##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035964
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.10751093652828	37.0	35.0	38.0	33.0	39.0
2	35.44302475240852	35.0	34.0	37.0	33.0	39.0
3	35.525557701783875	36.0	35.0	37.0	33.0	39.0
4	35.66115330344146	36.0	35.0	38.0	33.0	39.0
5	35.557829883586116	36.0	35.0	38.0	33.0	39.0
6	35.52423518357741	36.0	35.0	37.0	33.0	39.0
7	35.435336980867504	35.0	34.0	37.0	33.0	39.0
8	35.6106917118032	36.0	35.0	38.0	33.0	39.0
9	35.59747360247336	36.0	35.0	38.0	33.0	39.0
10-14	35.56724187138905	36.0	35.0	37.8	33.0	39.0
15-19	35.51676313876989	36.0	35.0	38.0	33.0	39.0
20-24	35.485245850759306	36.0	35.0	38.0	33.0	39.0
25-29	35.43942682723273	36.0	35.0	38.0	33.0	39.0
30-34	35.40691205062547	36.0	34.0	38.0	32.8	39.0
35-39	35.372360318063386	36.0	34.0	38.0	32.0	39.0
40-44	35.3316038416433	36.0	34.0	38.0	32.0	39.0
45-49	35.28437246785692	36.0	34.0	38.0	32.0	39.0
50-54	35.23795076411331	36.0	34.0	38.0	32.0	39.0
55-59	35.19649154908036	36.0	34.0	38.0	32.0	39.0
60-64	35.145331387582374	36.0	34.0	38.0	32.0	39.0
65-69	35.091195426986	36.0	34.0	37.6	32.0	39.0
70-74	35.030119482621956	35.6	34.0	37.6	31.4	39.0
75-79	34.96858607376846	35.2	34.0	37.2	31.0	39.0
80-84	34.90402045035514	35.2	34.0	37.2	31.0	39.0
85-89	34.818577178764286	35.0	34.0	37.0	31.0	39.0
90-94	34.744194774130975	35.0	34.0	37.0	30.4	39.0
95-99	34.683211382742954	35.0	34.0	37.0	30.0	39.0
100-104	34.62181470233522	35.0	34.0	37.0	30.0	39.0
105-109	34.50535618209447	35.0	34.0	37.0	30.0	39.0
110-114	34.430548814268484	35.0	34.0	37.0	29.6	39.0
115-119	34.34104711589683	35.0	34.0	37.0	29.0	39.0
120-124	34.27481030509115	35.0	34.0	37.0	29.0	39.0
125-129	34.137736984462116	35.0	34.0	37.0	28.6	39.0
130-134	34.04257571695481	35.0	34.0	37.0	28.0	39.0
135-139	33.9297990877337	35.0	33.2	37.0	28.0	39.0
140-144	33.78336904648385	35.0	33.0	37.0	27.0	39.0
145-149	33.67307939053328	35.0	33.0	37.0	27.0	39.0
150	33.5759405780438	35.0	33.0	37.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	103.0
21	1040.0
22	6346.0
23	25406.0
24	70040.0
25	141072.0
26	235034.0
27	349857.0
28	465075.0
29	567647.0
30	688572.0
31	853180.0
32	1094269.0
33	1485523.0
34	2207814.0
35	3838656.0
36	1.0514037E7
37	1492290.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.23226545022284	20.774515222272758	15.486689029822145	29.506530297682254
2	23.607507483369506	25.99482175959325	33.81060148034836	16.587069276688883
3	19.614894580471166	29.42935011884691	32.110191211802444	18.84556408887948
4	23.120662021294425	33.75366596488495	24.22367582178106	18.90199619203956
5	24.477198425269407	35.538534383431454	23.747522038888025	16.236745152411117
6	19.428602905213204	38.904177090629695	24.357604296628168	17.309615707528934
7	20.00709852952018	21.448526050380174	39.56080147232705	18.983573947772598
8	21.622307305835538	23.751437637367072	30.647578769880003	23.978676286917388
9	23.031895038617964	23.3159568719607	30.284863964682255	23.36728412473908
10-14	22.731481874411195	29.646708573868725	26.887870193182184	20.7339393585379
