##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22697696
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.20781911080314	37.0	35.0	38.0	33.0	39.0
2	35.50978588311342	35.0	34.0	38.0	33.0	39.0
3	35.647397075015895	36.0	35.0	38.0	33.0	39.0
4	35.734488425609364	36.0	35.0	38.0	33.0	39.0
5	35.6550866220078	36.0	35.0	38.0	33.0	39.0
6	35.58313526624024	36.0	35.0	37.0	33.0	39.0
7	35.544895658132	35.0	34.0	38.0	33.0	39.0
8	35.71486176394291	36.0	35.0	38.0	33.0	39.0
9	35.688873883939586	36.0	35.0	38.0	33.0	39.0
10-14	35.61313771230349	36.0	35.0	37.8	33.0	39.0
15-19	35.61903922759385	36.0	35.0	38.0	33.0	39.0
20-24	35.60081172115442	36.0	35.0	38.0	33.0	39.0
25-29	35.58320702682774	36.0	35.0	38.0	33.0	39.0
30-34	35.55679335911451	36.0	35.0	38.0	33.0	39.0
35-39	35.531637360902174	36.0	35.0	38.0	33.0	39.0
40-44	35.51292826373215	36.0	35.0	38.0	33.0	39.0
45-49	35.478776938417006	36.0	34.2	38.0	33.0	39.0
50-54	35.4548468796128	36.0	34.0	38.0	33.0	39.0
55-59	35.422970578159116	36.0	34.0	38.0	33.0	39.0
60-64	35.38220079137992	36.0	34.0	38.0	32.8	39.0
65-69	35.34302865020966	36.0	34.0	38.0	32.0	39.0
70-74	35.31032274601871	36.0	34.0	38.0	32.0	39.0
75-79	35.262055729900645	36.0	34.0	38.0	32.0	39.0
80-84	35.220159915872216	36.0	34.0	38.0	32.0	39.0
85-89	35.17228004048029	36.0	34.0	38.0	32.0	39.0
90-94	35.11281568855014	36.0	34.0	38.0	32.0	39.0
95-99	35.045129242444126	35.8	34.0	37.2	32.0	39.0
100-104	34.99188006199025	35.6	34.0	37.0	31.6	39.0
105-109	34.92142118672628	35.0	34.0	37.0	31.0	39.0
110-114	34.85438915521571	35.0	34.0	37.0	31.0	39.0
115-119	34.7780898527408	35.0	34.0	37.0	31.0	39.0
120-124	34.69860280945794	35.0	34.0	37.0	30.6	39.0
125-129	34.63015749699207	35.0	34.0	37.0	30.0	39.0
130-134	34.53350880521709	35.0	34.0	37.0	30.0	39.0
135-139	34.43568837045348	35.0	34.0	37.0	30.0	39.0
140-144	34.33401065429164	35.0	34.0	37.0	29.8	39.0
145-149	34.299241941649335	35.0	34.0	37.0	29.2	39.0
150	35.03623044644985	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	156.0
22	1399.0
23	7617.0
24	27246.0
25	68239.0
26	131974.0
27	222404.0
28	318792.0
29	414316.0
30	527160.0
31	687525.0
32	930827.0
33	1335946.0
34	2102493.0
35	3860209.0
36	1.0539568E7
37	1521810.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.256058412272324	18.22258964081641	16.75945875739987	30.76189318951139
2	23.874565236690334	25.167806686978565	33.06259512254438	17.895032953786718
3	20.34330709160965	29.176745516373114	30.06012152070413	20.419825871313105
4	23.70740288826685	33.75508834707665	22.373959117875188	20.163549646781316
5	25.11251362252803	35.086169098396596	22.70573630028352	17.095580978791855
6	19.035438663025534	38.96115270906792	23.099366561258023	18.904042066648525
7	20.423377792326487	20.705882249276854	38.2101750860605	20.660564872336156
8	21.789296225894304	23.255991412343853	28.67406580271697	26.280646559044875
9	23.75094475452243	23.065068942022528	28.990503220701484	24.19348308275356
10-14	23.223840785890502	29.103346578184986	25.88223911125415	21.790573524670364
15-19	23.054754103676427	28.257386124124668	27.24743603932311	21.440423732875797
20-24	23.08095147630843	28.356192628538157	27.08742332261389	21.47543257253952
25-29	23.20024132141202	28.313434353102927	26.99842245660901	21.487901868876047
30-34	23.193476553743604	28.09305666971661	27.175187296543225	21.53827947999656
