##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24077270
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.21493433433275	37.0	35.0	38.0	34.0	39.0
2	35.60040880880598	36.0	35.0	38.0	33.0	39.0
3	35.672871758301504	36.0	35.0	37.0	34.0	39.0
4	35.809253416188795	36.0	35.0	38.0	34.0	39.0
5	35.70786227009956	36.0	35.0	38.0	33.0	39.0
6	35.671480819877004	36.0	35.0	37.0	33.0	39.0
7	35.575490618330065	35.0	35.0	37.0	33.0	39.0
8	35.76167331263054	36.0	35.0	38.0	33.0	39.0
9	35.745942210225664	36.0	35.0	38.0	33.0	39.0
10-14	35.71498057711692	36.0	35.0	37.8	33.0	39.0
15-19	35.66895610673469	36.0	35.0	38.0	33.0	39.0
20-24	35.63918038880654	36.0	35.0	38.0	33.0	39.0
25-29	35.596070194004554	36.0	35.0	38.0	33.0	39.0
30-34	35.566497115329106	36.0	35.0	38.0	33.0	39.0
35-39	35.5346109255742	36.0	35.0	38.0	33.0	39.0
40-44	35.49696477216894	36.0	35.0	38.0	33.0	39.0
45-49	35.45241565177448	36.0	35.0	38.0	33.0	39.0
50-54	35.409117728048074	36.0	34.2	38.0	32.6	39.0
55-59	35.374865306573376	36.0	34.0	38.0	32.0	39.0
60-64	35.31959984666035	36.0	34.0	38.0	32.0	39.0
65-69	35.26569883545768	36.0	34.0	38.0	32.0	39.0
70-74	35.20698143934092	36.0	34.0	38.0	32.0	39.0
75-79	35.14705994491901	36.0	34.0	38.0	32.0	39.0
80-84	35.08383376520677	36.0	34.0	38.0	32.0	39.0
85-89	34.998607533163025	36.0	34.0	38.0	31.0	39.0
90-94	34.926968123877835	35.6	34.0	38.0	31.0	39.0
95-99	34.863407454416546	35.2	34.0	37.6	31.0	39.0
100-104	34.80658695109537	35.0	34.0	37.4	31.0	39.0
105-109	34.68953713606235	35.0	34.0	37.0	30.0	39.0
110-114	34.616114675791735	35.0	34.0	37.0	30.0	39.0
115-119	34.52536393868574	35.0	34.0	37.0	30.0	39.0
120-124	34.45853601342677	35.0	34.0	37.0	30.0	39.0
125-129	34.319800625236994	35.0	34.0	37.0	29.0	39.0
130-134	34.22449938884267	35.0	34.0	37.0	29.0	39.0
135-139	34.10784775018098	35.0	34.0	37.0	28.2	39.0
140-144	33.96074971124218	35.0	33.0	37.0	28.0	39.0
145-149	33.85183084294855	35.0	33.0	37.0	27.6	39.0
150	33.75637715571575	35.0	33.0	37.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	50.0
21	624.0
22	4447.0
23	18842.0
24	52235.0
25	109008.0
26	187684.0
27	291307.0
28	398647.0
29	499467.0
30	618409.0
31	781024.0
32	1023325.0
33	1421130.0
34	2169963.0
35	3915861.0
36	1.1012709E7
37	1572534.0
38	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.41447888402631	20.43650712892284	16.965702506970267	28.18331148008059
2	23.669128601373828	25.810604773713962	33.20464072546431	17.3156258994479
3	20.430754815641475	29.14309637263693	31.715173688711385	18.710975123010208
4	23.676700057772333	33.192272213585674	24.1423467029277	18.988681025714293
5	24.93315862748611	35.20859731766138	23.636268814107954	16.221975240744555
6	19.84185084106296	39.0631246814942	23.945783720496554	17.149240756946284
7	20.485025918636126	21.659482158899245	39.07515677649501	18.780335145969623
8	22.161582272408793	23.771860347954732	30.389612277471656	23.676945102164822
9	23.39033453543529	23.103952399919095	30.184564113788646	23.32114895085697
10-14	22.84774395103764	29.614235334819938	26.909218528512575	20.628802185629848
