FastQCFastQC Report
Mon 25 May 2026
At.hfr1.00h.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAt.hfr1.00h.2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22669750
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG427960.188780202693016No Hit
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC417760.18428081474211228No Hit
CAAATCTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCG258790.11415653017788022No Hit
GAAGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCT257720.11368453555950109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)