##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24119014
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.08513034570982	37.0	35.0	38.0	33.0	39.0
2	35.45754453312229	36.0	34.0	38.0	33.0	39.0
3	35.55651831372543	36.0	35.0	37.0	33.0	39.0
4	35.689527233575966	36.0	35.0	38.0	33.0	39.0
5	35.56534574755005	36.0	35.0	38.0	33.0	39.0
6	35.54998877648978	36.0	35.0	37.0	33.0	39.0
7	35.45441841030483	35.0	34.0	37.0	33.0	39.0
8	35.637948798404445	36.0	35.0	38.0	33.0	39.0
9	35.61757221916286	36.0	35.0	38.0	33.0	39.0
10-14	35.58162793885355	36.0	35.0	37.8	33.0	39.0
15-19	35.53025978590998	36.0	35.0	38.0	33.0	39.0
20-24	35.50111531922491	36.0	35.0	38.0	33.0	39.0
25-29	35.44841802405355	36.0	35.0	38.0	33.0	39.0
30-34	35.423038354718805	36.0	34.6	38.0	32.8	39.0
35-39	35.386032762367485	36.0	34.0	38.0	32.2	39.0
40-44	35.34362096228312	36.0	34.0	38.0	32.0	39.0
45-49	35.29996418593231	36.0	34.0	38.0	32.0	39.0
50-54	35.25338937155557	36.0	34.0	38.0	32.0	39.0
55-59	35.21325890021872	36.0	34.0	38.0	32.0	39.0
60-64	35.156545495599445	36.0	34.0	38.0	32.0	39.0
65-69	35.0977272702773	36.0	34.0	38.0	32.0	39.0
70-74	35.03352627101589	36.0	34.0	38.0	31.0	39.0
75-79	34.96848684610407	35.2	34.0	37.6	31.0	39.0
80-84	34.90306903922358	35.2	34.0	37.2	31.0	39.0
85-89	34.8070488785321	35.0	34.0	37.0	31.0	39.0
90-94	34.732308095181665	35.0	34.0	37.0	30.0	39.0
95-99	34.6676205005727	35.0	34.0	37.0	30.0	39.0
100-104	34.6079247932772	35.0	34.0	37.0	30.0	39.0
105-109	34.480148483681795	35.0	34.0	37.0	30.0	39.0
110-114	34.40668902136713	35.0	34.0	37.0	29.4	39.0
115-119	34.31177380634216	35.0	34.0	37.0	29.0	39.0
120-124	34.248258614551986	35.0	34.0	37.0	29.0	39.0
125-129	34.09848305573354	35.0	34.0	37.0	28.4	39.0
130-134	34.002896196337055	35.0	33.6	37.0	28.0	39.0
135-139	33.882649149753796	35.0	33.0	37.0	27.8	39.0
140-144	33.727774219957745	35.0	33.0	37.0	27.0	39.0
145-149	33.611835757465045	35.0	33.0	37.0	26.8	39.0
150	33.50312317908187	35.0	33.0	37.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	66.0
21	906.0
22	5913.0
23	24073.0
24	65845.0
25	133895.0
26	225448.0
27	341669.0
28	461082.0
29	570303.0
30	697123.0
31	868638.0
32	1120832.0
33	1520479.0
34	2265452.0
35	3954049.0
36	1.0434389E7
37	1428851.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.74977459692175	19.26924541774386	17.48153552214033	28.499444463194056
2	23.751974272248443	24.986199684613972	33.737336857966085	17.5244891851715
3	20.51216521537738	28.49591198048146	31.891904868084573	19.10001793605659
4	23.81891316120966	32.985821891392405	23.938437118532292	19.256827828865642
5	24.865453749240892	35.16986373306237	23.550741822459504	16.41394069523724
6	19.665729287275177	38.92938160739075	23.946580071639744	17.45830903369433
7	20.279759363297355	21.292396115363587	39.308746203306654	19.119098318032403
8	22.12479332695773	23.575880838246537	30.277319794250296	24.02200604054544
9	23.345875581812756	23.116956605274165	30.00202661684263	23.53514119607045
10-14	22.7759177883474	29.511075369830625	26.91928534060306	20.793721501218915
15-19	22.695047152425055	28.33940143655955	28.536871366300463	20.428680044714927
20-24	22.682763068175174	28.5285907624582	28.250989862189225	20.5376563071774
25-29	22.759731119582664	28.53839083100926	28.17948194277281	20.52239610663527
