##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24030269
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.14012202693195	37.0	35.0	38.0	33.0	39.0
2	35.468793087584665	35.0	34.0	38.0	33.0	39.0
3	35.59280543218222	36.0	35.0	37.0	33.0	39.0
4	35.68002330727134	36.0	35.0	38.0	33.0	39.0
5	35.61046287080681	36.0	35.0	38.0	33.0	39.0
6	35.54104550390176	36.0	35.0	37.0	33.0	39.0
7	35.5178899162552	35.0	34.0	38.0	33.0	39.0
8	35.65191775422905	36.0	35.0	38.0	33.0	39.0
9	35.64086860617332	36.0	35.0	38.0	33.0	39.0
10-14	35.55900057548253	36.0	35.0	37.8	33.0	39.0
15-19	35.567554021138925	36.0	35.0	38.0	33.0	39.0
20-24	35.55069121365225	36.0	35.0	38.0	33.0	39.0
25-29	35.530766010151616	36.0	35.0	38.0	33.0	39.0
30-34	35.50536562865776	36.0	34.4	38.0	33.0	39.0
35-39	35.47968878750379	36.0	34.0	38.0	33.0	39.0
40-44	35.46060846010505	36.0	34.0	38.0	33.0	39.0
45-49	35.421254044222316	36.0	34.0	38.0	33.0	39.0
50-54	35.40268571275669	36.0	34.0	38.0	32.6	39.0
55-59	35.363861211874074	36.0	34.0	38.0	32.0	39.0
60-64	35.323322040215196	36.0	34.0	38.0	32.0	39.0
65-69	35.281475568934844	36.0	34.0	38.0	32.0	39.0
70-74	35.24640143434972	36.0	34.0	38.0	32.0	39.0
75-79	35.19300916706302	36.0	34.0	38.0	32.0	39.0
80-84	35.14776283649282	36.0	34.0	38.0	32.0	39.0
85-89	35.100273521503595	36.0	34.0	38.0	32.0	39.0
90-94	35.04031994706785	36.0	34.0	37.6	32.0	39.0
95-99	34.96772584208503	35.2	34.0	37.2	31.0	39.0
100-104	34.90930380492842	35.0	34.0	37.0	31.0	39.0
105-109	34.839677971569486	35.0	34.0	37.0	31.0	39.0
110-114	34.759840396052226	35.0	34.0	37.0	31.0	39.0
115-119	34.67975082396333	35.0	34.0	37.0	30.2	39.0
120-124	34.593288752180634	35.0	34.0	37.0	30.0	39.0
125-129	34.52393941179538	35.0	34.0	37.0	30.0	39.0
130-134	34.42333913628895	35.0	34.0	37.0	30.0	39.0
135-139	34.32135216967512	35.0	34.0	37.0	29.4	39.0
140-144	34.220135448595336	35.0	34.0	37.0	29.0	39.0
145-149	34.180179310143956	35.0	34.0	37.0	29.0	39.0
150	34.96333580527309	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	138.0
22	1399.0
23	8025.0
24	29225.0
25	74765.0
26	146514.0
27	247034.0
28	356601.0
29	463210.0
30	594102.0
31	779208.0
32	1061000.0
33	1516193.0
34	2352519.0
35	4151912.0
36	1.0346913E7
37	1901498.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.337350489085246	19.307961138512432	15.514965729264205	31.839722643138117
2	24.358404991571465	25.980933712433004	32.05733286037426	17.603328435621275
3	20.2037605155398	29.503822866069456	29.7106411917403	20.58177542665045
4	23.586474358088726	34.01152246824713	21.900038734482692	20.501964439181453
5	25.59939716030645	34.95935480372692	21.951522889735443	17.489725146231198
6	19.365305482015202	39.163494174784304	22.131167154225366	19.340033188975124
7	20.6034023173024	21.21068224413135	36.876520192096066	21.309395246470192
8	21.62193530259421	23.915596779861133	28.096012079432487	26.366455838112167
9	23.578483216893364	23.580389146769708	28.013607007663044	24.82752062867388
10-14	23.088950322338984	29.16914138735629	25.29621454759006	22.445693742714663
15-19	23.199547144463413	27.963541413990544	26.794391749651798	22.042519691894242
20-24	23.06713420478148	28.000752717333295	26.718552339135282	22.213560738749948
25-29	23.20474584121173	27.630619762355717	26.695355983700026	22.46927841273253
30-34	23.06465899320561	27.326057814833447	26.90621981801369	22.70306337394725
35-39	23.348967920417373	27.06436120211555	26.90983609047406	22.676834786993023
