##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24177239
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.097542651582344	37.0	35.0	38.0	33.0	39.0
2	35.49502104024368	36.0	35.0	38.0	33.0	39.0
3	35.57115529196696	36.0	35.0	37.0	33.0	39.0
4	35.71962526407585	36.0	35.0	38.0	33.0	39.0
5	35.598954082391295	36.0	35.0	38.0	33.0	39.0
6	35.579023725579255	36.0	35.0	37.0	33.0	39.0
7	35.47427197952587	35.0	34.0	37.0	33.0	39.0
8	35.667218618304595	36.0	35.0	38.0	33.0	39.0
9	35.65015149165709	36.0	35.0	38.0	33.0	39.0
10-14	35.61022175443607	36.0	35.0	37.8	33.0	39.0
15-19	35.56035875725926	36.0	35.0	38.0	33.0	39.0
20-24	35.53130553079282	36.0	35.0	38.0	33.0	39.0
25-29	35.47999001043915	36.0	35.0	38.0	33.0	39.0
30-34	35.45450930935497	36.0	35.0	38.0	33.0	39.0
35-39	35.41881759947859	36.0	34.8	38.0	32.8	39.0
40-44	35.37711471520797	36.0	34.0	38.0	32.0	39.0
45-49	35.33416983634898	36.0	34.0	38.0	32.0	39.0
50-54	35.288519495547035	36.0	34.0	38.0	32.0	39.0
55-59	35.249711548948994	36.0	34.0	38.0	32.0	39.0
60-64	35.19423922640629	36.0	34.0	38.0	32.0	39.0
65-69	35.13552135543682	36.0	34.0	38.0	32.0	39.0
70-74	35.07333542924401	36.0	34.0	38.0	31.8	39.0
75-79	35.01022971233399	35.8	34.0	38.0	31.0	39.0
80-84	34.9451358362301	35.2	34.0	37.6	31.0	39.0
85-89	34.85148415830277	35.0	34.0	37.0	31.0	39.0
90-94	34.77757288166775	35.0	34.0	37.0	30.4	39.0
95-99	34.714849218308174	35.0	34.0	37.0	30.0	39.0
100-104	34.6573141043938	35.0	34.0	37.0	30.0	39.0
105-109	34.53060602991103	35.0	34.0	37.0	30.0	39.0
110-114	34.46166949832444	35.0	34.0	37.0	29.8	39.0
115-119	34.36742360035403	35.0	34.0	37.0	29.0	39.0
120-124	34.30648510361336	35.0	34.0	37.0	29.0	39.0
125-129	34.15904290808392	35.0	34.0	37.0	28.6	39.0
130-134	34.0663609273168	35.0	34.0	37.0	28.0	39.0
135-139	33.94849869333715	35.0	33.2	37.0	28.0	39.0
140-144	33.79940082488328	35.0	33.0	37.0	27.0	39.0
145-149	33.68631670473208	35.0	33.0	37.0	27.0	39.0
150	33.58404071697351	35.0	33.0	37.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	75.0
21	826.0
22	5439.0
23	22240.0
24	62187.0
25	127836.0
26	219012.0
27	336348.0
28	454526.0
29	559185.0
30	681292.0
31	848215.0
32	1093322.0
33	1491569.0
34	2226671.0
35	3911212.0
36	1.0657299E7
37	1479978.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.22072305278531	19.497805353208445	17.948703737428414	28.332767856577835
2	23.725835691991133	24.77287418964589	33.87182878905238	17.629461329310598
3	20.571331573468747	28.396761929681052	32.37834973629536	18.653556760554835
4	23.98094753499355	32.82344605188376	24.059380808536492	19.136225604586198
5	25.122727051259485	35.049995674598556	23.535487018658223	16.291790255483733
6	19.820460061630694	38.94258149162525	23.876713135027536	17.360245311716525
7	20.32249009078332	21.30375184693339	39.49644953255415	18.877308529729138
8	22.12782857463584	23.5834248898313	30.351232413262743	23.937514122270123
9	23.396079262814087	23.069015448786356	29.997217631012376	23.53768765738718
10-14	22.72956974119336	29.610145310636998	26.96910263409317	20.691182314076475
15-19	22.69511750287119	28.38506580507394	28.57469705287688	20.345119639177987
20-24	22.666843802966916	28.59278348532684	28.29271530963482	20.447657402071428
