652	20.83114906819097
8	21.345592689148948	23.35712639034103	28.474661402742868	26.822619517767155
9	23.41490651676917	23.240619276198302	28.731495806297314	24.612978400735212
10-14	22.955395603417646	29.353508134345013	25.686789886429413	22.004306375807932
15-19	22.841976273011372	28.427194509016235	27.100415078774905	21.630414139197487
20-24	22.93936540347276	28.513405946042408	26.98962671329848	21.557601937186348
25-29	23.07012443176214	28.434004425755642	26.904716650749688	21.591154491732524
30-34	23.06284032625951	28.223032200612675	27.078742996901788	21.635384476226026
35-39	23.16461047182469	28.170953985517965	27.040784043545173	21.623651499112167
40-44	23.316425393382428	27.943774217583428	27.15610803812753	21.58369235090661
45-49	23.331215214231193	27.76615756914994	27.237791674759613	21.664835541859254
50-54	23.404843347525745	27.725061142797113	27.187989128280204	21.68210638139694
55-59	23.560826553978682	27.607889396533842	27.09996935161179	21.731314697875685
60-64	23.4772656181997	27.583174723075366	27.167030667977176	21.772528990747762
65-69	23.578816450597508	27.503177287778406	27.1185924154288	21.799413846195286
70-74	23.661481007536302	27.442053136682816	27.040223051828054	21.856242803952828
75-79	23.580594871093403	27.505464918388988	27.107984368798384	21.805955841719225
80-84	23.711811775078274	27.54862396004828	26.954185520312922	21.785378744560518
85-89	23.73108100634687	27.483041942686242	27.018104570981805	21.76777247998508
90-94	23.685650098546205	27.415146300809557	27.056557346159966	21.842646254484272
95-99	23.747443081754298	27.535626299518356	27.065730855416632	21.651199763310718
100-104	23.92240597584279	27.48938382048444	26.88327468969601	21.704935513976768
105-109	23.690188167967552	27.62952714313252	26.99224116063144	21.688043528268487
110-114	23.729034647460516	27.616389359493247	26.91177610957385	21.74279988347239
115-119	23.792561846875433	27.625542422269255	26.925610226408565	21.656285504446746
120-124	23.761093703107832	27.545381129245506	27.090031284603057	21.603493883043605
125-129	23.753051798495804	27.634860872938926	27.04437982739567	21.567707501169593
130-134	23.795551481028248	27.531494343737045	27.128823349355454	21.544130825879254
135-139	23.699224415168434	27.530405839831918	27.230543309116644	21.539826435883
140-144	23.740941146040726	27.65130124987251	27.19189372553966	21.415863878547107
145-149	23.455517553630187	27.77767474272531	27.129213749847665	21.637593953796838
150	23.684482633070182	26.564629672357064	27.481454322599102	22.26943337197365
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	10.0
3	25.0
4	35.0
5	46.0
6	49.0
7	37.0
8	27.5
9	20.5
10	13.0
11	15.0
12	19.0
13	16.0
14	23.0
15	47.0
16	80.5
17	134.0
18	208.5
19	385.0
20	650.0
21	982.5
22	1466.0
23	2520.0
24	4351.5
25	7053.5
26	10877.0
27	17447.0
28	29013.5
29	43618.0
30	58231.0
31	76419.0
32	102637.0
33	136195.0
34	181082.5
35	246787.0
36	344047.0
37	462456.5
38	599790.0
39	777882.0
40	986459.0
41	1178351.0
42	1349540.5
43	1504668.0
44	1613241.5
45	1662310.5
46	1658482.0
47	1602619.0
48	1502586.5
49	1363627.5
50	1185173.5
51	993838.0
52	811179.0
53	646556.5
54	504558.0
55	381677.5
56	282002.5
57	209555.5
58	153771.5
59	109902.0
60	76237.5
61	50985.0
62	34715.0
63	24623.0
64	17308.5
65	12371.5
66	9152.5
67	6762.0
68	5602.0
69	4168.5
70	2508.5
71	1805.5
72	1362.0
73	1070.5
74	908.0
75	553.0
76	180.5
77	68.5
78	22.5
79	2.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.337445260356453E-6
4	4.337445260356453E-6
5	4.337445260356453E-6
6	0.0
7	0.0
8	4.337445260356453E-6
9	8.674890520712906E-6
10-14	0.09597985621021966
