##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.2.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24104263
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.70865838129961	37.0	35.0	38.0	32.0	39.0
2	35.204510919914874	35.0	34.0	38.0	32.0	39.0
3	35.426403329568714	36.0	34.0	38.0	32.0	39.0
4	35.42206330888441	36.0	35.0	38.0	32.0	39.0
5	35.33902579805074	36.0	35.0	38.0	32.0	39.0
6	35.25047374400122	36.0	34.0	37.0	33.0	39.0
7	35.11512241631283	35.0	34.0	37.0	33.0	39.0
8	35.28881422344255	35.0	34.0	37.0	33.0	39.0
9	35.253927614380906	35.0	34.0	37.0	33.0	39.0
10-14	35.292584162394846	35.6	34.2	37.4	32.8	39.0
15-19	35.2664597627399	36.0	34.0	37.0	32.2	39.0
20-24	35.27161886675398	36.0	34.0	37.0	32.0	39.0
25-29	35.257418216852344	36.0	34.0	37.0	32.0	39.0
30-34	35.23427867510407	36.0	34.0	37.0	32.0	39.0
35-39	35.20220147780498	36.0	34.0	37.0	32.0	39.0
40-44	35.18484868838347	36.0	34.0	37.0	32.0	39.0
45-49	35.16742760398856	36.0	34.0	37.0	32.0	39.0
50-54	35.143329343859214	36.0	34.0	37.0	32.0	39.0
55-59	35.11692096124242	35.8	34.0	37.0	32.0	39.0
60-64	35.09833785003093	35.6	34.0	37.0	32.0	39.0
65-69	35.06214603615966	35.0	34.0	37.0	32.0	39.0
70-74	35.05033620816367	35.0	34.0	37.0	32.0	39.0
75-79	35.01887802170097	35.0	34.0	37.0	32.0	39.0
80-84	34.99818263682238	35.0	34.0	37.0	32.0	39.0
85-89	34.96789419365363	35.0	34.0	37.0	32.0	39.0
90-94	34.93861757150592	35.0	34.0	37.0	32.0	39.0
95-99	34.9019752149236	35.0	34.0	37.0	31.2	39.0
100-104	34.87343120177539	35.0	34.0	37.0	31.0	39.0
105-109	34.847910670407146	35.0	34.0	37.0	31.0	39.0
110-114	34.804075619320955	35.0	34.0	37.0	31.0	39.0
115-119	34.77782195622409	35.0	34.0	37.0	31.0	39.0
120-124	34.73925389048402	35.0	34.0	37.0	31.0	39.0
125-129	34.697424285488424	35.0	34.0	37.0	31.0	39.0
130-134	34.66147910848799	35.0	34.0	37.0	31.0	39.0
135-139	34.62010628576364	35.0	34.0	37.0	30.4	39.0
140-144	34.571146921189836	35.0	34.0	37.0	30.0	39.0
145-149	34.536559031072635	35.0	34.0	37.0	30.0	39.0
150	34.50671281673287	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	82.0
23	1129.0
24	8066.0
25	36500.0
26	107849.0
27	229404.0
28	373468.0
29	510929.0
30	664067.0
31	872284.0
32	1179700.0
33	1666921.0
34	2540869.0
35	4513426.0
36	1.0298641E7
37	1100905.0
38	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.00412131878053	12.290636362900912	15.069838182022977	36.63540413629559
2	22.619530001022646	12.78786031999957	34.02830100030721	30.564308678670578
3	21.29920793198938	16.95110009630305	27.547431005251095	34.20226096645648
4	24.529804802795873	24.192160789343383	22.255762871729004	29.02227153613174
5	24.549349506957345	30.99387527458502	24.217638318060484	20.239136900397156
6	18.9727145291638	37.844222407040576	25.157944813396632	18.02511825039899
7	14.953934777193773	26.629126416917565	40.02411790466711	18.39282090122155
8	17.40167134312119	26.482098243339397	31.743172848654254	24.37305756488516
9	18.06293687203955	24.68895030232709	32.99215243129924	24.255960394334124
10-14	19.115649006227102	30.850630531163997	27.443279012301097	22.590441450307804
15-19	19.322472380179764	29.150677112006246	28.40464950529166	23.12220100252233
20-24	19.355700776075516	29.30895466623773	28.061766394707387	23.27357816297937
25-29	19.533192675024537	29.35479047531783	27.865340939578946	23.246675910078682
30-34	19.390645896408685	29.31167375765219	27.822196322049113	23.475484023890004
35-39	19.49221253293879	29.116137911189593	28.01325861378643	23.378390942085186
40-44	19.464231869251737	29.210480595481314	27.926454290019137	23.39883324524781
45-49	19.639700612101926	29.00489693489698	27.946607591646355	23.408794861354735
50-54	19.605043707226137	29.11881305496953	27.908196139575043	23.367947098229294
55-59	19.68409649506667	29.01648493067348	27.868126362729544	23.43129221153031
60-64	19.67746446541793	28.95024658182937	27.80437617509559	23.567912777657106
65-69	19.666293683639175	28.81127650959575	27.992862296844013	23.529567509921062
70-74	19.75036340082603	28.812111795444434	27.96112119370876	23.47640361002078
75-79	19.600593360094408	28.824517430274295	27.984614575436805	23.590274634194493
80-84	19.84397357312507	28.66673802337762	27.82197164400469	23.66731675949262
