##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24182374
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.08554317289113	37.0	35.0	38.0	33.0	39.0
2	35.46378213321819	35.0	34.0	38.0	33.0	39.0
3	35.58024270073732	36.0	35.0	37.0	33.0	39.0
4	35.70768391060365	36.0	35.0	38.0	33.0	39.0
5	35.584151084587475	36.0	35.0	38.0	33.0	39.0
6	35.56063399730729	36.0	35.0	37.0	33.0	39.0
7	35.47184879367096	35.0	34.0	37.0	33.0	39.0
8	35.656269686342625	36.0	35.0	38.0	33.0	39.0
9	35.63166746986876	36.0	35.0	38.0	33.0	39.0
10-14	35.59663157967866	36.0	35.0	37.8	33.0	39.0
15-19	35.545437499229806	36.0	35.0	38.0	33.0	39.0
20-24	35.51224386819921	36.0	35.0	38.0	33.0	39.0
25-29	35.461012826945776	36.0	35.0	38.0	33.0	39.0
30-34	35.42857480411145	36.0	34.4	38.0	33.0	39.0
35-39	35.38971691530368	36.0	34.0	38.0	32.6	39.0
40-44	35.34505728842007	36.0	34.0	38.0	32.0	39.0
45-49	35.29661469961551	36.0	34.0	38.0	32.0	39.0
50-54	35.246866209248104	36.0	34.0	38.0	32.0	39.0
55-59	35.20936649147846	36.0	34.0	38.0	32.0	39.0
60-64	35.14696033565604	36.0	34.0	37.8	32.0	39.0
65-69	35.08667768516027	36.0	34.0	37.6	32.0	39.0
70-74	35.02037210242468	35.8	34.0	37.0	31.4	39.0
75-79	34.95333831161489	35.2	34.0	37.0	31.0	39.0
80-84	34.88152446902029	35.0	34.0	37.0	31.0	39.0
85-89	34.78552698754886	35.0	34.0	37.0	31.0	39.0
90-94	34.70973991221871	35.0	34.0	37.0	30.0	39.0
95-99	34.64238008228638	35.0	34.0	37.0	30.0	39.0
100-104	34.58059127693584	35.0	34.0	37.0	30.0	39.0
105-109	34.45287043364725	35.0	34.0	37.0	30.0	39.0
110-114	34.37566850963433	35.0	34.0	37.0	29.4	39.0
115-119	34.27865392372147	35.0	34.0	37.0	29.0	39.0
120-124	34.21199894600919	35.0	34.0	37.0	29.0	39.0
125-129	34.05784687640676	35.0	33.8	37.0	28.0	39.0
130-134	33.96075697117247	35.0	33.0	37.0	28.0	39.0
135-139	33.8367936911405	35.0	33.0	37.0	27.8	39.0
140-144	33.6796198255804	35.0	33.0	37.0	27.0	39.0
145-149	33.560373758176105	35.0	33.0	37.0	26.8	39.0
150	33.450540836065144	35.0	33.0	37.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	59.0
21	857.0
22	5500.0
23	22603.0
24	63131.0
25	129323.0
26	217981.0
27	330680.0
28	446799.0
29	561230.0
30	694615.0
31	878123.0
32	1147660.0
33	1579811.0
34	2375669.0
35	4149945.0
36	1.0323297E7
37	1255084.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.92055825453696	18.971507925565952	17.016451734639453	29.091482085257635
2	23.45682024436476	25.056187618304143	33.96939026747333	17.517601869857774
3	20.529704817235892	28.6213628157434	31.51454443637337	19.33438793064734
4	23.980416480201654	33.2964828018953	23.586844699366573	19.136256018536475
5	24.86013651795716	35.27052532112566	23.429119103999025	16.440219056918153
6	19.674933486679183	38.93774449109091	23.953086657248786	17.43423536498112
7	20.228576400315372	21.24520115353439	39.23183472391917	19.294387722231075
8	21.999258633581633	23.643274229403612	30.22640374348689	24.131063393527867
9	23.164657034913112	23.234600540046234	30.021758823182537	23.578983601858113
10-14	22.698090766440053	29.605549066439877	26.893178477845066	20.803181689275007
15-19	22.619503775766596	28.474362359956885	28.532078777708094	20.374055086568426
