##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24075394
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	71-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.50897119274559	37.0	35.0	39.0	33.0	39.0
2	35.70733077099382	36.0	35.0	39.0	33.0	39.0
3	35.899094320117875	37.0	35.0	39.0	33.0	39.0
4	35.973079734437576	37.0	35.0	39.0	33.0	39.0
5	35.79715866747601	36.0	35.0	38.0	33.0	39.0
6	35.65718480038167	36.0	35.0	38.0	33.0	39.0
7	35.49237615799766	35.0	35.0	37.0	33.0	39.0
8	35.74073566563438	36.0	35.0	38.0	33.0	39.0
9	35.732493225240674	36.0	35.0	38.0	33.0	39.0
10-14	35.754093071124814	36.2	35.0	38.2	33.0	39.0
15-19	35.75368225334132	36.0	35.0	38.0	33.0	39.0
20-24	35.74944982416488	36.0	35.0	38.0	33.0	39.0
25-29	35.74442117125892	36.0	35.0	38.0	33.0	39.0
30-34	35.73153589095987	36.0	35.0	38.0	33.0	39.0
35-39	35.72620346732435	36.0	35.0	38.0	33.0	39.0
40-44	35.71521101586126	36.0	35.0	38.0	33.0	39.0
45-49	35.70447603058957	36.0	35.0	38.0	33.0	39.0
50-54	35.68986108389337	36.0	35.0	38.0	33.0	39.0
55-59	35.68310926084948	36.0	35.0	38.0	33.0	39.0
60-64	35.67222185439623	36.0	35.0	38.0	33.0	39.0
65-69	35.655017018620754	36.0	35.0	38.0	33.0	39.0
70-74	35.652934609990375	36.0	35.0	38.0	33.0	39.0
75-79	35.64339601340725	36.0	35.0	38.0	33.0	39.0
80-84	35.62090717172691	36.0	35.0	38.0	33.0	39.0
85-89	35.60571334788093	36.0	35.0	38.0	33.0	39.0
90-94	35.5984264061952	36.0	35.0	38.0	33.0	39.0
95-99	35.57171892393169	36.0	35.0	38.0	33.0	39.0
100-104	35.55123594121533	36.0	35.0	38.0	33.0	39.0
105-109	35.525970611811886	36.0	35.0	38.0	33.0	39.0
110-114	35.50869755752974	36.0	35.0	38.0	33.0	39.0
115-119	35.47488397505765	36.0	34.2	38.0	33.0	39.0
120-124	35.45476665357447	36.0	34.0	38.0	32.6	39.0
125-129	35.42803590651771	36.0	34.0	38.0	32.0	39.0
130-134	35.39331000564273	36.0	34.0	38.0	32.0	39.0
135-139	35.359769610609185	36.0	34.0	38.0	32.0	39.0
140-144	35.32447465481682	36.0	34.0	38.0	32.0	39.0
145-149	35.31657333637047	36.0	34.0	38.0	32.0	39.0
150	35.76903749164315	36.0	34.0	38.0	33.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	87.0
23	1299.0
24	9934.0
25	41957.0
26	108228.0
27	196265.0
28	289279.0
29	375676.0
30	469517.0
31	594304.0
32	772957.0
33	1066727.0
34	1600433.0
35	2909692.0
36	1.0750274E7
37	4888711.0
38	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.58478419917032	10.800359072005218	15.413488144783841	38.201368584040615
2	23.510350801065137	11.879154736603775	35.246824526114494	29.363669936216592
3	21.763181231944408	16.64953821295579	27.895945785972557	33.691334769127245
4	26.780204475886666	23.955347384998255	21.816935028270297	27.447513110844785
5	27.121960284558615	29.964285834444325	23.753818277230863	19.159935603766197
6	20.08873378348882	36.4763554144617	24.787562068062464	18.647348733987016
7	16.476788028001053	25.347539714641243	39.57004953359403	18.605622723763673
8	18.871260511824794	24.538342831207657	30.954522143299297	25.635874513668256
9	19.783154517276397	23.0117665373839	32.32037094141603	24.884708003923674
10-14	20.628451799070653	29.14882565385198	26.628533990919074	23.594188556158294
15-19	20.97546663654351	27.846970114232267	27.27555664299167	23.90200660623255
20-24	21.002087603588603	27.938002429222625	27.100166434920702	23.95974353226807
25-29	20.95409988872687	28.134760422665277	26.82137575600448	24.089763932603372
30-34	20.928225755730427	28.03076022544278	26.934768338446712	24.106245680380077
35-39	21.14050751989005	27.764292708991338	26.89051275345839	24.20468701766022
40-44	21.245043792072575	27.811502896612726	26.803443287789403	24.140010023525296
