##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24075394
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.797582834989115	37.0	35.0	39.0	34.0	39.0
2	35.933263065185976	36.0	35.0	38.0	34.0	39.0
3	36.06095493182791	36.0	35.0	38.0	34.0	39.0
4	36.11562672660726	37.0	35.0	38.0	34.0	39.0
5	35.95591615240025	37.0	35.0	38.0	34.0	39.0
6	35.87792835290671	36.0	35.0	38.0	34.0	39.0
7	35.97411224090455	36.0	35.0	38.0	34.0	39.0
8	36.09702370810629	37.0	35.0	39.0	34.0	39.0
9	36.10832711605883	37.0	35.0	39.0	34.0	39.0
10-14	36.02507109956331	36.8	35.0	38.4	34.0	39.0
15-19	36.02142328387232	37.0	35.0	38.0	34.0	39.0
20-24	35.94711575644411	36.8	35.0	38.0	34.0	39.0
25-29	35.94772039867759	36.8	35.0	38.0	34.0	39.0
30-34	35.92937610906804	36.8	35.0	38.0	34.0	39.0
35-39	35.849055645776765	36.2	35.0	38.0	33.6	39.0
40-44	35.84892140913664	36.0	35.0	38.0	33.6	39.0
45-49	35.867731045232325	36.2	35.0	38.0	33.8	39.0
50-54	35.81649307172294	36.0	35.0	38.0	33.0	39.0
55-59	35.83156670250132	36.0	35.0	38.0	33.4	39.0
60-64	35.73108454303819	36.0	35.0	38.0	33.0	39.0
65-69	35.7263912221075	36.0	35.0	38.0	33.0	39.0
70-74	35.70121703162386	36.0	35.0	38.0	33.0	39.0
75-79	35.65440036812242	36.0	35.0	38.0	33.0	39.0
80-84	35.633073347672706	36.0	35.0	38.0	33.0	39.0
85-89	35.525443956580574	36.0	35.0	38.0	32.8	39.0
90-94	35.46510178404018	36.0	35.0	38.0	32.4	39.0
95-99	35.37821961794568	36.0	34.6	38.0	32.2	39.0
100-104	35.32888760178314	36.0	34.0	38.0	32.0	39.0
105-109	35.21179830492684	36.0	34.0	38.0	31.8	39.0
110-114	35.04860910509774	36.0	34.0	38.0	31.4	39.0
115-119	35.03633946130204	36.0	34.0	38.0	31.4	39.0
120-124	35.001038148768274	36.0	34.0	38.0	31.2	39.0
125-129	34.921428079506995	36.0	34.0	38.0	30.8	39.0
130-134	34.845039432705235	36.0	34.0	38.0	30.8	39.0
135-139	34.65068241236855	35.8	34.0	38.0	30.0	39.0
140-144	34.61364170245694	36.0	34.0	38.0	29.8	39.0
145-149	34.63979353704206	35.8	34.0	38.0	30.0	39.0
150	35.40736079928539	36.0	34.0	38.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	74.0
22	819.0
23	5261.0
24	19366.0
25	46381.0
26	87522.0
27	150081.0
28	231758.0
29	328690.0
30	447977.0
31	609305.0
32	847476.0
33	1244261.0
34	1976684.0
35	3612149.0
36	1.1101162E7
37	3366412.0
38	14.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.216253906374284	19.22167504299203	16.11224721805176	31.44982383258193
2	24.81794898143723	25.32073618400596	32.1291938150628	17.73212101949401
3	20.21861076161872	28.9506675965788	30.596447584469338	20.234274057333145
4	23.512911149034572	33.59055307672224	22.146769436047443	20.74976633819575
5	25.906599908603777	34.69394934928168	21.90995088180073	17.489499860313813
6	19.455415765989127	38.975029858286014	22.03376609329841	19.53578828242645
7	20.813017639503638	20.693268820439656	37.37987008644594	21.11384345361077
8	21.7536585278729	23.320640152348076	28.400930842502515	26.524770477276512
9	23.904086471025145	22.93644706292242	28.17853365141189	24.980932814640543
10-14	23.355356095106895	28.67860687970465	25.474193277999934	22.49184374718852
15-19	23.458757933515024	27.594209257800724	26.85928379822154	22.087749010462716
20-24	23.303923499652797	27.750137754754917	26.73788266974987	22.208056075842414
25-29	23.46244468522509	27.354122636580737	26.717549046133993	22.465883632060184
