##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24118417
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	65-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.79694110936054	37.0	35.0	39.0	34.0	39.0
2	35.91139841391746	36.0	35.0	38.0	34.0	39.0
3	36.051629922477915	36.0	35.0	38.0	34.0	39.0
4	36.16496683841232	37.0	35.0	38.0	34.0	39.0
5	36.00843160643586	37.0	35.0	38.0	34.0	39.0
6	35.92226964978672	37.0	35.0	38.0	34.0	39.0
7	35.93574939018593	36.0	35.0	38.0	34.0	39.0
8	36.119591679669526	37.0	35.0	39.0	34.0	39.0
9	36.115537309102834	37.0	35.0	39.0	34.0	39.0
10-14	36.0311413970494	36.8	35.0	38.4	34.0	39.0
15-19	36.04400228257103	37.0	35.0	38.0	34.0	39.0
20-24	35.970998411711676	37.0	35.0	38.0	34.0	39.0
25-29	35.974168138812765	37.0	35.0	38.0	34.0	39.0
30-34	35.958777576488544	37.0	35.0	38.0	34.0	39.0
35-39	35.88274825831231	37.0	35.0	38.0	34.0	39.0
40-44	35.86962110324239	36.6	35.0	38.0	33.8	39.0
45-49	35.869444972279894	36.2	35.0	38.0	33.8	39.0
50-54	35.81748047560501	36.2	35.0	38.0	33.2	39.0
55-59	35.84678266405295	36.0	35.0	38.0	33.4	39.0
60-64	35.76515662698758	36.0	35.0	38.0	33.0	39.0
65-69	35.7568102614771	36.0	35.0	38.0	33.0	39.0
70-74	35.7227494993021	36.0	35.0	38.0	33.0	39.0
75-79	35.66701757025546	36.0	35.0	38.0	33.0	39.0
80-84	35.649141317250034	36.0	35.0	38.0	33.0	39.0
85-89	35.5447098830514	36.0	35.0	38.0	33.0	39.0
90-94	35.50288331938344	36.0	35.0	38.0	32.8	39.0
95-99	35.41592116755667	36.0	34.8	38.0	32.2	39.0
100-104	35.358361925995084	36.0	34.4	38.0	32.0	39.0
105-109	35.21625358571676	36.0	34.0	38.0	31.8	39.0
110-114	35.11109975564289	36.0	34.0	38.0	31.4	39.0
115-119	35.05559250321575	36.0	34.0	38.0	31.4	39.0
120-124	35.021490506709384	36.0	34.0	38.0	31.4	39.0
125-129	34.97243141048381	36.0	34.0	38.0	31.0	39.0
130-134	34.87743084519693	36.0	34.0	38.0	30.8	39.0
135-139	34.72147828078384	36.0	34.0	38.0	30.4	39.0
140-144	34.634162501053915	36.0	34.0	38.0	29.8	39.0
145-149	34.711137752814764	36.0	34.0	38.0	30.4	39.0
150	35.43288520913419	36.0	34.0	38.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	80.0
22	882.0
23	5232.0
24	18916.0
25	45270.0
26	86471.0
27	148336.0
28	230573.0
29	325754.0
30	441139.0
31	596898.0
32	828653.0
33	1212507.0
34	1932494.0
35	3560037.0
36	1.1207263E7
37	3477891.0
38	18.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.53266236337153	19.308427248770098	16.61176602096232	32.54714436689605
2	24.010995414831747	24.96445351284871	32.90786041223186	18.116690660087684
3	20.29522086793673	28.619917302201053	30.75648787397614	20.328373955886075
4	23.500746338368725	33.400131526045016	22.35559655511388	20.743525580472383
5	25.92530844789689	34.357205118395626	22.328476201402438	17.389010232305047
6	19.635986059947466	38.58175269131469	22.426123571874555	19.356137676863288
7	20.671651045754786	20.605995824684513	37.7858256617754	20.936527467785304
8	21.77736623427649	23.16155741067086	28.7444777159297	26.316598639122958
9	23.782037602219084	22.73215111920488	28.637878680014527	24.847932598561506
10-14	23.18444282640938	28.582697612368175	25.79006242408032	22.442797137142126
15-19	23.25498725724827	27.655567942124893	27.054365964399736	22.035078836227104
20-24	23.099831966583874	27.79538060064224	26.916374320918322	22.18841311185556
25-29	23.299899823441976	27.412756815673266	26.860886433798704	22.426456927086054
30-34	23.14985432087023	27.22621472213537	27.024112735093688	22.599818221900716
35-39	23.489832686780396	27.011107735636212	26.956639815954752	22.542419761628636