15-19	2.6024671562138715E-6
20-24	8.674890520712907E-7
25-29	0.0
30-34	8.674890520712907E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	6.940220127646437E-6
100-104	0.0
105-109	0.0
110-114	8.675889628976014E-7
115-119	0.0
120-124	0.0
125-129	8.67684567678984E-7
130-134	8.677412554546431E-7
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	3.0
66-67	2.0
68-69	8.0
70-71	12.0
72-73	16.0
74-75	24.0
76-77	28.0
78-79	30.0
80-81	48.0
82-83	76.0
84-85	76.0
86-87	92.0
88-89	133.0
90-91	129.0
92-93	133.0
94-95	128.0
96-97	153.0
98-99	192.0
100-101	235.0
102-103	192.0
104-105	204.0
106-107	238.0
108-109	247.0
110-111	247.0
112-113	280.0
114-115	272.0
116-117	297.0
118-119	303.0
120-121	352.0
122-123	368.0
124-125	428.0
126-127	476.0
128-129	533.0
130-131	683.0
132-133	914.0
134-135	1228.0
136-137	1749.0
138-139	3455.0
140-141	7130.0
142-143	10848.0
144-145	66628.0
146-147	802732.0
148-149	964284.0
150-151	2.1189439E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.93670647072812
#Duplication Level	Percentage of total
1	24.872074728460117
2	11.229333789216597
3	7.468657064576602
4	5.392029472128241
5	4.0940551752111425
6	3.2478001550633206
7	2.7241279508729326
8	2.3172717554452604
9	1.9936266746751359
>10	22.426669755000063
>50	5.742073701899029
>100	6.493642382695667
>500	0.9959339561929164
>1k	0.9016858671351399
>5k	0.05510089530056925
>10k+	0.04591667612718488
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.337445260356453E-6	4.337445260356453E-6	4.337445260356453E-6	0.0	0.0015354556221661843	0.0
2	1.3012335781069358E-5	3.4699562082851625E-5	4.337445260356453E-6	0.0	0.005950974897209053	0.0
3	4.771189786392098E-5	3.903700734320808E-5	4.337445260356453E-6	0.0	0.007772701906558764	0.0
4	9.542379572784196E-5	3.903700734320808E-5	8.674890520712906E-6	8.674890520712906E-6	0.009186709061434968	0.0
5	9.976124098819841E-5	3.903700734320808E-5	8.674890520712906E-6	8.674890520712906E-6	0.01077421402672543	0.0
6	1.0409868624855487E-4	3.903700734320808E-5	8.674890520712906E-6	8.674890520712906E-6	0.012491842349826584	0.0
7	1.0843613150891132E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.014647552644223742	0.0
8	1.5614802937283231E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.02032526849003034	0.0
9	1.6482291989354522E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.027069995869884622	0.0
10-11	1.9084759145568392E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.03423979288525384	0.0
12-13	2.212097082781791E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.04068523654214353	0.0
14-15	2.4940310247049605E-4	6.50616789053468E-5	8.674890520712906E-6	8.674890520712906E-6	0.049986887902977936	0.0
16-17	2.689216061421001E-4	6.939912416570325E-5	8.674890520712906E-6	8.674890520712906E-6	0.05608099849377876	0.0
18-19	3.1446478137584286E-4	6.939912416570325E-5	8.674890520712906E-6	1.3012335781069358E-5	0.06480576963498576	0.0
20-21	3.621766792397638E-4	7.37365694260597E-5	8.674890520712906E-6	1.3012335781069358E-5	0.07085867449581319	0.0
22-23	3.92538796062259E-4	9.542379572784196E-5	8.674890520712906E-6	1.5181058411247585E-5	0.07724773136431826	0.0
24-25	4.489255844468929E-4	9.759251835802019E-5	8.674890520712906E-6	2.1687226301782266E-5	0.083049064400045	0.0
26-27	5.031436502013485E-4	1.0409868624855487E-4	8.674890520712906E-6	2.1687226301782266E-5	0.08750362068243109	0.0
28-29	5.855551101481212E-4	1.1277357676926778E-4	1.0843613150891131E-5	2.1687226301782266E-5	0.09201022630794144	0.0
30-31	7.330282490002405E-4	1.14942299399446E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.0974211892702361	0.0