20-24	22.637966810041064	28.622812632043487	28.231944473276275	20.507276084639166
25-29	22.67661398708327	28.60786292147007	28.208991014377876	20.506532077068787
30-34	22.673864857106256	28.385131253035784	28.406122575062316	20.534881314795644
35-39	22.766328070188642	28.42245265084396	28.25006924464902	20.561150034318384
40-44	22.998418600258187	28.191046917064472	28.230029855629557	20.580504627047784
45-49	22.995518967658015	28.152145856316668	28.250312396954907	20.60202277907041
50-54	23.05239446757704	28.109128806866764	28.247782688728435	20.590694036827767
55-59	23.178807202128354	27.933100595773602	28.255905960729134	20.63218624136891
60-64	23.051971655057525	27.887881479295622	28.35026370860032	20.709883157046534
65-69	23.078905321702493	27.916802543869352	28.38534959388189	20.618942540546268
70-74	23.288993876283612	27.831415559117563	28.28258548974555	20.597005074853282
75-79	23.173325331913237	27.82734896085885	28.373189497441402	20.626136209786516
80-84	23.290312191846837	27.912118140261992	28.187018363044093	20.610551304847075
85-89	23.284580744636568	27.870276094481046	28.28917458641571	20.555968574466675
90-94	23.25417347362174	27.89373450265884	28.273659980612326	20.5784320431071
95-99	23.203379453150465	27.990972267652463	28.309383520410364	20.496264758786708
100-104	23.387238159495837	27.95501632718111	28.165212398087963	20.492533115235087
105-109	23.194153917180785	28.00432189167467	28.37178705756339	20.429737133581156
110-114	23.272222983566458	27.975633823213553	28.303418018429454	20.448725174790532
115-119	23.3449339865882	27.97660252857393	28.37509938747204	20.303364097365826
120-124	23.374332065164488	27.988526684766352	28.397281424892363	20.2398598251768
125-129	23.30768186779346	28.192540566943507	28.256141435906994	20.243636129356034
130-134	23.441289924636845	28.1694113241322	28.220036626676936	20.169262124554024
135-139	23.267430236584712	28.180723695696706	28.424378847171912	20.12746722054667
140-144	23.22807843431749	28.248206731067842	28.325448113572303	20.19826672104236
145-149	23.400430412663372	28.447178097568088	28.0827730147586	20.06961847500994
150	23.095362762977693	28.531156618452762	28.179541016113635	20.193939602455906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.0
7	1.0
8	5.0
9	15.0
10	24.5
11	31.5
12	46.5
13	84.0
14	144.0
15	243.0
16	412.0
17	675.0
18	1127.5
19	1844.0
20	2881.5
21	4650.0
22	7262.0
23	11036.0
24	16209.5
25	24205.5
26	44483.0
27	62178.0
28	75100.5
29	103044.5
30	137777.5
31	173541.0
32	221794.0
33	285367.5
34	359652.5
35	453849.0
36	576128.0
37	716463.5
38	861771.0
39	1026863.0
40	1209595.5
41	1375850.5
42	1522531.0
43	1620519.0
44	1653423.0
45	1656976.0
46	1608307.0
47	1484822.5
48	1320091.5
49	1128822.0
50	938943.5
51	772184.5
52	620321.5
53	512056.5
54	423642.0
55	331434.5
56	247136.5
57	174685.5
58	121555.5
59	81458.0
60	57222.0
61	43921.0
62	32303.5
63	21918.0
64	14972.0
65	10301.5
66	6947.0
67	4966.5
68	3782.5
69	2788.5
70	2118.5
71	1656.5
72	1288.0
73	1116.5
74	1208.5
75	1165.0
76	780.0
77	360.5
78	165.0
79	73.0
80	22.0
81	9.0
82	3.5
83	5.0