30-34	23.27648801926149	27.12716061884595	26.93214740327822	22.664203958614344
35-39	23.58918321336714	26.92442665735813	26.87096875756218	22.615421371712547
40-44	23.721726007890048	26.65916329344392	26.94498291492135	22.674127783744684
45-49	23.641706549018473	26.481493096229286	27.084077627140807	22.792722727611437
50-54	23.778280845580348	26.481768896492408	26.868399329207236	22.87155092872
55-59	23.888629195434973	26.42182802906569	26.926072320976346	22.763470454522988
60-64	23.633811940119738	26.42215433123514	26.965853858072368	22.97817987057275
65-69	23.76147953299733	26.371660150870902	26.95712934494598	22.90973097118579
70-74	23.95429074514981	26.344794374437264	26.706106946484393	22.994807933928538
75-79	23.808305730018784	26.4373868636268	26.941446415011633	22.812860991342788
80-84	24.11254677951211	26.41526432490252	26.64643450924794	22.825754386337426
85-89	23.907801582464376	26.50371215941052	26.842810481558594	22.74567577656651
90-94	23.905113647262283	26.31658439740982	26.84029655262471	22.93800540270319
95-99	23.869276451672032	26.52820960809375	26.88866948370417	22.71384445653005
100-104	24.14362690542416	26.295286011561142	26.830981224079313	22.730105858935385
105-109	23.92591962746306	26.49563478446456	26.817209836858076	22.761235751214304
110-114	24.015234304318213	26.61098373302765	26.67095491137083	22.702827051283307
115-119	24.106262202057767	26.635308366861366	26.81937026448498	22.439059166595882
120-124	24.02932926709102	26.71275491517165	26.988339917447963	22.26957590028937
125-129	24.06501594333391	26.79222807037266	26.812383180973143	22.330372805320284
130-134	24.237545537569726	26.786875029461132	26.777690095010392	22.19788933795875
135-139	24.039617918136745	26.877386282097852	26.94979334556558	22.133202454199814
140-144	24.07055399988334	26.96592697435841	26.840471513570602	22.123047512187647
145-149	23.84450642519567	27.06494242622713	26.789775451315755	22.300775697261443
150	24.8058514806434	25.52960826914618	26.574509730014483	23.09003052019594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	0.5
7	1.5
8	2.5
9	5.0
10	6.0
11	6.5
12	12.5
13	16.0
14	25.5
15	40.0
16	65.5
17	110.5
18	218.5
19	382.0
20	596.0
21	950.0
22	1459.5
23	2257.0
24	3658.5
25	5607.0
26	8517.5
27	13263.0
28	19600.0
29	27934.5
30	39752.0
31	55777.5
32	80211.0
33	110016.0
34	145512.0
35	202858.0
36	285980.5
37	382949.0
38	499549.5
39	650694.0
40	834086.0
41	1024874.5
42	1215072.5
43	1395229.0
44	1530269.5
45	1610894.5
46	1630414.5
47	1590631.5
48	1528698.0
49	1462058.5
50	1345630.0
51	1183790.0
52	1012986.0
53	844316.0
54	696579.5
55	578690.0
56	478523.0
57	396814.5
58	331697.0
59	271693.0
60	207545.0
61	141479.5
62	88494.0
63	54573.5
64	34030.5
65	17970.5
66	10692.0
67	6142.5
68	3731.5
69	2586.0
70	1805.5
71	1445.5
72	1241.5
73	1061.0
74	833.5
75	494.5
76	163.5
77	75.0
78	39.0
79	20.0
80	13.0
81	9.0
82	7.5
83	4.5
84	1.0
85	3.0
86	2.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.153618420533429E-6
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	8.307283285180304E-7
90-94	4.984395808943889E-6
95-99	0.10237457032055428
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.308066771434158E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	8.310137937818994E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	2.0