45-49	21.470212710369488	27.69787764805941	26.737203357513145	24.09470628405796
50-54	21.48093657469626	27.837742138245243	26.661507749739716	24.019813537318786
55-59	21.400419785786667	27.77535298087833	26.786836388862646	24.03739084447236
60-64	21.623408653904225	27.657658736856643	26.5395401886389	24.17939242060023
65-69	21.53309389986286	27.702169144095112	26.706853604000802	24.05788335204123
70-74	21.813869680380353	27.51064499830436	26.640756678669312	24.034728642645966
75-79	21.66219848407021	27.476615217915583	26.615200054980747	24.245986243033464
80-84	21.92898550590999	27.390374212405984	26.60940170746335	24.07123857422068
85-89	21.793664462260143	27.402828574257075	26.67953694030129	24.123970023181496
90-94	21.8035876638323	27.184865761315624	26.641584026519126	24.36996254833295
95-99	22.007946667373783	27.220030644647792	26.760286992765568	24.011735695212856
100-104	21.998517206401974	27.0439506262928	26.806773598405243	24.15075856889998
105-109	22.087288644870597	26.961351019327967	26.757463909828346	24.193896425973087
110-114	22.010622744241935	26.985051311008856	26.75929024905078	24.245035695698434
115-119	22.141494483383443	27.16452412809818	26.639794933311183	24.054186455207198
120-124	22.057806500995174	27.02065389474252	26.71581135824187	24.205728246020435
125-129	22.376478753951233	26.974486006113285	26.639362865938214	24.009672373997265
130-134	22.151617706150134	27.133503364717438	26.539215156777658	24.17566377235477
135-139	22.2929962923107	26.96157562293669	26.559623417292364	24.185804667460243
140-144	22.26671727888529	26.804469038185218	26.79178385160042	24.13702983132907
145-149	21.850222332597813	26.8710606040519	27.064682788351984	24.214034274998305
150	22.438795875024116	25.244525260401012	27.21852850258779	25.098150361987077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.5
10	3.5
11	9.0
12	11.5
13	15.0
14	29.5
15	75.0
16	136.5
17	275.0
18	652.0
19	1178.5
20	1953.0
21	3141.5
22	5121.0
23	8228.5
24	12937.5
25	19405.0
26	27382.5
27	38548.5
28	57215.0
29	83883.5
30	114655.0
31	145373.0
32	178023.5
33	215149.5
34	252032.5
35	299108.5
36	369461.0
37	448268.5
38	529722.0
39	640216.0
40	786733.5
41	949747.5
42	1111212.5
43	1264345.0
44	1400208.5
45	1494739.5
46	1546944.5
47	1550721.5
48	1493315.0
49	1424629.5
50	1325352.5
51	1175373.5
52	1001383.5
53	845232.5
54	699236.5
55	565159.5
56	474920.5
57	392065.5
58	319862.5
59	257344.0
60	197683.5
61	137520.0
62	80221.5
63	45840.0
64	30210.0
65	19752.0
66	13726.0
67	7146.5
68	3406.0
69	2159.0
70	1626.0
71	1577.0
72	1585.5
73	1213.0
74	628.0
75	186.5
76	59.5
77	40.0
78	24.0
79	17.5
80	17.5
81	12.0
82	6.5
83	3.5
84	1.5
85	2.5
86	2.5
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.568980262586772E-5
3	0.0018442065787168424
4	0.0019812759865944457
5	3.0321414469894033E-4
6	3.738256578480086E-5
7	6.645789472853486E-5
8	2.9075328943734005E-5
9	8.307236841066858E-6
10-14	5.731993420336133E-5
15-19	3.239822368016075E-5
20-24	1.5700677629616362E-4
25-29	1.6863690787365722E-4
30-34	2.8992256575323334E-4
35-39	1.7445197366240404E-4
40-44	1.3291578945706973E-4
45-49	3.115213815400072E-4
50-54	2.3426407891808542E-4
55-59	2.7912315785984644E-4
60-64	6.114126315025208E-4
65-69	7.252217762251368E-4
70-74	3.9874737830867597E-4
75-79	2.259568702331771E-4
80-84	2.6832389708245026E-4
85-89	1.2211659054067942E-4
90-94	2.3177282050143743E-4
95-99	9.138026112923107E-5
100-104	1.0384163283707029E-4
105-109	6.812044049434871E-5
110-114	1.3458018831755906E-4
115-119	9.885901083434946E-5