84	6.0
85	3.5
86	0.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.15170553561035818
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.05940111587059236
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.27048659490586E-7
55-59	8.27048659490586E-7
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.27048659490586E-7
110-114	0.0
115-119	8.27048659490586E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4182374E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.64185721878195
#Duplication Level	Percentage of total
1	28.615706122492973
2	10.88618582523556
3	6.71772813197878
4	4.715526854468253
5	3.677834598884857
6	2.8330056413735925
7	2.310970034434792
8	1.9596521573064751
9	1.6501007903896063
>10	19.66074748619363
>50	5.634385507428459
>100	8.14106317174595
>500	1.5927398434880866
>1k	1.4168878233883018
>5k	0.1874660111906792
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.13524329745293E-6	0.0	0.0	0.0	0.0012777901789129554	0.0
2	6.202864946179395E-5	0.0	0.0	4.13524329745293E-6	0.010371190190011948	0.0
3	2.6879081433444046E-4	0.0	0.0	4.13524329745293E-6	0.012368512702681713	4.13524329745293E-6
4	3.8044238336566955E-4	0.0	0.0	8.27048659490586E-6	0.014742142355419695	4.13524329745293E-6
5	4.2179481634019886E-4	0.0	0.0	1.240572989235879E-5	0.01775673471926288	4.13524329745293E-6
6	5.044996822892575E-4	0.0	0.0	1.654097318981172E-5	0.019853303071071516	4.13524329745293E-6
7	5.541226018586926E-4	0.0	0.0	2.067621648726465E-5	0.022375801482517804	4.13524329745293E-6
8	6.326922245102983E-4	0.0	0.0	2.067621648726465E-5	0.027904621771212373	4.13524329745293E-6
9	9.304297419269092E-4	0.0	0.0	2.067621648726465E-5	0.03915248354028434	4.13524329745293E-6
10-11	0.0013749683964030992	4.13524329745293E-6	0.0	2.2743838135991114E-5	0.04825208641632951	4.13524329745293E-6
12-13	0.0014039150994852698	4.13524329745293E-6	0.0	2.894670308217051E-5	0.05451284476867325	4.13524329745293E-6
14-15	0.0014700789922445167	4.13524329745293E-6	0.0	3.308194637962344E-5	0.07186225802313702	6.202864946179395E-6
16-17	0.0015093638035703193	6.202864946179395E-6	0.0	3.721718967707637E-5	0.08686905594959371	1.240572989235879E-5
18-19	0.0017657488880124012	1.240572989235879E-5	0.0	3.721718967707637E-5	0.09307605613907055	1.654097318981172E-5
20-21	0.002102771216754815	1.240572989235879E-5	0.0	3.721718967707637E-5	0.10103019662172126	1.654097318981172E-5
22-23	0.002437725923848502	1.240572989235879E-5	0.0	4.342005462325576E-5	0.10855013655813942	1.654097318981172E-5
24-25	0.002472875491876852	1.240572989235879E-5	0.0	5.375816286688809E-5	0.11393008808812567	1.8608594838538183E-5
26-27	0.0025266336547437405	1.240572989235879E-5	0.0	5.375816286688809E-5	0.1190329783171826	2.067621648726465E-5
28-29	0.0027292605763189334	1.240572989235879E-5	0.0	5.375816286688809E-5	0.12464863871512366	2.067621648726465E-5
30-31	0.0029835780391122887	1.240572989235879E-5	0.0	5.375816286688809E-5	0.1281346488148765	2.067621648726465E-5
32-33	0.0030207952287893652	1.240572989235879E-5	0.0	5.375816286688809E-5	0.1320569270825106	2.067621648726465E-5