30-34	22.74503261202966	28.361822751129047	28.36471507500265	20.52842956183864
35-39	22.83502302374384	28.400704108385195	28.189815719664164	20.574457148206804
40-44	23.061791829466994	28.15291703052206	28.221679377108867	20.56361176290208
45-49	23.064817657968938	28.103328767917297	28.23834672511903	20.59350684899474
50-54	23.11972059156084	28.094962158029862	28.205481602236986	20.579835648172313
55-59	23.232720044028333	27.90634227419081	28.248564389904164	20.61237329187669
60-64	23.09734883855534	27.87575147143246	28.325292236241502	20.701607453770706
65-69	23.12616013241669	27.912142677142604	28.360065631206982	20.601631559233724
70-74	23.331303676012627	27.811664274501435	28.269886986259056	20.58714506322688
75-79	23.212351052161583	27.821969836743744	28.359671751092314	20.606007360002362
80-84	23.326966019423516	27.90926942535876	28.144839585896836	20.618924969320883
85-89	23.356272358397405	27.862671334740302	28.255310934352458	20.52574537250984
90-94	23.31138909741501	27.88008332347251	28.2576974332367	20.550830145875782
95-99	23.257920079154147	27.981879358749907	28.289605039409988	20.47059552268596
100-104	23.453018435994107	27.92220113143929	28.14387768919575	20.48090274337085
105-109	23.226127734740732	27.987352219290557	28.390865397731435	20.39565464823728
110-114	23.321818213630127	27.957458791640487	28.289685473875508	20.431037520853877
115-119	23.389156981202937	27.951137869492825	28.370562149601657	20.289142999702584
120-124	23.432299512741274	27.96547487388995	28.389032818671613	20.213192794697164
125-129	23.376374341007473	28.15032986008466	28.25960049610652	20.213695302801348
130-134	23.51719435960359	28.12906530922035	28.200648666649474	20.153091664526585
135-139	23.32478682586278	28.13845624037533	28.418729720874992	20.118027212886894
140-144	23.29938216628078	28.211379024129084	28.292752998051686	20.196485811538448
145-149	23.483361301585546	28.481954527660207	27.980442318247338	20.05424185250691
150	23.170710875660173	28.51828022488813	28.061375145766736	20.249633753684957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	3.0
8	3.5
9	11.5
10	21.0
11	25.5
12	43.0
13	88.5
14	129.0
15	205.5
16	359.5
17	616.5
18	1062.5
19	1725.5
20	2784.5
21	4415.5
22	6709.5
23	10241.0
24	15368.5
25	23168.5
26	39339.5
27	55115.0
28	69863.5
29	96416.5
30	129349.0
31	164284.5
32	213553.5
33	277635.0
34	352053.0
35	446723.0
36	567110.0
37	709407.5
38	858045.5
39	1023507.0
40	1207127.0
41	1376419.0
42	1525438.0
43	1623540.0
44	1657536.5
45	1664211.0
46	1618155.5
47	1493328.0
48	1325064.5
49	1134317.0
50	946035.0
51	776719.5
52	620727.0
53	510657.5
54	424783.5
55	333053.0
56	246437.0
57	173018.0
58	119453.5
59	78664.5
60	54195.5
61	41557.5
62	30465.5
63	20446.0
64	14029.5
65	9728.5
66	6563.0
67	4489.0
68	3395.0
69	2459.5
70	1752.5
71	1338.5
72	1025.5
73	855.0
74	846.5
75	784.5
76	534.5
77	262.5
78	131.0
79	62.5
80	10.0
81	2.5
82	2.5
83	2.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.17173172999526432
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.06640652889044303
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.292212940379735E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.292212940379735E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.292212940379735E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4119014E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.63970735192895
