##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24077270
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.77034850711895	37.0	35.0	38.0	32.0	39.0
2	35.24337564017848	35.0	34.0	38.0	32.0	39.0
3	35.46536081540806	36.0	35.0	38.0	33.0	39.0
4	35.46348672420088	36.0	35.0	38.0	32.0	39.0
5	35.37711027869854	36.0	35.0	38.0	33.0	39.0
6	35.28224678296169	36.0	35.0	37.0	33.0	39.0
7	35.14841412668463	35.0	34.0	37.0	33.0	39.0
8	35.31880424981736	35.0	34.0	37.0	33.0	39.0
9	35.28627091028177	35.0	34.0	37.0	33.0	39.0
10-14	35.32458306111947	35.8	34.2	37.4	33.0	39.0
15-19	35.3028396491795	36.0	34.0	37.0	33.0	39.0
20-24	35.30587339843761	36.0	34.0	37.0	33.0	39.0
25-29	35.293348922033104	36.0	34.0	37.0	32.6	39.0
30-34	35.26955857537005	36.0	34.0	37.0	32.0	39.0
35-39	35.23931698236553	36.0	34.0	37.0	32.0	39.0
40-44	35.221044860982985	36.0	34.0	37.0	32.0	39.0
45-49	35.20503456579588	36.0	34.0	37.0	32.0	39.0
50-54	35.18016676309232	36.0	34.0	37.0	32.0	39.0
55-59	35.15549294417515	36.0	34.0	37.0	32.0	39.0
60-64	35.13746848376083	36.0	34.0	37.0	32.0	39.0
65-69	35.10269663462677	35.8	34.0	37.0	32.0	39.0
70-74	35.09131413154399	35.8	34.0	37.0	32.0	39.0
75-79	35.061324817971474	35.2	34.0	37.0	32.0	39.0
80-84	35.040527460131486	35.4	34.0	37.0	32.0	39.0
85-89	35.01102718040708	35.0	34.0	37.0	32.0	39.0
90-94	34.98237163930961	35.0	34.0	37.0	32.0	39.0
95-99	34.94555805537753	35.0	34.0	37.0	32.0	39.0
100-104	34.91939199917599	35.0	34.0	37.0	31.8	39.0
105-109	34.892634414117545	35.0	34.0	37.0	31.2	39.0
110-114	34.85107037467287	35.0	34.0	37.0	31.0	39.0
115-119	34.82427817605567	35.0	34.0	37.0	31.0	39.0
120-124	34.78651663581461	35.0	34.0	37.0	31.0	39.0
125-129	34.742990762657065	35.0	34.0	37.0	31.0	39.0
130-134	34.708387396079374	35.0	34.0	37.0	31.0	39.0
135-139	34.666835849745425	35.0	34.0	37.0	31.0	39.0
140-144	34.616143914987035	35.0	34.0	37.0	30.6	39.0
145-149	34.5809703508745	35.0	34.0	37.0	30.0	39.0
150	34.549204207952144	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	97.0
23	1079.0
24	7833.0
25	35457.0
26	106203.0
27	225782.0
28	365468.0
29	497930.0
30	645553.0
31	846631.0
32	1143388.0
33	1621160.0
34	2479959.0
35	4450428.0
36	1.0471231E7
37	1179044.0
38	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.211201641669525	12.263358241385443	14.677073132966617	36.848366983978416
2	22.524866115754104	12.590792545062035	34.10560107175511	30.778740267428752
3	21.230895236180753	16.610635578771703	27.60320486968743	34.555264315360105
4	24.5120893943254	23.731640922514856	22.373231376230663	29.383038306929077
5	24.630302698037514	30.580596607652154	24.36297432459766	20.42612636971267
6	18.975758598731513	37.68149361162091	25.300849485711684	18.041898303935895
7	15.00323689435738	26.477679550241344	39.96042960505486	18.55865395034641
8	17.28062206157535	26.533276305429116	31.78206413712082	24.40403749587471
9	18.031215428722465	24.715692126632693	33.02686190860116	24.226230536043676
10-14	19.075697804072004	30.815802299163135	27.496848288074343	22.611651608690515
15-19	19.304052511207384	29.121067385007425	28.416809432246193	23.158070671539
20-24	19.341267088741173	29.25901180153496	28.078972106453577	23.32074900327029
25-29	19.525664252203423	29.290302243824456	27.88711732336972	23.296916180602402
30-34	19.361681622680234	29.253552525315417	27.85401667264854	23.5307491793558
35-39	19.463798729808207	29.051288342587988	28.049467647851227	23.435445279752578
