FastQCFastQC Report
Mon 25 May 2026
At.wt.00h.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAt.wt.00h.2.R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22719752
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG411110.18094827795655516No Hit
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC388900.17117264308166744No Hit
CAAATCTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCG293190.12904630297020847No Hit
GAAGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCT265340.11678824663226958No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA229420.10097821490305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)