85-89	19.7882980643083	28.72600049989607	27.968983377277524	23.51671805851811
90-94	19.97480327677049	28.6000017241776	27.883839914772857	23.541355084279054
95-99	20.00829247995482	28.619696291883614	27.90408303452294	23.467928193638627
100-104	20.039966402599774	28.475853590276166	27.897501543918864	23.586678463205196
105-109	19.970846617996937	28.482395376480714	27.95205954671204	23.59469845881031
110-114	20.03975996132267	28.519710599734598	27.853067132297436	23.58746230664529
115-119	20.126009402580866	28.453058846551972	27.92547682392549	23.49545492694167
120-124	20.134907619815973	28.287783606160577	28.054426203190797	23.522882570832657
125-129	20.239271952745767	28.333712575086796	27.917383290314064	23.50963218185337
130-134	20.144843396846365	28.432235988357235	27.878551657301788	23.544368957494616
135-139	20.229686996029468	28.289904090537405	27.89366545476505	23.58674345866808
140-144	20.3302589666575	28.190518949374365	27.982464153281178	23.496757930686957
145-149	20.28897537878854	28.377974524349664	27.98487522552256	23.348174871339236
150	20.254561128897294	28.288105448711697	27.761471741681852	23.695861680709154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.5
6	1.5
7	4.0
8	9.0
9	13.0
10	29.5
11	53.5
12	90.0
13	167.5
14	296.5
15	445.5
16	803.0
17	1520.5
18	3263.0
19	6221.0
20	7747.5
21	9645.0
22	13917.0
23	19871.5
24	29311.0
25	41636.5
26	55712.5
27	75039.5
28	103079.0
29	140198.0
30	184677.5
31	237160.0
32	291014.5
33	348893.5
34	419390.0
35	502896.0
36	597190.0
37	704236.5
38	825972.5
39	961581.5
40	1103052.0
41	1242129.0
42	1376966.5
43	1489375.5
44	1571669.5
45	1607513.5
46	1566445.0
47	1474652.0
48	1338231.0
49	1165764.5
50	985248.0
51	809657.5
52	654997.0
53	529569.0
54	436575.5
55	345999.5
56	258260.0
57	182187.5
58	118727.5
59	76611.0
60	53203.0
61	40409.5
62	30445.5
63	20464.0
64	12607.5
65	8422.0
66	5625.0
67	3762.0
68	2389.0
69	1605.5
70	1647.0
71	2120.5
72	2258.5
73	1593.0
74	935.0
75	570.0
76	283.0
77	111.0
78	36.5
79	10.0
80	8.0
81	7.0
82	6.5
83	6.5
84	6.0
85	3.5
86	1.0
87	0.5
88	0.5
89	1.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001360755149410708
2	6.969721496981675E-4
3	6.057019872376932E-4
4	7.384585871802013E-4
5	6.720802872089472E-4
6	4.8124267479159185E-4
7	7.426072309284047E-4
8	7.301612996837945E-4
9	9.832285683242007E-4
10-14	8.48812510882411E-4
15-19	5.874479547455983E-4
20-24	5.791506672491915E-4
25-29	7.334802146823572E-4
30-34	3.783563098361481E-4
35-39	3.4848607484908376E-4
40-44	1.5432954743316567E-4
45-49	1.7507276617418255E-4
50-54	2.348132361483112E-4
55-59	2.7795913112962635E-4
60-64	2.2319703365334173E-4
65-69	1.593079199310097E-4
70-74	1.0371609370508444E-4
75-79	1.941565274159181E-4
80-84	5.393236872664391E-5
85-89	2.1821866115549768E-4
90-94	7.301612996837945E-5
95-99	5.891074122448797E-5
100-104	7.052694371945743E-5
105-109	1.1201338120149122E-4
110-114	5.891074122448797E-5
115-119	1.2445931244610132E-4
120-124	1.460322599367589E-4
125-129	1.0537555120436579E-4
130-134	9.541880620867768E-5
135-139	2.1241055990801295E-4
140-144	0.030060242870732036
145-149	9.375934870939634E-5
150	1.3690524369071148E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4104263E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.129819336432774
#Duplication Level	Percentage of total
1	36.79833073017156
2	10.972303944199538
3	6.243738979999434
4	4.186951617539404
5	3.1127187349059877
6	2.49082804971192
7	2.080025364228895
8	1.6826103926520928
9	1.4342789426498626
>10	16.548013637062837
>50	4.65962094851973
>100	7.168940093495088
>500	1.492057629294267
>1k	1.108257366836766
>5k	0.021323568732643985
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.148643748203378E-6	1.3690524369071148E-4	1.5764846243172836E-4
2	0.0	4.148643748203378E-6	0.0	4.148643748203378E-6	0.0016553088555331479	3.6508064984189725E-4
3	0.0	4.148643748203378E-6	0.0	4.148643748203378E-6	0.0029911721424546356	4.729453872951851E-4
4	4.148643748203378E-6	4.148643748203378E-6	0.0	4.148643748203378E-6	0.0047170079417072405	5.60066906007456E-4
5	4.148643748203378E-6	4.148643748203378E-6	0.0	4.148643748203378E-6	0.006529965259672117	6.264452059787101E-4