40-44	23.505418936425556	26.72597464472828	26.98062764091405	22.787978777932118
45-49	23.424111871974667	26.57799838325569	27.090745877056072	22.90714386771357
50-54	23.5734776597545	26.547912993365376	26.879609660293184	22.99899968658694
55-59	23.66579999583026	26.491519508166974	26.91081319147946	22.93186730452331
60-64	23.421751320567832	26.445035854114124	26.979704862893584	23.153507962424456
65-69	23.574606533911695	26.39886317366117	26.923042959498417	23.103487332928715
70-74	23.785103169884323	26.336836008914194	26.70996307963161	23.168097741569873
75-79	23.641219714876744	26.464871198326406	26.902999175330716	22.990909911466133
80-84	23.909962916921216	26.43936618203022	26.622061249630434	23.028609651418126
85-89	23.73353434094817	26.519494622551925	26.775672965025116	22.971298071474788
90-94	23.699660039397887	26.33924754402408	26.780240106169202	23.180852310408834
95-99	23.70993944943901	26.552912179518323	26.82203059274374	22.915117778298928
100-104	23.952401200015938	26.289887750257762	26.784137050356804	22.973573999369496
105-109	23.761138278060816	26.481576453419603	26.75559419279785	23.001691075721734
110-114	23.85977253830364	26.58474640148956	26.646754956639292	22.908726103567503
115-119	23.967368474568044	26.59585701202675	26.784125160471984	22.652649352933217
120-124	23.863147150121566	26.67771204927592	26.97719058960903	22.48195021099348
125-129	23.891751789332545	26.776983747114365	26.792466308718165	22.53879815483492
130-134	24.060126354068444	26.771657521982213	26.73406864464048	22.434147479308866
135-139	23.877084228464106	26.8411470991111	26.919772497271804	22.36199617515299
140-144	23.880010907408668	26.963634701507154	26.799128893438663	22.357225497645523
145-149	23.66382719874746	27.327228709415667	26.525764817672897	22.483179274163977
150	23.99608831545259	26.08237269412537	26.757581189058648	23.163957801363388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.0
2	13.0
3	28.0
4	50.5
5	63.0
6	53.0
7	45.0
8	34.5
9	24.0
10	24.0
11	20.0
12	17.0
13	25.0
14	30.0
15	39.0
16	64.5
17	119.0
18	227.0
19	391.5
20	630.5
21	997.5
22	1453.5
23	2243.5
24	3713.0
25	5835.0
26	9022.0
27	13910.5
28	20490.0
29	28962.0
30	40542.5
31	57740.0
32	83975.0
33	114365.0
34	150520.5
35	209494.0
36	295751.0
37	393595.5
38	509183.5
39	662213.5
40	844267.5
41	1034053.5
42	1224918.5
43	1401350.0
44	1533321.0
45	1613199.0
46	1634614.0
47	1587273.5
48	1518798.5
49	1455900.5
50	1341832.0
51	1175638.5
52	1000541.0
53	833283.0
54	681866.0
55	563828.5
56	465272.5
57	383071.5
58	321000.5
59	261710.5
60	198627.5
61	136091.5
62	85375.0
63	51840.0
64	31689.0
65	16921.0
66	10110.5
67	5704.5
68	3263.5
69	2166.5
70	1605.5
71	1403.5
72	1243.5
73	1044.0
74	849.5
75	511.0
76	156.5
77	53.0
78	12.5
79	4.5
80	4.5
81	3.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.161418251289655E-6
3	0.0
4	4.161418251289655E-6
5	0.0
6	0.0
7	0.0
8	4.161418251289655E-6
9	1.664567300515862E-5
10-14	0.10628178985428753
15-19	3.329134601031724E-6
20-24	0.0
25-29	8.32283650257931E-7
30-34	0.0
35-39	0.0
40-44	0.0
45-49	1.664567300515862E-6
50-54	1.664567300515862E-6
55-59	0.0
60-64	8.32283650257931E-7
65-69	0.0
70-74	0.0
75-79	1.664569350898768E-6
80-84	8.322858461102805E-7
85-89	0.0
90-94	0.0
95-99	4.993784943333899E-6
100-104	1.664612631781452E-6
105-109	0.0
110-114	0.0
115-119	8.323451385038541E-7
120-124	0.0
125-129	8.323957782217961E-7
130-134	0.0
135-139	0.0
140-144	8.330335870228829E-7
