##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24152057
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.66465075831843	37.0	35.0	38.0	32.0	39.0
2	35.15409188542409	35.0	34.0	38.0	32.0	39.0
3	35.38522515908272	36.0	34.0	38.0	32.0	39.0
4	35.38014091304935	36.0	35.0	38.0	32.0	39.0
5	35.30285523920385	36.0	35.0	38.0	32.0	39.0
6	35.21222101289344	36.0	34.0	37.0	32.0	39.0
7	35.08665878852472	35.0	34.0	37.0	33.0	39.0
8	35.24582427906658	35.0	34.0	37.0	33.0	39.0
9	35.21118275764255	35.0	34.0	37.0	32.0	39.0
10-14	35.24915735334675	35.4	34.2	37.4	32.2	39.0
15-19	35.22701994285621	36.0	34.0	37.0	32.0	39.0
20-24	35.22742954771927	36.0	34.0	37.0	32.0	39.0
25-29	35.21698197383353	36.0	34.0	37.0	32.0	39.0
30-34	35.19014202392782	36.0	34.0	37.0	32.0	39.0
35-39	35.16291535747867	35.8	34.0	37.0	32.0	39.0
40-44	35.14011050901379	35.4	34.0	37.0	32.0	39.0
45-49	35.12683241845612	35.6	34.0	37.0	32.0	39.0
50-54	35.09893105171125	35.2	34.0	37.0	32.0	39.0
55-59	35.075702082021415	35.0	34.0	37.0	32.0	39.0
60-64	35.05517836431075	35.0	34.0	37.0	32.0	39.0
65-69	35.021470916535186	35.0	34.0	37.0	32.0	39.0
70-74	35.00901266504961	35.0	34.0	37.0	32.0	39.0
75-79	34.980095335150956	35.0	34.0	37.0	32.0	39.0
80-84	34.956976732872064	35.0	34.0	37.0	32.0	39.0
85-89	34.92800748193001	35.0	34.0	37.0	32.0	39.0
90-94	34.897568128462105	35.0	34.0	37.0	31.0	39.0
95-99	34.86229638328528	35.0	34.0	37.0	31.0	39.0
100-104	34.83273438779976	35.0	34.0	37.0	31.0	39.0
105-109	34.80652709622207	35.0	34.0	37.0	31.0	39.0
110-114	34.76245468450161	35.0	34.0	37.0	31.0	39.0
115-119	34.73608002829738	35.0	34.0	37.0	31.0	39.0
120-124	34.696766614951265	35.0	34.0	37.0	31.0	39.0
125-129	34.651323752672496	35.0	34.0	37.0	31.0	39.0
130-134	34.61437663052882	35.0	34.0	37.0	30.4	39.0
135-139	34.57136546174928	35.0	34.0	37.0	30.0	39.0
140-144	34.52083740113731	35.0	34.0	37.0	30.0	39.0
145-149	34.482104161976764	35.0	34.0	37.0	30.0	39.0
150	34.448020928403736	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	6.0
22	108.0
23	1144.0
24	8387.0
25	38678.0
26	114002.0
27	241904.0
28	388817.0
29	529587.0
30	687018.0
31	900232.0
32	1213416.0
33	1701638.0
34	2570182.0
35	4535187.0
36	1.0162337E7
37	1059396.0
38	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.09234203926592	12.359296828840003	14.516922804287727	37.031438327606345
2	22.41071531343577	12.830177929671956	34.15488353670862	30.604223220183652
3	21.078038759358687	16.85198959121056	27.534525653803744	34.535445995627015
4	24.40259550658843	24.03988275205249	22.298706677000155	29.258815064358927
5	24.599322761945107	30.7094992568086	24.309362389848825	20.38181559139747
6	18.989774734451974	37.733705863793965	25.321059095045783	17.955460306708282
7	15.02088803840002	26.703846962352095	39.89700006968407	18.378264929563812
8	17.347354047106467	26.593005059439463	31.816878889577353	24.242762003876717
9	18.15128339852492	24.712480058496457	33.060170446288055	24.076066096690568
10-14	19.108910307324205	30.829222001045814	27.484668387566362	22.57719930406362
15-19	19.26819904392164	29.19045463879555	28.412481436873094	23.128864880409715
20-24	19.34563139932645	29.31776164449192	28.089128727041658	23.247478229139976
25-29	19.470242253986164	29.38489832588656	27.93110792882637	23.213751491300904
30-34	19.38063009945954	29.321025357963325	27.89834231004714	23.40000223252999