15-19	22.797371961189953	28.417787398654415	28.49603713377804	20.288803506377594
20-24	22.730923397876918	28.64451576113073	28.209478067903877	20.41508277308848
25-29	22.830840690437736	28.59511566872481	28.128147842250556	20.445895798586896
30-34	22.817236339501946	28.41080404879789	28.34459388460569	20.427365727094475
35-39	22.92200070855209	28.459548777747646	28.153068848752373	20.465381664947895
40-44	23.1368340347556	28.175512423127707	28.18854712349033	20.499106418626365
45-49	23.17312801658992	28.11338993166584	28.20287515984993	20.510606891894305
50-54	23.188496868623396	28.11553137045853	28.163098225006404	20.532873535911673
55-59	23.32524243820001	27.9130283458216	28.210281315115875	20.551447900862517
60-64	23.17361893603386	27.851093583284154	28.308868904157325	20.666418576524663
65-69	23.174816746250716	27.906227740935748	28.370318561863535	20.54863695095
70-74	23.399810692823564	27.820983857389148	28.260075166329074	20.519130283458217
75-79	23.25641486763242	27.83978997618916	28.351514935040395	20.552280221138027
80-84	23.426395102102525	27.90784337260827	28.126340735473747	20.53942078981546
85-89	23.40532294566618	27.86314561409994	28.27080312676645	20.46072831346743
90-94	23.3851919258288	27.85834191334815	28.263721759152926	20.492744401670123
95-99	23.295119421761687	27.9831409457966	28.312248855455792	20.409490776985926
100-104	23.521195716956285	27.91512326771266	28.142185555089927	20.421495460241132
105-109	23.287230016419887	27.981661774625294	28.40421965118321	20.326888557771614
110-114	23.39525702041801	27.951768618286042	28.28068547638499	20.372288884910954
115-119	23.47117841848349	27.945169032867927	28.35685773345566	20.22679481519292
120-124	23.527920731876996	27.949119646870262	28.39605320702887	20.12690641422387
125-129	23.461718874274368	28.167665187955276	28.230245372502775	20.140370565267574
130-134	23.61694494433962	28.13552367024999	28.175529866965814	20.072001518444573
135-139	23.413841353276347	28.165375891868138	28.40790754101275	20.012875213842765
140-144	23.376562209918315	28.23267006600001	28.262504843780047	20.128262880301627
145-149	23.55260708543784	28.432085531291545	28.01284863275612	20.00245875051449
150	23.226104952928633	28.55774346510215	28.086057929325044	20.130093652644174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	1.0
4	1.0
5	1.5
6	2.0
7	3.5
8	9.0
9	18.0
10	33.0
11	56.5
12	81.5
13	111.5
14	199.5
15	341.5
16	535.5
17	858.0
18	1430.5
19	2369.5
20	3644.0
21	5577.0
22	8271.0
23	12206.5
24	17523.0
25	25778.5
26	45500.5
27	62838.5
28	76462.0
29	103867.0
30	137579.5
31	172648.0
32	220044.5
33	283193.0
34	358830.5
35	452540.5
36	570134.5
37	706427.5
38	848338.5
39	1012629.0
40	1197392.0
41	1365178.0
42	1514028.0
43	1610607.5
44	1642660.5
45	1652190.5
46	1606475.0
47	1479877.5
48	1313667.5
49	1124118.0
50	932096.5
51	765194.0
52	618185.5
53	516905.0
54	435656.0
55	343639.0
56	255352.0
57	178545.5
58	121332.5
59	78074.0
60	53578.5
61	41840.0
62	31198.0
63	20793.0
64	13825.5
65	9429.0
66	6244.0
67	4403.5
68	3366.5
69	2524.5