145-149	8.486604378724633E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	1.0
68-69	7.0
70-71	1.0
72-73	3.0
74-75	11.0
76-77	12.0
78-79	8.0
80-81	18.0
82-83	23.0
84-85	26.0
86-87	43.0
88-89	45.0
90-91	52.0
92-93	50.0
94-95	59.0
96-97	71.0
98-99	106.0
100-101	121.0
102-103	104.0
104-105	116.0
106-107	140.0
108-109	156.0
110-111	142.0
112-113	176.0
114-115	184.0
116-117	195.0
118-119	221.0
120-121	252.0
122-123	334.0
124-125	356.0
126-127	401.0
128-129	500.0
130-131	560.0
132-133	790.0
134-135	1003.0
136-137	1683.0
138-139	4531.0
140-141	8826.0
142-143	10084.0
144-145	60976.0
146-147	796843.0
148-149	969516.0
150-151	2.2171522E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.21450264704313
#Duplication Level	Percentage of total
1	22.578144315889297
2	9.438315201198488
3	6.019977299366289
4	4.219225782788414
5	3.122601811601665
6	2.4430540953560365
7	2.046258026191501
8	1.7930704775608142
9	1.4925670942824312
>10	18.276900321703135
>50	6.4983462662673075
>100	13.075051936608004
>500	3.651849419577432
>1k	4.426684289123576
>5k	0.6770481074783827
>10k+	0.24090555500736982
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.663307680825379E-4	0.0	0.0	0.0	0.00325006765425722	0.0
2	0.0013940751141820343	4.161418251289655E-6	0.0	0.0	0.012384380715838013	0.0
3	0.0020557406161370896	4.161418251289655E-6	0.0	0.0	0.01663318875040475	0.0
4	0.005168481468101751	4.161418251289655E-6	0.0	0.0	0.02256320975849251	0.0
5	0.005397359471922682	8.32283650257931E-6	0.0	4.161418251289655E-6	0.027132446998408548	0.0
6	0.006654107783812158	8.32283650257931E-6	0.0	8.32283650257931E-6	0.0339904642765339	0.0
7	0.008813883856231488	1.2484254753868965E-5	0.0	8.32283650257931E-6	0.04455214379830704	0.0
8	0.015476314476546226	1.664567300515862E-5	4.161418251289655E-6	8.32283650257931E-6	0.06035304889845386	0.0
9	0.016088042959485804	2.496850950773793E-5	4.161418251289655E-6	8.32283650257931E-6	0.0724835831009632	0.0
10-11	0.016666480096415068	2.496850950773793E-5	4.161418251289655E-6	8.32283650257931E-6	0.12151965506503484	0.0
12-13	0.017269885742852067	2.704921863338276E-5	4.161418251289655E-6	8.32283650257931E-6	0.1479800330158601	0.0
14-15	0.019691831165102647	3.329134601031724E-5	4.161418251289655E-6	8.32283650257931E-6	0.1598858506327998	0.0
16-17	0.022509111321225742	3.329134601031724E-5	4.161418251289655E-6	8.32283650257931E-6	0.16800061622281465	0.0
18-19	0.028428728783685277	3.329134601031724E-5	4.161418251289655E-6	8.32283650257931E-6	0.1759260372823958	0.0
20-21	0.03380528116435151	3.7452764261606895E-5	4.161418251289655E-6	8.32283650257931E-6	0.18158556610414972	0.0
22-23	0.03603163992879148	4.1614182512896545E-5	4.161418251289655E-6	8.32283650257931E-6	0.18694131139355952	0.0
24-25	0.04067370198810509	4.1614182512896545E-5	4.161418251289655E-6	8.32283650257931E-6	0.192515531141162	0.0
26-27	0.0446707442184688	4.993701901547586E-5	4.161418251289655E-6	8.32283650257931E-6	0.19700154001605225	0.0
28-29	0.05403393528387052	4.993701901547586E-5	4.161418251289655E-6	1.0403545628224136E-5	0.2007593006969668	0.0
30-31	0.07526757191107598	5.409843726676551E-5	8.32283650257931E-6	1.2484254753868965E-5	0.20512462844256965	0.0
32-33	0.09533185000966907	5.409843726676551E-5	8.32283650257931E-6	1.664567300515862E-5	0.20899890883452032	0.0
34-35	0.1109017963968693	5.617914639241034E-5	8.32283650257931E-6	2.0807091256448273E-5	0.21257356711237815	4.161418251289655E-6
