##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24104263
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.09442719737998	37.0	35.0	38.0	33.0	39.0
2	35.50573004451536	36.0	35.0	38.0	33.0	39.0
3	35.57475800027572	36.0	35.0	37.0	33.0	39.0
4	35.71538889199807	36.0	35.0	38.0	33.0	39.0
5	35.60859657895369	36.0	35.0	38.0	33.0	39.0
6	35.575806155118705	36.0	35.0	37.0	33.0	39.0
7	35.48501482911965	35.0	34.0	37.0	33.0	39.0
8	35.66432667947574	36.0	35.0	38.0	33.0	39.0
9	35.64458286901367	36.0	35.0	38.0	33.0	39.0
10-14	35.613640981265426	36.0	35.0	37.8	33.0	39.0
15-19	35.56598656428533	36.0	35.0	38.0	33.0	39.0
20-24	35.53470595636963	36.0	35.0	38.0	33.0	39.0
25-29	35.48868144195074	36.0	35.0	38.0	33.0	39.0
30-34	35.45807584326474	36.0	35.0	38.0	33.0	39.0
35-39	35.4230190236474	36.0	34.8	38.0	32.8	39.0
40-44	35.382454904346176	36.0	34.0	38.0	32.0	39.0
45-49	35.33560198044636	36.0	34.0	38.0	32.0	39.0
50-54	35.292022046058825	36.0	34.0	38.0	32.0	39.0
55-59	35.25662439876299	36.0	34.0	38.0	32.0	39.0
60-64	35.197923545723015	36.0	34.0	38.0	32.0	39.0
65-69	35.143202129847324	36.0	34.0	38.0	32.0	39.0
70-74	35.08073707128071	36.0	34.0	38.0	31.8	39.0
75-79	35.01798564843073	35.8	34.0	37.8	31.0	39.0
80-84	34.95270405073161	35.2	34.0	37.4	31.0	39.0
85-89	34.86433769827354	35.0	34.0	37.0	31.0	39.0
90-94	34.790187038699344	35.0	34.0	37.0	31.0	39.0
95-99	34.72645908319205	35.0	34.0	37.0	30.2	39.0
100-104	34.66557924629349	35.0	34.0	37.0	30.0	39.0
105-109	34.54529335329605	35.0	34.0	37.0	30.0	39.0
110-114	34.46956339631707	35.0	34.0	37.0	30.0	39.0
115-119	34.37591601950244	35.0	34.0	37.0	29.2	39.0
120-124	34.309805663836315	35.0	34.0	37.0	29.0	39.0
125-129	34.166160931782066	35.0	34.0	37.0	28.8	39.0
130-134	34.070016536079116	35.0	34.0	37.0	28.0	39.0
135-139	33.95137806951409	35.0	33.2	37.0	28.0	39.0
140-144	33.80174075432217	35.0	33.0	37.0	27.0	39.0
145-149	33.686378969562355	35.0	33.0	37.0	27.0	39.0
150	33.57923617079684	35.0	33.0	37.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	60.0
21	776.0
22	4976.0
23	20758.0
24	58843.0
25	121381.0
26	210981.0
27	325589.0
28	442632.0
29	549334.0
30	674566.0
31	847788.0
32	1097530.0
33	1505592.0
34	2269423.0
35	3996522.0
36	1.0509211E7
37	1468298.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.24981713815519	20.12390920228509	17.246526060556178	28.379747599003547
2	24.00521849599799	25.4083437440091	33.21150287814235	17.374934881850567
3	20.551775426612295	28.720629209862174	31.865657954362682	18.861937409162852
4	23.90989925723927	32.933477368712744	24.06089329509888	19.095730078949106
5	25.087198613346896	35.11250811498905	23.45433839455512	16.345954877108937
6	19.839644132658194	39.015156779529	23.75439979226911	17.390799295543697
7	20.43234427038902	21.589064971619333	38.98764297419091	18.99094778380073
8	22.1718539994357	23.792326693415188	30.212792649997223	23.823026657151892
9	23.353503900948976	23.190918552456882	29.95955113831939	23.496026408274755
10-14	22.80904087380726	29.638032077562382	26.816418324011813	20.736508724618545
15-19	22.802584754406304	28.389184933801957	28.407921868426346	20.400308443365393
20-24	22.735928495303924	28.578380513023777	28.15374276326142	20.531948228410883
25-29	22.838953924501574	28.523179376421876	28.072735948771133	20.565130750305418