35-39	19.471993996814025	29.13496425638128	28.037957508022306	23.35508423878239
40-44	19.474606835632663	29.19417509342095	27.99294406115887	23.33827400978752
45-49	19.565351559599428	29.030354476664428	27.9952433618398	23.409050601896343
50-54	19.563186594107336	29.097448882713923	27.989740479527867	23.349624043650866
55-59	19.644302350943075	29.033617039216182	27.94534951708026	23.376731092760487
60-64	19.667933143302903	28.94216202905468	27.877447358192626	23.51245746944979
65-69	19.644407516128943	28.86525862033772	28.038682548503257	23.451651315030084
70-74	19.73653267290367	28.843974080759228	28.004502775927627	23.414990470409478
75-79	19.624943713799546	28.84241007463552	28.01330565740605	23.51934055415888
80-84	19.811041759634985	28.720119911665947	27.901906809101973	23.566931519597095
85-89	19.775560035403764	28.757864646024807	28.031563983199607	23.435011335371822
90-94	19.900704694976838	28.647769363001423	27.95762057050738	23.49390537151436
95-99	19.941917794271014	28.637006220781924	27.983064058817845	23.438011926129217
100-104	19.994238002303426	28.550989245206704	27.963672402300823	23.491100350189047
105-109	19.92604513376682	28.50741726533275	28.028953096609527	23.537584504290905
110-114	19.99081452500145	28.553174571010643	27.93744367247023	23.518567231517675
115-119	20.06368858166308	28.489061967384128	28.004380386940365	23.44286906401243
120-124	20.085576427530004	28.33990465263186	28.100035736962308	23.47448318287583
125-129	20.169853189176532	28.352067573273537	28.02578615117882	23.45229308637111
130-134	20.08810440266134	28.461593370130245	27.993357742049653	23.45694448515876
135-139	20.157000083711452	28.321888172582355	28.014700522307614	23.506411221398587
140-144	20.244083365592815	28.245897544608678	28.075448211430466	23.434570878368042
145-149	20.241739641818487	28.50393824804932	27.981669187627585	23.272652922504605
150	20.2075238083566	28.3763422891597	27.802204072006553	23.613929830477147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.0
5	1.0
6	4.0
7	5.5
8	7.0
9	12.5
10	29.5
11	45.0
12	89.5
13	160.0
14	269.5
15	454.0
16	779.0
17	1454.0
18	2944.0
19	5465.5
20	6968.0
21	8869.5
22	13000.5
23	18925.5
24	27680.0
25	39363.5
26	53499.5
27	72450.5
28	100236.0
29	137124.0
30	181571.5
31	232865.0
32	287069.5
33	346246.5
34	416735.0
35	502799.5
36	600679.0
37	711668.5
38	838469.5
39	979455.0
40	1127930.0
41	1273594.5
42	1407182.0
43	1517289.0
44	1598221.0
45	1628288.5
46	1585094.0
47	1489605.5
48	1345525.5
49	1166517.5
50	982780.0
51	803047.5
52	639571.0
53	506982.5
54	405719.0
55	314668.5
56	234371.0
57	166541.0
58	112138.0
59	74427.0
60	52275.0
61	39531.0
62	29212.5
63	19932.5
64	12759.0
65	8537.0
66	5900.0
67	3943.0
68	2452.5
69	1636.0
70	1587.0
71	1970.0
72	2085.0
73	1487.0
74	880.0
75	529.0
76	264.0
77	109.5
78	33.5
79	12.5
80	4.5
81	2.5
82	3.0
83	6.0
84	5.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001353921945447545
2	6.417672830103042E-4
3	6.66609887513929E-4
4	6.748907556818038E-4
5	5.630990354154928E-4
6	4.844307878206813E-4
7	8.115250804517396E-4
8	7.121546624372408E-4
9	6.914524920175536E-4
10-14	8.040722991006521E-4
15-19	6.020191158045047E-4
20-24	5.8048885856803E-4
25-29	6.508762379949666E-4
30-34	3.5524924440183295E-4
35-39	3.378594212492957E-4
