##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.2.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24177239
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.688454831422234	37.0	35.0	38.0	32.0	39.0
2	35.177806779343165	35.0	34.0	38.0	32.0	39.0
3	35.39873514920376	36.0	34.0	38.0	32.0	39.0
4	35.394980253948766	36.0	35.0	38.0	32.0	39.0
5	35.320673837074615	36.0	35.0	38.0	32.0	39.0
6	35.23915816028455	36.0	34.0	37.0	33.0	39.0
7	35.08598252265281	35.0	34.0	37.0	33.0	39.0
8	35.2627165575027	35.0	34.0	37.0	33.0	39.0
9	35.22389603709505	35.0	34.0	37.0	32.0	39.0
10-14	35.26243372123674	35.4	34.2	37.4	32.4	39.0
15-19	35.24027410243163	36.0	34.0	37.0	32.0	39.0
20-24	35.240496600955964	36.0	34.0	37.0	32.0	39.0
25-29	35.23097084824285	36.0	34.0	37.0	32.0	39.0
30-34	35.204336748294544	36.0	34.0	37.0	32.0	39.0
35-39	35.17276488022474	35.8	34.0	37.0	32.0	39.0
40-44	35.15121613348819	35.8	34.0	37.0	32.0	39.0
45-49	35.137539211983636	35.8	34.0	37.0	32.0	39.0
50-54	35.11012218558124	35.2	34.0	37.0	32.0	39.0
55-59	35.08644301361293	35.0	34.0	37.0	32.0	39.0
60-64	35.06272093351934	35.0	34.0	37.0	32.0	39.0
65-69	35.02992629555426	35.0	34.0	37.0	32.0	39.0
70-74	35.0153805072614	35.0	34.0	37.0	32.0	39.0
75-79	34.983715485461346	35.0	34.0	37.0	32.0	39.0
80-84	34.96143327201257	35.0	34.0	37.0	32.0	39.0
85-89	34.92926859018104	35.0	34.0	37.0	31.8	39.0
90-94	34.90021890423468	35.0	34.0	37.0	31.0	39.0
95-99	34.85846356567017	35.0	34.0	37.0	31.0	39.0
100-104	34.829169509388564	35.0	34.0	37.0	31.0	39.0
105-109	34.80435001697258	35.0	34.0	37.0	31.0	39.0
110-114	34.75549347053235	35.0	34.0	37.0	31.0	39.0
115-119	34.729221115777534	35.0	34.0	37.0	31.0	39.0
120-124	34.68973415037176	35.0	34.0	37.0	31.0	39.0
125-129	34.64458683640428	35.0	34.0	37.0	30.8	39.0
130-134	34.60285637247495	35.0	34.0	37.0	30.2	39.0
135-139	34.55442295954472	35.0	34.0	37.0	30.0	39.0
140-144	34.5117555151769	35.0	34.0	37.0	30.0	39.0
145-149	34.46022681911694	35.0	34.0	37.0	30.0	39.0
150	34.423860640166566	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	108.0
23	1106.0
24	8410.0
25	38267.0
26	112812.0
27	240516.0
28	389465.0
29	531809.0
30	686937.0
31	897286.0
32	1207321.0
33	1699939.0
34	2581687.0
35	4548814.0
36	1.0167804E7
37	1064934.0
38	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.970889210757484	11.750963048248	15.313474303836912	36.9646734371576
2	22.350310926686685	12.650831891127309	34.2693506189043	30.729506563281706
3	21.213912792863383	17.36170298305112	27.62146246247165	33.80292176161384
4	24.501343486889798	24.783098722741308	22.10874023188886	28.60681755848003
5	24.379448248346687	31.56141771173045	24.13631708023548	19.92281695968739
6	18.82065907250982	38.098635249715876	25.05108450365236	18.02962117412194
7	14.815493604443658	26.689599185937755	40.31363500077202	18.181272208846575
8	17.455494451815515	26.304240484114345	31.76384113593782	24.47642392813232
9	18.12328783894543	24.51684754896462	33.05977636310272	24.30008824898722
10-14	19.077457333685548	30.920875380864704	27.458048827333332	22.54361845811642
15-19	19.243189956241206	29.195552284138156	28.457664290697753	23.103593468922888
20-24	19.30840919001873	29.363035136117166	28.116145230162264	23.21241044370184
25-29	19.461309052731355	29.424135303921883	27.928972608134856	23.185583035211906
30-34	19.347240012551026	29.3709434682826	27.89446835457834	23.387348164588037
35-39	19.43850573365309	29.168171324201815	28.079047988432187	23.31427495371291
40-44	19.4052730573478	29.277509046167616	28.009667422359367	23.307550474125215
45-49	19.54023208148438	29.09455926199554	28.005750707753645	23.359457948766437
50-54	19.522468002861775	29.166210623640282	27.99997194048017	23.311349433017778
55-59	19.616279305238386	29.081331373843643	27.936996885062587	23.365392435855377
60-64	19.62733993928775	29.011792259895397	27.88598188992771	23.474885910889142
65-69	19.598976460221344	28.878655406589736	28.061731001027407	23.460637132161512
70-74	19.690370244190945	28.88721259960702	28.005109207963574	23.417307948238463
75-79	19.547490539496124	28.917519094414928	28.017406614064328	23.517583752024624