30-34	22.796102913414114	28.345482290829633	28.307060041620026	20.551354754136227
35-39	22.90069188176382	28.39380320402246	28.105936281893374	20.59956863232035
40-44	23.117319952906257	28.099075254862594	28.1603731256998	20.623231666531353
45-49	23.14291127673142	28.043841871456515	28.17198932819477	20.641257523617295
50-54	23.17330838947451	28.042975634641888	28.13695818038494	20.64675779549866
55-59	23.300704941694338	27.84507537110759	28.17660510922902	20.67761457796905
60-64	23.141194567948418	27.791032648457247	28.2848357570609	20.78293702653344
65-69	23.143091327870096	27.866238432595924	28.322093067106014	20.668577172427966
70-74	23.370768896771494	27.78123521138149	28.220862011006105	20.627133880840912
75-79	23.22771370358845	27.78238355597099	28.31621609837231	20.67368664206825
80-84	23.38551815502511	27.85934753533016	28.09606416923015	20.65907014041458
85-89	23.375110037589618	27.813020460322726	28.221238707858443	20.59063079422922
90-94	23.343231029299673	27.828751287687158	28.22031439003134	20.607703292981828
95-99	23.255571846357633	27.943180009278855	28.277395579362867	20.523852565000638
100-104	23.483621963467623	27.876537855565214	28.11240650668307	20.527433674284087
105-109	23.24581174707561	27.957074646920336	28.371326681923442	20.425786924080608
110-114	23.354166854219933	27.9332688993644	28.245048604058127	20.467515642357537
115-119	23.42100980229099	27.918240852250907	28.332735168048906	20.328014177409198
120-124	23.481709438699703	27.925020565864223	28.363980263574124	20.22928973186195
125-129	23.41518925511226	28.157349594136104	28.18556037162389	20.241900779127743
130-134	23.583537899499355	28.108534992337248	28.14656063120453	20.161366476958868
135-139	23.37261006486695	28.13921006421146	28.36592514776328	20.122254723158306
140-144	23.350650463778962	28.210293755921928	28.22312800022137	20.215927780077738
145-149	23.505102811067072	28.382059223300043	28.022185951090893	20.090652014541995
150	23.18228937346062	28.492727614198365	28.089591455254205	20.23539155708681
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	1.0
7	2.5
8	10.5
9	18.0
10	27.5
11	47.5
12	72.0
13	101.5
14	171.0
15	301.5
16	454.0
17	706.0
18	1234.5
19	2080.0
20	3188.5
21	5006.0
22	7521.0
23	11126.5
24	16357.5
25	24201.0
26	42266.5
27	58864.5
28	72435.0
29	98762.0
30	131629.0
31	165589.5
32	212273.0
33	274975.5
34	348719.5
35	440885.5
36	558937.0
37	696419.0
38	839287.0
39	1003422.0
40	1187907.5
41	1359201.5
42	1511252.5
43	1609253.5
44	1642312.5
45	1655328.5
46	1614110.5
47	1490156.5
48	1327712.0
49	1139730.0
50	947295.5
51	780361.0
52	631751.0
53	529841.0
54	448261.5
55	353532.5
56	262562.0
57	184194.0
58	126096.5
59	81380.0
60	55801.5
61	43455.0
62	32253.5
63	21511.5
64	14564.0
65	9935.5
66	6586.5
67	4633.0
68	3538.0
69	2745.0
70	2042.5
71	1648.0
72	1364.5
73	1107.5
74	1163.5
75	1152.0
76	755.5
77	357.5
78	187.5
79	87.5
80	20.0
81	4.5
82	4.0
83	3.0
84	3.0
85	3.0
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.1371790541780929
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0532976262331688
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4104263E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.35225742020604
