FastQCFastQC Report
Mon 25 May 2026
At.wt.00h.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAt.wt.00h.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22503137
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length59-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG405800.18033041348857273No Hit
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC402910.17904614809926278No Hit
CAAATCTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCG291480.1295286075003676No Hit
GAAGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCT253990.11286870803835039No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA241330.10724282574469506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)