FastQCFastQC Report
Mon 25 May 2026
At.wt.00h.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAt.wt.00h.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23206700
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length58-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG402590.17348007256525055No Hit
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC392790.169257154183921No Hit
CAAATCTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCG285720.12311961631770135No Hit
GAAGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCT277890.11974559071302684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)