40-44	1.3911858522029823E-4
45-49	1.705858842582228E-4
50-54	2.111621382808098E-4
55-59	3.014236013106461E-4
60-64	2.699563022727215E-4
65-69	1.6230501609034792E-4
70-74	9.27457234801988E-5
75-79	1.854914469603976E-4
80-84	4.802903537367438E-5
85-89	1.9956892284578494E-4
90-94	7.7840160778024E-5
95-99	5.2997556274399314E-5
100-104	6.955929261014911E-5
105-109	1.2007258843418597E-4
110-114	5.216946945761183E-5
115-119	1.4657136657138562E-4
120-124	1.333219775027858E-4
125-129	9.522998393056127E-5
130-134	1.2007258843418597E-4
135-139	1.8383527332682263E-4
140-144	0.027740908362380894
145-149	9.605807074734876E-5
150	1.3663432476993575E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4152057E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.03599046260572
#Duplication Level	Percentage of total
1	38.27992359971105
2	11.295087599844198
3	6.637354870844152
4	4.401633062391659
5	3.371823099579327
6	2.6010408425135148
7	2.0881089809840674
8	1.7222497110290977
9	1.4947213240461579
>10	16.621822373739292
>50	4.582497739917303
>100	5.575077014360337
>500	0.859514925440049
>1k	0.46914485559984503
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.140434083937447E-6	1.1593215435024851E-4	1.2007258843418595E-4
2	0.0	0.0	0.0	8.280868167874894E-6	0.001473994533881731	3.353751607989332E-4
3	4.140434083937447E-6	0.0	0.0	8.280868167874894E-6	0.002596052170628779	4.347455788134319E-4
4	2.484260450362468E-5	0.0	0.0	8.280868167874894E-6	0.003999659325083574	5.051329582403685E-4
5	2.8983038587562128E-5	0.0	0.0	1.242130225181234E-5	0.0056268499200709905	5.548181672476179E-4
6	3.312347267149958E-5	0.0	0.0	1.242130225181234E-5	0.006753047990901976	6.086438103388047E-4
7	3.312347267149958E-5	0.0	0.0	1.242130225181234E-5	0.007841982154977525	6.707503215978664E-4
8	3.312347267149958E-5	0.0	0.0	1.656173633574979E-5	0.009725879663169063	7.452781351087404E-4
9	4.968520900724936E-5	0.0	0.0	1.656173633574979E-5	0.012938856512304522	8.363676849553642E-4
10-11	5.7966077175124256E-5	0.0	0.0	1.8631953377718512E-5	0.017592704422650214	9.667913585993939E-4
12-13	6.003629421709298E-5	2.0702170419687236E-6	0.0	2.277238746165596E-5	0.02106031796794782	0.0010661617766138925
14-15	6.21065112590617E-5	4.140434083937447E-6	0.0	3.105325562953085E-5	0.02507032837824124	0.001153110892376579
16-17	6.417672830103042E-5	4.140434083937447E-6	0.0	3.7263906755437024E-5	0.028581416481420194	0.001250411093349109
18-19	6.831716238496788E-5	4.140434083937447E-6	0.0	3.7263906755437024E-5	0.03165568878874375	0.0013394304261537641
20-21	8.073846463678021E-5	4.140434083937447E-6	0.0	4.554477492331192E-5	0.03486038476971133	0.0014056773714967632
22-23	8.487889872071766E-5	4.140434083937447E-6	0.0	4.968520900724936E-5	0.037725565155796045	0.0014946967043014182
24-25	8.694911576268638E-5	4.140434083937447E-6	0.0	4.968520900724936E-5	0.04048516447274035	0.0016375416801972602
26-27	8.694911576268638E-5	4.140434083937447E-6	0.0	5.382564309118681E-5	0.04402109518042294	0.0017369120982117588
28-29	9.108954984662383E-5	4.140434083937447E-6	0.0	6.21065112590617E-5	0.04744937460192314	0.0018093696946806642
30-31	9.108954984662383E-5	4.140434083937447E-6	0.0	6.21065112590617E-5	0.05109088637874613	0.0018921783763594133
32-33	9.522998393056127E-5	4.140434083937447E-6	0.0	8.69491157626864E-5	0.05443842733560955	0.0020143211818355678