40-44	23.584799118449606	26.7288387956805	27.041493643633412	22.644868442236486
45-49	23.556138228774618	26.546919945425273	27.15420275844972	22.74273906735039
50-54	23.68356900105454	26.522934079934846	26.96868541747352	22.824811501537095
55-59	23.76942317565867	26.43839187289945	27.036496632428243	22.75568831901364
60-64	23.543184446972617	26.39790828726446	27.083686296658687	22.97522096910423
65-69	23.65105920431142	26.338950504369365	27.064550400528383	22.945439890790837
70-74	23.86880635751293	26.287191885920386	26.84346564334006	23.000536113226623
75-79	23.714920866634674	26.402449283737667	27.054838385202867	22.82779146442479
80-84	24.022231423509062	26.369237059745043	26.746780055444553	22.861751461301342
85-89	23.831857695542517	26.466135184086646	26.93271958733533	22.769287533035502
90-94	23.8279308744632	26.250153400928117	26.94452525718346	22.97739046742522
95-99	23.792384262177137	26.47703638483411	26.980191626746503	22.75038772624225
100-104	24.02586276383628	26.24371786787479	26.91850700948452	22.811912358804406
105-109	23.86760744180756	26.451164308212014	26.871011382922987	22.810216867057434
110-114	23.954050125941144	26.5390927394663	26.764620630091834	22.742236504500724
115-119	24.05047147724783	26.586711558362055	26.87663054863395	22.48618641575617
120-124	23.96056920633144	26.65597732564159	27.068514709501702	22.31493875852527
125-129	24.03639342514351	26.712087895828496	26.89649388515867	22.35502479386933
130-134	24.202728349926574	26.736127296454914	26.83703122244748	22.224113131171038
135-139	24.004070097697575	26.832731424136192	27.00547432852007	22.157724149646167
140-144	24.00234674954239	26.928089443946458	26.882042734520322	22.187521071990833
145-149	23.830791136914684	27.19182514275092	26.663637382176063	22.31374633815833
150	24.833228878384602	25.58596702372442	26.403604547535505	23.177199550355475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	0.5
5	0.5
6	0.5
7	1.0
8	1.0
9	3.5
10	4.5
11	6.0
12	12.0
13	15.0
14	22.5
15	40.5
16	65.0
17	118.0
18	223.5
19	394.0
20	649.0
21	1052.5
22	1613.0
23	2484.0
24	4031.5
25	6264.5
26	9504.0
27	14654.0
28	21411.0
29	30019.0
30	42468.0
31	60152.0
32	86027.0
33	116302.0
34	153574.5
35	213625.0
36	300539.0
37	399148.0
38	514741.0
39	666935.5
40	852132.0
41	1045487.0
42	1235469.0
43	1414915.0
44	1548873.5
45	1625934.0
46	1641983.0
47	1592692.5
48	1524890.0
49	1455521.0
50	1334120.0
51	1168613.0
52	997328.0
53	825395.5
54	672300.5
55	559102.0
56	460106.5
57	378860.5
58	320385.0
59	262866.0
60	200335.0
61	136351.0
62	84970.0
63	53365.0
64	33564.0
65	17486.0
66	10237.0
67	5866.5
68	3540.0
69	2364.0
70	1683.5
71	1453.5
72	1274.5
73	1063.0
74	912.5
75	557.0
76	171.0
77	79.5
78	37.5
79	22.5
80	16.5
81	10.5
82	6.5
83	5.0
84	5.0
85	3.5
86	3.0
87	2.5
88	2.0
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.2924181964347E-7
50-54	0.0812648690832404
55-59	0.0
60-64	0.0
65-69	0.0
70-74	3.3169677736761903E-6
75-79	2.4877273568237654E-6
80-84	0.0
85-89	0.0
90-94	5.804751318317072E-6
95-99	0.0830302548529445
100-104	0.0
105-109	0.0
110-114	8.293021439873691E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	3.3177073739629507E-6
135-139	0.0
140-144	0.0
145-149	8.448787136052442E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	1.0
68-69	0.0
70-71	1.0
72-73	3.0
74-75	7.0
76-77	11.0
78-79	9.0
80-81	16.0
82-83	27.0
84-85	29.0
86-87	34.0
88-89	37.0
90-91	62.0
92-93	76.0
94-95	88.0
96-97	112.0
98-99	138.0