40-44	19.4310838567536	29.16204200226815	27.96227587954777	23.444598261430485
45-49	19.597339534312844	28.97177401033418	27.978267631501975	23.452618823851004
50-54	19.55343663607133	29.092894581447347	27.94697404258379	23.40669473989754
55-59	19.635953618818018	28.984022300470723	27.91475741539636	23.465266665314903
60-64	19.63769246423355	28.911503041103536	27.85435995920757	23.596444535455344
65-69	19.62197370882539	28.791626134950693	28.032960129633487	23.553440026590426
70-74	19.689124305579973	28.786977861998913	27.99721742315545	23.526680409265666
75-79	19.539484706308766	28.816734331292597	28.022035725490436	23.621745236908204
80-84	19.793362492581796	28.658832340656502	27.867421532334795	23.680383634426903
85-89	19.725268455612355	28.699761134389405	28.023164766783754	23.551805643214482
90-94	19.90653784532967	28.58278155113173	27.9459240318007	23.5647565717379
95-99	19.93455084694494	28.620435224183648	27.95122690020181	23.493787028669605
100-104	19.9777565968806	28.480878485670974	27.9337612088689	23.60760370857952
105-109	19.911221721053685	28.49174727695542	28.0037843871213	23.59324661486959
110-114	19.97752085923018	28.53746599051649	27.891899868916948	23.593113281336375
115-119	20.057757955237836	28.47870661440585	27.973228357170242	23.490307073186063
120-124	20.059955889286023	28.304470882253817	28.105386555525662	23.530186672934498
125-129	20.16111425621946	28.34281488458777	27.98167312190314	23.514397737289634
130-134	20.065986121468516	28.458222096907033	27.93209213040854	23.543699651215906
135-139	20.133493320798554	28.332105256871102	27.94653249746495	23.587868924865393
140-144	20.232959375033055	28.23695329478147	28.037523196432673	23.492564133752804
145-149	20.205053662153073	28.45415817154457	28.026573601104904	23.314214565197453
150	20.183971221955467	28.365497313562965	27.790331817046837	23.66019964743473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.5
5	3.0
6	3.5
7	5.0
8	12.0
9	17.0
10	26.5
11	53.0
12	101.5
13	186.0
14	302.5
15	462.0
16	868.0
17	1636.5
18	3413.0
19	6556.0
20	8051.5
21	9692.5
22	13839.0
23	19846.5
24	29165.5
25	41211.5
26	54730.5
27	73197.5
28	100303.5
29	136673.5
30	180437.0
31	232258.5
32	286928.0
33	344759.5
34	414166.5
35	498421.0
36	593954.0
37	702531.5
38	824763.0
39	961129.0
40	1106036.5
41	1248417.5
42	1382613.0
43	1493388.0
44	1575597.5
45	1610810.5
46	1570193.5
47	1477959.0
48	1338377.5
49	1165177.5
50	984831.5
51	809380.5
52	654494.5
53	527629.5
54	433734.5
55	343657.5
56	257126.5
57	181643.0
58	118469.5
59	75594.0
60	52099.0
61	39496.0
62	29290.0
63	19443.0
64	12243.5
65	8235.0
66	5476.5
67	3684.0
68	2330.0
69	1505.0
70	1481.0
71	1905.0
72	2043.5
73	1433.5
74	823.5
75	497.0
76	251.5
77	110.0
78	36.5
79	15.0
80	4.5
81	1.5
82	4.0
83	5.5
84	5.5
85	4.0
86	2.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013165944477924617
2	5.316217328625712E-4
3	6.645271660782139E-4
4	8.971116742055888E-4
5	5.814612703184373E-4
6	4.734756058307275E-4
7	7.434397670500019E-4
8	6.977535243821247E-4
9	8.389655471737452E-4
10-14	8.157070963610076E-4
15-19	6.420993742230743E-4
20-24	5.4325095826894E-4
25-29	7.168586804068733E-4
30-34	3.638286234278222E-4
35-39	3.6216730551262663E-4
40-44	1.1380027719089414E-4
45-49	1.7277706318033565E-4
50-54	2.1098737522983295E-4
55-59	3.0817447326877175E-4
60-64	2.3341516708497268E-4
65-69	1.437039996644138E-4
70-74	8.057391888698345E-5
75-79	1.7194640422273788E-4
80-84	6.14687628622348E-5
85-89	2.051727625266486E-4
