FastQCFastQC Report
Mon 25 May 2026
Ch.sis1.01h.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCh.sis1.01h.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21380400
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length58-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAATATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTCGCCTCTC771250.3607275822716133No Hit
GAAAAAGTAGTAGTAATGGCTTCCTCTATGCTCTCCTCCGCCGCTGTGGT413100.19321434584946962No Hit
AGAAAAAGTAGTAGTAATGGCTTCCTCTATGCTCTCCTCCGCCGCTGTGG336750.15750406914744344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)