#Duplication Level	Percentage of total
1	27.606543090588858
2	10.667320354511952
3	6.734799982378157
4	4.607792294255017
5	3.362414325482731
6	2.712190660882241
7	2.2461846428497076
8	1.8713827365067974
9	1.5383849975441855
>10	18.958891493946638
>50	5.80666519877081
>100	9.468762107246093
>500	2.1425151564767932
>1k	2.1196471680427464
>5k	0.11085914905709668
>10k+	0.04564664146020414
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2445931244610134E-5	0.0	0.0	0.0	0.0014893631056050126	0.0
2	3.3189149985627024E-5	0.0	0.0	8.297287496406756E-6	0.010579041557918613	0.0
3	2.1572947490657566E-4	0.0	0.0	8.297287496406756E-6	0.01274878223822898	4.148643748203378E-6
4	3.4848607484908376E-4	0.0	0.0	1.2445931244610134E-5	0.015607197780741109	4.148643748203378E-6
5	3.775265810865074E-4	0.0	0.0	1.2445931244610134E-5	0.019166734116699606	4.148643748203378E-6
6	4.8124267479159185E-4	0.0	0.0	1.2445931244610134E-5	0.021411150384477634	8.297287496406756E-6
7	5.019858935326087E-4	0.0	0.0	1.6594574992813512E-5	0.024016498658349355	8.297287496406756E-6
8	5.517696185110493E-4	0.0	0.0	1.6594574992813512E-5	0.02937654638102812	8.297287496406756E-6
9	8.006882434032519E-4	0.0	0.0	2.074321874101689E-5	0.04098030294475297	8.297287496406756E-6
10-11	0.0010786473745328783	0.0	0.0	2.489186248922027E-5	0.05042883908128616	8.297287496406756E-6
12-13	0.001120133812014912	0.0	0.0	3.111482811152534E-5	0.057043851537796446	1.2445931244610134E-5
14-15	0.0012031066869789796	0.0	2.074321874101689E-6	3.3189149985627024E-5	0.07821230626300418	1.6594574992813512E-5
16-17	0.0012445931244610135	0.0	4.148643748203378E-6	3.3189149985627024E-5	0.09646219011135085	1.6594574992813512E-5
18-19	0.0015142549680942329	0.0	4.148643748203378E-6	3.73377937338304E-5	0.10273493945863435	1.6594574992813512E-5
20-21	0.0018295518929576896	0.0	4.148643748203378E-6	4.148643748203378E-5	0.11021079549289683	2.074321874101689E-5
22-23	0.0021552204271916547	0.0	4.148643748203378E-6	4.148643748203378E-5	0.11776132711462699	2.6966184363321958E-5
24-25	0.002182186611554977	0.0	4.148643748203378E-6	4.148643748203378E-5	0.12238291625012554	2.9040506237423647E-5
26-27	0.0022195244052888074	0.0	4.148643748203378E-6	6.015533434894898E-5	0.1266373504139081	3.73377937338304E-5
28-29	0.0024435511676917897	0.0	4.148643748203378E-6	6.222965622305067E-5	0.13138962182747507	4.148643748203378E-5
30-31	0.002733956230066026	6.222965622305067E-6	4.148643748203378E-6	6.222965622305067E-5	0.13465253013543704	4.9783724978440536E-5
32-33	0.0027650710581775513	1.2445931244610134E-5	4.148643748203378E-6	6.845262184535574E-5	0.13822036375889193	5.3932368726643915E-5
34-35	0.0028750101175049406	1.2445931244610134E-5	4.148643748203378E-6	7.052694371945742E-5	0.14152683282621004	5.600669060074561E-5
36-37	0.0029372397737279917	1.2445931244610134E-5	4.148643748203378E-6	7.46755874676608E-5	0.14366960732215708	6.222965622305067E-5
38-39	0.003040955867433076	1.2445931244610134E-5	4.148643748203378E-6	7.674990934176249E-5	0.14566717928691703	6.845262184535574E-5
40-41	0.003090739592411517	1.2445931244610134E-5	4.148643748203378E-6	1.0371609370508444E-4	0.14782447403598276	7.260126559355911E-5
42-43	0.003281577204828872	1.2445931244610134E-5	4.148643748203378E-6	1.0786473745328783E-4	0.14978263388513474	7.882423121586418E-5
