##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22208573
Total Bases	3.3 Gbp
Sequences flagged as poor quality	0
Sequence length	58-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.21567085827622	37.0	35.0	38.0	33.0	39.0
2	35.544918171914965	35.0	34.0	38.0	33.0	39.0
3	35.64621990796077	36.0	35.0	38.0	33.0	39.0
4	35.76538983391684	36.0	35.0	38.0	33.0	39.0
5	35.69358585083337	36.0	35.0	38.0	33.0	39.0
6	35.61481775528756	36.0	35.0	37.0	33.0	39.0
7	35.56882276947736	35.0	34.0	38.0	33.0	39.0
8	35.74717565149278	36.0	35.0	38.0	33.0	39.0
9	35.72764193359024	36.0	35.0	38.0	33.0	39.0
10-14	35.65015556830239	36.0	35.0	37.8	33.0	39.0
15-19	35.65950319275354	36.0	35.0	38.0	33.0	39.0
20-24	35.640471785377656	36.0	35.0	38.0	33.0	39.0
25-29	35.62757585550409	36.0	35.0	38.0	33.0	39.0
30-34	35.60151770219545	36.0	35.0	38.0	33.0	39.0
35-39	35.58125089802033	36.0	35.0	38.0	33.0	39.0
40-44	35.562949235864906	36.0	35.0	38.0	33.0	39.0
45-49	35.53377424114553	36.0	35.0	38.0	33.0	39.0
50-54	35.51247630363283	36.0	35.0	38.0	33.0	39.0
55-59	35.48528289031761	36.0	35.0	38.0	33.0	39.0
60-64	35.44758779120527	36.0	34.0	38.0	33.0	39.0
65-69	35.41349478486077	36.0	34.0	38.0	33.0	39.0
70-74	35.38393757749712	36.0	34.0	38.0	32.2	39.0
75-79	35.341453044872395	36.0	34.0	38.0	32.0	39.0
80-84	35.304443527043176	36.0	34.0	38.0	32.0	39.0
85-89	35.26286406010713	36.0	34.0	38.0	32.0	39.0
90-94	35.20792111179101	36.0	34.0	38.0	32.0	39.0
95-99	35.148598341663686	36.0	34.0	38.0	32.0	39.0
100-104	35.09949067805925	36.0	34.0	38.0	32.0	39.0
105-109	35.03694382072456	36.0	34.0	38.0	32.0	39.0
110-114	34.976063718525765	35.8	34.0	37.6	31.2	39.0
115-119	34.90846516391658	35.0	34.0	37.0	31.0	39.0
120-124	34.83381234506298	35.0	34.0	37.0	31.0	39.0
125-129	34.77397990055938	35.0	34.0	37.0	31.0	39.0
130-134	34.68702897633961	35.0	34.0	37.0	30.2	39.0
135-139	34.59722536112652	35.0	34.0	37.0	30.0	39.0
140-144	34.50446990309907	35.0	34.0	37.0	30.0	39.0
145-149	34.47213192456565	35.0	34.0	37.0	30.0	39.0
150	35.16616293158933	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	127.0
22	1303.0
23	7028.0
24	25034.0
25	62924.0
26	124024.0
27	208102.0
28	299878.0
29	389398.0
30	494697.0
31	643165.0
32	867758.0
33	1239293.0
34	1943761.0
35	3530530.0
36	1.0516933E7
37	1854603.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.22558365186273	18.87454452836749	17.00789150207895	30.89198031769083
2	24.76441867741795	24.92582931825471	33.09751148801862	17.212240516308725
3	20.181764942754313	29.116985589303734	30.717565689610044	19.98368377833191
4	23.90982527333026	33.56365129808205	22.514017447226347	20.012505981361343
5	25.68481549895169	35.11645705466984	22.315504017299986	16.88322342907849
6	19.05654631659585	39.24520499358513	22.60486074454221	19.093387945276806
7	20.467569909492607	20.9037123143262	38.099450968752066	20.52926680742913
8	21.50759272174693	23.423258524828093	28.520727425461097	26.548421327963872
9	23.67693879642509	23.206773492685617	28.754577286644096	24.361710424245196
10-14	23.133209551337412	29.461755648700645	25.659002830716737	21.74603196924521
15-19	23.078567002031154	28.38548609133959	27.094711578272047	21.44123532835721
20-24	23.163166072517726	28.43455928874457	27.00442326487544	21.39785137386226
25-29	23.28628768719179	28.365252463541896	26.90277758953716	21.44568225972916
30-34	23.22454237131348	28.173236778186844	27.121959858672234	21.480260991827443
35-39	23.334958981831026	28.104774674176497	27.08134736977473	21.478918974217752
