##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24019105
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.025008592118645	37.0	35.0	38.0	33.0	39.0
2	35.4051562287604	35.0	34.0	37.0	33.0	39.0
3	35.51179450691439	36.0	35.0	37.0	33.0	39.0
4	35.61880473897757	36.0	35.0	38.0	33.0	39.0
5	35.53052488841695	36.0	35.0	38.0	33.0	39.0
6	35.497987123167164	36.0	35.0	37.0	33.0	39.0
7	35.40943915270781	35.0	34.0	37.0	33.0	39.0
8	35.57217394236796	36.0	35.0	38.0	33.0	39.0
9	35.55355405623981	36.0	35.0	38.0	33.0	39.0
10-14	35.53061768121668	36.0	35.0	37.8	33.0	39.0
15-19	35.49028718597133	36.0	35.0	38.0	33.0	39.0
20-24	35.465055021825336	36.0	35.0	38.0	33.0	39.0
25-29	35.43162721508566	36.0	35.0	38.0	33.0	39.0
30-34	35.401643433425185	36.0	34.0	38.0	32.8	39.0
35-39	35.37183719376721	36.0	34.0	38.0	32.2	39.0
40-44	35.33475705277111	36.0	34.0	38.0	32.0	39.0
45-49	35.2965462618195	36.0	34.0	38.0	32.0	39.0
50-54	35.25634020085261	36.0	34.0	38.0	32.0	39.0
55-59	35.220355571117246	36.0	34.0	38.0	32.0	39.0
60-64	35.17528191828963	36.0	34.0	38.0	32.0	39.0
65-69	35.12184737940901	36.0	34.0	37.8	32.0	39.0
70-74	35.06329392373279	35.8	34.0	37.6	31.8	39.0
75-79	35.00683081238871	35.2	34.0	37.2	31.0	39.0
80-84	34.945094665267504	35.2	34.0	37.2	31.0	39.0
85-89	34.85914829882296	35.0	34.0	37.0	31.0	39.0
90-94	34.79095278529321	35.0	34.0	37.0	31.0	39.0
95-99	34.725950663024285	35.0	34.0	37.0	30.2	39.0
100-104	34.676621672622694	35.0	34.0	37.0	30.0	39.0
105-109	34.559239946700764	35.0	34.0	37.0	30.0	39.0
110-114	34.486674794918464	35.0	34.0	37.0	30.0	39.0
115-119	34.40284557646923	35.0	34.0	37.0	29.8	39.0
120-124	34.339091918703886	35.0	34.0	37.0	29.0	39.0
125-129	34.19988268505425	35.0	34.0	37.0	29.0	39.0
130-134	34.10944215448494	35.0	34.0	37.0	28.6	39.0
135-139	33.99117681528933	35.0	33.8	37.0	28.0	39.0
140-144	33.84580587827898	35.0	33.0	37.0	28.0	39.0
145-149	33.735941226785926	35.0	33.0	37.0	27.0	39.0
150	33.63286558762285	35.0	33.0	37.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	94.0
21	894.0
22	5911.0
23	24208.0
24	66626.0
25	133493.0
26	224232.0
27	336183.0
28	452026.0
29	556539.0
30	682218.0
31	847416.0
32	1091262.0
33	1480064.0
34	2202208.0
35	3858637.0
36	1.051756E7
37	1539527.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.81994853680018	20.30794236504649	16.742572214909757	28.129536883243567
2	23.583564000407176	25.939817491118006	32.841635856123695	17.634982652351116
3	20.630872798965658	29.119965127759755	31.19346453583512	19.055697537439467
4	23.960663813243666	33.060432518197494	23.837212085962403	19.141691582596437
5	25.015059169522385	35.07968575233977	23.42500341860757	16.480251659530275
6	20.13272767657246	38.87748939854337	23.705483613981453	17.284299310902718
7	20.64979939926987	21.765935908103152	38.558435045768775	19.0258296468582
8	22.400459967180293	23.969069621869757	29.912030444098566	23.71843996685139
9	23.43421205744344	23.291883690087538	29.80884591661513	23.465058335853897
10-14	23.000919476391815	29.595126046536706	26.6018329991896	20.802121477881876
15-19	22.94031105655269	28.421084798954833	28.228854488957854	20.409749655534625
20-24	22.85899412155449	28.61437260047783	27.970281157436965	20.55635212053072
25-29	22.9580455158577	28.54087978276475	27.898325606715318	20.60274909466223
30-34	22.93792379024947	28.359687007488414	28.117360742625507	20.585028459636607
35-39	23.058559425923654	28.42090827281033	27.908307990659935	20.612224310606077
40-44	23.263803543054582	28.122818897706637	27.95814665034355	20.65523090889523
45-49	23.324679250121935	28.05078457336358	27.961984428645447	20.66255174786904
50-54	23.32530208765064	28.040459459251295	27.949755829786334	20.684482623311734
55-59	23.466310672275256	27.842954181681623	27.983020183308245	20.707714962734872
60-64	23.295009535117984	27.764944613881326	28.102231952439528	20.837813898561166
65-69	23.283750997383123	27.838725880918542	28.170331076033015	20.707192045665316
70-74	23.514177568231624	27.749133033891145	28.075764688151374	20.66092470972586
75-79	23.364471740308392	27.749565189876975	28.178456274702988	20.70750679511164
80-84	23.53318743558513	27.820868429527245	27.960949419222743	20.684994715664885
85-89	23.516849607843422	27.773870841565497	28.094688790444106	20.614590760146974
90-94	23.49183452089493	27.794304575461908	28.086436193188714	20.62742471045445
95-99	23.403038803511524	27.93059369168948	28.136968913918675	20.52939859088032
100-104	23.631710673649163	27.85422770748535	27.964986205772448	20.549075413093036