15-19	22.626564093705582	28.520476233031467	28.53011345831605	20.322846214946903
20-24	22.623383027200404	28.655997321347293	28.28171901072909	20.438900640723208
25-29	22.700098873500114	28.618680942626114	28.23329107695425	20.44792910691953
30-34	22.723443087200497	28.422027924488486	28.429592422421667	20.424936565889347
35-39	22.821573538718894	28.462048786560008	28.233829107083036	20.482548567638062
40-44	23.054928023689833	28.210116307380055	28.232882192700902	20.502073476229203
45-49	23.047548248948953	28.16287210282059	28.277886420532166	20.511693227698295
50-54	23.11137094397379	28.110485603989087	28.26148350030812	20.516659951729004
55-59	23.228116833591532	27.94183582568188	28.2722873107981	20.557760029928485
60-64	23.09748591735285	27.896118499761442	28.344467482144676	20.66192810074104
65-69	23.12002131472655	27.91948931193274	28.397901577818974	20.562587795521743
70-74	23.345530056543602	27.84794485463533	28.26259100737545	20.54393408144562
75-79	23.235498272505318	27.847438946072643	28.349953428121292	20.567109353300744
80-84	23.356007689144484	27.95340681988041	28.124266619803556	20.56631887117155
85-89	23.378319255262657	27.89567499768264	28.23288302478736	20.493122722267348
90-94	23.345713115562997	27.915527748335784	28.22953720516473	20.50922193093649
95-99	23.280618992439827	28.016944109252286	28.24908041965781	20.453356478650075
100-104	23.478247013516913	27.960065175667598	28.12531421664636	20.436373594169137
105-109	23.235036464524576	28.044009385269508	28.360021674187898	20.36093247601802
110-114	23.33085371570701	27.99943617822027	28.281956155367848	20.38775395070487
115-119	23.4367608472038	28.01741174183819	28.32708103573462	20.218746375223397
120-124	23.487131200562626	28.02737015249316	28.33500749127433	20.150491155669894
125-129	23.416645157231887	28.2203426498725	28.208936408791423	20.154075784104187
130-134	23.573238002852726	28.211028274131216	28.143598484337883	20.072135238678175
135-139	23.384285315121957	28.22774572303403	28.355738925220557	20.032230036623453
140-144	23.366480329226654	28.262785715605165	28.246629925057302	20.12410403011088
145-149	23.54313228294068	28.471140163132212	27.993406879790633	19.992320674136472
150	23.20905456506758	28.562228667009155	28.065231750222292	20.163485017700975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	2.0
6	2.5
7	2.0
8	7.5
9	12.5
10	22.0
11	42.5
12	66.5
13	100.5
14	177.5
15	299.0
16	518.5
17	888.5
18	1442.5
19	2327.0
20	3692.0
21	5807.5
22	8686.0
23	12634.0
24	18217.5
25	26779.0
26	43910.0
27	61099.0
28	77148.0
29	104718.5
30	138862.5
31	174846.5
32	223624.5
33	288693.0
34	365091.5
35	459210.0
36	579864.5
37	723760.5
38	869719.5
39	1031370.5
40	1212992.0
41	1377277.5
42	1519280.5
43	1613053.0
44	1641845.0
45	1642520.5
46	1592789.5
47	1466026.0
48	1297550.5
49	1105342.5
50	920614.0
51	757684.5
52	607562.5
53	501263.0
54	415554.5
55	326432.0
56	242614.0
57	170968.5
58	119004.0
59	78919.0
60	55336.5
61	43355.5
62	31839.5
63	21144.0
64	14650.5
65	10198.5
66	6723.0
67	4708.0
68	3762.5
69	2858.0
70	1987.0
71	1453.5
72	1143.5