25-29	22.749859538151092	28.559530473547333	28.212579049238318	20.478030939063252
30-34	22.740691772125015	28.391318793680288	28.4198340430849	20.448155391109797
35-39	22.82891193655322	28.40748027514639	28.25199932879019	20.511608459510203
40-44	23.042235715997183	28.165813308955585	28.27988423326584	20.512066741781393
45-49	23.079991061014038	28.102127790522317	28.296267410848692	20.521613737614953
50-54	23.099132395548825	28.09965793116127	28.276458104057774	20.524751569232134
55-59	23.233459370608863	27.921910355438023	28.300329082241362	20.54430119171176
60-64	23.078085136189454	27.87595225410147	28.39696542686284	20.64899718284623
65-69	23.107181924288376	27.924165368924054	28.418743761436115	20.549908945351454
70-74	23.329118763312884	27.838503809305937	28.31167280928976	20.520704618091422
75-79	23.194658414056295	27.850261975736768	28.408405939156246	20.546673671050694
80-84	23.333881920925712	27.917271281472626	28.195925928514832	20.55292086908683
85-89	23.32142226827472	27.878577864081173	28.333885436629053	20.466114431015058
90-94	23.288292761634196	27.88563077860131	28.315069392332187	20.511007067432306
95-99	23.235151871559857	28.012850433417974	28.349141934693208	20.40285576032896
100-104	23.43823792286621	27.95276334076029	28.182865711010262	20.426133025363235
105-109	23.218629720291883	28.02769580099696	28.4182788613704	20.335395617340758
110-114	23.33802714197432	27.980117994449245	28.31236602326676	20.369488840309682
115-119	23.385694482200343	27.994074327462076	28.38788356592648	20.232347624411105
120-124	23.43897746140492	27.998262332601335	28.419438629861748	20.143321576132
125-129	23.368567436505053	28.193580747578334	28.28075447324651	20.157097342670106
130-134	23.509031779848808	28.166721601254803	28.236929783421505	20.087316835474887
135-139	23.310861922653782	28.203351093977275	28.440414556848282	20.045372426520665
140-144	23.268074572121325	28.279368045292518	28.320224654270902	20.132332728315255
145-149	23.465942492440927	28.59927471453626	27.94875295727523	19.986029835747583
150	23.132868893755816	28.645102941655164	28.02494941626709	20.19707874832192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	5.0
8	11.0
9	13.5
10	25.5
11	34.5
12	55.0
13	96.5
14	155.0
15	257.5
16	404.5
17	716.5
18	1247.5
19	1984.5
20	3145.5
21	5032.5
22	7563.5
23	11398.5
24	16897.5
25	25105.0
26	46339.0
27	64096.0
28	76526.0
29	104727.5
30	139607.5
31	174661.0
32	222451.5
33	286979.5
34	362024.0
35	456090.5
36	579087.0
37	720578.0
38	865269.5
39	1031418.0
40	1217218.0
41	1383192.0
42	1527350.5
43	1621955.0
44	1650048.5
45	1654338.0
46	1605521.0
47	1477791.5
48	1313718.0
49	1124883.5
50	932524.5
51	763479.0
52	614115.0
53	511211.0
54	426074.0
55	333580.0
56	248455.5
57	174555.0
58	119807.0
59	78090.0
60	53823.0
61	41354.0
62	30392.0
63	20544.0
64	14081.0
65	9597.5
66	6349.0
67	4472.0
68	3427.0
69	2547.0
70	1879.0
71	1500.5
72	1182.5
73	999.0
74	1021.0
75	938.0
76	656.0
77	323.5
78	141.5
79	63.5
80	19.0
81	6.5
82	3.0
83	2.5
84	3.0
85	1.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.16855109055256473
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.06565679397883274