36-37	0.11373572222599757	6.0340564643699996E-5	8.32283650257931E-6	2.496850950773793E-5	0.21472918176654618	4.161418251289655E-6
38-39	0.1164115141615768	6.450198289498964E-5	8.32283650257931E-6	2.496850950773793E-5	0.21674330820017038	4.161418251289655E-6
40-41	0.11801574089744896	7.074411027192413E-5	8.32283650257931E-6	2.9129927759027584E-5	0.21856184797598394	4.161418251289655E-6
42-43	0.1183278472662957	7.906694677450345E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22033877356928463	4.161418251289655E-6
44-45	0.11903736907814058	8.114765590014827E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22182856130324632	6.2421273769344824E-6
46-47	0.12038358788243278	8.947049240272758E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22332042974633368	8.32283650257931E-6
48-49	0.12157375350230162	9.571261977966206E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22469369776925927	8.32283650257931E-6
50-51	0.12243100566206729	9.571261977966206E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22607945004693872	8.32283650257931E-6
52-53	0.12326328931232522	9.987403803095172E-5	8.32283650257931E-6	2.9129927759027584E-5	0.2273320369405769	8.32283650257931E-6
54-55	0.12370648035608756	1.0403545628224136E-4	8.32283650257931E-6	2.9129927759027584E-5	0.2285201218513201	8.32283650257931E-6
56-57	0.1245346025880942	1.1860042016175517E-4	8.32283650257931E-6	2.9129927759027584E-5	0.22978727370883778	8.32283650257931E-6
58-59	0.12470730144552272	1.3108467491562413E-4	1.0403545628224136E-5	2.9129927759027584E-5	0.23089837238193214	8.32283650257931E-6
60-61	0.12496530937710268	1.3940751141820344E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23183053007022103	1.2484254753868965E-5
62-63	0.12523372085431086	1.456496387951379E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23283967399615876	1.664567300515862E-5
64-65	0.12526493149119555	1.518917661720724E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23379471948482972	1.664567300515862E-5
66-67	0.12532735276496487	1.6645673005158618E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23462492242596203	1.664567300515862E-5
68-69	0.12535856340184956	1.68537439177231E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23543431827583786	1.664567300515862E-5
70-71	0.1254709216946344	1.7477956655416552E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23629365114472917	1.664567300515862E-5
72-73	0.12552918155015244	1.7894098480545517E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.236944913101056	1.664567300515862E-5
74-75	0.12555206935053453	1.8518311218238963E-4	1.2484254753868965E-5	3.7452764261606895E-5	0.2377751160421883	1.664567300515862E-5
76-77	0.1255832799874192	1.9350594868496895E-4	1.2484254753868965E-5	3.7452764261606895E-5	0.23859075401944108	1.664567300515862E-5
78-79	0.12562697487905775	2.0807091256448273E-4	1.2484254753868965E-5	3.7452764261606895E-5	0.23959781723625317	1.8726382130803444E-5
80-81	0.1256706697706963	2.1015162169012755E-4	1.664567300515862E-5	3.7452764261606895E-5	0.2404446658503906	2.496850950773793E-5
82-83	0.12569771898932966	2.205551673183517E-4	1.664567300515862E-5	3.953347338725172E-5	0.2413123215557845	2.496850950773793E-5
84-85	0.12572892962621435	2.351201311978655E-4	1.664567300515862E-5	4.1614182512896545E-5	0.24228609342658627	3.121063688467241E-5
86-87	0.1257643016813503	2.3928154944915516E-4	2.0807091256448273E-5	4.1614182512896545E-5	0.24318912118711614	3.7452764261606895E-5
