##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24019105
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00272732893253	37.0	35.0	38.0	33.0	39.0
2	35.42800603935908	36.0	34.0	38.0	32.0	39.0
3	35.65489825703331	36.0	35.0	38.0	33.0	39.0
4	35.63656443485301	36.0	35.0	38.0	33.0	39.0
5	35.515885541946716	36.0	35.0	38.0	33.0	39.0
6	35.39614240414037	36.0	35.0	37.0	33.0	39.0
7	35.25063469267485	35.0	34.0	37.0	33.0	39.0
8	35.43797102348318	36.0	35.0	38.0	33.0	39.0
9	35.40236261925663	35.0	34.0	38.0	33.0	39.0
10-14	35.43321899796017	36.0	35.0	37.8	33.0	39.0
15-19	35.41690274471093	36.0	35.0	38.0	33.0	39.0
20-24	35.4078194836985	36.0	34.8	38.0	33.0	39.0
25-29	35.39282980777177	36.0	34.4	38.0	33.0	39.0
30-34	35.356495981011776	36.0	34.0	38.0	32.4	39.0
35-39	35.31482040650557	36.0	34.0	38.0	32.0	39.0
40-44	35.28369739005679	36.0	34.0	38.0	32.0	39.0
45-49	35.2555056901579	36.0	34.0	38.0	32.0	39.0
50-54	35.21426248813184	36.0	34.0	37.8	32.0	39.0
55-59	35.16803726033922	36.0	34.0	37.6	32.0	39.0
60-64	35.13484813859634	36.0	34.0	37.0	32.0	39.0
65-69	35.074157467565925	36.0	34.0	37.0	32.0	39.0
70-74	35.042876943166696	36.0	34.0	37.0	32.0	39.0
75-79	34.987346289547425	35.8	34.0	37.0	31.8	39.0
80-84	34.94738802299253	35.4	34.0	37.0	31.0	39.0
85-89	34.886524797655866	35.0	34.0	37.0	31.0	39.0
90-94	34.83543902239488	35.0	34.0	37.0	31.0	39.0
95-99	34.768117105112786	35.0	34.0	37.0	31.0	39.0
100-104	34.71555826080946	35.0	34.0	37.0	31.0	39.0
105-109	34.65746603797269	35.0	34.0	37.0	30.2	39.0
110-114	34.594137641681485	35.0	34.0	37.0	30.0	39.0
115-119	34.534807953918346	35.0	34.0	37.0	30.0	39.0
120-124	34.47067549769236	35.0	34.0	37.0	30.0	39.0
125-129	34.392741736213736	35.0	34.0	37.0	30.0	39.0
130-134	34.33221358581013	35.0	34.0	37.0	29.8	39.0
135-139	34.26870529938564	35.0	34.0	37.0	29.0	39.0
140-144	34.19741051966757	35.0	34.0	37.0	29.0	39.0
145-149	34.13476198218044	35.0	34.0	37.0	29.0	39.0
150	34.09185317271397	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	10.0
22	141.0
23	1378.0
24	8531.0
25	36074.0
26	104534.0
27	224899.0
28	367639.0
29	510379.0
30	677954.0
31	911719.0
32	1250082.0
33	1783858.0
34	2709010.0
35	4672665.0
36	9626422.0
37	1133788.0
38	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.581622643884934	12.239408036446154	14.874908696429825	36.30406062323909
2	22.461555614638456	12.480837031311319	34.04377004820604	31.013837305844188
3	21.312818336763815	16.19545740947722	27.418097902733763	35.0736263510252
4	24.547635931128983	23.268869451625818	22.408497997912313	29.77499661933289
5	24.658120255937682	30.28172063317281	24.37142189099517	20.68873721989434
6	19.258336502351515	37.589852802401566	25.200743111517603	17.95106758372932
7	15.13214481170361	26.59802909498618	39.67727101744604	18.592555075864166
8	17.3167862430048	26.844981586634496	31.634201072602373	24.20403109775833
9	17.899722256327298	24.99730941151407	33.00104097151124	24.101927360647394
10-14	19.062587196819443	30.81781967212705	27.55415747357356	22.56543565747994
15-19	19.310275876310016	29.130870188641556	28.42856999993813	23.130283935110295
20-24	19.354090752930343	29.278247329044	28.05954966238747	23.30811225563819
25-29	19.54425301265364	29.261328382462825	27.866340984808613	23.328077620074925
30-34	19.389546182907743	29.259266074846696	27.786365602987235	23.56482213925833
35-39	19.478334880482333	29.040744055058838	28.028488691515292	23.452432372943537
40-44	19.438864617985317	29.135307274057915	27.922499102629565	23.5033290053272
45-49	19.67981479909119	28.8996521737523	27.941613905029612	23.478919122126896
50-54	19.60080569747329	29.051368172488424	27.89164156526121	23.45618456477707
55-59	19.710266449516663	28.919034795487615	27.845408770394346	23.52528998460138
60-64	19.687209753798655	28.83594779579984	27.788258487278444	23.68858396312307
65-69	19.680446974320255	28.675682433596428	27.985288284902314	23.658582307181003
70-74	19.75041157074879	28.684130767349764	27.942755938069265	23.622701723832183
75-79	19.578733396248925	28.714090747696357	27.96194309082965	23.745232765225072
80-84	19.870948659266126	28.526380835098852	27.77480787179472	23.8278626338403