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.272243162256864E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.272243162256864E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4177239E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.47574543863627
#Duplication Level	Percentage of total
1	28.586258588544133
2	10.610770363743637
3	6.874438062949128
4	4.6271530840518365
5	3.6241641052987794
6	2.7844819950436506
7	2.355134212239446
8	1.9594229309073932
9	1.6464250811256935
>10	19.313855028530416
>50	5.611225914850699
>100	8.48891200148789
>500	1.7204740644641237
>1k	1.5743530729644215
>5k	0.22293149379904745
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2408364743385298E-5	0.0	0.0	0.0	0.0014848676476251071	4.1361215811284326E-6
2	3.7225094230155895E-5	0.0	0.0	8.272243162256865E-6	0.00942208496181057	4.1361215811284326E-6
3	1.5717262008288043E-4	0.0	0.0	8.272243162256865E-6	0.011465329022888014	4.1361215811284326E-6
4	2.812562675167334E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.014207577631176166	8.272243162256865E-6
5	3.35025848071403E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.017499930409754397	8.272243162256865E-6
6	3.557064559770452E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.019985739480012584	8.272243162256865E-6
7	4.0120379336945795E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.02235573714599918	8.272243162256865E-6
8	4.5497337392412756E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.02759206706770777	8.272243162256865E-6
9	7.445018846031178E-4	4.1361215811284326E-6	0.0	1.2408364743385298E-5	0.03828394135492477	8.272243162256865E-6
10-11	0.0011891349545744244	4.1361215811284326E-6	0.0	1.2408364743385298E-5	0.04739374913736014	8.272243162256865E-6
12-13	0.0012449725959196582	4.1361215811284326E-6	0.0	2.068060790564216E-5	0.053935025417914756	8.272243162256865E-6
14-15	0.0013152866627988416	4.1361215811284326E-6	0.0	2.8952851067899026E-5	0.07290948317134144	1.0340303952821082E-5
16-17	0.001348375635447869	4.1361215811284326E-6	0.0	3.308897264902746E-5	0.0895966656904041	1.2408364743385298E-5
18-19	0.0016647889364041941	4.1361215811284326E-6	0.0	4.136121581128432E-5	0.09573053399521757	1.4476425533949513E-5
20-21	0.0020266995747529317	4.1361215811284326E-6	0.0	4.963345897354119E-5	0.10279296159499438	1.654448632451373E-5
22-23	0.002409290821007312	4.1361215811284326E-6	0.0	4.963345897354119E-5	0.10966719566282981	2.0680607905642164E-5
24-25	0.002458924279980853	6.204182371692649E-6	0.0	4.963345897354119E-5	0.1143906465084785	2.4816729486770595E-5
26-27	0.002504421617373266	1.0340303952821082E-5	0.0	4.963345897354119E-5	0.11846679432668056	3.722509423015589E-5
28-29	0.0027401805474975864	1.2408364743385298E-5	0.0	4.963345897354119E-5	0.12339705125138564	4.5497337392412756E-5
30-31	0.003073138334778425	1.2408364743385298E-5	0.0	5.3769580554669625E-5	0.1270471785467315	4.5497337392412756E-5
32-33	0.003106227307427453	1.2408364743385298E-5	0.0	6.204182371692648E-5	0.1307448712402603	4.5497337392412756E-5
34-35	0.0032509915627669476	1.2408364743385298E-5	0.0	6.204182371692648E-5	0.1340579046267442	4.963345897354119E-5
36-37	0.003279944413834847	1.2408364743385298E-5	0.0	6.617794529805492E-5	0.13625625324711393	5.3769580554669625E-5