44-45	0.003615543026559244	1.2445931244610134E-5	4.148643748203378E-6	1.0993905932738952E-4	0.15209757709663224	9.3344484334576E-5
46-47	0.004034556045127785	1.2445931244610134E-5	4.148643748203378E-6	1.2031066869789796E-4	0.15421338540821594	9.956744995688107E-5
48-49	0.004227467979419242	1.2445931244610134E-5	4.148643748203378E-6	1.2238499057199966E-4	0.15607197780741106	1.2031066869789796E-4
50-51	0.004694190401092122	1.4520253118711822E-5	4.148643748203378E-6	1.286079561943047E-4	0.1578310027566493	1.348309218166098E-4
52-53	0.004997041394710969	1.6594574992813512E-5	4.148643748203378E-6	1.327565999425081E-4	0.15973937888082285	1.638714280540334E-4
54-55	0.005279149169588798	1.6594574992813512E-5	4.148643748203378E-6	1.4105388743891484E-4	0.16128889732077684	1.9706057803966045E-4
56-57	0.005461689494509747	1.6594574992813512E-5	4.148643748203378E-6	1.4105388743891484E-4	0.16264757814831343	2.4062133739579593E-4
58-59	0.0055384394038515096	1.6594574992813512E-5	4.148643748203378E-6	1.4312820931301653E-4	0.16389424559464855	2.883307405001348E-4
60-61	0.005743797269387577	2.074321874101689E-5	4.148643748203378E-6	1.493511749353216E-4	0.16515958193785057	3.235942123598635E-4
62-63	0.00632460739413605	2.074321874101689E-5	4.148643748203378E-6	1.493511749353216E-4	0.16648714793727565	3.816752248347108E-4
64-65	0.007121146993791098	2.074321874101689E-5	4.148643748203378E-6	1.5349981868352497E-4	0.1675222345524524	4.169386966944395E-4
66-67	0.0076770652560503505	2.074321874101689E-5	4.148643748203378E-6	1.5557414055762667E-4	0.1685158347301471	4.3560759356135465E-4
68-69	0.008185274115205265	2.074321874101689E-5	4.148643748203378E-6	1.6594574992813513E-4	0.16956129295469435	4.729453872951851E-4
70-71	0.008490199430698214	2.2817540615118578E-5	4.148643748203378E-6	1.7631735929864357E-4	0.17067105515733877	5.331007216441341E-4
72-73	0.009270144455360448	2.4891862489220268E-5	4.148643748203378E-6	1.9291193429145706E-4	0.1715713108506989	6.036276653635915E-4
74-75	0.0097036777270477	2.4891862489220268E-5	4.148643748203378E-6	2.0328354366196553E-4	0.17250060705029646	6.409654590974218E-4
76-77	0.01003556922690397	2.4891862489220268E-5	4.148643748203378E-6	2.0328354366196553E-4	0.17359999764357034	6.845262184535573E-4
78-79	0.010164177183098276	2.4891862489220268E-5	4.148643748203378E-6	2.0328354366196553E-4	0.17462886129312477	7.363842653060997E-4
80-81	0.010359163439263835	2.9040506237423647E-5	4.148643748203378E-6	2.1158083115837227E-4	0.17570336002390946	7.716477371658283E-4
82-83	0.010518886223569664	3.3189149985627024E-5	4.148643748203378E-6	2.1572947490657566E-4	0.17666999401724084	8.193571402701672E-4
84-85	0.010734615698476242	3.3189149985627024E-5	4.148643748203378E-6	2.1572947490657566E-4	0.17774449274802553	8.566949340039975E-4
86-87	0.01079684535469929	3.526347185972872E-5	4.148643748203378E-6	2.240267624029824E-4	0.17857214717579212	8.898840839896246E-4
88-89	0.01081966289531441	4.148643748203378E-5	4.148643748203378E-6	2.281754061511858E-4	0.17949936905351555	9.666339933313871E-4
90-91	0.010825885860936715	4.148643748203378E-5	4.148643748203378E-6	2.3024972802528749E-4	0.1805137124499513	0.0010018974651911157
