##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.2.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24119014
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.710729758687485	37.0	35.0	38.0	32.0	39.0
2	35.17905885373258	35.0	34.0	38.0	32.0	39.0
3	35.408593941692644	36.0	34.0	38.0	32.0	39.0
4	35.39035409988153	36.0	35.0	38.0	32.0	39.0
5	35.30989902821069	36.0	35.0	38.0	32.0	39.0
6	35.22330054619977	36.0	34.0	37.0	33.0	39.0
7	35.08767547462761	35.0	34.0	37.0	33.0	39.0
8	35.2621134512381	35.0	34.0	37.0	33.0	39.0
9	35.227106381711955	35.0	34.0	37.0	32.0	39.0
10-14	35.26249779530788	35.4	34.2	37.4	32.2	39.0
15-19	35.237294733524344	36.0	34.0	37.0	32.0	39.0
20-24	35.24250208569886	36.0	34.0	37.0	32.0	39.0
25-29	35.2285286703677	36.0	34.0	37.0	32.0	39.0
30-34	35.20512889954789	36.0	34.0	37.0	32.0	39.0
35-39	35.17074275092671	36.0	34.0	37.0	32.0	39.0
40-44	35.15130181524004	36.0	34.0	37.0	32.0	39.0
45-49	35.13382818219684	35.8	34.0	37.0	32.0	39.0
50-54	35.10781501267009	35.6	34.0	37.0	32.0	39.0
55-59	35.08137005103111	35.2	34.0	37.0	32.0	39.0
60-64	35.05844388995338	35.0	34.0	37.0	32.0	39.0
65-69	35.021627998557484	35.0	34.0	37.0	32.0	39.0
70-74	35.008332247744455	35.0	34.0	37.0	32.0	39.0
75-79	34.97412217597286	35.0	34.0	37.0	32.0	39.0
80-84	34.94983477351106	35.0	34.0	37.0	32.0	39.0
85-89	34.91497668188261	35.0	34.0	37.0	31.2	39.0
90-94	34.883299159741775	35.0	34.0	37.0	31.0	39.0
95-99	34.838479251266236	35.0	34.0	37.0	31.0	39.0
100-104	34.80956417206774	35.0	34.0	37.0	31.0	39.0
105-109	34.78257527442871	35.0	34.0	37.0	31.0	39.0
110-114	34.73136216928271	35.0	34.0	37.0	31.0	39.0
115-119	34.70243161681485	35.0	34.0	37.0	31.0	39.0
120-124	34.66221224466307	35.0	34.0	37.0	31.0	39.0
125-129	34.61434498939302	35.0	34.0	37.0	30.2	39.0
130-134	34.57132066841538	35.0	34.0	37.0	30.0	39.0
135-139	34.52232463565882	35.0	34.0	37.0	30.0	39.0
140-144	34.47936798743099	35.0	34.0	37.0	30.0	39.0
145-149	34.42651041207572	35.0	34.0	37.0	30.0	39.0
150	34.396718663540724	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	68.0
23	1121.0
24	8135.0
25	36838.0
26	109602.0
27	235840.0
28	386536.0
29	530211.0
30	689435.0
31	907265.0
32	1228119.0
33	1732196.0
34	2621601.0
35	4566692.0
36	9998591.0
37	1066748.0
38	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.1716155826141	11.855585277599918	14.97219848425175	37.000600655534235
2	22.353836205036153	13.059901822014808	34.38464663052536	30.201615342423676
3	21.159229915955265	17.561993699360606	27.47943494089388	33.79934144379025
4	24.3758610493014	25.17660476703097	22.012512382442466	28.43502180122517
5	24.288467201125627	31.7148755790389	24.054774428262444	19.94188279157303
6	18.66822091338046	38.16661770775698	25.104357108014824	18.060804270847736
7	14.906995568642998	26.327544445935676	40.24738878974768	18.51807119567365
8	17.4774253332974	26.216353598948537	31.706238066906174	24.599983000847885
9	18.186190857260705	24.579277176810162	32.85310468946015	24.38142727646898
10-14	19.171940289947845	30.81494836481669	27.313845619542388	22.699265725693078
15-19	19.283410029646856	29.17366299331255	28.37687283826733	23.166054138773266
20-24	19.364170880133226	29.313883423732307	28.031272923971322	23.29067277216314
25-29	19.501435650166158	29.403648810735767	27.8660160789568	23.22889946014127
30-34	19.39072831097634	29.328673314170512	27.853684044332315	23.426914330520827
35-39	19.478793735022855	29.14585444366256	28.0060363897892	23.36931543152538
40-44	19.47079135432377	29.218319386045643	27.94280268568255	23.368086573948034
45-49	19.583461662468615	29.041939882443074	27.946432938174958	23.42816551691335
50-54	19.563852561765323	29.116489048405665	27.940993626838047	23.378664762990965
55-59	19.656933124229685	29.046811312778836	27.90251926249054	23.39373630050094
60-64	19.66403147716011	28.96183004270273	27.833006726015846	23.541131754121313
65-69	19.663552060907687	28.850570734279607	28.01088046697593	23.474996737836776
70-74	19.740260420868058	28.85558286755372	27.961488999074458	23.442667712503766
75-79	19.627579119160615	28.85867655617253	27.970311481572317	23.54343284309454