35-39	23.264363924867087	28.102369509222434	27.127901439864203	21.505365126046275
40-44	23.404411825803038	27.86601360654415	27.239064504512395	21.490510063140416
45-49	23.404673320146678	27.702777409654267	27.326050185886707	21.566499084312348
50-54	23.457263886671704	27.667478035369562	27.290608943662026	21.584649134296708
55-59	23.614358919953816	27.552200892989315	27.199247007273335	21.634193179783534
60-64	23.516332385292866	27.53126077339571	27.28304261732304	21.66936422398838
65-69	23.60482674668656	27.44347288204218	27.256674137324104	21.695026233947154
70-74	23.69306395016363	27.398004360923125	27.180285991804425	21.728645697108824
75-79	23.611022983942508	27.427892137984983	27.261881846777918	21.69920303129459
80-84	23.72588126205329	27.471264373893007	27.121134788039047	21.681719576014654
85-89	23.732563564300023	27.425648547578486	27.184176528638492	21.657611359483
90-94	23.681757603364908	27.37148627297536	27.233192767583542	21.71356335607619
95-99	23.737292081054132	27.455988727057225	27.25619803629005	21.550521155598588
100-104	23.909373935786995	27.410942373057956	27.07403973146483	21.60564395969022
105-109	23.69445491745187	27.53855353063378	27.180050186132647	21.586941365781705
110-114	23.71947025740203	27.531492900983928	27.118667114370034	21.630369727244016
115-119	23.785098789149938	27.525487776935687	27.150819571003048	21.53859386291133
120-124	23.74720058097256	27.444946686942263	27.30819386504601	21.49965886703917
125-129	23.718340261481064	27.542694376578368	27.26603644855591	21.47292891338466
130-134	23.78997762302296	27.452611448782342	27.319311868470532	21.438099059724163
135-139	23.68976132347008	27.448667432199684	27.42747849836235	21.434092745967884
140-144	23.703271480739687	27.590548925901505	27.378883304861905	21.327296288496907
145-149	23.467611553678893	27.89364028046148	27.154749864236727	21.4839983016229
150	23.724290302759815	26.5722448969465	27.494175934166993	22.20928886612669
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	13.5
3	33.5
4	46.5
5	47.5
6	42.0
7	35.5
8	30.5
9	29.0
10	20.5
11	17.0
12	21.5
13	16.5
14	19.5
15	44.0
16	69.5
17	106.5
18	195.0
19	363.0
20	564.5
21	921.5
22	1475.0
23	2433.0
24	4044.5
25	6623.0
26	10155.0
27	15904.0
28	26181.0
29	40002.5
30	55151.0
31	73412.5
32	99094.5
33	133160.5
34	177308.5
35	242103.5
36	338530.0
37	455568.5
38	592236.5
39	766307.5
40	970371.0
41	1161417.5
42	1331944.0
43	1490905.0
44	1605368.0
45	1653669.0
46	1643547.5
47	1583779.5
48	1483716.0
49	1344062.5
50	1163838.5
51	972128.5
52	790386.5
53	627756.0
54	488660.0
55	367236.0
56	270203.0
57	200670.5
58	147715.5
59	106597.5
60	74219.5
61	49817.5
62	34546.0
63	24886.5
64	17513.5
65	12753.5
66	9627.5
67	7185.5
68	6407.5
69	5035.5
70	2946.5
71	2108.0
72	1550.0
73	1180.5
74	940.5
75	519.0
76	190.5
77	71.0
78	20.0
79	2.0
80	1.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.4057335158599355E-6
3	0.0
4	4.4057335158599355E-6
5	0.0
6	0.0
7	4.4057335158599355E-6
8	4.4057335158599355E-6
9	4.4057335158599355E-6
10-14	0.09678074814289521
15-19	0.0
20-24	0.0
25-29	8.811467031719872E-7
30-34	0.0
35-39	0.0
40-44	8.811467031719872E-7
45-49	0.0
50-54	8.811467031719872E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	2.6434478426769285E-6
80-84	8.811527825787142E-7
85-89	0.0