38-39	0.0034309128515460346	1.2408364743385298E-5	0.0	6.617794529805492E-5	0.13820229845103488	6.20418237169265E-5
40-41	0.003511567222378039	1.2408364743385298E-5	0.0	7.238212766974756E-5	0.1402889717887142	6.617794529805492E-5
42-43	0.0037845512467325156	1.2408364743385298E-5	0.0	7.445018846031179E-5	0.14214195425705972	8.065437083200444E-5
44-45	0.004150598006662382	1.2408364743385298E-5	0.0	7.858631004144022E-5	0.1444478420385388	9.099467478482551E-5
46-47	0.004547665678450712	1.4476425533949513E-5	0.0	7.858631004144022E-5	0.14641663591115595	1.1374334348103189E-4
48-49	0.004721382784858105	1.654448632451373E-5	0.0	8.272243162256866E-5	0.1483027073521505	1.2615170822441717E-4
50-51	0.005124654639018128	1.654448632451373E-5	0.0	8.685855320369709E-5	0.1502466844952809	1.4890037692062355E-4
52-53	0.0053997067241631684	1.654448632451373E-5	0.0	9.513079636595394E-5	0.15227338407003382	1.881935319413437E-4
54-55	0.005662350444564824	1.654448632451373E-5	0.0	9.926691794708238E-5	0.1537934087510985	2.2335056538093535E-4
56-57	0.005883632949155195	1.654448632451373E-5	0.0	9.926691794708238E-5	0.1553423862832311	2.77120145935605E-4
58-59	0.005982899867102277	1.654448632451373E-5	0.0	9.926691794708238E-5	0.15661837979100923	3.1434524016576087E-4
60-61	0.006181433702996442	2.068060790564216E-5	0.0	9.926691794708238E-5	0.15795434706171368	3.453661520242241E-4
62-63	0.006837008973605299	2.068060790564216E-5	0.0	9.926691794708238E-5	0.15925722535976916	4.2395246206566433E-4
64-65	0.007600123405323495	2.4816729486770595E-5	0.0	1.013349787376466E-4	0.16027057514714563	4.7772204262033394E-4
66-67	0.008185384609053168	2.4816729486770595E-5	0.0	1.0340303952821081E-4	0.16133769451507676	5.066748936882329E-4
68-69	0.008758237448039455	2.4816729486770595E-5	0.0	1.0753916110933924E-4	0.1623572484848249	5.335596839655678E-4
70-71	0.009068446566624089	2.4816729486770595E-5	0.0	1.1787946506216032E-4	0.16336025796824857	6.224862979598291E-4
72-73	0.009951508524195008	2.4816729486770595E-5	0.0	1.24083647433853E-4	0.16421643513554215	6.907323040484482E-4
74-75	0.010408549958909701	2.4816729486770595E-5	0.0	1.2821976901498141E-4	0.16507054424204518	7.217532159069114E-4
76-77	0.010735303563818847	2.4816729486770595E-5	0.0	1.3028782980554563E-4	0.166110778819699	7.527741277653748E-4
78-79	0.010927633217341318	2.4816729486770595E-5	0.0	1.3442395138667406E-4	0.1673929765098488	7.734547356710169E-4
80-81	0.011225433971182565	2.4816729486770595E-5	0.0	1.406281337583667E-4	0.16847664036410442	8.230881946445581E-4
82-83	0.011399151077589958	2.4816729486770595E-5	0.0	1.4476425533949512E-4	0.16953341942808275	8.768577751992277E-4
84-85	0.01165972673720105	2.6884790277334812E-5	0.0	1.4476425533949512E-4	0.17054676921545922	9.016745046859983E-4
86-87	0.011787946506216031	2.8952851067899026E-5	0.0	1.4683231613005937E-4	0.17152703003018666	9.161509302199479E-4
88-89	0.011808627114121674	2.8952851067899026E-5	0.0	1.4890037692062358E-4	0.1724845421762179	9.595802068217963E-4
90-91	0.011814831296493368	2.8952851067899026E-5	0.0	1.5924068087344465E-4	0.17365092846209612	0.0010030094834236449