34-35	0.003134514419469321	1.240572989235879E-5	0.0	5.789340616434102E-5	0.13534237788233694	2.894670308217051E-5
36-37	0.0031613935009027648	1.240572989235879E-5	0.0	5.789340616434102E-5	0.13757540926296152	3.1014324730896976E-5
38-39	0.0032689098266365413	1.240572989235879E-5	0.0	5.789340616434102E-5	0.13964716615498546	3.928481132580283E-5
40-41	0.0033516146925855997	1.240572989235879E-5	0.0	6.616389275924688E-5	0.14195049667166673	4.342005462325576E-5
42-43	0.00362040550692004	1.240572989235879E-5	0.0	7.029913605669981E-5	0.1440718764832601	5.5825784515614555E-5
44-45	0.0040608089180987775	1.4473351541085255E-5	0.0	7.443437935415274E-5	0.1465116700287573	6.823151440797335E-5
46-47	0.0044764008694927965	1.654097318981172E-5	0.0	7.856962265160568E-5	0.14867233465167645	9.304297419269092E-5
48-49	0.004681095412716716	1.654097318981172E-5	0.0	7.856962265160568E-5	0.15065311619115643	1.0751632573377618E-4
50-51	0.005100822607408189	1.654097318981172E-5	0.0	7.856962265160568E-5	0.15289235043672716	1.240572989235879E-4
52-53	0.005437844936150602	1.654097318981172E-5	0.0	9.097535254396446E-5	0.1550736912761336	1.5093638035703196E-4
54-55	0.005714906237079949	1.654097318981172E-5	0.0	9.924583913887032E-5	0.15683323729919982	1.7781546179047598E-4
56-57	0.005896856942167877	2.067621648726465E-5	0.0	9.924583913887032E-5	0.15850180796972207	2.129650298188259E-4
58-59	0.006000238024604202	2.067621648726465E-5	0.0	9.924583913887032E-5	0.1597465162022554	2.501822194959023E-4
60-61	0.006178053486394677	2.067621648726465E-5	0.0	1.0131346078759678E-4	0.1610884026522789	2.97737517416611E-4
62-63	0.0068479629005820525	2.067621648726465E-5	0.0	1.0338108243632325E-4	0.16239513953427404	3.618337885271314E-4
64-65	0.00768534966831627	2.067621648726465E-5	0.0	1.0544870408504971E-4	0.16349924949469394	4.0732146479911357E-4
66-67	0.008185714107308076	2.2743838135991114E-5	0.0	1.1165156903122911E-4	0.1646509147530346	4.4453865447619E-4
68-69	0.0087501748174104	2.481145978471758E-5	0.0	1.2819254222104083E-4	0.16573641611861598	4.920939523968986E-4
70-71	0.009070656172963001	2.481145978471758E-5	0.0	1.364630288159467E-4	0.1668157146192512	5.64460710102325E-4
72-73	0.00971575412736566	2.481145978471758E-5	0.0	1.426658937621261E-4	0.16775234722612428	6.409627111052041E-4
74-75	0.01014168418700331	2.481145978471758E-5	0.0	1.488687587083055E-4	0.16870345318453844	6.616389275924687E-4
76-77	0.010472503650799546	2.481145978471758E-5	0.0	1.488687587083055E-4	0.16982203649649946	7.112618471619039E-4
78-79	0.0107268211135929	2.481145978471758E-5	0.0	1.488687587083055E-4	0.17094682267340666	7.340056852978951E-4
80-81	0.011022491009360785	2.481145978471758E-5	0.0	1.5093638035703196E-4	0.17211709652658586	7.856962265160568E-4
82-83	0.0112189150659898	2.481145978471758E-5	0.0	1.5300400200575842E-4	0.17324808556843924	8.27048659490586E-4
84-85	0.011493908745270419	2.481145978471758E-5	0.0	1.5300400200575842E-4	0.1743480602855617	8.559953625727566E-4
86-87	0.011580748854516931	3.308194637962344E-5	0.0	1.5300400200575842E-4	0.17535499202849147	8.787392007087476E-4