80-84	19.82958564635753	28.72810504275774	27.84733225188446	23.59497705900027
85-89	19.79738626391481	28.782949863776974	27.971917946369928	23.447745925938285
90-94	19.93414866690509	28.668206470987595	27.8875487492319	23.510096112875413
95-99	19.973630081760692	28.6798823788607	27.923651952072404	23.422835587306203
100-104	20.007615905231486	28.56413236676138	27.917391558594456	23.510860169412677
105-109	19.930940871563585	28.552822362275478	27.969529130825155	23.546707635335782
110-114	20.00453915999834	28.573109996268503	27.893509681164225	23.528841162568927
115-119	20.085290675739557	28.509054454660408	27.958308085581134	23.4473467840189
120-124	20.098701073755745	28.351512027405352	28.061580048724803	23.4882068501141
125-129	20.19650755053534	28.371864200393322	27.977808757329566	23.453819491741772
130-134	20.11238714223269	28.49543248640942	27.937955503172297	23.45422486818559
135-139	20.182226763818033	28.334661291547558	27.957399926393244	23.52571201824117
140-144	20.26817308778669	28.255388962424817	28.033626636289476	23.442811313499018
145-149	20.26545737560462	28.558897248719045	27.920968401381042	23.254676974295293
150	20.237635315664754	28.424687550971278	27.673656433312626	23.664020700051335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	3.0
8	8.0
9	12.5
10	16.5
11	29.0
12	48.5
13	111.0
14	218.0
15	353.0
16	686.0
17	1309.0
18	2701.5
19	4906.0
20	6151.5
21	8135.0
22	12327.0
23	17979.0
24	26420.0
25	38321.5
26	52739.5
27	72313.0
28	100447.0
29	137433.0
30	182320.0
31	234370.0
32	289722.0
33	348713.5
34	418707.5
35	504339.0
36	602450.0
37	712235.5
38	836409.0
39	976363.5
40	1123012.0
41	1267470.5
42	1401125.5
43	1509121.0
44	1587314.5
45	1618208.5
46	1573047.0
47	1475771.0
48	1334647.0
49	1161756.5
50	981530.5
51	803685.0
52	645554.0
53	517638.0
54	419113.5
55	326301.0
56	242904.0
57	172276.5
58	114875.5
59	74783.0
60	51237.0
61	38292.0
62	28845.5
63	20049.0
64	12905.5
65	8698.0
66	5798.5
67	3774.5
68	2297.0
69	1516.0
70	1375.0
71	1539.5
72	1607.5
73	1157.5
74	678.5
75	427.5
76	217.0
77	87.0
78	28.5
79	10.5
80	6.5
81	4.5
82	3.5
83	5.5
84	6.5
85	4.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015133288616193017
2	6.841075675813282E-4
3	7.960524422764546E-4
4	7.711758034553153E-4
5	6.965458869918978E-4
6	6.177698640582903E-4
7	8.997051040312012E-4
8	6.799614611111383E-4
9	9.328739557927201E-4
10-14	8.599024819173786E-4
15-19	6.915705592276699E-4
20-24	6.484510519376952E-4
25-29	7.695173608672394E-4
30-34	3.565651564363286E-4
35-39	3.913924507859235E-4
40-44	1.4677216904472132E-4
45-49	1.7911179951220227E-4
50-54	2.4876638821139205E-4
55-59	2.753014696206072E-4
60-64	1.6667348010163268E-4
65-69	2.039884383333415E-4
70-74	1.0448188304878465E-4
75-79	1.3847995610434159E-4
80-84	5.3070162818430306E-5
85-89	1.5423516069106307E-4
90-94	7.711758034553153E-5
95-99	6.716692481707585E-5
100-104	6.799614611111383E-5
105-109	1.25212415399734E-4
110-114	5.8045490582658146E-5
115-119	1.4594294775068333E-4
120-124	1.9237934021680984E-4
125-129	9.204356363821505E-5
130-134	1.1277409598916439E-4
135-139	1.3350462834011375E-4
140-144	1.6169815233740484E-4
145-149	9.618967010840492E-5
150	1.6584425880759472E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4119014E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.81796883690809
#Duplication Level	Percentage of total
1	37.26358491698692
2	11.038080207321556
3	6.407395871419156
4	4.301444026705472
5	3.3687209028864316
6	2.650651115987356
7	2.053457692862406
8	1.6782046663920493
9	1.50898437619673
>10	17.1577724880183
>50	4.608012893994456
>100	6.268912292846385
>500	1.08175374207299
>1k	0.5893055181106666
>5k	0.02371928819908765
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.1461064701898675E-6	9.950655528455683E-5	1.285293005758859E-4
2	8.292212940379735E-6	0.0	0.0	2.4876638821139207E-5	0.0015838126716125295	3.109579852642401E-4
3	1.2438319410569603E-5	0.0	0.0	2.4876638821139207E-5	0.0028608134644310087	3.8973400819784754E-4
4	2.0730532350949337E-5	0.0	0.0	2.4876638821139207E-5	0.004564863223679045	4.933866699525942E-4