32-33	8.52307993660043E-4	1.2795463518051536E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.10304251832765807	0.0
34-35	9.824313514707366E-4	1.301233578106936E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.10628042121451417	0.0
36-37	0.0010366494172251923	1.4313569359176295E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.11000411797053018	0.0
38-39	0.0010886987603494697	1.4964186148229763E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.11364106582133907	0.0
40-41	0.001119060877171965	1.62654197263367E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.11649510480265363	0.0
42-43	0.0011364106582133907	1.8434142356514924E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.11941420546287351	0.0
44-45	0.0011602666071453512	2.1036609512728796E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.12198414177963471	0.0
46-47	0.0012101472276394504	2.4072821194978314E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.12460395871689	2.1687226301782265E-6
48-49	0.0012426780670921238	2.450656572101396E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.12723678798992638	6.506167890534679E-6
50-51	0.0013077397459974706	2.5590927036103073E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1296137079926017	8.674890520712906E-6
52-53	0.0013619578117519263	2.5590927036103073E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.13204701478366168	8.674890520712906E-6
54-55	0.0013923199285744215	2.602467156213872E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1341246510633724	8.674890520712906E-6
56-57	0.0014400318264383425	2.6675288351192186E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.13649289617552704	8.674890520712906E-6
58-59	0.0014834062790419068	2.7325905140245654E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.13862908796625262	8.674890520712906E-6
60-61	0.0015549741258377883	2.949462777042388E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1401450250847472	8.674890520712906E-6
62-63	0.0015983485784413528	3.1229605874566463E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.14212506884609988	8.674890520712906E-6
64-65	0.0016395543084147392	3.2964583978709045E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.14397282052701177	1.0843613150891131E-5
66-67	0.001704615987320086	3.600079566095856E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.14549526381339686	1.3012335781069358E-5
68-69	0.0017349781041425811	3.643454018699421E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.14702638199030268	1.5181058411247585E-5
70-71	0.0017675089435952545	3.795264602811896E-4	1.7349781041425812E-5	2.6024671562138717E-5	0.14848376359778245	2.1687226301782266E-5
72-73	0.0018217270093497102	3.947075186924372E-4	1.7349781041425812E-5	2.6024671562138717E-5	0.14949655706607567	2.6024671562138717E-5
74-75	0.0018455829582816707	3.9904496395279365E-4	1.7349781041425812E-5	2.6024671562138717E-5	0.15058308710379498	2.8193394192316944E-5
76-77	0.00185859529406274	4.337445260356453E-4	2.6024671562138717E-5	2.6024671562138717E-5	0.15195155108343744	3.036211682249517E-5
78-79	0.0018889574108852353	4.662753654883187E-4	3.25308394526734E-5	2.6024671562138717E-5	0.1535694181655504	3.036211682249517E-5
80-81	0.0018998010240361264	4.923000370504574E-4	3.4699562082851625E-5	2.6024671562138717E-5	0.1548836640794384	3.036211682249517E-5
82-83	0.0019019697466663046	5.053123728315268E-4	3.4699562082851625E-5	2.6024671562138717E-5	0.1565752677309774	3.036211682249517E-5
84-85	0.0019193195277077304	5.096498180918832E-4	3.4699562082851625E-5	2.6024671562138717E-5	0.15825602776936554	3.036211682249517E-5