100-101	155.0
102-103	163.0
104-105	179.0
106-107	207.0
108-109	215.0
110-111	179.0
112-113	194.0
114-115	234.0
116-117	233.0
118-119	244.0
120-121	252.0
122-123	326.0
124-125	421.0
126-127	500.0
128-129	519.0
130-131	658.0
132-133	823.0
134-135	1087.0
136-137	1566.0
138-139	4713.0
140-141	9073.0
142-143	8749.0
144-145	58819.0
146-147	778758.0
148-149	1061304.0
150-151	2.2188194E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.94854578234211
#Duplication Level	Percentage of total
1	22.04350139103257
2	9.619910813313178
3	6.187370275872272
4	4.314149703967061
5	3.30692647706336
6	2.564909283327448
7	2.1674577193709257
8	1.784517825251854
9	1.4895526819694922
>10	18.671659123033667
>50	6.3902357923699045
>100	12.973759475965016
>500	3.592642830274532
>1k	4.165675779636759
>5k	0.5245837213155459
>10k+	0.20314710623623497
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0731045491086749E-4	0.0	0.0	0.0	0.0018243320032156338	0.0
2	9.163122107060343E-4	2.073104549108675E-5	0.0	8.292418196434699E-6	0.009382871189265863	0.0
3	0.0014304421388849856	2.48772545893041E-5	0.0	8.292418196434699E-6	0.012915441340947045	0.0
4	0.0040135304070743946	2.48772545893041E-5	0.0	8.292418196434699E-6	0.018206004150272383	0.0
5	0.004158647725512002	2.902346368752145E-5	0.0	8.292418196434699E-6	0.02176759776564109	4.1462090982173495E-6
6	0.00511642202720021	2.902346368752145E-5	0.0	8.292418196434699E-6	0.028501041341146064	4.1462090982173495E-6
7	0.00677490566648715	3.3169672785738796E-5	4.1462090982173495E-6	8.292418196434699E-6	0.03745685299329554	4.1462090982173495E-6
8	0.01201571396663388	3.3169672785738796E-5	4.1462090982173495E-6	8.292418196434699E-6	0.050961056026189444	8.292418196434699E-6
9	0.012554721149402136	4.14620909821735E-5	4.1462090982173495E-6	8.292418196434699E-6	0.06046002107020539	8.292418196434699E-6
10-11	0.013066777973031978	4.560830008039085E-5	4.1462090982173495E-6	8.292418196434699E-6	0.10339608938679516	8.292418196434699E-6
12-13	0.013514568555639452	4.560830008039085E-5	4.1462090982173495E-6	8.292418196434699E-6	0.12512015195690496	8.292418196434699E-6
14-15	0.01525183016779252	4.560830008039085E-5	8.292418196434699E-6	8.292418196434699E-6	0.13565981548457345	8.292418196434699E-6
16-17	0.017262741580427936	4.560830008039085E-5	1.243862729465205E-5	8.292418196434699E-6	0.1423559431781945	8.292418196434699E-6
18-19	0.02221538834824856	5.182761372771687E-5	1.243862729465205E-5	8.292418196434699E-6	0.14925730822217725	8.292418196434699E-6
20-21	0.02689023910648862	5.3900718276825546E-5	1.243862729465205E-5	8.292418196434699E-6	0.15446494684953826	8.292418196434699E-6
22-23	0.029137484437722426	5.3900718276825546E-5	1.6584836392869398E-5	8.292418196434699E-6	0.1596124654449751	8.292418196434699E-6
24-25	0.0336713640866231	6.012003192415157E-5	1.6584836392869398E-5	8.292418196434699E-6	0.1649921717499121	8.292418196434699E-6
26-27	0.037473437829688414	7.048555466969495E-5	1.6584836392869398E-5	8.292418196434699E-6	0.16946800447143773	1.243862729465205E-5
28-29	0.04579073328071241	7.46317637679123E-5	1.6584836392869398E-5	8.292418196434699E-6	0.17303167119135554	1.243862729465205E-5
30-31	0.0655515658428163	7.877797286612965E-5	2.073104549108675E-5	8.292418196434699E-6	0.17689801117544324	1.4511731843760724E-5
32-33	0.08385085969779857	9.12166001607817E-5	2.6950359138412773E-5	8.292418196434699E-6	0.1808493484460444	1.6584836392869398E-5