90-94	0.0
95-99	4.405916331419759E-6
100-104	8.811989825993778E-7
105-109	0.0
110-114	0.0
115-119	8.812591352974325E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	8.990498463622708E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	1.0
64-65	2.0
66-67	4.0
68-69	5.0
70-71	11.0
72-73	14.0
74-75	8.0
76-77	35.0
78-79	30.0
80-81	42.0
82-83	63.0
84-85	69.0
86-87	77.0
88-89	124.0
90-91	126.0
92-93	114.0
94-95	131.0
96-97	165.0
98-99	157.0
100-101	158.0
102-103	180.0
104-105	161.0
106-107	206.0
108-109	216.0
110-111	205.0
112-113	222.0
114-115	242.0
116-117	254.0
118-119	284.0
120-121	300.0
122-123	324.0
124-125	330.0
126-127	415.0
128-129	481.0
130-131	610.0
132-133	793.0
134-135	1076.0
136-137	1478.0
138-139	3299.0
140-141	6591.0
142-143	9873.0
144-145	62789.0
146-147	776184.0
148-149	871120.0
150-151	2.0958726E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.338792292497146
#Duplication Level	Percentage of total
1	26.1321130043014
2	11.51036567278736
3	7.579416500992525
4	5.370944703338785
5	4.05322520939188
6	3.332448495032588
7	2.649443390367622
8	2.3329727030004603
9	1.9456749052581601
>10	22.02890995558827
>50	5.50801490537202
>100	5.94197090338369
>500	0.853540441892232
>1k	0.68086047059867
>5k	0.08009873869421247
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.4057335158599355E-6	0.0	0.0	0.0018107564750184336	0.0
2	8.811467031719871E-6	2.2028667579299678E-5	0.0	0.0	0.005441080892087021	0.0
3	5.7274535706179165E-5	2.6434401095159613E-5	0.0	0.0	0.007084419493502777	0.0
4	1.1454907141235833E-4	3.084013461101955E-5	0.0	0.0	0.00816382420488846	0.0
5	1.1454907141235833E-4	3.084013461101955E-5	0.0	0.0	0.009666179333796699	0.0
6	1.233605384440782E-4	3.084013461101955E-5	0.0	0.0	0.011221403264895256	0.0
7	1.3657773899165802E-4	6.16802692220391E-5	0.0	0.0	0.013195171880000507	0.0
8	1.718236071185375E-4	7.049173625375897E-5	0.0	0.0	0.017975392744708538	0.0
9	1.8063507415025737E-4	7.930320328547885E-5	0.0	0.0	0.023676411914231296	0.0
10-11	1.9825800821369712E-4	8.370893680133878E-5	0.0	0.0	0.02940606835160714	0.0
12-13	2.4011247661436647E-4	8.370893680133878E-5	0.0	0.0	0.03484714924369416	0.0
14-15	2.9958987907847566E-4	8.370893680133878E-5	0.0	0.0	0.042319273286592615	0.0
16-17	3.5025581451086484E-4	8.811467031719871E-5	2.2028667579299678E-6	0.0	0.04773171691082655	0.0
18-19	4.317618845542737E-4	1.035347376227085E-4	4.4057335158599355E-6	0.0	0.055111320549891936	0.0
20-21	4.934421537763128E-4	1.1454907141235833E-4	6.608600273789903E-6	0.0	0.06033431763294389	0.0
22-23	5.617310232721418E-4	1.2776627195993813E-4	1.3217200547579807E-5	0.0	0.06579963005936815	0.0
24-25	6.10194091946601E-4	1.4098347250751794E-4	1.3217200547579807E-5	0.0	0.0709433239391346	0.0
26-27	6.498456935893405E-4	1.4538920602337788E-4	1.3217200547579807E-5	0.0	0.07485561530121823	0.0
28-29	7.665976317596288E-4	1.4538920602337788E-4	1.3217200547579807E-5	0.0	0.07877010953005979	0.0
30-31	9.824785740367658E-4	1.4979493953923783E-4	1.3217200547579807E-5	0.0	0.0835260988604306	0.0
32-33	0.0011565050479132332	1.6741787360267756E-4	1.3217200547579807E-5	0.0	0.08806400438176633	0.0
34-35	0.0013283286550317707	1.718236071185375E-4	1.3217200547579807E-5	0.0	0.0907448932261671	0.0
36-37	0.0013922117910117396	1.718236071185375E-4	1.7622934063439742E-5	0.0	0.09407122203064135	0.0