6	1.2445931244610134E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.007766261096636724	6.637829997125405E-4
7	1.2445931244610134E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.009135313533543838	6.845262184535574E-4
8	1.2445931244610134E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.01085700068904824	7.592018059212181E-4
9	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.014428982956251349	8.753638308709127E-4
10-11	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.018917815491807405	9.894515339465056E-4
12-13	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.022531284196492543	0.0011097622026444037
14-15	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.02649946194164908	0.0012528904119574202
16-17	2.074321874101689E-5	4.148643748203378E-6	0.0	1.86688968669152E-5	0.030164788693186763	0.0013773497244035215
18-19	2.074321874101689E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.032996238051335565	0.0014893631056050126
20-21	2.9040506237423647E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.036134687046851424	0.0015889305555618937
22-23	3.73377937338304E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.03909474436119453	0.001680200718022368
24-25	3.73377937338304E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.041984274731818184	0.001800511386720266
26-27	3.73377937338304E-5	4.148643748203378E-6	0.0	2.4891862489220268E-5	0.04562678394274075	0.0019166734116699605
28-29	3.73377937338304E-5	4.148643748203378E-6	0.0	2.4891862489220268E-5	0.04936056331612379	0.0020100178960045364
30-31	4.5635081230237156E-5	4.148643748203378E-6	0.0	2.4891862489220268E-5	0.053222950645701136	0.002080544839723994
32-33	4.9783724978440536E-5	4.148643748203378E-6	0.0	4.978372497844054E-5	0.056463041413047976	0.00217388932405857
34-35	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.222965622305067E-5	0.0599375305521683	0.002304571602126977
36-37	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.222965622305067E-5	0.0629992296383424	0.0023709499020982306
38-39	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.06601944228703446	0.0024373282020694843
40-41	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.06918900611066184	0.0025389699739004675
42-43	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.07227974570307336	0.0026032739519976198
44-45	5.185804685254222E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.07547212706731585	0.002694544114458094
46-47	5.8081012474847295E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.07857116394722377	0.0027961858862890765
48-49	5.8081012474847295E-5	6.222965622305067E-6	4.148643748203378E-6	6.637829997125405E-5	0.08299984114843087	0.002868787151882636
50-51	7.260126559355911E-5	8.297287496406756E-6	4.148643748203378E-6	7.052694371945742E-5	0.08630631021574897	0.0029330911299797883
52-53	7.46755874676608E-5	8.297287496406756E-6	4.148643748203378E-6	7.052694371945742E-5	0.08937630658941947	0.002999469429951042
54-55	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.052694371945742E-5	0.09233843822563668	0.0030513274768035844
56-57	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.260126559355911E-5	0.09539806298993668	0.003113557133026635
58-59	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.674990934176249E-5	0.09783953983575436	0.003200678651738906
60-61	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10036191523466202	0.0032815772048288717
62-63	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10286769605857686	0.00338944194228216
64-65	8.089855308996587E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10565558465736953	0.003486935070364939
66-67	8.297287496406757E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10843517596866578	0.00354916472658799
68-69	8.297287496406757E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.11074804485828918	0.0036321376015520574
70-71	8.712151871227094E-5	8.297287496406756E-6	4.148643748203378E-6	8.297287496406757E-5	0.11318329873848455	0.003675698360908193
72-73	9.54188062086777E-5	8.297287496406756E-6	4.148643748203378E-6	8.919584058637262E-5	0.11548787034061153	0.003742076660879447