85-89	19.80516489658767	28.56613146864308	27.964983875014127	23.663719759755125
90-94	20.019050014919802	28.42293501158962	27.870825807513565	23.68718916597702
95-99	20.05137830164999	28.487322729017738	27.863189981934784	23.59810898739748
100-104	20.08175762575013	28.29126002627376	27.87161470145117	23.755367646524935
105-109	19.991054111459547	28.32520478116925	27.944156858706474	23.739584248664727
110-114	20.088909938070014	28.363140218929672	27.804982571322988	23.74296727167732
115-119	20.176688903937297	28.273413761965553	27.894680606098948	23.655216727998198
120-124	20.175421928450493	28.077571388643634	28.05249631538643	23.694510367519438
125-129	20.29000072958651	28.145936320536848	27.883340132648854	23.680722817227785
130-134	20.167270206367462	28.250952955011943	27.85102918108777	23.73074765753282
135-139	20.26938706401822	28.086041902439497	27.85439253538142	23.790178498160866
140-144	20.353556248824876	27.989156903208556	27.965838290107182	23.69144855785938
145-149	20.334804735374036	28.191394853529566	27.97730927888566	23.49649113221074
150	20.32056435807254	28.119939829494694	27.70417864100273	23.855317171430027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	3.0
8	7.0
9	11.5
10	21.0
11	42.5
12	67.5
13	119.5
14	233.5
15	403.5
16	733.0
17	1485.5
18	3447.5
19	6954.5
20	8305.0
21	9442.0
22	13541.5
23	19660.5
24	29661.5
25	41844.0
26	55621.5
27	75525.0
28	103864.5
29	141045.5
30	186323.0
31	240158.0
32	294572.5
33	351736.0
34	419056.0
35	499092.0
36	590448.5
37	692937.0
38	806392.5
39	930922.0
40	1063040.5
41	1197127.0
42	1328235.0
43	1438916.0
44	1530705.0
45	1581362.0
46	1551938.5
47	1472909.5
48	1344954.5
49	1178289.5
50	1003442.5
51	834812.0
52	686708.0
53	561902.0
54	466931.5
55	373277.5
56	280616.5
57	197053.0
58	127331.0
59	80072.5
60	54285.5
61	41282.0
62	30847.5
63	20944.5
64	13240.0
65	8846.0
66	5987.5
67	4156.0
68	2754.0
69	1900.0
70	1979.5
71	2556.0
72	2703.0
73	1927.0
74	1145.0
75	671.5
76	355.0
77	137.5
78	35.0
79	11.0
80	6.5
81	7.5
82	5.5
83	4.0
84	2.0
85	1.0
86	1.5
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	1.0
96	1.5
97	0.5
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013697429608638624
2	5.120923531497114E-4
3	5.120923531497114E-4
4	7.369133862398286E-4
5	5.537258777960294E-4
6	4.121718939985482E-4
7	7.910369682800421E-4
8	5.787059925838203E-4
9	8.160170830678328E-4
10-14	6.952798615935107E-4
15-19	6.045187778645374E-4
20-24	5.029329777275214E-4
25-29	6.827898041996153E-4
30-34	3.7053836935223023E-4
35-39	3.613789939300403E-4
40-44	1.6070540513478748E-4
45-49	1.815221674579465E-4
50-54	2.697852397081407E-4
55-59	2.955980249888578E-4
60-64	2.3647841999108627E-4
65-69	1.7819148548624107E-4
70-74	1.1157784605213225E-4
75-79	1.856855199225783E-4
80-84	5.4956252533139765E-5
85-89	1.8818353140135738E-4
90-94	6.744630992703517E-5
95-99	6.66136394341088E-5
100-104	5.079290006850796E-5
105-109	1.0991250506627953E-4
110-114	5.329091154728705E-5
115-119	1.2573324443188036E-4
120-124	1.357252903469967E-4
125-129	8.576506077141508E-5
130-134	1.1574119851676404E-4
135-139	1.8818353140135738E-4
140-144	0.02360620847446231
145-149	7.577301485629878E-5
150	1.165738690096904E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4019105E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.58740411277164
#Duplication Level	Percentage of total
1	33.62177584066462
2	10.342039509504666
3	5.948982421577634
4	4.005720994508885
5	3.1360438814633183
6	2.59699329069135
7	2.0264862045168774
8	1.6696639113605138
9	1.4885853863918517
>10	17.771114474195183
>50	5.234412128859205
>100	8.354734657176895
>500	2.036631372779201
>1k	1.7118968899241471
>5k	0.054919036385601916
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	8.3267049292636E-6	7.077699189874061E-5	1.2906392640358582E-4
2	0.0	4.1633524646318E-6	0.0	1.2490057393895401E-5	0.001269822501712699	3.7053836935223023E-4
3	0.0	4.1633524646318E-6	0.0	1.2490057393895401E-5	0.0024896847738498165	4.912755908265524E-4
4	8.3267049292636E-6	4.1633524646318E-6	0.0	1.2490057393895401E-5	0.004050941948086742	5.787059925838203E-4
5	1.2490057393895401E-5	4.1633524646318E-6	0.0	2.0816762323159003E-5	0.0057620798110504115	6.245028696947701E-4