#Duplication Level	Percentage of total
1	28.520525049943085
2	10.87450940145231
3	6.920074153747689
4	4.915092737963224
5	3.6353002017801055
6	2.805336380473937
7	2.323041343999657
8	1.9675749223873513
9	1.6510729484332318
>10	19.476607962601943
>50	5.579485138456704
>100	8.24894480905232
>500	1.6041558357725594
>1k	1.4165912600266224
>5k	0.0616878539093172
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.658442588075947E-5	0.0	0.0	0.0	0.001596250991023099	4.1461064701898675E-6
2	4.146106470189867E-5	1.2438319410569603E-5	0.0	8.292212940379735E-6	0.010174545277845936	8.292212940379735E-6
3	1.9901311056911365E-4	1.2438319410569603E-5	0.0	8.292212940379735E-6	0.012052731508841945	8.292212940379735E-6
4	3.8973400819784754E-4	1.2438319410569603E-5	0.0	1.2438319410569603E-5	0.014594294775068335	8.292212940379735E-6
5	4.3948728584012594E-4	1.2438319410569603E-5	0.0	1.2438319410569603E-5	0.017616806391836746	8.292212940379735E-6
6	5.01678882892974E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.019664982988110542	8.292212940379735E-6
7	5.763087993563916E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.022073870847290854	8.292212940379735E-6
8	6.426465028794295E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.027086513569750406	8.292212940379735E-6
9	9.867733399051884E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.03710765290819931	8.292212940379735E-6
10-11	0.0014801600098577827	1.658442588075947E-5	0.0	2.4876638821139207E-5	0.044599667299832405	8.292212940379735E-6
12-13	0.0014967444357385422	1.658442588075947E-5	0.0	5.5972437347563206E-5	0.050410435517803505	8.292212940379735E-6
14-15	0.0015485707666159157	1.658442588075947E-5	0.0	7.048380999322775E-5	0.06590028929043286	8.292212940379735E-6
16-17	0.0015775935119072446	1.658442588075947E-5	0.0	7.048380999322775E-5	0.07930879761502688	8.292212940379735E-6
18-19	0.0018678209648205354	1.658442588075947E-5	0.0	7.048380999322775E-5	0.08496615989360096	8.292212940379735E-6
20-21	0.002280358558604427	1.658442588075947E-5	0.0	7.048380999322775E-5	0.09191296128440407	8.292212940379735E-6
22-23	0.0027032614185637936	1.658442588075947E-5	0.0	7.462991646341761E-5	0.0991043829569484	1.2438319410569603E-5
24-25	0.002753014696206072	1.658442588075947E-5	0.0	7.462991646341761E-5	0.10390972035589846	1.2438319410569603E-5
26-27	0.0028234985061992997	1.658442588075947E-5	0.0	7.462991646341761E-5	0.10840202671634919	1.4511372645664537E-5
28-29	0.003124091225288065	1.658442588075947E-5	0.0	7.462991646341761E-5	0.1136323400284937	1.658442588075947E-5
30-31	0.0034640719558436343	1.8657479115854403E-5	0.0	7.462991646341761E-5	0.11695959047082108	1.658442588075947E-5
32-33	0.0034993138608402485	2.0730532350949337E-5	0.0	7.462991646341761E-5	0.12079681200898179	1.658442588075947E-5
34-35	0.0036527198002372736	2.2803585586044273E-5	0.0	7.462991646341761E-5	0.12424222648570957	1.8657479115854403E-5
36-37	0.003687961705233887	2.4876638821139207E-5	0.0	8.084907616870242E-5	0.1264085671163838	2.2803585586044273E-5
38-39	0.0038061257396342987	2.4876638821139207E-5	0.0	8.706823587398723E-5	0.12843186707383644	2.9022745291329073E-5