38-39	0.001445080593202059	1.718236071185375E-4	2.2028667579299678E-5	0.0	0.09721911862772328	0.0
40-41	0.0014627035272654987	1.7402647387646745E-4	2.2028667579299678E-5	0.0	0.09980748706829098	0.0
42-43	0.0014825293280868682	1.8283794090818734E-4	2.2028667579299678E-5	0.0	0.10273509698957992	0.0
44-45	0.001513369462697888	1.8944654118197723E-4	2.2028667579299678E-5	0.0	0.10506573001946981	0.0
46-47	0.0015486153308247675	2.3130100958264663E-4	2.2028667579299678E-5	0.0	0.10754615798889897	4.4057335158599355E-6
48-49	0.0016058898665309465	2.5553254391987625E-4	2.2028667579299678E-5	0.0	0.10998693435668536	4.4057335158599355E-6
50-51	0.0016675701357529856	2.599382774357362E-4	2.2028667579299678E-5	0.0	0.11199154310640164	4.4057335158599355E-6
52-53	0.0017116274709115852	2.6654687770952615E-4	2.2028667579299678E-5	0.0	0.11407545505940339	1.1014333789649839E-5
54-55	0.0017689020066177642	2.7976407825710593E-4	2.2028667579299678E-5	0.0	0.11589061726793767	1.3217200547579807E-5
56-57	0.0018415966096294533	2.929812788046857E-4	2.2028667579299678E-5	0.0	0.11780050274706297	1.7622934063439742E-5
58-59	0.0018988711453356323	2.973870123205457E-4	2.2028667579299678E-5	0.0	0.11968835955860894	1.7622934063439742E-5
60-61	0.0019539428142838814	3.106042128681255E-4	2.2028667579299678E-5	0.0	0.12099686241281934	1.7622934063439742E-5
62-63	0.0019781743486211112	3.260242801736352E-4	2.2028667579299678E-5	0.0	0.12288471922436534	1.7622934063439742E-5
64-65	0.0020024058829583406	3.3703861396328506E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12465362123098309	1.7622934063439742E-5
66-67	0.002116954954370699	3.6127014830051473E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12598635561953073	1.7622934063439742E-5
68-69	0.0021522008224975787	3.678787485743046E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12736975594351074	1.7622934063439742E-5
70-71	0.002178635223592738	3.744873488480945E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12867165019744736	2.2028667579299678E-5
72-73	0.002205069624687898	3.832988158798144E-4	2.2028667579299678E-5	6.608600273789903E-6	0.12953737683331384	3.084013461101955E-5
74-75	0.002220489691993408	3.9431314966946424E-4	2.8637267853089583E-5	8.811467031719871E-6	0.13059034714360435	3.084013461101955E-5
76-77	0.002257938426878217	4.2955901779634376E-4	3.304300136894952E-5	8.811467031719871E-6	0.13172922925745414	3.084013461101955E-5
78-79	0.0023174158293423263	4.3837048482806365E-4	3.5245868126879484E-5	8.811467031719871E-6	0.1331588897833507	3.084013461101955E-5
80-81	0.002339444496921626	4.537905521335734E-4	3.744873488480946E-5	1.1014333789649839E-5	0.13455330444112037	3.084013461101955E-5
82-83	0.0023482559639533456	4.78022086470803E-4	4.405733515859936E-5	1.3217200547579807E-5	0.13611513697249272	3.084013461101955E-5
84-85	0.0023702846315326455	4.80224953228733E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.13763291216870646	3.084013461101955E-5
86-87	0.002398921899385735	4.890364202604529E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.13942604570966147	3.304300136894952E-5
88-89	0.0024231534337229646	5.000507540501027E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.14106277571080342	3.744873488480946E-5
90-91	0.0024341677675126143	5.066593543238926E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.14207829728620913	3.965160164273942E-5