64-65	0.0
66-67	1.0
68-69	1.0
70-71	2.0
72-73	7.0
74-75	7.0
76-77	8.0
78-79	11.0
80-81	16.0
82-83	25.0
84-85	34.0
86-87	36.0
88-89	49.0
90-91	60.0
92-93	66.0
94-95	78.0
96-97	124.0
98-99	148.0
100-101	156.0
102-103	197.0
104-105	184.0
106-107	224.0
108-109	186.0
110-111	202.0
112-113	214.0
114-115	246.0
116-117	229.0
118-119	274.0
120-121	306.0
122-123	370.0
124-125	376.0
126-127	452.0
128-129	525.0
130-131	676.0
132-133	869.0
134-135	1151.0
136-137	1557.0
138-139	4508.0
140-141	8961.0
142-143	9245.0
144-145	59244.0
146-147	779478.0
148-149	1025477.0
150-151	2.2179412E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.964362639896656
#Duplication Level	Percentage of total
1	21.87932677859035
2	9.933301722200062
3	6.204976259664434
4	4.369748798047005
5	3.30662538276227
6	2.599066471890691
7	2.12917014408633
8	1.849170365922092
9	1.5616195711467198
>10	18.30513671569501
>50	6.2906220663859145
>100	12.984382456779345
>500	3.740753105009045
>1k	4.147725509383108
>5k	0.5107269717921404
>10k+	0.18764768064528659
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.9490690785787346E-4	4.153618420533429E-6	0.0	0.0	0.0016988299339981726	0.0
2	0.0014371519735045665	1.2460855261600288E-5	0.0	0.0	0.009054888156762876	0.0
3	0.002097577302369382	1.2460855261600288E-5	0.0	0.0	0.012448394406338688	0.0
4	0.005482776315104127	1.2460855261600288E-5	0.0	0.0	0.017495040787286805	0.0
5	0.005653074670345998	1.2460855261600288E-5	0.0	0.0	0.02120837565524369	0.0
6	0.00690331381492656	1.2460855261600288E-5	0.0	0.0	0.028198915457001452	0.0
7	0.008959354933090607	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.0391520072319481	0.0
8	0.014965487169181946	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.05351106611173217	0.0
9	0.01549299670858969	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.06340913880786334	0.0
10-11	0.016051658386151437	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.10553098321049284	0.0
12-13	0.016604089636082384	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.12648598814208398	0.0
14-15	0.018955037662104303	2.4921710523200576E-5	4.153618420533429E-6	0.0	0.1360995379764086	0.0
16-17	0.021615430260455967	2.4921710523200576E-5	8.307236841066858E-6	0.0	0.14264564060716928	0.0
18-19	0.027513568417613436	2.699851973346729E-5	8.307236841066858E-6	0.0	0.14911905491557065	0.0
20-21	0.03287588979852209	2.9075328943734005E-5	8.307236841066858E-6	0.0	0.15375033945446542	0.0
22-23	0.03514376545613335	3.322894736426743E-5	8.307236841066858E-6	0.0	0.15861838024333058	0.0
24-25	0.03976258913976652	3.5305756574534143E-5	8.307236841066858E-6	0.0	0.1629236057362135	0.0
26-27	0.04353822828403141	3.738256578480086E-5	8.307236841066858E-6	0.0	0.16632749603184066	0.0
28-29	0.052991863809165485	3.738256578480086E-5	8.307236841066858E-6	0.0	0.16923087530779352	6.230427630800143E-6
30-31	0.07369142120789383	4.153618420533429E-5	1.4537664471867001E-5	0.0	0.17241254701792214	8.307236841066858E-6
32-33	0.09182611923194278	5.399703946693458E-5	1.6614473682133716E-5	0.0	0.17506670918864298	8.307236841066858E-6
34-35	0.10631394028276339	5.399703946693458E-5	1.6614473682133716E-5	0.0	0.1778787088593441	8.307236841066858E-6