6	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.007002758845510688	6.744630992703516E-4
7	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.008243437879970965	7.119332714520379E-4
8	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.009996209267580953	7.660568534922513E-4
9	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.013147867083307226	8.451605503202555E-4
10-11	3.12251434847385E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.017590164163069355	9.700611242592094E-4
12-13	3.33068197170544E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.021079053528430806	0.0010845533170365839
14-15	3.33068197170544E-5	4.1633524646318E-6	0.0	2.7061791020106703E-5	0.024967624730396907	0.0011719837187938518
16-17	3.33068197170544E-5	4.1633524646318E-6	0.0	4.78785533432657E-5	0.028106792488729285	0.0012594141205511196
18-19	4.163352464631801E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.030823379971901536	0.0013197827312882806
20-21	4.9960229575581604E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.033639887914224946	0.0014259482191363916
22-23	6.036861073716111E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.03628778008173077	0.0015112969446613436
24-25	7.077699189874061E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.03898563247881218	0.0016299524899033497
26-27	7.077699189874061E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.042593177389415635	0.001709056186731354
28-29	7.077699189874061E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.046190313918857515	0.0017402813302160925
30-31	7.077699189874061E-5	4.1633524646318E-6	0.0	7.28586681310565E-5	0.04994565784195539	0.0018443651418318875
32-33	7.077699189874061E-5	4.1633524646318E-6	0.0	8.951207798958371E-5	0.05313478582986335	0.0019692657157708415
34-35	7.077699189874061E-5	4.1633524646318E-6	0.0	9.15937542218996E-5	0.056342648903862154	0.0021087380233360065
36-37	7.077699189874061E-5	4.1633524646318E-6	0.0	9.15937542218996E-5	0.05935275273579095	0.0021711883103054837
38-39	7.077699189874061E-5	4.1633524646318E-6	0.0	9.15937542218996E-5	0.06215260726825583	0.002229475244810329
40-41	7.077699189874061E-5	4.1633524646318E-6	0.0	9.57571066865314E-5	0.0650544639361042	0.002314823970335281
42-43	7.494034436337241E-5	4.1633524646318E-6	0.0	1.0408381161579501E-4	0.06768986604621613	0.0024105810770218127
44-45	7.494034436337241E-5	4.1633524646318E-6	0.0	1.0824716408042681E-4	0.07073535837409428	0.0024980114787790803
46-47	7.702202059568832E-5	4.1633524646318E-6	2.0816762323159E-6	1.1241051654505861E-4	0.07356643805004393	0.002606258642859507
48-49	7.910369682800421E-5	4.1633524646318E-6	4.1633524646318E-6	1.1449219277737452E-4	0.07793587646167499	0.00271658748317225
50-51	8.534872552495191E-5	4.1633524646318E-6	4.1633524646318E-6	1.1657386900969042E-4	0.08101051225680557	0.0027769560939094108
52-53	8.951207798958371E-5	4.1633524646318E-6	4.1633524646318E-6	1.1657386900969042E-4	0.08378330499825035	0.002856059790737415
54-55	9.15937542218996E-5	4.1633524646318E-6	4.1633524646318E-6	1.1865554524200631E-4	0.08650613751011954	0.002918510077706892
56-57	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.2698225017126991E-4	0.08937052400578623	0.0029497352211916303
58-59	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.332272788682176E-4	0.0917103280909093	0.0030205122130903712
60-61	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.332272788682176E-4	0.09421666627461764	0.0031141876435445866
62-63	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.332272788682176E-4	0.09671051440093209	0.0031849646354433275
64-65	9.783878291884731E-5	4.1633524646318E-6	4.1633524646318E-6	1.353089551005335E-4	0.0994999605522354	0.0032453332461804887
66-67	1.020021353834791E-4	4.1633524646318E-6	4.1633524646318E-6	1.373906313328494E-4	0.10241430727747766	0.003336927000402388
68-69	1.0408381161579501E-4	8.3267049292636E-6	4.1633524646318E-6	1.394723075651653E-4	0.10454802541560146	0.0034097856685334446
70-71	1.0616548784811092E-4	8.3267049292636E-6	4.1633524646318E-6	1.4155398379748122E-4	0.10674627551692704	0.003447255840715131