92-93	0.010846629079677731	4.5635081230237156E-5	4.148643748203378E-6	2.3439837177349087E-4	0.18157161660574314	0.0010267893276803362
94-95	0.01085700068904824	4.5635081230237156E-5	4.148643748203378E-6	2.3854701552169423E-4	0.18245735204598457	0.001047532546421353
96-97	0.01085700068904824	4.5635081230237156E-5	4.148643748203378E-6	2.447699811439993E-4	0.1834799927299167	0.0010848703401551835
98-99	0.01085700068904824	4.5635081230237156E-5	4.148643748203378E-6	2.5099294676630436E-4	0.1847079912793849	0.001120133812014912
100-101	0.010865297976544647	4.5635081230237156E-5	4.148643748203378E-6	2.655131998850162E-4	0.18591732093198618	0.0011574716057487426
102-103	0.010865297976544647	4.5635081230237156E-5	4.148643748203378E-6	2.821077748778297E-4	0.18688602924719167	0.0012010323651048779
104-105	0.010877743907789257	4.5635081230237156E-5	4.148643748203378E-6	2.883307405001348E-4	0.18784229163115254	0.0012653363432020303
106-107	0.010890189839033866	4.5635081230237156E-5	4.148643748203378E-6	2.9455370612243984E-4	0.18872387842764576	0.0012923025275653522
108-109	0.010921304667145392	4.5635081230237156E-5	4.148643748203378E-6	2.987023498706432E-4	0.18975274207720022	0.0013711267587812165
110-111	0.010948270851508714	4.5635081230237156E-5	4.148643748203378E-6	3.111482811152534E-4	0.19090606503920074	0.0014167618400114535
112-113	0.010983534323368443	4.5635081230237156E-5	4.148643748203378E-6	3.194455686116601E-4	0.19195774622937029	0.0014789914962345043
114-115	0.011006351863983563	4.9783724978440536E-5	4.148643748203378E-6	3.256685342339652E-4	0.1929160829352053	0.0015246265774647412
116-117	0.011020872117102273	4.9783724978440536E-5	4.148643748203378E-6	3.3189149985627026E-4	0.19396983844724894	0.0015702616586949786
118-119	0.011033318048346884	5.3932368726643915E-5	4.148643748203378E-6	3.360401436044736E-4	0.19486179685311267	0.0016636061430295545
120-121	0.011043689657717392	5.3932368726643915E-5	4.148643748203378E-6	3.4433743110088037E-4	0.19584917406518507	0.0017237614773785034
122-123	0.011043689657717392	5.3932368726643915E-5	4.148643748203378E-6	3.4433743110088037E-4	0.19708754422402378	0.0018025857085943679
124-125	0.011049912623339698	5.8081012474847295E-5	4.148643748203378E-6	3.464117529749821E-4	0.19819938074854226	0.0018689640085656216
126-127	0.0110519869452138	5.8081012474847295E-5	4.148643748203378E-6	3.567833623454905E-4	0.19930914295118668	0.0019457139179073842
128-129	0.0110519869452138	5.8081012474847295E-5	4.148643748203378E-6	3.6508064984189725E-4	0.2005682563287664	0.002016240861626842
130-131	0.011064432876458408	5.8081012474847295E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20169253878452953	0.0020701732303534852
132-133	0.011068581520206613	6.015533434894898E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20272969972158036	0.002101288058465011
134-135	0.011072730163954816	6.637829997125405E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20366107024305202	0.0021489974615693497
136-137	0.011072730163954816	7.052694371945742E-5	4.148643748203378E-6	3.6508064984189725E-4	0.2048621026081569	0.002178037967806774
138-139	0.011074804485828918	7.260126559355911E-5	4.148643748203378E-6	3.6508064984189725E-4	0.2058287366014883	0.0022236730490370104
>>END_MODULE