36-37	0.10877288238771918	5.815065788746801E-5	1.6614473682133716E-5	0.0	0.1797374531025328	8.307236841066858E-6
38-39	0.11095768567691977	6.022746709773472E-5	1.6614473682133716E-5	0.0	0.18125560063523777	8.307236841066858E-6
40-41	0.11232007251885473	6.645789472853486E-5	1.6614473682133716E-5	0.0	0.1827841322139941	8.307236841066858E-6
42-43	0.11258175047934833	6.853470393880158E-5	1.6614473682133716E-5	0.0	0.18435212316774546	8.307236841066858E-6
44-45	0.11328994242004928	7.2688322359335E-5	1.6614473682133716E-5	0.0	0.18563559125969029	8.307236841066858E-6
46-47	0.11463363797909185	7.476513156960172E-5	1.6614473682133716E-5	0.0	0.18691075211479405	1.0384046051333573E-5
48-49	0.11565127449212254	7.476513156960172E-5	1.6614473682133716E-5	0.0	0.18806753484491262	2.0768092102667146E-5
50-51	0.11643007794597256	8.7225986831202E-5	1.6614473682133716E-5	0.0	0.1893094667526521	2.0768092102667146E-5
52-53	0.11723172630113551	9.76100328825356E-5	1.6614473682133716E-5	0.0	0.19038110030514974	2.0768092102667146E-5
54-55	0.11770108518265579	1.0384046051333573E-4	1.6614473682133716E-5	0.0	0.1914361193839652	2.4921710523200576E-5
56-57	0.11851934801150087	1.0384046051333573E-4	2.0768092102667146E-5	0.0	0.1926302846798686	2.4921710523200576E-5
58-59	0.11868133912990167	1.121476973544026E-4	2.4921710523200576E-5	0.0	0.19374137760736126	2.4921710523200576E-5
60-61	0.11889940409697969	1.1630131577493602E-4	2.4921710523200576E-5	0.0	0.1945139506335805	2.4921710523200576E-5
62-63	0.11911954587326795	1.2253174340573616E-4	2.4921710523200576E-5	0.0	0.19546097563346213	2.4921710523200576E-5
64-65	0.11915692843905275	1.3083898024680304E-4	2.4921710523200576E-5	0.0	0.19639138615966162	2.4921710523200576E-5
66-67	0.11925246166272502	1.4745345392893673E-4	2.4921710523200576E-5	0.0	0.19710788533720364	2.4921710523200576E-5
68-69	0.11931476593903302	1.4953026313920345E-4	2.4921710523200576E-5	0.0	0.19790953369236658	2.699851973346729E-5
70-71	0.11941029916270529	1.6199111840080373E-4	2.4921710523200576E-5	0.0	0.19864887777122153	2.9075328943734005E-5
72-73	0.11945391215612089	1.6822154603160388E-4	2.4921710523200576E-5	0.0	0.19920753944878328	3.322894736426743E-5
74-75	0.11947468024822355	1.7237516445213732E-4	2.4921710523200576E-5	0.0	0.19998634290263329	3.322894736426743E-5
76-77	0.11950790919558782	1.7860559208293745E-4	2.9075328943734005E-5	0.0	0.20078799125779623	3.322894736426743E-5
78-79	0.11956605985347529	1.9314325655480448E-4	3.322894736426743E-5	0.0	0.20177239882346265	3.322894736426743E-5
80-81	0.1196159032745217	2.118345394472049E-4	3.945937499506758E-5	0.0	0.20263012102730282	3.5305756574534143E-5
82-83	0.1196470554126757	2.2014177628827176E-4	4.568980262586772E-5	0.0	0.20348161280351218	3.738256578480086E-5
84-85	0.11967197712319891	2.3052582233960533E-4	4.568980262586772E-5	0.0	0.20430818286919833	3.738256578480086E-5
86-87	0.1196968988337221	2.3260263154987204E-4	4.568980262586772E-5	6.230427630800143E-6	0.20503714290200192	3.738256578480086E-5
88-89	0.1197155901166145	2.3260263154987204E-4	4.984342104640115E-5	8.307236841066858E-6	0.20600493599398623	3.738256578480086E-5
90-91	0.11973843501792744	2.3675624997040548E-4	5.399703946693458E-5	8.307236841066858E-6	0.20662590194785596	3.945937499506758E-5
