FastQCFastQC Report
Tue 31 Mar 2026
A_Ctrl_2_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA_Ctrl_2_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22039417
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCTTCAAACTACTCTACTAGCTATTAGTAAATCCTTCTAACCCTCGG645470.2928707233952695No Hit
CAGCAAGAGCTTCTTCAAACTACTCTACTAGCTATTAGTAAATCCTTCTA560650.2543851318753123No Hit
GCAAGAGCTTCTTCAAACTACTCTACTAGCTATTAGTAAATCCTTCTAAC542810.24629054389233618No Hit
AGCTTCTTCAAACTACTCTACTAGCTATTAGTAAATCCTTCTAACCCTCG294510.13362876159564474No Hit
CCACAGGTCACCAGGATACTACGATGGACGCTACTGGACCATGTGGAAGC273240.12397787110248878No Hit
CAAATGGCTTCATCCATTCTCTCATCCGCCGCTGTTGCCTCTGTCAACAG258440.11726262995069245No Hit
GTTCGAGACTCTTTCTTACCTTCCTGATATGAGTAACGAGCAATTGGGAA245420.11135503266715267No Hit
CGCTACTGGACCATGTGGAAGCTGCCCATGTTTGGCTGCACCGATTCATC243240.1103658957947935No Hit
AAAAAAAAGAGGCATCAAATGGCTTCATCCATTCTCTCATCCGCCGCTGT238520.10822427834638276No Hit
GTGGAAGCTGCCCATGTTTGGCTGCACCGATTCATCTCAGGTGGTTAATG233920.10613710879920281No Hit
CAAAAACAAGAAGAAAAATGGGCAGCACCACTGCATTCACCTCCCCATTT231050.10483489649476663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)