73	941.5
74	896.5
75	859.5
76	630.0
77	313.5
78	127.0
79	57.5
80	19.5
81	9.5
82	3.5
83	1.5
84	2.0
85	2.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.13042955131735096
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.05048934172143044
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4035964E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.900844972674136
#Duplication Level	Percentage of total
1	27.749252536239094
2	10.965710804786804
3	6.835707792925094
4	4.776468289537057
5	3.6348356570000817
6	2.919396391856934
7	2.462744957280981
8	1.9486274559154957
9	1.6973151627233438
>10	19.616986687585893
>50	5.694664959433299
>100	8.312405583383931
>500	1.7378175586878795
>1k	1.4978883289012894
>5k	0.10023303033543467
>10k+	0.04994480340727636
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0802161294633325E-5	0.0	0.0	0.0	0.001651691606793886	0.0
2	5.824605162497331E-5	0.0	0.0	8.32086451785333E-6	0.012560344989699601	8.32086451785333E-6
3	2.6626766457130656E-4	0.0	0.0	8.32086451785333E-6	0.01498171656439492	8.32086451785333E-6
4	4.3268495492837316E-4	0.0	0.0	1.2481296776779995E-5	0.018355827126384446	8.32086451785333E-6
5	4.8261014203549314E-4	0.0	0.0	1.2481296776779995E-5	0.022441371604650432	8.32086451785333E-6
6	5.574979226961732E-4	0.0	0.0	1.664172903570666E-5	0.024941791392265357	8.32086451785333E-6
7	6.240648388389998E-4	0.0	0.0	1.664172903570666E-5	0.02815780552841567	8.32086451785333E-6
8	6.906317549818264E-4	0.0	0.0	1.664172903570666E-5	0.03399489198768978	8.32086451785333E-6
9	0.001106674980874493	0.0	0.0	1.664172903570666E-5	0.04476625110605092	8.32086451785333E-6
10-11	0.0017016167939010061	4.160432258926665E-6	0.0	2.0802161294633325E-5	0.05267523283027051	1.4561512906243327E-5
12-13	0.0017473815487491994	4.160432258926665E-6	0.0	3.536367420087665E-5	0.058903399921883724	1.664172903570666E-5
14-15	0.0018721945165169993	4.160432258926665E-6	0.0	4.160432258926665E-5	0.07743604541927254	1.664172903570666E-5
16-17	0.0019408416487892892	4.160432258926665E-6	0.0	4.5764754848193315E-5	0.09410065683240332	1.8721945165169992E-5
18-19	0.002284077310150739	4.160432258926665E-6	0.0	4.992518710711998E-5	0.1001436846884943	2.0802161294633325E-5
20-21	0.002683478807007699	4.160432258926665E-6	0.0	4.992518710711998E-5	0.10709160656090183	2.0802161294633325E-5
22-23	0.0031265648425833886	4.160432258926665E-6	0.0	5.200540323658331E-5	0.11412481729461735	2.0802161294633325E-5
24-25	0.0031640087329137287	4.160432258926665E-6	0.0	5.616583549550998E-5	0.11926919178278017	2.0802161294633325E-5
26-27	0.003209773487761922	4.160432258926665E-6	0.0	6.032626775443664E-5	0.1237666190546799	2.2882377424096657E-5
28-29	0.0034261159652261087	4.160432258926665E-6	0.0	6.448670001336331E-5	0.12892763527187845	3.7443890330339985E-5
30-31	0.0037693516265875585	4.160432258926665E-6	0.0	6.864713227228997E-5	0.13250560701455535	4.5764754848193315E-5
32-33	0.0038192768136946784	4.160432258926665E-6	0.0	7.07273484017533E-5	0.13669308208316505	4.784497097765665E-5