5	2.9022745291329073E-5	0.0	0.0	2.4876638821139207E-5	0.006173552534112713	5.348477346544929E-4
6	2.9022745291329073E-5	0.0	0.0	2.4876638821139207E-5	0.007226663577540939	5.597243734756321E-4
7	3.7314958231708806E-5	0.0	0.0	2.4876638821139207E-5	0.008292212940379736	5.887471187669613E-4
8	3.7314958231708806E-5	0.0	0.0	2.4876638821139207E-5	0.010087477041971948	6.302081834688598E-4
9	4.146106470189867E-5	0.0	0.0	2.4876638821139207E-5	0.013300709556369096	7.089842064024673E-4
10-11	4.5607171172088546E-5	0.0	0.0	2.4876638821139207E-5	0.01773497042623716	7.877602293360748E-4
12-13	4.5607171172088546E-5	4.1461064701898675E-6	0.0	2.4876638821139207E-5	0.021145142997968325	9.639697543191443E-4
14-15	4.768022440718348E-5	4.1461064701898675E-6	0.0	2.694969205623414E-5	0.025156501007877023	0.0011153026404810743
16-17	5.389938411246828E-5	4.1461064701898675E-6	0.0	3.938801146680374E-5	0.028516920301965912	0.0012168822490007261
18-19	5.389938411246828E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.031429560097274295	0.0013329732301660424
20-21	6.011854381775308E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.03440853759610571	0.0013930917739837954
22-23	6.633770352303788E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.037358492349645805	0.0014843061163279728
24-25	6.633770352303788E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.040202721388196055	0.001633565949254808
26-27	7.462991646341761E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.043965313009893356	0.0017538030368903141
28-29	7.877602293360748E-5	4.1461064701898675E-6	0.0	4.975327764227841E-5	0.04760559449072006	0.0018636748583503455
30-31	8.292212940379735E-5	4.1461064701898675E-6	0.0	5.597243734756321E-5	0.05131635978153999	0.0019548892006945227
32-33	8.292212940379735E-5	4.1461064701898675E-6	0.0	7.462991646341763E-5	0.054483985124765044	0.0020751262883300287
34-35	8.292212940379735E-5	4.1461064701898675E-6	0.0	8.706823587398723E-5	0.05758319971123198	0.0022036555889059146
36-37	8.292212940379735E-5	4.1461064701898675E-6	0.0	8.706823587398723E-5	0.060622295753881145	0.002292796878014997
38-39	9.536044881436696E-5	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.0636282229447688	0.002375719007418794
40-41	9.536044881436696E-5	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.06676060638299725	0.0024938830418192053
42-43	1.0365266175474669E-4	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.06973336472212338	0.002599608756809047
44-45	1.0365266175474669E-4	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.07260454345272987	0.0027115536315041734
46-47	1.0365266175474669E-4	4.1461064701898675E-6	0.0	9.950655528455683E-5	0.07551096408833297	0.002792402707672876
48-49	1.0365266175474669E-4	6.219159705284802E-6	0.0	1.1401792793022135E-4	0.07938757363796049	0.002896055369427623
50-51	1.0365266175474669E-4	8.292212940379735E-6	0.0	1.2023708763550616E-4	0.0824080122014938	0.0029665391794208504
52-53	1.0779876822493656E-4	8.292212940379735E-6	0.0	1.2023708763550616E-4	0.08514029636534892	0.0030225116167684135
54-55	1.098718214600315E-4	8.292212940379735E-6	0.0	1.2231014087060109E-4	0.08799903677654485	0.0030950684799967362
56-57	1.1194487469512643E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09091582267832342	0.003144821757639015
58-59	1.1194487469512643E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09328117641956674	0.0032360360999831917
60-61	1.1401792793022135E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09577298640815085	0.003341761814973033
62-63	1.1816403440041123E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09839954485701613	0.0034661450090787294
64-65	1.2023708763550616E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.10125206610850676	0.0035428479787772416
66-67	1.2231014087060109E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.10406105324206039	0.003611258735535375
68-69	1.285293005758859E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.10661712788093244	0.003661012013177653