34-35	0.09777797605871065	9.12166001607817E-5	2.902346368752145E-5	1.0365522745543375E-5	0.18450215866157385	1.6584836392869398E-5
36-37	0.10018899664932404	9.95090183572164E-5	3.31696727857388E-5	1.243862729465205E-5	0.18672038052912016	1.8657940941978076E-5
38-39	0.10226832051208004	1.0158212290632507E-4	3.9388986433064826E-5	1.243862729465205E-5	0.18853227390504113	2.073104549108675E-5
40-41	0.10349352530060327	1.0780143655365109E-4	4.14620909821735E-5	1.243862729465205E-5	0.19016795339428785	2.073104549108675E-5
42-43	0.10378790614657671	1.1402075020097713E-4	4.14620909821735E-5	1.243862729465205E-5	0.1917518052698069	2.073104549108675E-5
44-45	0.10456739345704157	1.160938547500858E-4	4.14620909821735E-5	1.243862729465205E-5	0.19303091077660695	2.073104549108675E-5
46-47	0.1059646659231408	1.2024006384830315E-4	4.14620909821735E-5	1.243862729465205E-5	0.19458366608388933	2.073104549108675E-5
48-49	0.10723133280264621	1.2853248204473783E-4	4.14620909821735E-5	1.243862729465205E-5	0.19592911093626086	2.073104549108675E-5
50-51	0.1082098381498255	1.2853248204473783E-4	4.14620909821735E-5	1.243862729465205E-5	0.1973657723887932	2.073104549108675E-5
52-53	0.10910127310594224	1.3060558659384652E-4	4.14620909821735E-5	1.243862729465205E-5	0.19862207374555305	2.073104549108675E-5
54-55	0.10958637957043366	1.3267869114295518E-4	4.14620909821735E-5	1.243862729465205E-5	0.19976850056121012	2.2804150040195424E-5
56-57	0.11052134972208168	1.3267869114295518E-4	4.14620909821735E-5	1.243862729465205E-5	0.20105797159075572	2.48772545893041E-5
58-59	0.11074317190883631	1.3267869114295518E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20212147422444848	2.48772545893041E-5
60-61	0.11104169896390795	1.3682490024117253E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20305229816699827	2.6950359138412773E-5
62-63	0.11128010598705546	1.430442138884986E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20402873040962846	2.902346368752145E-5
64-65	0.11131949497348852	1.492635275358246E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20512332961155783	3.3169672785738796E-5
66-67	0.11141900399184573	1.59629050281368E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20599196041763437	3.3169672785738796E-5
68-69	0.11147290471012256	1.6999457302691135E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20676730151900102	3.3169672785738796E-5
70-71	0.11159107166942175	1.6999457302691135E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20756130056130964	3.3169672785738796E-5
72-73	0.11165533791044413	1.6999457302691135E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20808994222133237	3.3169672785738796E-5
74-75	0.1116739958513861	1.7206767757602E-4	4.14620909821735E-5	1.6584836392869398E-5	0.2088424791726588	3.3169672785738796E-5
76-77	0.11172789656966292	1.7621388667423738E-4	4.560830008039085E-5	1.6584836392869398E-5	0.20979610726524878	3.3169672785738796E-5
78-79	0.11177557797429243	1.7828699122334605E-4	4.560830008039085E-5	1.6584836392869398E-5	0.210830586435254	3.3169672785738796E-5
80-81	0.11181289385617638	1.803600957724547E-4	4.97545091786082E-5	1.8657940941978076E-5	0.21167433998674123	3.9388986433064826E-5
82-83	0.11184399042441301	1.8450630487067208E-4	4.97545091786082E-5	2.48772545893041E-5	0.21243931556536236	4.14620909821735E-5
84-85	0.11187301388810053	1.8657940941978074E-4	4.97545091786082E-5	2.902346368752145E-5	0.2133348967305773	4.3535195531282174E-5
86-87	0.11190825666543538	1.886525139688894E-4	4.97545091786082E-5	2.902346368752145E-5	0.21425120894128333	4.560830008039085E-5