80-84	19.77023151277356	28.74881512447633	27.91414441671697	23.56680894603314
85-89	19.72339921962671	28.809856407433408	28.031943287259097	23.434801085680785
90-94	19.889101393791346	28.665053302240707	27.952614899479762	23.49323040448819
95-99	19.928770469537366	28.692989991289153	27.97017900271709	23.408060536456393
100-104	19.945207771204768	28.58694258210171	27.9520500124245	23.515799634269015
105-109	19.874751295726526	28.586544578790534	28.02955636540368	23.509147760079262
110-114	19.96856893056514	28.605022881080334	27.930004932989867	23.496403255364662
115-119	20.03389076359309	28.55894254914509	27.997504657982248	23.409662029279577
120-124	20.04352133450955	28.402013702182334	28.094703506565722	23.4597614567424
125-129	20.134373135537835	28.419585125392615	28.001271412020778	23.444770327048772
130-134	20.041523228925193	28.540447807258122	27.969808862428824	23.44822010138786
135-139	20.12337819859657	28.39085990295404	27.97791307969635	23.507848818753036
140-144	20.207169899031648	28.32301802231885	28.059448208385557	23.410363870263946
145-149	20.205220502246455	28.676046793480836	27.92265891347117	23.196073790801538
150	20.16192521800489	28.496356692935205	27.722148254492556	23.619569834567347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	3.0
7	4.5
8	6.5
9	10.0
10	17.5
11	41.0
12	74.5
13	143.5
14	244.0
15	409.5
16	752.0
17	1448.5
18	3331.5
19	6809.0
20	8252.5
21	9687.0
22	14138.0
23	20374.5
24	30164.5
25	42493.5
26	56662.5
27	76872.5
28	105726.0
29	144112.0
30	190562.0
31	243650.0
32	299096.0
33	358471.5
34	426913.5
35	511174.0
36	610521.0
37	723174.0
38	848793.0
39	988022.0
40	1131274.5
41	1271260.5
42	1404064.5
43	1510246.5
44	1586213.5
45	1613181.0
46	1563876.5
47	1465985.0
48	1325027.0
49	1149604.0
50	967741.5
51	793605.0
52	639354.5
53	513301.5
54	417246.0
55	325827.5
56	243155.0
57	171496.0
58	111729.0
59	72270.0
60	50468.5
61	38320.5
62	28597.0
63	19286.5
64	12123.0
65	8186.5
66	5522.0
67	3697.0
68	2363.5
69	1595.0
70	1549.5
71	1858.5
72	1943.0
73	1412.5
74	858.5
75	485.5
76	216.5
77	81.0
78	19.0
79	5.5
80	5.5
81	4.5
82	3.0
83	3.5
84	4.5
85	4.0
86	2.0
87	1.5
88	2.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014931398907873641
2	6.493710882371638E-4
3	7.486380061842463E-4
4	9.347634773350257E-4
5	6.865961824673197E-4
6	5.873292645202375E-4
7	9.016745046859983E-4
8	5.83193142939109E-4
9	7.899992219955306E-4
10-14	8.900933642588386E-4
15-19	6.410988450749071E-4
20-24	6.477166396047125E-4
25-29	7.354024171246353E-4
30-34	3.689420450366562E-4
35-39	3.61497026190625E-4
40-44	1.6296319029646022E-4
45-49	1.6958098482626574E-4
50-54	2.5313064076506005E-4
55-59	2.4734007055148027E-4
60-64	1.7040820914249142E-4
65-69	1.8529824683455377E-4
70-74	1.1498417995537042E-4
75-79	1.588270687153318E-4
80-84	6.535072098182924E-5
85-89	1.6709931187758865E-4
90-94	7.858631004144022E-5
95-99	8.024075867389159E-5
100-104	6.12145994007008E-5
105-109	1.273925446987557E-4
110-114	6.286904803315217E-5
115-119	1.3318311491233552E-4
120-124	1.6213596598023454E-4
125-129	1.050574881606622E-4
130-134	1.0257581521198512E-4
135-139	1.406281337583667E-4
140-144	1.6296319029646022E-4
145-149	1.0340303952821081E-4
150	1.1994752585272453E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4177239E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.55521173508439
#Duplication Level	Percentage of total
1	37.08918121268688
2	11.088289458338714
3	6.188189783630409
4	4.422350314624449
5	3.129765920015898
6	2.500773234363698
7	2.0948174828658255
8	1.6793741805734743
9	1.5542881170622196
>10	16.879310984576804
>50	4.596988003626364
>100	6.719095164161843
>500	1.2081742520855123
>1k	0.8494018913878456
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	1.2408364743385296E-4	1.778532279885226E-4
2	0.0	0.0	0.0	4.1361215811284326E-6	0.0015924068087344464	2.9780075384124716E-4
3	4.1361215811284326E-6	0.0	0.0	4.1361215811284326E-6	0.0030400493621293976	3.846593070449442E-4
4	1.2408364743385298E-5	0.0	0.0	4.1361215811284326E-6	0.0047896287909467245	4.5083725234299914E-4
5	2.4816729486770595E-5	0.0	0.0	4.1361215811284326E-6	0.006555752706088565	4.756539818297697E-4