40-41	0.003874536496392431	2.4876638821139207E-5	0.0	9.536044881436696E-5	0.13055674663980874	4.5607171172088546E-5
42-43	0.004181348375186482	2.4876638821139207E-5	0.0	1.0779876822493656E-4	0.13263601903460898	6.426465028794295E-5
44-45	0.004662296725728507	2.4876638821139207E-5	0.0	1.1609098116531629E-4	0.1349910075096768	7.255686322832269E-5
46-47	0.005091418745393157	2.4876638821139207E-5	0.0	1.347484602811707E-4	0.13729416965386726	8.914128910908215E-5
48-49	0.00529872406890265	2.4876638821139207E-5	0.0	1.471867796917403E-4	0.13912882176692629	1.1194487469512643E-4
50-51	0.005771380206504296	2.4876638821139207E-5	0.0	1.5547899263212002E-4	0.14118114446967028	1.596250991023099E-4
52-53	0.00613001841617572	2.4876638821139207E-5	0.0	1.6584425880759472E-4	0.14331224319534785	2.0523227027439845E-4
54-55	0.006455487774085624	2.4876638821139207E-5	0.0	1.7413647174797445E-4	0.14495202830430798	2.4876638821139205E-4
56-57	0.006716692481707585	2.4876638821139207E-5	0.0	1.7413647174797445E-4	0.1464902338047484	2.777891335027211E-4
58-59	0.006855587048458947	2.4876638821139207E-5	0.0	1.782825782181643E-4	0.14781284176873896	3.3168851761518944E-4
60-61	0.007096061223729959	2.4876638821139207E-5	0.0	1.8450173792344913E-4	0.14905667370979592	3.7936874202237284E-4
62-63	0.007809191536602615	2.4876638821139207E-5	0.0	1.9072089762873392E-4	0.15031916312996874	4.3119507289974624E-4
64-65	0.00876486907798138	2.4876638821139207E-5	4.1461064701898675E-6	1.9279395086382883E-4	0.15133081310869506	4.7265613760164493E-4
66-67	0.009442757485857423	2.4876638821139207E-5	4.1461064701898675E-6	1.9901311056911365E-4	0.1525456223044607	5.099710958333537E-4
68-69	0.010145522532554607	2.4876638821139207E-5	4.1461064701898675E-6	2.1974364292006297E-4	0.15359458724141872	5.410668943597777E-4
70-71	0.01051245295516641	2.4876638821139207E-5	4.1461064701898675E-6	2.218166961551579E-4	0.15467050187043302	6.053315446477207E-4
72-73	0.011505445454776882	2.4876638821139207E-5	4.1461064701898675E-6	2.2388974939025285E-4	0.15553081896299742	6.778884078760433E-4
74-75	0.012090046467073654	2.4876638821139207E-5	4.1461064701898675E-6	2.2388974939025285E-4	0.15645954681231994	7.048380999322775E-4
76-77	0.012506730167327736	2.9022745291329073E-5	4.1461064701898675E-6	2.2803585586044273E-4	0.1574546123651655	7.297147387534167E-4
78-79	0.0126850127455459	3.1095798526424007E-5	4.1461064701898675E-6	2.2803585586044273E-4	0.15852016172800432	7.773949631606001E-4
80-81	0.012971094091989	3.316885176151894E-5	4.1461064701898675E-6	2.3840112203591738E-4	0.15947998537585326	8.001985487466445E-4
82-83	0.013141084457266786	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.1602718917116595	8.375135069783532E-4
84-85	0.013393996951948367	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.16116745070922053	8.540979328591127E-4
86-87	0.013522526252524253	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.16207337497295704	8.769015184451571E-4
88-89	0.013537037625169918	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.16304563694021657	9.328739557927202E-4
90-91	0.013551548997815584	3.7314958231708806E-5	4.1461064701898675E-6	2.5913165438686675E-4	0.164038629439827	0.001005430819021043