34-35	9.522998393056127E-5	4.140434083937447E-6	0.0	9.108954984662383E-5	0.057709370261920134	0.002148885289563535
36-37	9.730020097252999E-5	4.140434083937447E-6	0.0	9.108954984662383E-5	0.06047311001294838	0.002223413103074409
38-39	1.0765128618237362E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.06329895627523568	0.002297940916585283
40-41	1.0765128618237362E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.06649744160507737	0.002403521985725688
42-43	1.1179172026631106E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.06941023698312736	0.0025111732719080614
44-45	1.159321543502485E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.07232096214413539	0.0025836308683769666
46-47	1.2007258843418595E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.07505778907361804	0.0026498778137199657
48-49	1.2007258843418595E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.07865375607551771	0.0027244056272308402
50-51	1.2007258843418595E-4	4.140434083937447E-6	0.0	9.522998393056127E-5	0.08151893646160242	0.0027803014873639957
52-53	1.242130225181234E-4	4.140434083937447E-6	0.0	9.937041801449873E-5	0.08427853577854673	0.0028465484327069948
54-55	1.324938906859983E-4	4.140434083937447E-6	0.0	1.0144063505646745E-4	0.08696981793310607	0.0029045145098821187
56-57	1.3663432476993575E-4	4.140434083937447E-6	0.0	1.1800237139221723E-4	0.0898929643963659	0.0029521295018474
58-59	1.428449758958419E-4	4.140434083937447E-6	0.0	1.242130225181234E-4	0.09231511833546932	0.0029893934086028364
60-61	1.4698540997977938E-4	4.140434083937447E-6	0.0	1.2628323956009212E-4	0.09498776853665095	0.0030556403539458354
62-63	1.594067122315917E-4	4.140434083937447E-6	0.0	1.2835345660206084E-4	0.09752378441306261	0.003175712942380022
64-65	1.6561736335749785E-4	4.140434083937447E-6	0.0	1.2835345660206084E-4	0.10018815374607637	0.003248170538848927
66-67	1.7389823152537276E-4	6.210651125906171E-6	0.0	1.2835345660206084E-4	0.10301400000836368	0.0033019961819401138
68-69	1.780386656093102E-4	8.280868167874894E-6	0.0	1.2835345660206084E-4	0.1055810691404049	0.003359962259115238
70-71	1.842493167352164E-4	8.280868167874894E-6	0.0	1.2835345660206084E-4	0.10798666134317256	0.0034241389874162687
72-73	1.9460040194506E-4	8.280868167874894E-6	0.0	1.2835345660206084E-4	0.11035705985622674	0.0034986668009271424
74-75	1.9874083602899745E-4	1.242130225181234E-5	0.0	1.2835345660206084E-4	0.11274402010561668	0.0036166691723193596
76-77	2.0081105307096617E-4	1.656173633574979E-5	0.0	1.2835345660206084E-4	0.11539182770229467	0.003684986334704328
78-79	2.028812701129349E-4	1.656173633574979E-5	0.0	1.2835345660206084E-4	0.11814935680219701	0.003720180024417796
80-81	2.111621382808098E-4	1.656173633574979E-5	0.0	1.2835345660206084E-4	0.12090274546801541	0.0037470928459633892
82-83	2.1323235532277852E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.12362508087820429	0.003800918489054576
84-85	2.1944300644868468E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.1262169926147491	0.0038816569536913566
86-87	2.2565365757459084E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.12920845624039393	0.003929271945656637
88-89	2.297940916585283E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.13185419362002995	0.003978957154663886