120-124	1.5701260930881404E-4
125-129	1.8525980140863743E-4
130-134	1.354161320291004E-4
135-139	1.7280625840462554E-4
140-144	1.404384881831649E-4
145-149	1.0724172369808273E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
70-71	1.0
72-73	0.0
74-75	1.0
76-77	3.0
78-79	2.0
80-81	5.0
82-83	11.0
84-85	9.0
86-87	19.0
88-89	20.0
90-91	22.0
92-93	29.0
94-95	31.0
96-97	38.0
98-99	36.0
100-101	46.0
102-103	41.0
104-105	47.0
106-107	51.0
108-109	45.0
110-111	66.0
112-113	83.0
114-115	68.0
116-117	62.0
118-119	75.0
120-121	97.0
122-123	49.0
124-125	88.0
126-127	98.0
128-129	124.0
130-131	177.0
132-133	218.0
134-135	245.0
136-137	462.0
138-139	1475.0
140-141	4107.0
142-143	4508.0
144-145	54233.0
146-147	716042.0
148-149	648067.0
150-151	2.2644593E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.43675913097829
#Duplication Level	Percentage of total
1	32.055496683926854
2	9.783610239017488
3	5.599668234509204
4	3.9413788721484946
5	2.9628779825062552
6	2.320307795991038
7	1.85941022377611
8	1.6205384474205313
9	1.4260151415222158
>10	16.622200877185005
>50	5.202333751412773
>100	10.493378597804735
>500	3.218853447623272
>1k	2.8151684060443927
>5k	0.07876129911159471
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.153618420533429E-6	2.0768092102667146E-5	0.0	0.0	2.3675624997040548E-4	4.153618420533429E-6
2	4.153618420533429E-6	9.553322367226888E-5	0.0	1.2460855261600288E-5	0.0021349598681541827	1.2460855261600288E-5
3	1.2460855261600288E-5	1.0384046051333573E-4	0.0	1.2460855261600288E-5	0.003891940460039823	3.322894736426743E-5
4	4.984342104640115E-5	1.2460855261600288E-4	0.0	2.0768092102667146E-5	0.00563230657824333	3.738256578480086E-5
5	4.984342104640115E-5	1.2460855261600288E-4	0.0	2.4921710523200576E-5	0.0070071542754398955	4.153618420533429E-5
6	8.722598683120202E-5	1.2460855261600288E-4	0.0	2.4921710523200576E-5	0.008593836512083666	4.153618420533429E-5
7	1.1630131577493602E-4	1.4537664471867004E-4	0.0	2.4921710523200576E-5	0.01027189835397917	4.153618420533429E-5
8	2.0352730260613804E-4	1.4953026313920345E-4	0.0	2.4921710523200576E-5	0.013337268748332841	6.645789472853486E-5
9	2.450634868114723E-4	1.4953026313920345E-4	0.0	2.4921710523200576E-5	0.01629049144533211	6.645789472853486E-5
10-11	3.0321414469894033E-4	1.5783749998027032E-4	0.0	2.4921710523200576E-5	0.02822591397673492	9.761003288253558E-5
12-13	3.6344161179667505E-4	1.6199111840080373E-4	0.0	2.4921710523200576E-5	0.03731610789007233	1.2045493419546944E-4
14-15	4.0497779600200937E-4	1.6822154603160388E-4	0.0	2.9075328943734002E-5	0.0446119386457393	1.3499259866733645E-4
16-17	4.3197631573547665E-4	1.7860559208293745E-4	0.0	3.322894736426743E-5	0.04965650821747715	1.3706940787760316E-4
18-19	4.735124999408109E-4	1.9314325655480448E-4	0.0	3.322894736426743E-5	0.05338230394069563	1.3706940787760316E-4
20-21	6.936542762290827E-4	2.2429539470880517E-4	0.0	3.322894736426743E-5	0.056526593085039434	1.3706940787760316E-4
22-23	9.449481906713551E-4	2.284490131293386E-4	0.0	3.5305756574534143E-5	0.05960442433465471	1.4329983550840332E-4
24-25	0.0010342509867128239	2.3467944076013876E-4	0.0	3.738256578480086E-5	0.06276948157110118	1.4953026313920345E-4
26-27	0.0010944784538105587	2.3675624997040548E-4	0.0	4.153618420533429E-5	0.06564586232732059	1.5160707234947017E-4
28-29	0.0011443218748569597	2.3883305918067217E-4	0.0	4.568980262586772E-5	0.06894798897164466	1.6406792761107045E-4
30-31	0.001237778289318962	2.450634868114723E-4	0.0	5.60738486772013E-5	0.07243702844489273	1.7652878287267073E-4