92-93	0.013580571743106912	3.7314958231708806E-5	4.1461064701898675E-6	2.7156997379743634E-4	0.1649922339279707	0.0011132295872459796
94-95	0.013586790902812196	4.146106470189867E-5	4.1461064701898675E-6	2.7571608026762616E-4	0.16594791146934945	0.0011505445454776883
96-97	0.013599229222222765	4.146106470189867E-5	4.1461064701898675E-6	2.7986218673781605E-4	0.16697199976748633	0.0011712750778286375
98-99	0.013611667541633335	4.146106470189867E-5	4.1461064701898675E-6	2.81935239972911E-4	0.16809559462090778	0.0012376127813516755
100-101	0.01362203280780881	4.146106470189867E-5	4.1461064701898675E-6	2.8608134644310087E-4	0.16932906129578928	0.0013039504848747134
102-103	0.013626178914278999	4.5607171172088546E-5	4.1461064701898675E-6	3.0473882555895527E-4	0.1702329125062907	0.0013516307092818968
104-105	0.01363861723368957	4.5607171172088546E-5	4.1461064701898675E-6	3.1717714496952486E-4	0.17124456248501702	0.0014262606257453144
106-107	0.013646909446629949	4.5607171172088546E-5	4.1461064701898675E-6	3.254693579099046E-4	0.17214426758904822	0.0014532103178015487
108-109	0.013682151351626563	4.5607171172088546E-5	4.1461064701898675E-6	3.3168851761518944E-4	0.17307299543837074	0.0015154019148543967
110-111	0.013723612416328461	4.5607171172088546E-5	4.1461064701898675E-6	3.5241904996613873E-4	0.1741509831206201	0.0015900318313178143
112-113	0.013754708214854885	4.5607171172088546E-5	4.1461064701898675E-6	3.6278431614161343E-4	0.1751688522590517	0.001658442588075947
114-115	0.013769219587500551	4.5607171172088546E-5	4.1461064701898675E-6	3.710765290819932E-4	0.1761473333860165	0.0017040497592480356
116-117	0.01378165790691112	4.5607171172088546E-5	4.1461064701898675E-6	3.7729568878727796E-4	0.17719215221650436	0.0017455108239499344
118-119	0.013787877066616404	4.5607171172088546E-5	4.1461064701898675E-6	3.7729568878727796E-4	0.17810014953347594	0.0017973371548273076
120-121	0.013800315386026975	4.5607171172088546E-5	4.1461064701898675E-6	3.7729568878727796E-4	0.17903716959573887	0.001847090432469586
122-123	0.013804461492497165	4.5607171172088546E-5	4.1461064701898675E-6	3.8558790172765766E-4	0.18017734887504108	0.001942450881283953
124-125	0.013806534545732259	4.975327764227841E-5	4.1461064701898675E-6	3.938801146680374E-4	0.18128228624934667	0.0019922041589262316
126-127	0.013812753705437544	4.975327764227841E-5	4.1461064701898675E-6	3.980262211382273E-4	0.18227320569572206	0.0020461035430386996
128-129	0.013823118971613018	4.975327764227841E-5	4.1461064701898675E-6	4.0839148731370195E-4	0.1833988736023786	0.002089637660975693
130-131	0.013827265078083208	5.1826330877373346E-5	4.1461064701898675E-6	4.104645405487969E-4	0.1845079570831544	0.002116587353031927
132-133	0.013829338131318304	5.389938411246828E-5	8.292212940379735E-6	4.1461064701898677E-4	0.1855776525524634	0.002170486737144396
134-135	0.013831411184553399	5.389938411246828E-5	8.292212940379735E-6	4.1461064701898677E-4	0.1865602797858984	0.002226459174491959
136-137	0.013831411184553399	5.8045490582658146E-5	8.292212940379735E-6	4.1875675348917665E-4	0.18766521716020396	0.002284504665074617
138-139	0.013833484237788494	6.219159705284801E-5	1.2438319410569603E-5	4.229028599593665E-4	0.1886354060742284	0.0023384040491870855
>>END_MODULE