70	1931.0
71	1524.0
72	1178.0
73	944.5
74	1020.0
75	987.5
76	646.0
77	307.5
78	145.5
79	73.0
80	25.5
81	5.0
82	3.5
83	3.5
84	4.5
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.13407666234585566
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.05221273009772288
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.306589575977675E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.407727E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.58456597669758
#Duplication Level	Percentage of total
1	27.595074285290742
2	10.968133346340931
3	6.760954779666394
4	4.743163914485443
5	3.468436894434923
6	2.818271678509191
7	2.3057163673999947
8	1.8964970489769777
9	1.6080709205947845
>10	18.778872479102702
>50	5.602724186117466
>100	9.169597683721971
>500	1.979571650858758
>1k	2.0163909221142937
>5k	0.24487590485421845
>10k+	0.04364793753133286
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.661317915195535E-5	0.0	0.0	4.153294787988838E-6	0.0014411932914321266	0.0
2	6.229942181983257E-5	0.0	0.0	1.2459884363966512E-5	0.010703040668647235	4.153294787988838E-6
3	1.5782520194357582E-4	0.0	0.0	1.2459884363966512E-5	0.013033039044708972	8.306589575977675E-6
4	2.9488392994720747E-4	0.0	0.0	2.076647393994419E-5	0.01601095140769697	1.2459884363966512E-5
5	3.530300569790512E-4	0.0	0.0	2.076647393994419E-5	0.01954540527227547	1.2459884363966512E-5
6	4.0286959443491723E-4	0.0	0.0	2.076647393994419E-5	0.02197508272324894	1.661317915195535E-5
7	4.485558371027945E-4	0.0	0.0	2.4919768727933024E-5	0.024604118324045875	2.076647393994419E-5
8	4.983953745586606E-4	0.0	0.0	2.4919768727933024E-5	0.030078160854615163	2.076647393994419E-5
9	8.057391888698345E-4	0.0	0.0	2.4919768727933024E-5	0.041030399210541725	2.076647393994419E-5
10-11	0.0012418351416086625	0.0	0.0	2.6996416121927443E-5	0.05038569572048658	2.6996416121927443E-5
12-13	0.0012979046212465116	0.0	0.0	4.153294787988837E-5	0.057055887149996654	2.9073063515921863E-5
14-15	0.0013747405748243053	0.0	0.0	5.399283224385489E-5	0.07728866270968428	2.9073063515921863E-5
16-17	0.001403813638340227	0.0	0.0	5.60694796378493E-5	0.09516236682979425	3.32263583039107E-5
18-19	0.0016550879730135519	0.0	0.0	6.437606921382699E-5	0.1014712216127493	3.737965309189954E-5
20-21	0.0020060413825986087	0.0	0.0	7.060601139581023E-5	0.10878725038179163	3.945630048589396E-5
22-23	0.002369454676547632	0.0	0.0	7.475930618379908E-5	0.11610120250343997	4.153294787988838E-5
24-25	0.0024006043874575484	0.0	0.0	8.306589575977674E-5	0.12099586041108482	4.153294787988838E-5
26-27	0.002450443924913414	0.0	0.0	8.721919054776559E-5	0.12551464514041666	4.153294787988838E-5
28-29	0.0026954883174047555	0.0	0.0	8.721919054776559E-5	0.13070626362540272	4.153294787988838E-5
30-31	0.0029696057734120186	4.153294787988838E-6	0.0	8.721919054776559E-5	0.1341327318254935	4.5686242667877217E-5
32-33	0.0030028321317159294	4.153294787988838E-6	0.0	9.96790749117321E-5	0.13777932464934772	4.983953745586605E-5
34-35	0.003119124385779617	4.153294787988838E-6	0.0	1.0798566448770977E-4	0.14112065030628473	5.399283224385489E-5