88-89	0.12578302806348113	2.4136225857479998E-4	2.0807091256448273E-5	4.1614182512896545E-5	0.24418786156742567	4.1614182512896545E-5
90-91	0.1258100772821145	2.538465133286689E-4	2.0807091256448273E-5	4.1614182512896545E-5	0.24491194834315005	4.1614182512896545E-5
92-93	0.125824642245994	2.580079315799586E-4	2.0807091256448273E-5	4.3694891638541374E-5	0.24558609809985898	4.1614182512896545E-5
94-95	0.1258620950102556	2.642500589568931E-4	2.0807091256448273E-5	5.201772814112068E-5	0.24627273211132178	4.1614182512896545E-5
96-97	0.12587457926500947	2.746536045851172E-4	2.28878003820931E-5	5.409843726676551E-5	0.2469614468319102	4.1614182512896545E-5
98-99	0.1258849828106377	2.746536045851172E-4	2.496850950773793E-5	5.409843726676551E-5	0.24754196467796513	4.993701901547586E-5
100-101	0.1258995477745172	2.746536045851172E-4	2.496850950773793E-5	5.409843726676551E-5	0.24809335259626097	4.993701901547586E-5
102-103	0.1258995477745172	2.746536045851172E-4	2.496850950773793E-5	5.409843726676551E-5	0.24868427398794413	4.993701901547586E-5
104-105	0.12593283912052755	2.746536045851172E-4	2.9129927759027584E-5	5.409843726676551E-5	0.24938131154503512	4.993701901547586E-5
106-107	0.1259598883391609	2.7673431371076206E-4	2.9129927759027584E-5	5.409843726676551E-5	0.24993269946333102	5.201772814112068E-5
108-109	0.12598485684866867	2.8713785933898617E-4	3.329134601031724E-5	5.409843726676551E-5	0.25047368383599866	5.409843726676551E-5
110-111	0.1260514395406893	2.89218568464631E-4	3.329134601031724E-5	5.409843726676551E-5	0.2510687666459331	6.0340564643699996E-5
112-113	0.12606600450456878	2.912992775902758E-4	3.329134601031724E-5	5.409843726676551E-5	0.25171378647488296	6.658269202063447E-5
114-115	0.1260888923049509	2.912992775902758E-4	3.329134601031724E-5	5.409843726676551E-5	0.25231927283044564	7.074411027192413E-5
116-117	0.12609721514145347	2.912992775902758E-4	3.329134601031724E-5	5.409843726676551E-5	0.2528623379122389	7.074411027192413E-5
118-119	0.1261034572688304	2.912992775902758E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2533804344845245	7.490552852321379E-5
120-121	0.12610969939620736	2.912992775902758E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2540004858039666	7.490552852321379E-5
122-123	0.12611386081445863	2.9546069584156546E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2546413442146652	7.698623764885862E-5
124-125	0.1261221836509612	2.9546069584156546E-4	3.7452764261606895E-5	5.409843726676551E-5	0.25534046248088194	8.114765590014827E-5
126-127	0.12613050648746377	2.9546069584156546E-4	3.7452764261606895E-5	5.409843726676551E-5	0.255966755927701	8.322836502579309E-5
128-129	0.1261346679057151	2.9962211409285516E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2566034529201483	8.530907415143791E-5
130-131	0.12613882932396636	3.058642414697896E-4	3.7452764261606895E-5	5.825985551805517E-5	0.2572776026768573	9.15512015283724E-5
132-133	0.1261492328695946	3.162677870980138E-4	4.1614182512896545E-5	5.825985551805517E-5	0.2579434295970636	9.571261977966206E-5
134-135	0.1261637978334741	3.183484962236586E-4	4.57756007641862E-5	5.825985551805517E-5	0.2586134179355213	9.77933289053069E-5
136-137	0.12616587854259975	3.2042920534930343E-4	4.57756007641862E-5	5.825985551805517E-5	0.2593271011656174	9.987403803095172E-5
138-139	0.12616587854259975	3.2042920534930343E-4	4.57756007641862E-5	5.825985551805517E-5	0.25996587886719036	1.0403545628224136E-4
>>END_MODULE