70-71	1.285293005758859E-4	8.292212940379735E-6	0.0	1.534059393970251E-4	0.1091338145083377	0.0037294227699357857
72-73	1.3267540704607576E-4	1.2438319410569603E-5	0.0	1.596250991023099E-4	0.11148880298340555	0.0038123448993395833
74-75	1.3889456675136055E-4	1.2438319410569603E-5	0.0	1.6169815233740484E-4	0.11410085005962516	0.0038994131352135704
76-77	1.4925983292683525E-4	1.658442588075947E-5	0.0	1.6169815233740484E-4	0.11694093299170522	0.003980262211382273
78-79	1.5755204586721496E-4	1.658442588075947E-5	0.0	1.6169815233740484E-4	0.11977894287055019	0.0040196502228490765
80-81	1.596250991023099E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.12251330008764039	0.004065257394021166
82-83	1.6999036527778457E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.12521863455943927	0.004110864565193254
84-85	1.782825782181643E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.12802140253328764	0.004179275321951387
86-87	1.8242868468835419E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.13111232490681418	0.004266343557825373
88-89	1.8864784439363898E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.1337616869412655	0.004328535154878222
90-91	1.9486700409892377E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.13653543216982253	0.004388653698695975
92-93	2.0523227027439845E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.13867482310844048	0.004452918348983918
94-95	2.1974364292006297E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14065044284148598	0.004517182999271861
96-97	2.2388974939025285E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14258460150982954	0.004573155436619424
98-99	2.2388974939025285E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14443791110200443	0.004604251235145847
100-101	2.2803585586044273E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14649852601768878	0.004633273980437177
102-103	2.3218196233063259E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14846378048455877	0.004674735045139076
104-105	2.3425501556572753E-4	3.1095798526424007E-5	0.0	1.658442588075947E-4	0.15020099909556833	0.004728634429251545
106-107	2.3632806880082247E-4	3.316885176151894E-5	0.0	1.7620952498306937E-4	0.15195687518569373	0.004794972132774582
108-109	2.3632806880082247E-4	3.316885176151894E-5	0.0	1.8864784439363898E-4	0.15420406489253666	0.004846798463651955
110-111	2.3632806880082247E-4	3.316885176151894E-5	0.0	1.9486700409892377E-4	0.156635756337303	0.004935939752761038
112-113	2.3632806880082247E-4	3.524190499661387E-5	0.0	1.9486700409892377E-4	0.15849113898271297	0.005000204403048981
114-115	2.3632806880082247E-4	3.7314958231708806E-5	0.0	1.969400573340187E-4	0.16005214806873946	0.005037519361280688
116-117	2.5291249468158193E-4	3.7314958231708806E-5	0.0	1.9901311056911365E-4	0.16174583256181202	0.005070688213042208
118-119	2.5291249468158193E-4	4.146106470189867E-5	0.0	2.0315921703930353E-4	0.16343537094841437	0.0051038570648037265
120-121	2.5498554791667687E-4	4.5607171172088546E-5	0.0	2.0730532350949339E-4	0.16513734765442734	0.005139098969800341
122-123	2.570586011517718E-4	4.975327764227841E-5	0.0	2.093783767445883E-4	0.167401121787151	0.00517641392803205
124-125	2.5913165438686675E-4	4.975327764227841E-5	0.0	2.19743642920063E-4	0.16906992964140244	0.005205436673323379
126-127	2.736430270325312E-4	4.975327764227841E-5	0.0	2.2388974939025285E-4	0.1706309387274289	0.005226167205674329
128-129	2.8608134644310087E-4	4.975327764227841E-5	0.0	2.2803585586044273E-4	0.172641800365471	0.005255189950965657
130-131	2.8608134644310087E-4	4.975327764227841E-5	0.0	2.2803585586044273E-4	0.17432926569883828	0.005286285749492081
132-133	2.881543996781958E-4	5.8045490582658146E-5	0.0	2.2803585586044273E-4	0.17573064968576246	0.0053318929206641694
134-135	2.9022745291329075E-4	6.011854381775308E-5	0.0	2.2803585586044273E-4	0.17732897373002066	0.005373353985366069
136-137	2.9022745291329075E-4	6.426465028794295E-5	0.0	2.3010890909553765E-4	0.17943934192334726	0.0053982306241872075
138-139	2.9022745291329075E-4	6.633770352303788E-5	0.0	2.3218196233063259E-4	0.18081170316498013	0.005423107263008347
>>END_MODULE