40-44	23.45390313911659	27.89648303832939	27.200722892011115	21.448890930542902
45-49	23.479294234708373	27.70697694084172	27.273983789953547	21.539745034496363
50-54	23.52149187651979	27.68328388651146	27.245679895699375	21.54954434126937
55-59	23.68964024559021	27.563731605481646	27.15840704540906	21.588221103519086
60-64	23.580291642166742	27.5426418910918	27.225727320295313	21.65133914644615
65-69	23.691537309169725	27.453143785963203	27.20484053862847	21.650478366238605
70-74	23.775753303039608	27.386689068840532	27.117085804621542	21.72047182349832
75-79	23.694450999735555	27.446592463623087	27.198354433565886	21.660602103075473
80-84	23.817798489476036	27.491408643805652	27.02865725802759	21.662135608690722
85-89	23.82870908758911	27.460069701668687	27.085100019232495	21.626121191509707
90-94	23.785582650271778	27.37634072870836	27.13824882489307	21.69982779612679
95-99	23.838980388234535	27.491739599253766	27.14644944903299	21.52283056347871
100-104	24.026060448853386	27.43960113913416	26.95676851241159	21.577569899600864
105-109	23.80097781359849	27.57961121167299	27.071939966742754	21.547471007985763
110-114	23.842969684042494	27.572610224475223	26.983474590975305	21.600945500506985
115-119	23.90611889886601	27.578513073232436	27.006926258149598	21.508441769751954
120-124	23.883214671221843	27.504308623736033	27.15303469167612	21.459442013366
125-129	23.85646065537114	27.600032696273907	27.11302124604779	21.43048540230716
130-134	23.921413220878957	27.50741204596267	27.161581273343742	21.409593459814634
135-139	23.811346616539154	27.515662446006807	27.25696266989599	21.416028267558048
140-144	23.848036860830394	27.629092130264628	27.229288180101953	21.293582828803032
145-149	23.5980740761388	27.770166480950003	27.13550911760577	21.49625032530542
150	23.889415163137507	26.51467527009152	27.461832742316382	22.134076824454592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	8.5
3	22.5
4	33.0
5	37.5
6	38.0
7	39.5
8	31.5
9	23.0
10	19.0
11	17.0
12	17.0
13	15.5
14	26.0
15	44.0
16	64.5
17	119.5
18	201.0
19	345.5
20	603.0
21	996.5
22	1478.5
23	2405.5
24	3956.0
25	6403.0
26	10096.5
27	16068.5
28	26273.5
29	39372.5
30	53566.0
31	71034.0
32	95121.0
33	127654.5
34	171013.5
35	235442.5
36	329782.0
37	444431.0
38	578965.0
39	750992.0
40	951443.5
41	1135669.5
42	1299115.0
43	1453997.0
44	1565643.0
45	1612749.0
46	1604559.0
47	1548023.0
48	1449141.5
49	1310335.0
50	1136989.0
51	953454.5
52	778293.0
53	619175.0
54	482931.5
55	365761.0
56	270783.5
57	201592.0
58	147845.5
59	106295.5
60	74482.0
61	50062.0
62	34435.5
63	24532.0
64	17023.0
65	12133.0
66	9102.0
67	6841.5
68	5867.0
69	4484.5
70	2721.0
71	2001.0
72	1503.5
73	1124.0
74	971.0
75	580.5
76	181.5
77	66.5
78	20.0
79	3.5
80	2.5
81	2.0
82	1.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.5027656662136735E-6
8	9.005531332427347E-6
9	4.5027656662136735E-6
10-14	0.09827105955884693
15-19	0.0
20-24	9.005531332427347E-7
25-29	0.0
30-34	9.005531332427347E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.8011062664854694E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	3.6023540411023013E-6
100-104	0.0
105-109	9.006213350565702E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	9.008718782176891E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	0.0
62-63	1.0
64-65	1.0
66-67	1.0
68-69	2.0
70-71	9.0
72-73	8.0
74-75	14.0
76-77	24.0
78-79	38.0
80-81	39.0
82-83	72.0
84-85	69.0
86-87	86.0