88-89	0.011603492692652921	3.308194637962344E-5	0.0	1.5300400200575842E-4	0.1764342905291267	9.428354718192681E-4
90-91	0.01162210128749146	3.308194637962344E-5	0.0	1.5713924530321132E-4	0.177528062381303	9.945260130374296E-4
92-93	0.011624168909140186	3.308194637962344E-5	0.0	1.7161259684429658E-4	0.1786218342334793	0.0010462165542555913
94-95	0.011624168909140186	3.308194637962344E-5	0.0	1.819507050879289E-4	0.17954399348881134	0.0010937718521763
96-97	0.011628304152437638	3.308194637962344E-5	0.0	1.819507050879289E-4	0.18071426734199048	0.0011268537985559234
98-99	0.011659318477168536	3.308194637962344E-5	0.0	1.8401832673665538E-4	0.18194243460133402	0.0011847472047202645
100-101	0.011665521342114715	3.308194637962344E-5	0.0	1.9228881333156124E-4	0.18324710386168042	0.0012260996376947937
102-103	0.011667588963763442	3.308194637962344E-5	0.0	2.005592999264671E-4	0.18424162987471784	0.0012819254222104083
104-105	0.011677927072007075	3.308194637962344E-5	0.0	2.2123551641373176E-4	0.18535401032173268	0.001356359801564561
106-107	0.01168619755860198	3.308194637962344E-5	0.0	2.2743838135991115E-4	0.18628237244201087	0.001426658937621261
108-109	0.01169860328849434	3.308194637962344E-5	0.0	2.3157362465736408E-4	0.18736580618594353	0.0015010933169754136
110-111	0.011719279504981604	3.308194637962344E-5	0.0	2.4397935454972288E-4	0.18879660036686224	0.0015796629396270193
112-113	0.011766834802902313	3.308194637962344E-5	0.0	2.481145978471758E-4	0.18993792751695926	0.0016478944540349926
114-115	0.011783375776092123	3.721718967707637E-5	0.0	2.481145978471758E-4	0.1909510621248352	0.0016954497519557012
116-117	0.011789578641038303	3.721718967707637E-5	0.0	2.481145978471758E-4	0.19211513311306821	0.001738869806578957
118-119	0.011797849127633209	3.721718967707637E-5	0.0	2.543174627933552E-4	0.19307657717972604	0.0018174394292305627
120-121	0.011818525344120475	4.342005462325576E-5	0.0	2.6258794938826104E-4	0.19424478341125648	0.001867062348799998
122-123	0.011830931074012833	4.548767627198223E-5	0.0	2.708584359831669E-4	0.1955970079695236	0.0019539024580465096
124-125	0.011835066317310286	5.1690541218161624E-5	0.0	2.770613009293463E-4	0.19679622852578493	0.001989052026074859
126-127	0.011839201560607738	5.375816286688809E-5	0.0	2.853317875242522E-4	0.19786312129652778	0.0020572835404828326
128-129	0.011847472047202644	5.375816286688809E-5	2.067621648726465E-6	2.9360227411915805E-4	0.1991367762321433	0.002131717919836985
130-131	0.01185574253379755	5.375816286688809E-5	4.13524329745293E-6	2.97737517416611E-4	0.20035253776159445	0.0021751379744602414
132-133	0.01185574253379755	5.375816286688809E-5	4.13524329745293E-6	3.018727607140639E-4	0.20142149815398602	0.0022185580290834967
134-135	0.011859877777095003	5.375816286688809E-5	6.202864946179395E-6	3.0600800401151684E-4	0.20240775368042857	0.002272316191950385
136-137	0.01186194539874373	5.375816286688809E-5	8.27048659490586E-6	3.142784906064227E-4	0.20378272207683168	0.0023426153280070847
138-139	0.011864013020392456	5.789340616434102E-5	8.27048659490586E-6	3.1841373390387563E-4	0.20485168246922325	0.002400508734171426
>>END_MODULE