92-93	0.002440776367786404	5.110650878397525E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.1430453557929404	3.965160164273942E-5
94-95	0.0024495878348181244	5.198765548714724E-4	5.2868802190319226E-5	1.7622934063439742E-5	0.14410713757026264	4.185446840066939E-5
96-97	0.002460602168607774	5.242822883873323E-4	5.2868802190319226E-5	1.7622934063439742E-5	0.1450147186745298	4.8463068674459294E-5
98-99	0.0024760222359132837	5.330937554190523E-4	5.2868802190319226E-5	1.9825800821369708E-5	0.14593992271286038	5.066593543238926E-5
100-101	0.0024804279694291436	5.374994889349122E-4	5.2868802190319226E-5	2.2028667579299678E-5	0.1468298808830641	5.50716689482492E-5
102-103	0.002484833702945004	5.46310955966632E-4	5.2868802190319226E-5	2.2028667579299678E-5	0.14771102758623605	5.947740246410913E-5
104-105	0.002484833702945004	5.573252897562819E-4	5.50716689482492E-5	2.2028667579299678E-5	0.14852829115342808	6.16802692220391E-5
106-107	0.0025068623705243034	5.727453570617916E-4	5.7274535706179165E-5	2.2028667579299678E-5	0.14930590311897735	6.16802692220391E-5
108-109	0.002515673837556023	5.859625576093714E-4	5.7274535706179165E-5	2.2028667579299678E-5	0.15011876095265353	6.608600273789904E-5
110-111	0.0025288910381036033	5.969768913990214E-4	5.7274535706179165E-5	2.2028667579299678E-5	0.1510946309264165	7.049173625375897E-5
112-113	0.002535499638377393	5.991797581569513E-4	6.16802692220391E-5	2.2028667579299678E-5	0.1518876629592713	7.049173625375897E-5
114-115	0.002539905371893253	6.013826249148813E-4	6.608600273789904E-5	2.2028667579299678E-5	0.15282388133139152	7.049173625375897E-5
116-117	0.002542108238651183	6.057883584307412E-4	6.8288869495829E-5	2.2028667579299678E-5	0.1538900688422296	7.710033652754889E-5
118-119	0.0025509197056829028	6.079912251886711E-4	7.049173625375897E-5	2.2028667579299678E-5	0.15467649227481062	7.930320328547885E-5
120-121	0.0025575283059566926	6.079912251886711E-4	7.049173625375897E-5	2.2028667579299678E-5	0.1555488275109509	8.811467031719871E-5
122-123	0.0025597311727146225	6.10194091946601E-4	7.049173625375897E-5	2.2028667579299678E-5	0.15635727961111118	9.252040383305864E-5
124-125	0.0025597311727146225	6.21208425736251E-4	7.489746976961891E-5	2.2028667579299678E-5	0.1571943689791246	1.0133187086477852E-4
126-127	0.0025707455065042727	6.432370933155507E-4	7.489746976961891E-5	2.2028667579299678E-5	0.15814160168503447	1.0133187086477852E-4
128-129	0.0025861655738097823	6.498456935893405E-4	7.489746976961891E-5	2.2028667579299678E-5	0.15906900859012296	1.0573760438063845E-4
130-131	0.002592774174083572	6.564542938631304E-4	7.489746976961891E-5	2.2028667579299678E-5	0.16011977603365557	1.0573760438063845E-4
132-133	0.0026302229089683812	6.608600273789903E-4	7.489746976961891E-5	2.6434401095159613E-5	0.1609436482011214	1.101433378964984E-4
134-135	0.0026632659103373313	6.608600273789903E-4	7.489746976961891E-5	2.6434401095159613E-5	0.1617256659001865	1.101433378964984E-4
136-137	0.002669874510611121	6.740772279265701E-4	7.710033652754889E-5	2.6434401095159613E-5	0.16256055240144196	1.101433378964984E-4
138-139	0.002674280244126981	6.8729442847415E-4	7.930320328547885E-5	2.8637267853089583E-5	0.16328309269804303	1.1675193817028829E-4
>>END_MODULE