72-73	1.0824716408042681E-4	8.3267049292636E-6	4.1633524646318E-6	1.4155398379748122E-4	0.10894660729448497	0.003513869480149239
74-75	1.1032884031274271E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.11140298524861772	0.0036012998819065075
76-77	1.165738690096904E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.11410500099816376	0.0036720768738052476
78-79	1.228188977066381E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.11696106078890117	0.0037199554271485137
80-81	1.24900573938954E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.11977965040745689	0.0037678339804917793
82-83	1.2906392640358582E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.12253162638657852	0.003799059123976518
84-85	1.394723075651653E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.12515662011552886	0.0038719177921075747
86-87	1.4155398379748122E-4	2.0816762323159003E-5	4.1633524646318E-6	1.4155398379748122E-4	0.1285872225463855	0.0039218780216831554
88-89	1.4363566002979712E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13124552309505289	0.0039843283086526335
90-91	1.45717336262113E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.1334854067210248	0.004032206861995898
92-93	1.477990124944289E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13539014047359382	0.0041279639686824295
94-95	1.4988068872674482E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.1371928720907794	0.004182087550722644
96-97	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13902058382275276	0.0042320477802982245
98-99	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.14051522735755556	0.004282008009873807
100-101	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.1424699213397002	0.0043382132681463355
102-103	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.14408322041974503	0.00438192846902497
104-105	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.477990124944289E-4	0.1455965990406387	0.0044402154035298146
106-107	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.5404404119137664E-4	0.1473493704282487	0.004498502338034661
108-109	1.5820739365600842E-4	2.2898438555474904E-5	4.1633524646318E-6	1.7069745104990382E-4	0.14935194296373655	0.004531809157751715
110-111	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.748608035145356E-4	0.15130039191718425	0.004606749502115088
112-113	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.748608035145356E-4	0.1529428344644815	0.004690016551407723
114-115	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.7902415597916742E-4	0.154395844474638	0.0047503851621448845
116-117	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.7902415597916742E-4	0.15574268899694638	0.004783691981861939
118-119	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	1.8943253714074693E-4	0.15736015142945586	0.004823243830275941
120-121	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	2.0192259453464232E-4	0.1591212495219951	0.004883612441013102
122-123	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	2.0400427076695823E-4	0.16142982846363343	0.0049273276418917355
124-125	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	2.0608594699927414E-4	0.16276834628101255	0.0049647978140734215
126-127	1.6445242235295612E-4	2.4980114787790802E-5	4.1633524646318E-6	2.1857600439316953E-4	0.16414017091810873	0.004993941281325845
128-129	1.686157748175879E-4	2.4980114787790802E-5	4.1633524646318E-6	2.2482103309011723E-4	0.16573681658829503	0.00501683971988132
130-131	1.7277912728221972E-4	2.4980114787790802E-5	4.1633524646318E-6	2.3314773801938083E-4	0.16729807376253195	0.005060554920759954
132-133	1.811058322114833E-4	2.9143467252422604E-5	6.2450286969477005E-6	2.3314773801938083E-4	0.16842217892798256	0.005114678502800167
134-135	1.831875084437992E-4	2.9143467252422604E-5	1.0408381161579502E-5	2.3314773801938083E-4	0.16981273865116955	0.005131331912658694
136-137	1.831875084437992E-4	3.12251434847385E-5	1.2490057393895401E-5	2.3314773801938083E-4	0.17226078990037308	0.005158393703678801
138-139	1.831875084437992E-4	3.33068197170544E-5	1.2490057393895401E-5	2.3731109048401262E-4	0.17394694764854896	0.005181292142234275
>>END_MODULE