88-89	0.11194142633822113	1.907256185179981E-4	4.97545091786082E-5	2.902346368752145E-5	0.21522971428846263	4.768140462949952E-5
90-91	0.11198288842920329	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21588066911688275	4.97545091786082E-5
92-93	0.11201191189289081	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21647565012247694	5.182761372771687E-5
94-95	0.1120575201929712	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21719916361011588	6.219313647326024E-5
96-97	0.11206373950661853	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21788121500677263	6.633934557147759E-5
98-99	0.11208447055210961	1.969449321653241E-4	4.97545091786082E-5	2.902346368752145E-5	0.21855704708978205	7.048555466969495E-5
100-101	0.11211971332944447	1.990180367144328E-4	4.97545091786082E-5	2.902346368752145E-5	0.21910227358619763	7.46317637679123E-5
102-103	0.11214251747948467	2.0109114126354148E-4	5.182761372771687E-5	3.109656823663012E-5	0.21974908220551953	7.877797286612965E-5
104-105	0.11216324852497575	2.052373503617588E-4	5.3900718276825546E-5	3.524277733484748E-5	0.2203855253020959	8.085107741523833E-5
106-107	0.11217361404772129	2.1560287310730218E-4	5.3900718276825546E-5	3.731588188395615E-5	0.2209390442167079	8.707039106256435E-5
108-109	0.11221507613870346	2.2804150040195423E-4	5.3900718276825546E-5	3.731588188395615E-5	0.22154646384959675	9.328970470989037E-5
110-111	0.11224409960239098	2.3218770950017158E-4	5.3900718276825546E-5	3.9388986433064826E-5	0.22217876073707493	1.0365522745543374E-4
112-113	0.11225446512513654	2.404801276966063E-4	5.3900718276825546E-5	4.14620909821735E-5	0.22277166863811998	1.0365522745543374E-4
114-115	0.11226483064788206	2.404801276966063E-4	5.3900718276825546E-5	4.14620909821735E-5	0.22326092131170963	1.0780143655365109E-4
116-117	0.11227726927517673	2.404801276966063E-4	5.3900718276825546E-5	4.14620909821735E-5	0.2238102940172234	1.1402075020097713E-4
118-119	0.11228763479792227	2.4462633679482365E-4	5.3900718276825546E-5	4.14620909821735E-5	0.2243264970499515	1.1816695929919448E-4
120-121	0.11230007342521692	2.4462633679482365E-4	6.012003192415157E-5	4.14620909821735E-5	0.22486135802362153	1.2024006384830315E-4
122-123	0.11231665826160979	2.48772545893041E-4	6.219313647326024E-5	4.14620909821735E-5	0.22553097079298362	1.2231316839741183E-4
124-125	0.11233531620255177	2.5291875499125835E-4	6.219313647326024E-5	4.560830008039085E-5	0.22620265666689485	1.2853248204473783E-4
126-127	0.11234775482984642	2.5706496408947567E-4	6.219313647326024E-5	4.560830008039085E-5	0.2268183687179801	1.409711093393899E-4
128-129	0.11235190103894463	2.653573822859104E-4	6.219313647326024E-5	4.560830008039085E-5	0.22747554286004756	1.59629050281368E-4
130-131	0.11236019345714107	2.7572290503145375E-4	6.219313647326024E-5	4.560830008039085E-5	0.22816795977944987	1.65848363928694E-4
132-133	0.1123892169208286	2.819422186787798E-4	6.219313647326024E-5	4.560830008039085E-5	0.22871111317131634	1.741407821251287E-4
134-135	0.112416167279967	2.8608842777699714E-4	6.633934557147759E-5	4.560830008039085E-5	0.2292729245041248	1.7828699122334605E-4
136-137	0.11244519074365453	2.881615323261058E-4	6.633934557147759E-5	4.560830008039085E-5	0.22988034413701364	1.8450630487067208E-4
138-139	0.11244933695275275	2.902346368752145E-4	6.633934557147759E-5	4.560830008039085E-5	0.23041727821523278	1.8657940941978074E-4
>>END_MODULE