36-37	0.0031523507440835274	4.153294787988838E-6	0.0	1.1629225406368745E-4	0.14345687862452844	6.229942181983257E-5
38-39	0.0032291866976613213	4.153294787988838E-6	0.0	1.1629225406368745E-4	0.1458180267115001	7.891260097178792E-5
40-41	0.0032811028825111815	4.153294787988838E-6	0.0	1.1836890145768187E-4	0.14818125144586575	8.929583794176001E-5
42-43	0.0034929209166986125	4.153294787988838E-6	0.0	1.2459884363966514E-4	0.15030150843513404	9.760242751773767E-5
44-45	0.003848027621071658	4.153294787988838E-6	0.0	1.2459884363966514E-4	0.15268549964343964	1.100623118817042E-4
46-47	0.004117991782290933	4.153294787988838E-6	0.0	1.2459884363966514E-4	0.1550424944356233	1.2459884363966514E-4
48-49	0.004259203805082553	4.153294787988838E-6	0.0	1.2667549103365956E-4	0.15709837535567778	1.453653175796093E-4
50-51	0.004614310509455599	4.153294787988838E-6	0.0	1.2875213842765396E-4	0.15915217962833828	1.7443838109553118E-4
52-53	0.0048884279654628614	4.153294787988838E-6	0.0	1.329054332156428E-4	0.16127866655978856	1.993581498234642E-4
54-55	0.005096092704862304	4.153294787988838E-6	0.0	1.3913537539762605E-4	0.16281123233655642	2.3050786073338048E-4
56-57	0.005262224496381857	4.153294787988838E-6	0.0	1.4121202279162047E-4	0.16428772863368646	2.699641612192744E-4
58-59	0.005339060449959651	4.153294787988838E-6	0.0	1.453653175796093E-4	0.16569777221420867	3.1772705128114607E-4
60-61	0.00549896229929722	4.153294787988838E-6	0.0	1.453653175796093E-4	0.16704551637291104	3.96639652252934E-4
62-63	0.0061240331648895405	6.229942181983256E-6	0.0	1.453653175796093E-4	0.16830811798845965	4.568624266787721E-4
64-65	0.0069069292324254366	8.306589575977675E-6	0.0	1.5782520194357585E-4	0.16931944526933496	5.10855258922627E-4
66-67	0.007455164144439964	8.306589575977675E-6	0.0	1.6197849673156467E-4	0.17047821451518383	5.586181489844987E-4
68-69	0.007885030154996808	8.306589575977675E-6	0.0	1.7859167588352002E-4	0.17148746514866509	6.084576864403647E-4
70-71	0.008148764374034098	8.306589575977675E-6	0.0	1.9105156024748653E-4	0.17245725948166052	6.936002295941359E-4
72-73	0.009020956279511754	1.2459884363966512E-5	0.0	2.0351144461145304E-4	0.1733439879188961	7.621295935959518E-4
74-75	0.00941759593176469	1.2459884363966512E-5	0.0	2.0351144461145304E-4	0.17426601936182964	7.891260097178791E-4
76-77	0.00968548344558997	1.2459884363966512E-5	0.0	2.0351144461145304E-4	0.17531887959058481	8.140457784458122E-4
78-79	0.00982669546838159	1.2459884363966512E-5	0.0	2.0351144461145304E-4	0.17657317461655744	8.618086685076838E-4
80-81	0.010050973386932987	1.2459884363966512E-5	0.0	2.0558809200544746E-4	0.1775201258282189	8.929583794176001E-4
82-83	0.010204645294088575	1.2459884363966512E-5	0.0	2.0766473939944188E-4	0.17850653334036626	9.178781481455332E-4
84-85	0.010428923212639971	1.2459884363966512E-5	0.0	2.0766473939944188E-4	0.17940779830935982	9.427979168734661E-4
86-87	0.010528602287551703	1.2459884363966512E-5	0.0	2.097413867934363E-4	0.1803402129892633	9.760242751773768E-4