32-33	0.001273084045893496	2.450634868114723E-4	0.0	6.645789472853488E-5	0.07574330870763735	2.0352730260613804E-4
34-35	0.0013312347037809642	2.4921710523200576E-4	0.0	1.0176365130306901E-4	0.07923650179930597	2.1806496707800504E-4
36-37	0.0013665404603554982	2.512939144422725E-4	0.0	1.1214769735440259E-4	0.08239948222654217	2.242953947088052E-4
38-39	0.0013727708879862984	2.533707236525392E-4	0.0	1.1630131577493602E-4	0.0853111687393361	2.3052582233960533E-4
40-41	0.0014059998353505659	2.533707236525392E-4	0.0	1.1630131577493602E-4	0.0882249320613403	2.3260263154987204E-4
42-43	0.0014163838814018994	2.554475328628059E-4	0.0	1.1630131577493602E-4	0.09086040294916878	2.3260263154987204E-4
44-45	0.001426767927453233	2.61677960493606E-4	0.0	1.1837812498520273E-4	0.0937347068961779	2.3675624997040548E-4
46-47	0.0014454592103456333	2.61677960493606E-4	2.0768092102667145E-6	1.2045493419546944E-4	0.09812923518510226	2.409098683909389E-4
48-49	0.0014745345392893673	2.679083881244062E-4	4.153618420533429E-6	1.2876217103653632E-4	0.10116968386893274	2.4714029602173904E-4
50-51	0.0014849185853407008	2.8659967101680664E-4	4.153618420533429E-6	1.2876217103653632E-4	0.10425997597380961	2.533707236525392E-4
52-53	0.0015264547695460354	2.9490690785787346E-4	4.153618420533429E-6	1.2876217103653632E-4	0.10699513370373087	2.533707236525392E-4
54-55	0.0015534532892795025	2.9490690785787346E-4	4.153618420533429E-6	1.3083898024680304E-4	0.10918201380214172	2.533707236525392E-4
56-57	0.0016282184208491043	2.990605262784069E-4	4.153618420533429E-6	1.3291578945706973E-4	0.11150596330843018	2.554475328628059E-4
58-59	0.0017009067432084393	3.0321414469894033E-4	4.153618420533429E-6	1.3291578945706973E-4	0.11377383896604143	2.5752434207307264E-4
60-61	0.0017839791116191078	3.115213815400072E-4	4.153618420533429E-6	1.3706940787760316E-4	0.11602925376839107	2.5752434207307264E-4
62-63	0.0018213616774039086	3.1567499996054064E-4	4.153618420533429E-6	1.3706940787760316E-4	0.11867510870227088	2.61677960493606E-4
64-65	0.001840052960296309	3.198286183810741E-4	4.153618420533429E-6	1.4329983550840332E-4	0.12196685130054362	2.61677960493606E-4
66-67	0.0018525138155579095	3.3228947364267433E-4	4.153618420533429E-6	1.4537664471867004E-4	0.12512983172777983	2.61677960493606E-4
68-69	0.0018670514800297766	3.426735196940079E-4	4.153618420533429E-6	1.4537664471867004E-4	0.12737278567486787	2.6583157891413946E-4
70-71	0.0019044340458145774	3.6551842100694177E-4	4.153618420533429E-6	1.4537664471867004E-4	0.12962197004958673	2.699851973346729E-4
72-73	0.0019210485194967111	3.7382565784800864E-4	4.153618420533429E-6	1.4537664471867004E-4	0.13156378666118612	2.7621562496547305E-4
74-75	0.0019522006576507117	3.7382565784800864E-4	4.153618420533429E-6	1.5783749998027032E-4	0.13363851906224256	2.845228618065399E-4
76-77	0.0019812759865944457	3.7382565784800864E-4	4.153618420533429E-6	1.5783749998027032E-4	0.1352584302462506	2.907532894373401E-4
78-79	0.0020041208879073794	3.7590246705827536E-4	4.153618420533429E-6	1.6199111840080373E-4	0.13687626462104835	2.9490690785787346E-4
80-81	0.0020269657892203136	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.13840064258138413	2.9490690785787346E-4
82-83	0.0020643483550051144	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.14017631445616216	2.9490690785787346E-4
84-85	0.0021308062497336494	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.1419769080414634	2.9490690785787346E-4
86-87	0.0021661120063081836	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.14367573797546157	2.9490690785787346E-4