90-94	6.811403452301694E-5
95-99	5.897678598944149E-5
100-104	7.891260097178791E-5
105-109	1.2127620780927406E-4
110-114	6.313008077743033E-5
115-119	1.353974100884361E-4
120-124	1.4785729445240264E-4
125-129	8.888050846296112E-5
130-134	1.046630286573187E-4
135-139	2.093260573146374E-4
140-144	0.029412803029579347
145-149	8.888050846296112E-5
150	1.0798566448770979E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.407727E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.55382942607942
#Duplication Level	Percentage of total
1	37.10375420791989
2	11.015294608337864
3	6.345674393281703
4	4.386925980440418
5	3.250148798146731
6	2.4970126508127626
7	2.041520007687056
8	1.6975919039798892
9	1.3932602685440427
>10	16.43625683390085
>50	4.4378407728097615
>100	6.813326979847716
>500	1.4634371807692554
>1k	1.0960927913819367
>5k	0.021862622139940276
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	2.076647393994419E-5	1.0383236969972094E-4	1.3705872800363165E-4
2	0.0	4.153294787988838E-6	0.0	2.4919768727933024E-5	0.0014744196497360374	3.530300569790512E-4
3	1.661317915195535E-5	4.153294787988838E-6	0.0	2.9073063515921863E-5	0.002707948201768722	4.983953745586606E-4
4	1.661317915195535E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.004269587042052525	6.229942181983256E-4
5	1.661317915195535E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.0061759493497394015	6.686804608662029E-4
6	1.661317915195535E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.0071810466884327006	6.89446934806147E-4
7	1.661317915195535E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.008335662639493598	7.143667035340801E-4
8	1.661317915195535E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.010009440439053099	7.68359535777935E-4
9	2.076647393994419E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.013377762512112045	8.472721367497229E-4
10-11	2.076647393994419E-5	4.153294787988838E-6	0.0	3.737965309189954E-5	0.01779686816653217	9.594110960254214E-4
12-13	2.076647393994419E-5	4.153294787988838E-6	0.0	4.153294787988838E-5	0.021248256135350894	0.00109023988184707
14-15	2.076647393994419E-5	4.153294787988838E-6	0.0	4.153294787988838E-5	0.025042290924178697	0.0012003021937287742
16-17	2.2843121333938608E-5	4.153294787988838E-6	0.0	5.191618484986047E-5	0.02856843819918122	0.001308287858216484
18-19	3.737965309189954E-5	4.153294787988838E-6	0.0	5.399283224385489E-5	0.031511047556471314	0.0014079669331282158
20-21	4.5686242667877217E-5	4.153294787988838E-6	0.0	6.229942181983257E-5	0.03457825575740107	0.0014993394184639702
22-23	5.191618484986047E-5	4.153294787988838E-6	0.0	6.229942181983257E-5	0.03744195251371937	0.001601095140769697
24-25	5.8146127031843725E-5	4.153294787988838E-6	0.0	6.229942181983257E-5	0.04002322522445444	0.0017277706318033566
26-27	5.8146127031843725E-5	4.153294787988838E-6	0.0	6.229942181983257E-5	0.04355560244163894	0.0018129131749571275
28-29	6.022277442583815E-5	4.153294787988838E-6	0.0	6.437606921382699E-5	0.047056829947913534	0.0018669060072009826
30-31	6.229942181983257E-5	4.153294787988838E-6	0.0	6.852936400181581E-5	0.050707576066555723	0.0019333587238088037
32-33	6.437606921382699E-5	4.153294787988838E-6	0.0	8.306589575977674E-5	0.05386200345803324	0.002055880920054475
34-35	7.475930618379908E-5	4.153294787988838E-6	0.0	8.721919054776559E-5	0.057352847727337855	0.00218047976369414