88-89	113.0
90-91	126.0
92-93	99.0
94-95	101.0
96-97	128.0
98-99	143.0
100-101	177.0
102-103	162.0
104-105	168.0
106-107	192.0
108-109	209.0
110-111	187.0
112-113	197.0
114-115	184.0
116-117	231.0
118-119	253.0
120-121	303.0
122-123	306.0
124-125	350.0
126-127	416.0
128-129	463.0
130-131	544.0
132-133	704.0
134-135	916.0
136-137	1418.0
138-139	3006.0
140-141	6286.0
142-143	8909.0
144-145	59779.0
146-147	763027.0
148-149	797377.0
150-151	2.0561734E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.08853964805017
#Duplication Level	Percentage of total
1	25.79205975242271
2	11.60850447261171
3	7.621988362298891
4	5.4182906020993045
5	4.1057338819510205
6	3.372946120970013
7	2.6729376982946893
8	2.301438764590443
9	1.9473527148634742
>10	22.056131393365135
>50	5.4068133768849735
>100	6.073017117959865
>500	0.8040446944705916
>1k	0.728464381409073
>5k	0.09027666580804511
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.5027656662136735E-6	4.5027656662136735E-6	0.0	0.0	0.0015354430921788627	0.0
2	1.350829699864102E-5	3.602212532970939E-5	0.0	9.005531332427347E-6	0.005605943254436024	0.0
3	4.5027656662136736E-5	3.602212532970939E-5	0.0	9.005531332427347E-6	0.0071729057062783815	0.0
4	1.0356361032291449E-4	3.602212532970939E-5	0.0	9.005531332427347E-6	0.008514729874810056	0.0
5	1.0806637598912816E-4	3.602212532970939E-5	4.5027656662136735E-6	9.005531332427347E-6	0.010230283593637465	0.0
6	1.1256914165534183E-4	3.602212532970939E-5	4.5027656662136735E-6	9.005531332427347E-6	0.011801748811146038	0.0
7	1.350829699864102E-4	4.953042232835041E-5	9.005531332427347E-6	9.005531332427347E-6	0.013809982298277337	0.0
8	1.8011062664854695E-4	5.8535953660777756E-5	9.005531332427347E-6	9.005531332427347E-6	0.01905120153375005	0.0
9	1.8461339231476062E-4	7.204425065941878E-5	9.005531332427347E-6	9.005531332427347E-6	0.02460311160019151	0.0
10-11	2.386465803093247E-4	8.330116482495296E-5	9.005531332427347E-6	9.005531332427347E-6	0.03101279852604668	0.0
12-13	2.814228541383546E-4	8.55525476580598E-5	9.005531332427347E-6	9.005531332427347E-6	0.03697671165094668	0.0
14-15	3.196963623011708E-4	9.906084465670081E-5	9.005531332427347E-6	9.005531332427347E-6	0.04490157922348276	0.0
16-17	3.557184876308802E-4	1.0581499315602132E-4	9.005531332427347E-6	9.005531332427347E-6	0.05061108608824169	0.0
18-19	4.097516756254443E-4	1.2382605582087603E-4	9.005531332427347E-6	9.005531332427347E-6	0.05857197578610747	0.0
20-21	4.592820979537947E-4	1.3058020432019652E-4	9.005531332427347E-6	9.005531332427347E-6	0.06431975615902921	0.0
22-23	4.975556061166109E-4	1.8011062664854695E-4	9.005531332427347E-6	9.005531332427347E-6	0.07006978791478408	0.0
24-25	5.358291142794271E-4	1.913675408140811E-4	9.005531332427347E-6	9.005531332427347E-6	0.07522545460259873	0.0
26-27	5.718512396091365E-4	1.9812168931340163E-4	9.005531332427347E-6	9.005531332427347E-6	0.07896275010555609	0.0
28-29	6.844203812644784E-4	1.9812168931340163E-4	9.005531332427347E-6	9.005531332427347E-6	0.08286664793816334	0.0
30-31	8.735365392454526E-4	1.9812168931340163E-4	9.005531332427347E-6	9.005531332427347E-6	0.08764858507568227	0.0
32-33	0.0010153736577311833	2.2513828331068367E-4	9.005531332427347E-6	9.005531332427347E-6	0.09288980431115498	4.5027656662136735E-6
34-35	0.001134696947885846	2.3639519747621786E-4	1.1256914165534184E-5	9.005531332427347E-6	0.0958931490105195	4.5027656662136735E-6