88-89	0.01053690887712768	1.4536531757960931E-5	0.0	2.1181803418743073E-4	0.18129131749571276	0.0010341704022092206
90-91	0.01053690887712768	1.661317915195535E-5	0.0	2.201246237634084E-4	0.18233587113489197	0.0010881632344530755
92-93	0.010553522056279636	1.661317915195535E-5	0.0	2.2635456594539163E-4	0.18331604870485732	0.0011566925984548913
94-95	0.010553522056279636	2.076647393994419E-5	0.0	2.2843121333938606E-4	0.18422769691082086	0.0011982255463347797
96-97	0.010555598703673631	2.076647393994419E-5	0.0	2.3050786073338048E-4	0.1853262433822439	0.001225221962456707
98-99	0.010563905293249609	2.076647393994419E-5	0.0	2.4296774509734701E-4	0.18657638511342856	0.0012688315577305898
100-101	0.010574288530219581	2.076647393994419E-5	0.0	2.4504439249134144E-4	0.1877102345905495	0.001299981268640506
102-103	0.01057844182500757	2.4919768727933024E-5	0.0	2.4504439249134144E-4	0.18854920013772325	0.0013602040430663444
104-105	0.010595055004159524	2.4919768727933024E-5	0.0	2.5335098206731913E-4	0.18962905678260036	0.0014328867018561491
106-107	0.010615821478099469	2.9073063515921863E-5	0.0	2.6165757164329676E-4	0.1904700989771681	0.0014931094762819872
108-109	0.010622051420281452	3.1149710909916285E-5	0.0	2.6996416121927445E-4	0.19146688972628542	0.0015803286668297526
110-111	0.010640741246827401	3.530300569790512E-5	0.0	3.135737564931572E-4	0.1926547320356502	0.0016280915568916243
112-113	0.010657354425979357	4.153294787988838E-5	0.0	3.2603364085712376E-4	0.19362660301603962	0.0017132341000453957
114-115	0.010669814310343324	4.5686242667877217E-5	0.0	3.281102882511182E-4	0.19464000694430889	0.0017859167588352002
116-117	0.01068227419470729	4.983953745586605E-5	0.0	3.281102882511182E-4	0.19580085283755178	0.001839909591079055
118-119	0.010688504136889274	4.983953745586605E-5	0.0	3.32263583039107E-4	0.19677687711272912	0.0019208988394448373
120-121	0.010703040668647235	4.983953745586605E-5	0.0	3.384935252210903E-4	0.1978338906362723	0.0019894282034466533
122-123	0.010705117316041228	5.191618484986047E-5	0.0	3.4057017261508466E-4	0.19914633178927676	0.002062110862236458
124-125	0.010707193963435222	5.60694796378493E-5	0.0	3.447234674030735E-4	0.20019919201803194	0.0021202569892683014
126-127	0.0107155005530112	5.8146127031843725E-5	0.0	3.530300569790512E-4	0.20122713247805918	0.0022033228850280783
128-129	0.0107155005530112	5.8146127031843725E-5	0.0	3.5718335176704004E-4	0.2024398945561519	0.0022801588386058723
130-131	0.01071965384779919	5.8146127031843725E-5	0.0	3.5718335176704004E-4	0.20366719316600262	0.0023486882026076874
132-133	0.010721730495193184	5.8146127031843725E-5	0.0	3.5718335176704004E-4	0.20477612287439564	0.0024317540983674647
134-135	0.010723807142587178	5.8146127031843725E-5	0.0	3.613366465550289E-4	0.20566700460641923	0.002533509820673191
136-137	0.010736267026951145	6.229942181983257E-5	0.0	3.8002647310097864E-4	0.20686938344754202	0.002622805658614951
138-139	0.010740420321739134	6.437606921382699E-5	0.0	3.8210312049497306E-4	0.20786825084405333	0.0027079482017687223
>>END_MODULE