36-37	7.475930618379908E-5	4.153294787988838E-6	2.076647393994419E-6	8.721919054776559E-5	0.06028092055286999	0.0022386258907259836
38-39	7.475930618379908E-5	4.153294787988838E-6	4.153294787988838E-6	8.721919054776559E-5	0.06328998262676791	0.002300925312545816
40-41	7.475930618379908E-5	4.153294787988838E-6	4.153294787988838E-6	8.721919054776559E-5	0.06640080042297153	0.002369454676547632
42-43	7.475930618379908E-5	4.153294787988838E-6	4.153294787988838E-6	9.760242751773767E-5	0.0692811103584418	0.0024379840405494477
44-45	7.475930618379908E-5	4.153294787988838E-6	4.153294787988838E-6	9.96790749117321E-5	0.07217803347306401	0.002523126583703219
46-47	7.68359535777935E-5	4.153294787988838E-6	4.153294787988838E-6	1.0383236969972094E-4	0.07509572306162618	0.002616575716432968
48-49	7.891260097178791E-5	4.153294787988838E-6	4.153294787988838E-6	1.0798566448770977E-4	0.07908911600027743	0.0026851050804347835
50-51	8.514254315377117E-5	4.153294787988838E-6	4.153294787988838E-6	1.204455488516763E-4	0.08215009425902522	0.0027245613809206773
52-53	9.137248533575443E-5	4.153294787988838E-6	4.153294787988838E-6	1.204455488516763E-4	0.0848746556399459	0.0027889374501345043
54-55	9.137248533575443E-5	4.153294787988838E-6	4.153294787988838E-6	1.204455488516763E-4	0.08767812962183835	0.0028450069297723535
56-57	9.137248533575443E-5	4.153294787988838E-6	4.153294787988838E-6	1.329054332156428E-4	0.09070588152228222	0.0028927698198342256
58-59	9.137248533575443E-5	4.153294787988838E-6	4.153294787988838E-6	1.329054332156428E-4	0.09310440926234578	0.002979989010381991
60-61	9.137248533575443E-5	4.153294787988838E-6	4.153294787988838E-6	1.329054332156428E-4	0.09562130590386701	0.003052671669171796
62-63	9.344913272974886E-5	6.229942181983256E-6	4.153294787988838E-6	1.329054332156428E-4	0.09810289953969034	0.0031544273914775223
64-65	9.760242751773767E-5	8.306589575977675E-6	4.153294787988838E-6	1.329054332156428E-4	0.1007589315566092	0.0032354166398433047
66-67	9.96790749117321E-5	8.306589575977675E-6	4.153294787988838E-6	1.329054332156428E-4	0.10331736114601033	0.0032852561772991704
68-69	1.0175572230572652E-4	8.306589575977675E-6	4.153294787988838E-6	1.3498208060963723E-4	0.10573042541783184	0.003366245425664953
70-71	1.121389592756986E-4	8.306589575977675E-6	4.153294787988838E-6	1.432886701856149E-4	0.10803965731995363	0.0034140083157268245
72-73	1.329054332156428E-4	8.306589575977675E-6	4.153294787988838E-6	1.453653175796093E-4	0.11011630471394805	0.0035178406854265453
74-75	1.453653175796093E-4	8.306589575977675E-6	4.153294787988838E-6	1.453653175796093E-4	0.11245253303219177	0.0035925999916103443
76-77	1.53671907155587E-4	1.2459884363966512E-5	4.153294787988838E-6	1.453653175796093E-4	0.1151251782282626	0.003663206003006155
78-79	1.53671907155587E-4	1.661317915195535E-5	4.153294787988838E-6	1.453653175796093E-4	0.11775421382905953	0.003702662303492049
80-81	1.5782520194357582E-4	1.661317915195535E-5	4.153294787988838E-6	1.453653175796093E-4	0.12060129740622588	0.0037483485461599257
82-83	1.6197849673156467E-4	1.661317915195535E-5	4.153294787988838E-6	1.453653175796093E-4	0.12317634017477895	0.003758731783129898
84-85	1.640551441255591E-4	2.076647393994419E-5	4.153294787988838E-6	1.4951861236759816E-4	0.12589467161351764	0.00378780484664582
86-87	1.661317915195535E-4	2.076647393994419E-5	4.153294787988838E-6	1.4951861236759816E-4	0.12908855530548108	0.003848027621071658