92-93	0.11974466544555823	2.3675624997040548E-4	5.399703946693458E-5	8.307236841066858E-6	0.20726555918461811	4.568980262586772E-5
94-95	0.1197778943929225	2.3675624997040548E-4	6.230427630800144E-5	8.307236841066858E-6	0.20799867283584228	4.984342104640115E-5
96-97	0.11978412482055331	2.3675624997040548E-4	6.230427630800144E-5	8.307236841066858E-6	0.20870894158575348	5.192023025666786E-5
98-99	0.11980696972186623	2.409098683909389E-4	6.230427630800144E-5	8.307236841066858E-6	0.20933613796725403	6.022746709773472E-5
100-101	0.1198277378139689	2.429866776012056E-4	6.438108551826816E-5	8.307236841066858E-6	0.21000279372374964	6.645789472853488E-5
102-103	0.11984435228765103	2.450634868114723E-4	6.645789472853486E-5	8.307236841066858E-6	0.21060091477630646	7.476513156960172E-5
104-105	0.11987342761659477	2.450634868114723E-4	6.645789472853486E-5	8.307236841066858E-6	0.21126134010517128	8.099555920040188E-5
106-107	0.11989419570869744	2.512939144422725E-4	6.645789472853486E-5	8.307236841066858E-6	0.21175769750642504	8.930279604146872E-5
108-109	0.1199211942284309	2.533707236525392E-4	7.06115131490683E-5	8.307236841066858E-6	0.21229974471030463	9.96868420928023E-5
110-111	0.11995442317579516	2.596011512833393E-4	7.06115131490683E-5	8.307236841066858E-6	0.21290824980891276	1.0176365130306901E-4
112-113	0.11996896084026704	2.6583157891413946E-4	7.06115131490683E-5	8.307236841066858E-6	0.21358113599303918	1.1630131577493602E-4
114-115	0.11997934488631837	2.6583157891413946E-4	7.06115131490683E-5	8.307236841066858E-6	0.2141854874732268	1.2460855261600288E-4
116-117	0.12000011297842103	2.6583157891413946E-4	7.06115131490683E-5	8.307236841066858E-6	0.2147649172428912	1.2876217103653632E-4
118-119	0.12001257383368263	2.679083881244062E-4	7.06115131490683E-5	8.307236841066858E-6	0.21538172957834045	1.3706940787760316E-4
120-121	0.12001880426131344	2.699851973346729E-4	7.06115131490683E-5	8.307236841066858E-6	0.21602138681510258	1.4745345392893673E-4
122-123	0.12002295787973397	2.720620065449396E-4	7.2688322359335E-5	8.307236841066858E-6	0.21654889635451036	1.6199111840080376E-4
124-125	0.12002918830736478	2.7413881575520633E-4	8.307236841066858E-5	8.307236841066858E-6	0.21728408681494477	1.6614473682133717E-4
126-127	0.12003957235341611	2.7829243417573977E-4	8.307236841066858E-5	8.307236841066858E-6	0.21795904980828143	1.7237516445213732E-4
128-129	0.1200458027810469	2.8659967101680664E-4	8.307236841066858E-5	8.307236841066858E-6	0.21849071296610972	1.7860559208293745E-4
130-131	0.12004995639946744	2.9490690785787346E-4	8.7225986831202E-5	8.307236841066858E-6	0.21921759618970305	1.8275921050347089E-4
132-133	0.12007072449157011	2.9490690785787346E-4	9.137960525173544E-5	1.2460855261600288E-5	0.2197824882948956	1.8275921050347089E-4
134-135	0.12009149258367277	2.9490690785787346E-4	9.137960525173544E-5	1.2460855261600288E-5	0.2203328427356163	1.9729687497533791E-4
136-137	0.12012056791261651	2.969837170681402E-4	9.137960525173544E-5	1.6614473682133716E-5	0.2208375073737111	2.0352730260613804E-4
138-139	0.12012264472182678	2.990605262784069E-4	9.137960525173544E-5	1.869128289240043E-5	0.22142939799863712	2.0560411181640473E-4
>>END_MODULE