74-75	1.0371609370508444E-4	1.2445931244610134E-5	4.148643748203378E-6	9.127016246047431E-5	0.11799779980827457	0.0038478670764586333
76-77	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.1208603639945349	0.0039142453764298875
78-79	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.12368351606518731	0.003970252067030633
80-81	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.12659586397642608	0.0040055155388903614
82-83	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.12949369163454613	0.0040449276544982935
84-85	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.13233136395831724	0.004111305954469548
86-87	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.13578925852244478	0.004229542301293344
88-89	1.1201338120149122E-4	2.2817540615118578E-5	4.148643748203378E-6	9.127016246047431E-5	0.13860203898372664	0.004316663820005615
90-91	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.127016246047431E-5	0.141118191417012	0.004380967798102767
92-93	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.127016246047431E-5	0.14337297929416054	0.004447346098074021
94-95	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.127016246047431E-5	0.1452958756714528	0.004532393294912191
96-97	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.3344484334576E-5	0.14717935993313713	0.004582177019890631
98-99	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.956744995688107E-5	0.14887615522615233	0.004615366169876258
100-101	1.1616202494969459E-4	2.9040506237423647E-5	4.148643748203378E-6	9.956744995688107E-5	0.15086128125966763	0.004648555319861886
102-103	1.1616202494969459E-4	2.9040506237423647E-5	4.148643748203378E-6	9.956744995688107E-5	0.15269290747449943	0.004698339044840325
104-105	1.1616202494969459E-4	2.9040506237423647E-5	4.148643748203378E-6	9.956744995688107E-5	0.15444985810186357	0.004758494379189275
106-107	1.1823634682379627E-4	2.9040506237423647E-5	4.148643748203378E-6	1.0579041557918614E-4	0.15630222753543638	0.004820724035412325
108-109	1.2031066869789796E-4	2.9040506237423647E-5	4.148643748203378E-6	1.1201338120149122E-4	0.15840973855952367	0.004868433438516664
110-111	1.2031066869789796E-4	2.9040506237423647E-5	4.148643748203378E-6	1.1616202494969459E-4	0.16068526965541324	0.004943109025984325
112-113	1.2653363432020304E-4	2.9040506237423647E-5	4.148643748203378E-6	1.2031066869789796E-4	0.16228042317659744	0.005026081900948392
114-115	1.286079561943047E-4	2.9040506237423647E-5	4.148643748203378E-6	1.286079561943047E-4	0.16365984722287508	0.005063419694682223
116-117	1.327565999425081E-4	2.9040506237423647E-5	6.222965622305067E-6	1.286079561943047E-4	0.16522803455969592	0.005104906132164256
118-119	1.327565999425081E-4	2.9040506237423647E-5	8.297287496406756E-6	1.348309218166098E-4	0.16697461357768956	0.005144318247772188
120-121	1.3897956556481315E-4	2.9040506237423647E-5	8.297287496406756E-6	1.3690524369071148E-4	0.1687253412394314	0.005212770869617545
122-123	1.4105388743891484E-4	2.9040506237423647E-5	8.297287496406756E-6	1.3897956556481315E-4	0.17103198716343246	0.005264628916470087
124-125	1.4520253118711823E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4105388743891484E-4	0.1725462421315267	0.0052978180664557135
126-127	1.4520253118711823E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4105388743891484E-4	0.17422851717142318	0.005355899078930561
128-129	1.4935117493532159E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4105388743891484E-4	0.17605599474250677	0.00541605441327951
130-131	1.5349981868352497E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4520253118711823E-4	0.1777071549542917	0.005478284069502561
132-133	1.5972278430583005E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4520253118711823E-4	0.17914258569117006	0.005507324575739984
134-135	1.6179710617993175E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4520253118711823E-4	0.18074396217797656	0.00551562186323639
136-137	1.6179710617993175E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4935117493532159E-4	0.18302571623948843	0.005561256944466629
138-139	1.6387142805403344E-4	3.111482811152534E-5	8.297287496406756E-6	1.5557414055762667E-4	0.18466028187628056	0.0055861488069558485
>>END_MODULE