88-89	1.7028508630754233E-4	2.076647393994419E-5	4.153294787988838E-6	1.4951861236759816E-4	0.13174666396979393	0.0039061737481035017
90-91	1.765150284895256E-4	2.076647393994419E-5	4.153294787988838E-6	1.4951861236759816E-4	0.1342033378368893	0.003945630048589395
92-93	1.868982654594977E-4	2.076647393994419E-5	8.306589575977675E-6	1.4951861236759816E-4	0.13645442361197926	0.0040183127073792
94-95	1.9105156024748653E-4	2.076647393994419E-5	8.306589575977675E-6	1.4951861236759816E-4	0.13839816557275803	0.0040681522448350665
96-97	1.9520485503547538E-4	2.076647393994419E-5	8.306589575977675E-6	1.4951861236759816E-4	0.14037098059705272	0.004097225308350988
98-99	1.993581498234642E-4	2.9073063515921863E-5	8.306589575977675E-6	1.4951861236759816E-4	0.14213820752934198	0.0041449881984128596
100-101	1.993581498234642E-4	2.9073063515921863E-5	8.306589575977675E-6	1.5159525976159258E-4	0.14404456983702885	0.004176137909322777
102-103	1.993581498234642E-4	2.9073063515921863E-5	8.306589575977675E-6	1.53671907155587E-4	0.1459260123759878	0.004238437331142609
104-105	1.993581498234642E-4	3.1149710909916285E-5	8.306589575977675E-6	1.53671907155587E-4	0.14754164404851544	0.004298660105568447
106-107	1.993581498234642E-4	3.32263583039107E-5	8.306589575977675E-6	1.682084389135479E-4	0.14934002069171465	0.0043547295852062965
108-109	2.0351144461145304E-4	3.32263583039107E-5	8.306589575977675E-6	1.993581498234642E-4	0.1513626752534652	0.00439418588569219
110-111	2.0351144461145304E-4	3.32263583039107E-5	8.306589575977675E-6	2.1597132897541955E-4	0.15350785201146144	0.004450255365330039
112-113	2.0351144461145304E-4	3.32263583039107E-5	8.306589575977675E-6	2.2427791855139724E-4	0.1553166118916306	0.0044917883132099285
114-115	2.0351144461145304E-4	3.32263583039107E-5	1.2459884363966512E-5	2.2635456594539163E-4	0.15690940044282428	0.004518784729331855
116-117	2.0766473939944188E-4	3.32263583039107E-5	1.2459884363966512E-5	2.2843121333938606E-4	0.158493882404442	0.004533321261089816
118-119	2.0766473939944188E-4	3.32263583039107E-5	1.2459884363966512E-5	2.3881445030935817E-4	0.1601759667935775	0.0045810841511516875
120-121	2.097413867934363E-4	3.32263583039107E-5	1.2459884363966512E-5	2.554276294613135E-4	0.1618206715296211	0.004630923688607553
122-123	2.1597132897541957E-4	3.32263583039107E-5	1.2459884363966512E-5	2.5958092424930234E-4	0.16418181961659273	0.004659996752123476
124-125	2.2427791855139724E-4	3.32263583039107E-5	1.2459884363966512E-5	2.6165757164329676E-4	0.16589505371663815	0.004707759642185347
126-127	2.2427791855139724E-4	3.32263583039107E-5	1.2459884363966512E-5	2.6996416121927445E-4	0.1674816123256499	0.004728526116125292
128-129	2.2635456594539163E-4	3.32263583039107E-5	1.2459884363966512E-5	2.6996416121927445E-4	0.16923430272618117	0.004757599179641213
130-131	2.2843121333938606E-4	3.737965309189954E-5	1.2459884363966512E-5	2.741174560072633E-4	0.17089977393616468	0.004780442300975152
132-133	2.2843121333938606E-4	3.737965309189954E-5	1.661317915195535E-5	2.741174560072633E-4	0.17231189416408088	0.004805362069703085
134-135	2.2843121333938606E-4	3.737965309189954E-5	1.661317915195535E-5	2.741174560072633E-4	0.17396282884230646	0.004830281838431018
136-137	2.325845081273749E-4	3.737965309189954E-5	1.661317915195535E-5	2.761941034012577E-4	0.17618484155388048	0.0048635081967349295
138-139	2.325845081273749E-4	3.737965309189954E-5	1.661317915195535E-5	2.7827075079525214E-4	0.17776101692592225	0.00489258126025085
>>END_MODULE