6	2.4816729486770595E-5	0.0	0.0	4.1361215811284326E-6	0.007672505532993242	5.046068328976688E-4
7	3.308897264902746E-5	0.0	0.0	4.1361215811284326E-6	0.008859572426777103	5.252874408033109E-4
8	3.308897264902746E-5	0.0	0.0	4.1361215811284326E-6	0.010638104706662328	5.83193142939109E-4
9	3.308897264902746E-5	0.0	0.0	4.1361215811284326E-6	0.013988363187376358	6.576433313994208E-4
10-11	4.136121581128432E-5	0.0	0.0	4.1361215811284326E-6	0.018728358519349543	7.83795039623838E-4
12-13	4.136121581128432E-5	0.0	0.0	4.1361215811284326E-6	0.022163407492476705	9.078786870576909E-4
14-15	4.342927660184854E-5	0.0	0.0	6.204182371692649E-6	0.02608445075138646	0.0010133497873764659
16-17	4.5497337392412756E-5	0.0	0.0	2.2748668696206378E-5	0.02945952596158726	0.001129161191648062
18-19	5.790570213579805E-5	0.0	2.0680607905642163E-6	2.4816729486770595E-5	0.032199706509084845	0.0012201558664328876
20-21	6.410988450749071E-5	0.0	4.1361215811284326E-6	3.7225094230155895E-5	0.03531006993809342	0.0013090824804271488
22-23	7.031406687918335E-5	0.0	4.1361215811284326E-6	3.7225094230155895E-5	0.038035774060057065	0.0013897368512591534
24-25	7.031406687918335E-5	0.0	4.1361215811284326E-6	3.7225094230155895E-5	0.04064773483853967	0.001501412133949621
26-27	7.031406687918335E-5	0.0	4.1361215811284326E-6	3.929315502072011E-5	0.04417998266882335	0.0015862026263627539
28-29	7.031406687918335E-5	0.0	4.1361215811284326E-6	4.136121581128432E-5	0.0479107643350012	0.0016585847540325014
30-31	7.6518249250876E-5	0.0	4.1361215811284326E-6	4.342927660184854E-5	0.05172220037201105	0.0017516474896078912
32-33	7.858631004144022E-5	0.0	4.1361215811284326E-6	4.756539818297697E-5	0.055171725770672156	0.001884003380204001
34-35	7.858631004144022E-5	0.0	4.1361215811284326E-6	4.963345897354119E-5	0.05844753406292588	0.0020432440610774457
36-37	7.858631004144022E-5	0.0	4.1361215811284326E-6	5.790570213579805E-5	0.061572373917468406	0.0021176942495377575
38-39	7.858631004144022E-5	0.0	4.1361215811284326E-6	6.204182371692648E-5	0.06458967461090159	0.0021631915869301703
40-41	7.858631004144022E-5	0.0	4.1361215811284326E-6	6.824600608861913E-5	0.06764420039856495	0.002250050140133867
42-43	7.858631004144022E-5	0.0	4.1361215811284326E-6	7.445018846031179E-5	0.07053328132298316	0.00234931705808095
44-45	8.272243162256864E-5	0.0	4.1361215811284326E-6	7.445018846031179E-5	0.07353403753009183	0.0024568562191902886
46-47	8.272243162256864E-5	8.272243162256865E-6	4.1361215811284326E-6	9.513079636595394E-5	0.07658649525696462	0.002543714772393986
48-49	8.685855320369709E-5	1.4476425533949513E-5	4.1361215811284326E-6	9.513079636595394E-5	0.08049306209034042	0.0026450497511316326
50-51	9.306273557538973E-5	1.654448632451373E-5	4.1361215811284326E-6	9.926691794708238E-5	0.08356206430353771	0.0027194999395919444
52-53	9.513079636595394E-5	1.654448632451373E-5	4.1361215811284326E-6	9.926691794708238E-5	0.08621538629783161	0.0027877459456805637
54-55	9.513079636595394E-5	1.654448632451373E-5	4.1361215811284326E-6	9.926691794708238E-5	0.08900933642588386	0.002851855830188054
56-57	9.513079636595394E-5	2.068060790564216E-5	4.1361215811284326E-6	1.1167528269046768E-4	0.09194391468769449	0.0029201018362766734
58-59	9.513079636595394E-5	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.09430357204972825	0.003002824267899242
60-61	9.513079636595394E-5	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.0969879149558806	0.0030669341524067325
62-63	9.926691794708238E-5	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.09939927383767849	0.0031724052527255077
64-65	1.0547110031877502E-4	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.10198641788667431	0.003244787380395255
66-67	1.0753916110933924E-4	2.4816729486770595E-5	4.1361215811284326E-6	1.158114042715961E-4	0.10468937333994174	0.0032964889001593604
68-69	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.1787946506216032E-4	0.10704075845881326	0.0033812793925724937
70-71	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2408364743385296E-4	0.10947486600930735	0.003435048973127163
72-73	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2615170822441717E-4	0.11172905227102234	0.0035446561950270667
