FastQCFastQC Report
Mon 25 May 2026
At.hfr1.01h.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAt.hfr1.01h.1.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23046911
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG395460.17158915570073577No Hit
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC364630.15821209185040025No Hit
CAAATCTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCG258310.11208009611353123No Hit
GAAGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCT249410.10821840723036592No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA240810.10448688763539721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)