36-37	0.0011752218388817687	2.4314934597553838E-4	1.350829699864102E-5	9.005531332427347E-6	0.09916215688419062	4.5027656662136735E-6
38-39	0.0012292550268763328	2.4314934597553838E-4	1.350829699864102E-5	9.005531332427347E-6	0.10237262880420098	4.5027656662136735E-6
40-41	0.001265277152206042	2.566576429741794E-4	1.575967983174786E-5	9.005531332427347E-6	0.10519811425975006	4.5027656662136735E-6
42-43	0.0012990478947026447	2.7241732280592723E-4	1.8011062664854694E-5	1.350829699864102E-5	0.10782998079165194	4.5027656662136735E-6
44-45	0.0013328186371992473	2.836742369714614E-4	1.8011062664854694E-5	1.350829699864102E-5	0.11000256522560004	4.5027656662136735E-6
46-47	0.0013755949110282773	3.2419912796738447E-4	1.8011062664854694E-5	1.350829699864102E-5	0.11227871326987104	4.5027656662136735E-6
48-49	0.0014228739505235209	3.3545604213291866E-4	1.8011062664854694E-5	1.350829699864102E-5	0.11471020672962644	4.5027656662136735E-6
50-51	0.0014836612870174053	3.422101906322392E-4	1.8011062664854694E-5	1.350829699864102E-5	0.11676797063908609	4.5027656662136735E-6
52-53	0.0015196834123471148	3.44461573465346E-4	1.8011062664854694E-5	1.350829699864102E-5	0.11870415987555796	4.5027656662136735E-6
54-55	0.0015962304286727472	3.5121572196466653E-4	1.8011062664854694E-5	1.350829699864102E-5	0.12043772465705023	4.5027656662136735E-6
56-57	0.0016367553196686702	3.6922678462952125E-4	1.8011062664854694E-5	1.350829699864102E-5	0.12238517080768765	4.5027656662136735E-6
58-59	0.0016750288278314866	3.7372955029573487E-4	1.8011062664854694E-5	1.350829699864102E-5	0.12423580749650147	4.5027656662136735E-6
60-61	0.0017268106329929437	3.8948923012748274E-4	1.8011062664854694E-5	1.350829699864102E-5	0.12555286645386896	9.005531332427347E-6
62-63	0.0017448216956577984	3.9624337862680326E-4	1.8011062664854694E-5	1.575967983174786E-5	0.12736072686885375	9.005531332427347E-6
64-65	0.0017583299926564396	4.0750029279233745E-4	1.8011062664854694E-5	1.8011062664854694E-5	0.12909879441601224	1.1256914165534184E-5
66-67	0.0018213687119834308	4.367682696227263E-4	2.026244549796153E-5	1.8011062664854694E-5	0.13041135060771353	1.350829699864102E-5
68-69	0.0018416311574813923	4.5027656662136734E-4	2.2513828331068368E-5	1.8011062664854694E-5	0.13176443169041072	1.575967983174786E-5
70-71	0.0018618936029793538	4.525279494544742E-4	2.2513828331068368E-5	1.8011062664854694E-5	0.1330364629911161	1.8011062664854694E-5
72-73	0.0018866588141435291	4.7504177778554256E-4	2.2513828331068368E-5	1.8011062664854694E-5	0.1338964912333629	1.8011062664854694E-5
74-75	0.0019114240253077044	4.908014576172904E-4	3.151935966349572E-5	1.8011062664854694E-5	0.1349163676567603	2.2513828331068368E-5
76-77	0.0019429433849712002	5.088125202821451E-4	3.602212532970939E-5	1.8011062664854694E-5	0.13609609226130828	2.2513828331068368E-5
78-79	0.0020082334871312983	5.448346456118545E-4	3.602212532970939E-5	1.8011062664854694E-5	0.1375212175946649	2.701659399728204E-5
80-81	0.002012736252797512	5.538401769442818E-4	4.052489099592306E-5	1.8011062664854694E-5	0.13882927102069997	2.9267976830388878E-5
82-83	0.0020307473154623666	5.628457082767092E-4	4.27762738290299E-5	2.2513828331068368E-5	0.14024313943989108	3.3770742496602556E-5
84-85	0.0020487583781272213	5.673484739429228E-4	4.7279039495243575E-5	2.2513828331068368E-5	0.14171329242990985	3.602212532970939E-5
86-87	0.0020712722064582896	5.786053881084571E-4	4.953042232835041E-5	2.2513828331068368E-5	0.14339057264057442	3.602212532970939E-5