74-75	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.11443407578508034	0.0036315147482307636
76-77	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.11704603656356294	0.003712169119062768
78-79	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.11981103384054731	0.0037535303348740522
80-81	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.12262359651571464	0.003809367976219286
82-83	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1252148766862916	0.003863137556773956
84-85	1.2201558664328876E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.12800262263197215	0.0039024307117946763
86-87	1.3028782980554563E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.13115227921600148	0.003978948961045552
88-89	1.3442395138667406E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1337394232649973	0.004067875575039813
90-91	1.3649201217723827E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.13640928974561572	0.004117509034013355
92-93	1.3649201217723827E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.13857454939333644	0.00419402728326423
94-95	1.3649201217723827E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.14056402387385922	0.004270545532515106
96-97	1.406281337583667E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.14251006907778013	0.004336723477813161
98-99	1.406281337583667E-4	3.308897264902746E-5	4.1361215811284326E-6	1.3235589059610984E-4	0.1441769260749749	0.004363608268090496
100-101	1.406281337583667E-4	3.5157033439591674E-5	4.1361215811284326E-6	1.3649201217723827E-4	0.14612090321810528	0.004398765301530088
102-103	1.4476425533949512E-4	4.136121581128432E-5	4.1361215811284326E-6	1.3856007296780248E-4	0.14787668682929428	0.004467011307618707
104-105	1.4476425533949512E-4	4.136121581128432E-5	4.1361215811284326E-6	1.4476425533949512E-4	0.1495683605559758	0.00449803221947717
106-107	1.4683231613005937E-4	4.342927660184854E-5	4.1361215811284326E-6	1.4476425533949512E-4	0.15131380386321203	0.0045518018000318405
108-109	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.5510455929231622E-4	0.15328673385741026	0.0045972991374242525
110-111	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.5924068087344465E-4	0.15561743836837616	0.0046986341161619
112-113	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.654448632451373E-4	0.15752625847806692	0.004775152365412776
114-115	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.6958098482626572E-4	0.1591331417123353	0.004820649702805188
116-117	1.53036498501752E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.6958098482626572E-4	0.16069659567000186	0.004837194189129702
118-119	1.53036498501752E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.7164904561682993E-4	0.16245237928119088	0.004872351222569293
120-121	1.5924068087344465E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.778532279885226E-4	0.16428054502004963	0.004921984681542835
122-123	1.6130874166400886E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.8198934956965103E-4	0.16644580466777037	0.004950937532610734
124-125	1.6130874166400886E-4	4.963345897354119E-5	4.1361215811284326E-6	1.8819353194134367E-4	0.16796789740962564	0.004967482018935248
126-127	1.654448632451373E-4	4.963345897354119E-5	4.1361215811284326E-6	1.943977143130363E-4	0.16940106353748663	0.0049819584444691966
128-129	1.7164904561682993E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.1711609832702568	0.005008843234746532
130-131	1.7578516719795838E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.17296846840120994	0.005037796085814431
132-133	1.881935319413437E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.17427548282084648	0.005072953119254022
134-135	2.0060189668472898E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.1758410048393036	0.005126722699808692
136-137	2.026699574752932E-4	4.963345897354119E-5	4.1361215811284326E-6	2.0060189668472898E-4	0.17819652607975625	0.005161879733248284
138-139	2.026699574752932E-4	4.963345897354119E-5	4.1361215811284326E-6	2.1507832221867847E-4	0.17989026786722834	0.005178424219572797
>>END_MODULE