88-89	0.0021868800984108506	3.821328946890755E-4	4.153618420533429E-6	1.723751644521373E-4	0.14540572004761376	3.0321414469894033E-4
90-91	0.002199340953672451	3.821328946890755E-4	4.153618420533429E-6	1.7860559208293745E-4	0.1477483608367946	3.0736776311947377E-4
92-93	0.002228416282616185	3.821328946890755E-4	4.153618420533429E-6	1.7860559208293745E-4	0.14990408879705147	3.0736776311947377E-4
94-95	0.002259568420770185	3.821328946890755E-4	4.153618420533429E-6	1.8068240129320417E-4	0.15179606198760442	3.094445723297405E-4
96-97	0.0022948741773447193	3.821328946890755E-4	4.153618420533429E-6	1.8275921050347089E-4	0.15336197613214553	3.115213815400072E-4
98-99	0.002373792927334855	3.842097038993422E-4	4.153618420533429E-6	1.9106644734453776E-4	0.15485727876353755	3.115213815400072E-4
100-101	0.0024838638154789905	3.862865131096089E-4	4.153618420533429E-6	1.9522006576507117E-4	0.15642526971728893	3.115213815400072E-4
102-103	0.002517092762843258	3.9044013153014234E-4	4.153618420533429E-6	1.9522006576507117E-4	0.15771704504607484	3.115213815400072E-4
104-105	0.0025337072365253918	3.9251694074040906E-4	4.153618420533429E-6	2.0560411181640473E-4	0.15898182185512727	3.198286183810741E-4
106-107	0.0025731666115204593	3.987473683712092E-4	4.153618420533429E-6	2.1598815786773832E-4	0.16032967103259038	3.198286183810741E-4
108-109	0.0025939347036231268	3.987473683712092E-4	4.153618420533429E-6	2.3883305918067217E-4	0.16162975359821735	3.198286183810741E-4
110-111	0.002625086841777127	4.0497779600200937E-4	4.153618420533429E-6	2.450634868114723E-4	0.16275953780860242	3.198286183810741E-4
112-113	0.0027040055917672626	4.091314144225428E-4	4.153618420533429E-6	2.4714029602173904E-4	0.1639723943873982	3.198286183810741E-4
114-115	0.0027393113483417967	4.153618420533429E-4	4.153618420533429E-6	2.512939144422725E-4	0.1655549230056214	3.198286183810741E-4
116-117	0.002766309868075264	4.2159226968414306E-4	4.153618420533429E-6	2.6583157891413946E-4	0.1667490883015248	3.198286183810741E-4
118-119	0.0027891547693881975	4.3197631573547665E-4	4.153618420533429E-6	2.679083881244062E-4	0.16767949882772426	3.198286183810741E-4
120-121	0.0028784575654296667	4.3197631573547665E-4	4.153618420533429E-6	2.7829243417573977E-4	0.16876774685390403	3.198286183810741E-4
122-123	0.00303421825619967	4.3197631573547665E-4	4.153618420533429E-6	2.9490690785787346E-4	0.16979992103140656	3.2190542759134074E-4
124-125	0.003135981907502739	4.3405312494574337E-4	4.153618420533429E-6	2.990605262784069E-4	0.17093178205100196	3.2398223680160746E-4
126-127	0.0031858253285491404	4.402835525765435E-4	4.153618420533429E-6	3.011373354886736E-4	0.1722256341889981	3.2398223680160746E-4
128-129	0.0032502064140674082	4.402835525765435E-4	4.153618420533429E-6	3.0321414469894033E-4	0.17324119389281853	3.281358552221409E-4
130-131	0.00331874111800621	4.402835525765435E-4	4.153618420533429E-6	3.094445723297405E-4	0.1739992292545659	3.302126644324076E-4
132-133	0.0033623541114218107	4.465139802073436E-4	4.153618420533429E-6	3.2190542759134074E-4	0.1748091848465699	3.3228947364267433E-4
134-135	0.0034184279600990124	4.4859078941761034E-4	4.153618420533429E-6	3.2398223680160746E-4	0.1756710606688306	3.3436628285294105E-4
136-137	0.003536806085084215	4.6105164467921065E-4	4.153618420533429E-6	3.302126644324076E-4	0.17665339142528674	3.489039473248081E-4
138-139	0.0035430365127150153	4.755893091510777E-4	4.153618420533429E-6	3.3436628285294105E-4	0.17739688912256224	3.489039473248081E-4
>>END_MODULE