92-93	0.011837579965189573	2.8952851067899026E-5	0.0	1.6958098482626572E-4	0.17467875467500651	0.0010360984560726723
94-95	0.011845852208351831	3.102091185846325E-5	0.0	1.6958098482626572E-4	0.17564247100340946	0.0010671193679311357
96-97	0.011852056390723523	3.308897264902746E-5	0.0	1.7164904561682993E-4	0.1767840405598009	0.0011022764013707271
98-99	0.011868600877048037	3.7225094230155895E-5	2.0680607905642163E-6	1.799212887790868E-4	0.17810966752655255	0.0011436376171820115
100-101	0.01187480505941973	3.7225094230155895E-5	4.1361215811284326E-6	1.8612547115077945E-4	0.1793897971559118	0.0011891349545744244
102-103	0.01187480505941973	3.7225094230155895E-5	4.1361215811284326E-6	2.0060189668472898E-4	0.1803493773627336	0.001234632291966837
104-105	0.01188514536337255	3.7225094230155895E-5	4.1361215811284326E-6	2.1301026142811426E-4	0.18139788418354966	0.0013276950275422268
106-107	0.01191409821444045	3.7225094230155895E-5	4.1361215811284326E-6	2.2335056538093535E-4	0.18231403511376962	0.0013856007296780249
108-109	0.011951323308670606	3.7225094230155895E-5	4.1361215811284326E-6	2.29554747752628E-4	0.18341424345434976	0.0014393703102326945
110-111	0.011980276159738505	3.7225094230155895E-5	4.1361215811284326E-6	2.4403117328657752E-4	0.18477916357612215	0.0014827995868345431
112-113	0.012023705436340354	4.136121581128432E-5	4.1361215811284326E-6	2.4609923407714173E-4	0.18587316773433063	0.001540705288970341
114-115	0.012040249922664868	4.5497337392412756E-5	4.1361215811284326E-6	2.647117811922197E-4	0.18678725060376	0.0015737942616193684
116-117	0.012040249922664868	4.5497337392412756E-5	4.1361215811284326E-6	2.812562675167334E-4	0.18789366312671185	0.0016110193558495243
118-119	0.012052658287408253	4.5497337392412756E-5	4.1361215811284326E-6	2.812562675167334E-4	0.18877258896270166	0.0016792653619381436
120-121	0.012052658287408253	5.1701519764105404E-5	4.1361215811284326E-6	2.812562675167334E-4	0.18980868741877432	0.0017371710640739415
122-123	0.012060930530570509	5.376958055466962E-5	4.1361215811284326E-6	2.853923890978618E-4	0.19129975924877113	0.0018136893133248178
124-125	0.012069202773732766	5.376958055466962E-5	4.1361215811284326E-6	2.853923890978618E-4	0.19248889420334556	0.0018715950154606156
126-127	0.012073338895313895	5.376958055466962E-5	4.1361215811284326E-6	2.853923890978618E-4	0.19360564703025024	0.0019439771431303634
128-129	0.012085747260057279	5.790570213579805E-5	4.1361215811284326E-6	2.936646322601187E-4	0.1948609599301227	0.0020163592708001108
130-131	0.012096087564010101	7.031406687918335E-5	4.1361215811284326E-6	2.936646322601187E-4	0.19611213670841404	0.0020804691553076016
132-133	0.012098155624800665	7.858631004144022E-5	6.204182371692649E-6	2.936646322601187E-4	0.19739640245935444	0.002148715161396221
134-135	0.012102291746381793	8.065437083200443E-5	8.272243162256865E-6	2.936646322601187E-4	0.19838286745645356	0.0022004166811603262
136-137	0.012104359807172357	8.479049241313287E-5	8.272243162256865E-6	2.936646322601187E-4	0.1997084944232052	0.00224177789697161
138-139	0.01211056398954405	8.685855320369709E-5	8.272243162256865E-6	2.936646322601187E-4	0.20086454040513063	0.0022914113559451515
>>END_MODULE