86-87	0.0019366693087491563	5.118185407220615E-4	3.4699562082851625E-5	2.8193394192316944E-5	0.16007992350134542	3.036211682249517E-5
88-89	0.0019453441992698692	5.139872633522397E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16197972452538156	3.036211682249517E-5
90-91	0.0019475129219000474	5.291683217634873E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16310962901570442	3.036211682249517E-5
92-93	0.0019475129219000474	5.291683217634873E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16413977226503906	3.4699562082851625E-5
94-95	0.0019561878124207603	5.291683217634873E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16525232697432052	3.686828471302985E-5
96-97	0.001973537593462186	5.400119349143784E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16630415744995694	3.903700734320808E-5
98-99	0.0019843812066130772	5.465181028049131E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16727140774301644	3.903700734320808E-5
100-101	0.001990887374503612	5.508555480652695E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16812805318193683	3.903700734320808E-5
102-103	0.001990887374503612	5.530242706954478E-4	3.686828471302985E-5	3.036211682249517E-5	0.16899554223400814	4.771189786392098E-5
104-105	0.0019952248197639684	5.595304385859825E-4	3.903700734320808E-5	3.036211682249517E-5	0.16986086256344923	5.2049343124277434E-5
106-107	0.002021249491326107	5.725427743670518E-4	3.903700734320808E-5	3.036211682249517E-5	0.17067630227239625	5.2049343124277434E-5
108-109	0.0020320931044769982	5.855551101481212E-4	3.903700734320808E-5	3.036211682249517E-5	0.1714852358134527	5.638678838463389E-5
110-111	0.0020429367176278894	6.072423364499034E-4	4.337445260356453E-5	3.036211682249517E-5	0.1723462186976335	6.072423364499034E-5
112-113	0.002047274162888246	6.332670080120422E-4	4.337445260356453E-5	3.036211682249517E-5	0.17306406588822248	7.156784679588148E-5
114-115	0.002047274162888246	6.441106211629333E-4	4.337445260356453E-5	3.036211682249517E-5	0.17392938621766357	7.590529205623793E-5
116-117	0.0020537803307787805	6.50616789053468E-4	4.337445260356453E-5	3.036211682249517E-5	0.17487928672968164	8.024273731659438E-5
118-119	0.0020559490534089587	6.527855116836462E-4	4.337445260356453E-5	3.036211682249517E-5	0.1756795453802174	8.891762783730729E-5
120-121	0.0020559490534089587	6.549542343138244E-4	4.337445260356453E-5	3.4699562082851625E-5	0.1765578780454396	9.108635046748552E-5
122-123	0.002060286498669315	6.549542343138244E-4	4.771189786392098E-5	3.4699562082851625E-5	0.17728656884917948	9.325507309766373E-5
124-125	0.00206679266655985	6.592916795741809E-4	5.2049343124277434E-5	3.4699562082851625E-5	0.17815188917862057	9.759251835802019E-5
126-127	0.0020841424476012757	6.636291248345373E-4	5.2049343124277434E-5	3.4699562082851625E-5	0.17915600775639312	1.0192996361837664E-4
128-129	0.0020993235060125233	6.679665700948938E-4	5.2049343124277434E-5	3.4699562082851625E-5	0.18013843910786387	1.0409868624855487E-4
130-131	0.0021036609512728797	6.939912416570325E-4	5.421806575445566E-5	3.4699562082851625E-5	0.18103195283149728	1.0409868624855487E-4
132-133	0.0021123358417935926	7.004974095475672E-4	5.638678838463389E-5	3.4699562082851625E-5	0.1820360714092698	1.0409868624855487E-4
134-135	0.0021210107323143055	7.070035774381019E-4	5.638678838463389E-5	3.4699562082851625E-5	0.18285801728610734	1.14942299399446E-4
136-137	0.0021210107323143055	7.070035774381019E-4	5.8555511014812115E-5	3.4699562082851625E-5	0.18372984378343898	1.2144846728998068E-4
138-139	0.0021210107323143055	7.135097453286366E-4	6.072423364499034E-5	3.4699562082851625E-5	0.18442600374772622	1.2144846728998068E-4
>>END_MODULE