34-35	0.003979453455663355	4.160432258926665E-6	0.0	7.488778066067997E-5	0.14023985058390004	5.200540323658331E-5
36-37	0.004021057778252622	4.160432258926665E-6	0.0	7.904821291960663E-5	0.14253640919082755	6.448670001336331E-5
38-39	0.004139630097632031	4.160432258926665E-6	0.0	7.904821291960663E-5	0.1447289569912819	7.07273484017533E-5
40-41	0.004226999175069492	4.160432258926665E-6	0.0	9.56899419553133E-5	0.14712120554016472	7.280756453121664E-5
42-43	0.004528630513841675	6.2406483883899975E-6	0.0	1.0401080647316662E-4	0.14935119723094942	8.94492935669233E-5
44-45	0.004984197846194145	8.32086451785333E-6	0.0	1.0401080647316662E-4	0.1519681091218143	1.0401080647316662E-4
46-47	0.0054543266914528574	1.0401080647316662E-5	0.0	1.0401080647316662E-4	0.15439364112876855	1.1441188712048329E-4
48-49	0.005687310897952751	1.2481296776779995E-5	0.0	1.1233167099101995E-4	0.15645721552919617	1.3313383228565328E-4
50-51	0.006178241904506098	1.2481296776779995E-5	0.0	1.1649210324994662E-4	0.1585499129554363	1.476953451918966E-4
52-53	0.006525637998126475	1.2481296776779995E-5	0.0	1.3521404841511663E-4	0.16076950356557365	1.851392355222366E-4
54-55	0.006814788040121878	1.4561512906243327E-5	0.0	1.4561512906243327E-4	0.1624690401433452	2.1426226133472323E-4
56-57	0.007006167924032504	1.664172903570666E-5	0.0	1.4561512906243327E-4	0.16400007921463022	2.454655032766732E-4
58-59	0.007083135920822647	1.664172903570666E-5	0.0	1.4561512906243327E-4	0.1653855031568528	2.808291774775499E-4
60-61	0.007289077317639517	1.8721945165169992E-5	0.0	1.476953451918966E-4	0.16682085228618249	3.265939323257432E-4
62-63	0.007979709072621344	2.2882377424096657E-5	0.0	1.4977556132135994E-4	0.1682541211993827	3.806795516917899E-4
64-65	0.009125908159955641	2.496259355355999E-5	0.0	1.6017664196867662E-4	0.1694689674189893	4.264443065399832E-4
66-67	0.009849823373008878	2.7042809683023322E-5	0.0	1.664172903570666E-4	0.17068381363859592	4.680486291292498E-4
68-69	0.01050509145378983	3.328345807141332E-5	0.0	1.913798839106266E-4	0.17187577748077837	5.075727355890532E-4
70-71	0.010912813815164643	3.328345807141332E-5	0.0	1.9554031616955325E-4	0.17306982153909034	5.783000839908064E-4
72-73	0.012048611821851622	3.328345807141332E-5	0.0	1.9762053229901657E-4	0.17404960333606756	6.490274323925598E-4
74-75	0.012741323792962913	3.328345807141332E-5	0.0	1.9970074842847992E-4	0.17506682902337514	6.739900259461198E-4
76-77	0.013217693286610016	3.328345807141332E-5	0.0	2.0178096455794327E-4	0.17618806551715588	7.093537001469964E-4
78-79	0.013417394035038496	3.328345807141332E-5	0.0	2.0386118068740658E-4	0.17742163368192765	7.57198671124653E-4
80-81	0.013735667102846384	3.328345807141332E-5	0.0	2.0802161294633325E-4	0.17847630325956554	7.75920616289823E-4
82-83	0.013922886554498085	3.328345807141332E-5	0.0	2.0802161294633325E-4	0.1794248818146008	8.279260195264064E-4
84-85	0.014274443080377388	3.328345807141332E-5	0.0	2.0802161294633325E-4	0.18039218231480125	8.63289693727283E-4
86-87	0.014397175832015725	3.328345807141332E-5	0.0	2.1218204520525991E-4	0.18131371806015353	9.06974232446013E-4