90-91	2.3186430870049702E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.1344730181781204	0.0040286423636711356
92-93	2.3393452574246574E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.13669229084711088	0.004109380828307916
94-95	2.3600474278443446E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.13869419072669464	0.00420047037815454
96-97	2.4014517686837193E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.1407271438619079	0.004256366238287696
98-99	2.5049626207821553E-4	1.656173633574979E-5	4.140434083937447E-6	1.2835345660206084E-4	0.14240194944886062	0.004306051447294944
100-101	2.5256647912018425E-4	2.0702170419687235E-5	4.140434083937447E-6	1.2835345660206084E-4	0.1443810769409827	0.004353666439260225
102-103	2.5463669616215297E-4	2.0702170419687235E-5	4.140434083937447E-6	1.2835345660206084E-4	0.1461407614266561	0.0044054218653094435
104-105	2.567069132041217E-4	2.0702170419687235E-5	4.140434083937447E-6	1.3042367364402956E-4	0.14786939265670002	0.0044509666402327555
106-107	2.6084734728805913E-4	2.0702170419687235E-5	4.140434083937447E-6	1.428449758958419E-4	0.1495462684606947	0.004494441198114098
108-109	2.6084734728805913E-4	2.0702170419687235E-5	4.140434083937447E-6	1.5319606110568554E-4	0.15170757505251004	0.004531705104869535
110-111	2.649877813719966E-4	2.0702170419687235E-5	6.210651125906171E-6	1.7182801448340404E-4	0.1540448500928927	0.00458967118204466
112-113	2.649877813719966E-4	2.0702170419687235E-5	8.280868167874894E-6	1.8010888265127895E-4	0.15587078152390912	0.004647637259219784
114-115	2.649877813719966E-4	2.0702170419687235E-5	8.280868167874894E-6	1.8217909969324767E-4	0.15739653148384009	0.004689041600059158
116-117	2.732686495398715E-4	2.484260450362468E-5	8.280868167874894E-6	1.8217909969324767E-4	0.15895126448235858	0.004738726809066408
118-119	2.732686495398715E-4	2.484260450362468E-5	8.280868167874894E-6	1.9045996786112255E-4	0.16060536789889157	0.0047718502817379075
120-121	2.753388665818402E-4	2.484260450362468E-5	8.280868167874894E-6	1.9460040194506E-4	0.16232364804372562	0.004821535490745156
122-123	2.7740908362380894E-4	2.484260450362468E-5	8.280868167874894E-6	1.9667061898702873E-4	0.16447667376737313	0.0048836420020042185
124-125	2.7947930066577766E-4	2.484260450362468E-5	8.280868167874894E-6	1.9874083602899745E-4	0.16608109197489887	0.004922976125801624
126-127	2.815495177077464E-4	2.484260450362468E-5	8.280868167874894E-6	2.1323235532277852E-4	0.16760684193482983	0.0049498889473472175
128-129	2.856899517916838E-4	2.484260450362468E-5	8.280868167874894E-6	2.1530257236474724E-4	0.1692754368706566	0.0049705911177669055
130-131	2.856899517916838E-4	2.484260450362468E-5	8.280868167874894E-6	2.1530257236474724E-4	0.1709109083338119	0.005003714590438404
132-133	2.856899517916838E-4	2.8983038587562128E-5	8.280868167874894E-6	2.1530257236474724E-4	0.17228346223263716	0.005032697629025966
134-135	2.8983038587562126E-4	2.8983038587562128E-5	8.280868167874894E-6	2.1944300644868468E-4	0.17382784414594582	0.005057540233529591
136-137	2.8983038587562126E-4	2.8983038587562128E-5	8.280868167874894E-6	2.1944300644868468E-4	0.1758069716380679	0.005076172186907309
138-139	2.8983038587562126E-4	2.8983038587562128E-5	8.280868167874894E-6	2.1944300644868468E-4	0.1773182300787051	0.005094804140285028
>>END_MODULE