88-89	0.0020870318862900375	5.853595366077776E-4	4.953042232835041E-5	2.2513828331068368E-5	0.14505209317140727	3.602212532970939E-5
90-91	0.0020937860347893583	6.281358104368074E-4	4.953042232835041E-5	2.701659399728204E-5	0.14597966289864728	3.602212532970939E-5
92-93	0.002096037417622465	6.303871932699142E-4	4.953042232835041E-5	2.701659399728204E-5	0.1468734618833907	3.602212532970939E-5
94-95	0.002109545714621106	6.32638576103021E-4	4.953042232835041E-5	2.701659399728204E-5	0.1478190426732956	4.27762738290299E-5
96-97	0.00212080262878664	6.438954902685553E-4	4.953042232835041E-5	2.701659399728204E-5	0.14889520366752065	4.7279039495243575E-5
98-99	0.0021253053944528537	6.529010216009826E-4	4.953042232835041E-5	2.701659399728204E-5	0.149935342536416	5.403318799456408E-5
100-101	0.0021320595429521745	6.529010216009826E-4	4.953042232835041E-5	2.701659399728204E-5	0.15083814705249185	6.529010216009827E-5
102-103	0.0021343109257852813	6.529010216009826E-4	4.953042232835041E-5	2.701659399728204E-5	0.1517432029514008	7.204425065941878E-5
104-105	0.002138813691451495	6.529010216009826E-4	5.178180516145724E-5	2.701659399728204E-5	0.1526054825764807	7.204425065941878E-5
106-107	0.002179338582447418	6.6190655293341E-4	5.403318799456408E-5	2.701659399728204E-5	0.15333493061440734	7.654701632563245E-5
108-109	0.0021838413481136314	6.866717640975851E-4	5.403318799456408E-5	2.701659399728204E-5	0.1540418648240029	7.654701632563245E-5
110-111	0.0021838413481136314	7.001800610962263E-4	6.0787336493884595E-5	2.701659399728204E-5	0.1548793792379186	7.654701632563245E-5
112-113	0.0021838413481136314	7.091855924286536E-4	6.303871932699143E-5	2.701659399728204E-5	0.1555975703616797	7.654701632563245E-5
114-115	0.0021883441137798454	7.114369752617604E-4	6.303871932699143E-5	2.701659399728204E-5	0.15641257094726438	7.879839915873928E-5
116-117	0.0021973496451122725	7.136883580948672E-4	6.75414849932051E-5	2.701659399728204E-5	0.15735590035433614	8.330116482495296E-5
118-119	0.002204103793611593	7.226938894272946E-4	6.75414849932051E-5	2.701659399728204E-5	0.1581348788145911	8.780393049116663E-5
120-121	0.002208606559277807	7.294480379266151E-4	6.75414849932051E-5	2.9267976830388878E-5	0.15894087386884337	9.455807899048714E-5
122-123	0.0022153607077771273	7.294480379266151E-4	6.75414849932051E-5	3.151935966349572E-5	0.15965906499260443	9.906084465670081E-5
124-125	0.0022153607077771273	7.294480379266151E-4	7.204425065941878E-5	3.151935966349572E-5	0.16051008770351882	1.0356361032291449E-4
126-127	0.0022288690047757684	7.339508035928288E-4	7.654701632563245E-5	3.151935966349572E-5	0.16142189775092708	1.0806637598912816E-4
128-129	0.0022401259189413023	7.407049520921493E-4	7.654701632563245E-5	3.151935966349572E-5	0.1623854896034968	1.1256914165534183E-4
130-131	0.0022648911301054774	7.519618662576834E-4	8.104978199184612E-5	3.151935966349572E-5	0.16329955103373817	1.1932329015466235E-4
132-133	0.0022851535756034393	7.564646319238971E-4	8.55525476580598E-5	3.151935966349572E-5	0.16407627811116005	1.2607743865398287E-4
134-135	0.0023144215524338284	7.609673975901108E-4	8.55525476580598E-5	3.151935966349572E-5	0.16484399965724947	1.2607743865398287E-4
136-137	0.0023234270837662555	7.654701632563244E-4	8.55525476580598E-5	3.151935966349572E-5	0.1657085306651625	1.2607743865398287E-4
138-139	0.0023234270837662555	7.722243117556449E-4	8.55525476580598E-5	3.151935966349572E-5	0.1665595533760769	1.3058020432019654E-4
>>END_MODULE