88-89	0.014436699938475527	3.328345807141332E-5	0.0	2.1634247746418658E-4	0.182308061370037	9.50658771164743E-4
90-91	0.014447101019122846	3.328345807141332E-5	0.0	2.2882377424096657E-4	0.18331488597669726	0.0010089048227897163
92-93	0.014457502099770161	3.328345807141332E-5	0.0	2.4338528714720991E-4	0.18436331490594676	0.0010775519550620062
94-95	0.014461662532029088	3.328345807141332E-5	0.0	2.4546550327667326E-4	0.18532437475775884	0.0011253969260396628
96-97	0.014478304261064796	4.368453871872998E-5	0.0	2.475457194061366E-4	0.18644561125153958	0.001162840816370003
98-99	0.014509507503006745	4.5764754848193315E-5	0.0	2.5794680005345323E-4	0.18760013120339172	0.0012127660034771228
100-101	0.014542790961078158	4.784497097765665E-5	0.0	2.621072323123799E-4	0.18874425007459655	0.0012751724873610228
102-103	0.014555272257854936	4.992518710711998E-5	0.0	2.6418744844184327E-4	0.1897344329522211	0.0012980548647851195
104-105	0.014573994203020108	4.992518710711998E-5	0.0	2.7666874521862323E-4	0.1905685996201359	0.0013583811325395562
106-107	0.014586475499796888	4.992518710711998E-5	0.0	2.829093936070132E-4	0.19148805514935868	0.001399985455128823
108-109	0.014611438093350446	4.992518710711998E-5	0.0	2.870698258659399E-4	0.19249904018827785	0.0014582315067537962
110-111	0.014659283064328104	4.992518710711998E-5	0.0	3.0787198716057326E-4	0.1936202766820586	0.0015268786390260861
112-113	0.014715448899823614	4.992518710711998E-5	0.0	3.120324194194999E-4	0.19456885523709389	0.0015872049067805227
114-115	0.01473209062885932	4.992518710711998E-5	0.0	3.1619285167842654E-4	0.19539886147274976	0.0016371300938876428
116-117	0.014742491709506637	4.992518710711998E-5	0.0	3.224335000668165E-4	0.19647225299555282	0.001651691606793886
118-119	0.014748732357895027	4.992518710711998E-5	0.0	3.2451371619627987E-4	0.19745827544091846	0.0017307398197134926
120-121	0.014763293870801272	4.992518710711998E-5	0.0	3.3491479684359654E-4	0.19837357053788232	0.0017661034939143692
122-123	0.01476953451918966	5.4085619366046645E-5	0.0	3.432356613614499E-4	0.19945736314133272	0.0018555527874812927
124-125	0.01477577516757805	5.4085619366046645E-5	0.0	3.515565258793032E-4	0.20043714493830994	0.001903397758458949
126-127	0.014782015815966441	5.824605162497331E-5	0.0	3.619576065266199E-4	0.20144396954497018	0.001982445971378556
128-129	0.014790336680484295	5.824605162497331E-5	0.0	3.723586871739365E-4	0.20266505641296517	0.002034451374615139
130-131	0.014790336680484295	6.032626775443664E-5	0.0	3.7443890330339985E-4	0.2037010040454379	0.0020864567778517227
132-133	0.014790336680484295	6.240648388389997E-5	0.0	3.7443890330339985E-4	0.2047972779456651	0.0021384621810883056
134-135	0.014790336680484295	6.240648388389997E-5	0.0	3.7443890330339985E-4	0.20573961585231199	0.002207109313360596
136-137	0.014790336680484295	6.240648388389997E-5	0.0	3.7859933556232654E-4	0.20695654228804802	0.0022507938520793256
138-139	0.014794497112743221	6.448670001336331E-5	0.0	3.806795516917899E-4	0.20794464494954312	0.0022986388230569824
>>END_MODULE