105-109	23.39653871366148	27.934341433621277	28.235796462857383	20.433323389859865
110-114	23.502848253504865	27.885856696159163	28.132474544742614	20.478820505593358
115-119	23.56671907633528	27.89563557842809	28.21138506201626	20.32626028322038
120-124	23.640966638848536	27.899047029437607	28.26094894043721	20.199037391276654
125-129	23.548889935740736	28.158034198193484	28.06657533659143	20.22650052947435
130-134	23.731115709765206	28.111339702291154	28.03343005495001	20.12411453299363
135-139	23.50266922934889	28.15591505178898	28.264192192007155	20.077223526854976
140-144	23.443540464975694	28.25627516096041	28.110120672689508	20.190063701374385
145-149	23.638084130429373	28.466290717046206	27.84508521733083	20.050539935193587
150	23.33591946910595	28.606661239042836	27.880064640210367	20.17735465164085
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	1.5
2	0.5
3	0.5
4	0.5
5	0.5
6	1.0
7	5.5
8	10.0
9	13.0
10	22.0
11	32.0
12	55.0
13	86.0
14	141.0
15	235.0
16	398.0
17	703.0
18	1187.0
19	1885.0
20	2896.0
21	4439.0
22	6634.5
23	10100.0
24	14766.0
25	21752.5
26	41164.0
27	57011.5
28	67077.5
29	92737.5
30	125653.5
31	157159.0
32	201241.0
33	262756.0
34	334453.5
35	423672.0
36	538203.0
37	672352.5
38	810029.0
39	972059.5
40	1157856.5
41	1329086.5
42	1482261.0
43	1582640.0
44	1624846.5
45	1649928.0
46	1618375.0
47	1503405.5
48	1349255.5
49	1163380.0
50	969977.0
51	804594.5
52	655349.5
53	555811.0
54	476226.0
55	376597.0
56	278608.0
57	193906.5
58	131058.0
59	83660.5
60	56733.0
61	44424.5
62	33304.0
63	22284.5
64	15065.0
65	9968.0
66	6472.5
67	4728.0
68	3642.5
69	2803.0
70	2257.0
71	1982.5
72	1739.5
73	1439.5
74	1416.0
75	1375.0
76	948.5
77	421.5
78	186.0
79	94.5
80	26.0
81	8.5
82	4.5
83	4.5
84	6.0
85	4.5
86	3.5
87	2.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.10582409294601111
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0420914934174275
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.007426588126410205
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	8.326704929263601E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4019105E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.2882885908827
#Duplication Level	Percentage of total
1	26.082640823336757
2	9.916965820380259
3	6.397506227921287
4	4.376616289410475
5	3.32861606723063
6	2.6974365309836443
7	2.147627760807566
8	1.7543568762103394
9	1.6254035637707942
>10	19.565053651812295
>50	6.015946973902245
>100	10.578139713988014
>500	2.460321059031462
>1k	2.6075782007474158
>5k	0.34231563232870493
>10k+	0.10347480813827967
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.4980114787790802E-5	4.1633524646318E-6	0.0	0.0	0.001944285600983051	0.0
2	5.828693450484521E-5	4.1633524646318E-6	0.0	0.0	0.012215276131229703	0.0
3	1.9567756583769463E-4	8.3267049292636E-6	0.0	0.0	0.014571733626211301	0.0
4	3.6221166442296666E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.017719228089472943	0.0
5	4.0384518906928465E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.02152869559461104	0.0
6	4.7045882850339343E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.024060013893107173	0.0
7	5.370724679375022E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.026974360618349433	0.0
8	5.953594024423474E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.032715623667076685	0.0
9	8.826307225019417E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.044789345814508906	8.3267049292636E-6
10-11	0.0012635774730157515	8.3267049292636E-6	0.0	1.66534098585272E-5	0.05353446766646801	1.2490057393895401E-5
12-13	0.0012968842927328058	8.3267049292636E-6	0.0	2.0816762323159003E-5	0.05977533301095107	1.66534098585272E-5
14-15	0.0013718246370961781	8.3267049292636E-6	0.0	2.4980114787790802E-5	0.08055878851439302	1.66534098585272E-5
16-17	0.001415539837974812	8.3267049292636E-6	0.0	2.7061791020106703E-5	0.09884839589152053	1.66534098585272E-5
18-19	0.0015924823177216637	8.3267049292636E-6	0.0	2.9143467252422604E-5	0.10544522787172961	1.66534098585272E-5
20-21	0.001896407047639785	8.3267049292636E-6	0.0	2.9143467252422604E-5	0.11355960182529698	1.66534098585272E-5
22-23	0.0022440469784365405	8.3267049292636E-6	0.0	3.33068197170544E-5	0.1220320240908227	1.66534098585272E-5
24-25	0.0022752721219212787	8.3267049292636E-6	0.0	3.9551848414002106E-5	0.12689273809328033	1.66534098585272E-5
26-27	0.0023044155891737018	8.3267049292636E-6	0.0	4.9960229575581604E-5	0.13143079227972898	2.4980114787790802E-5
28-29	0.00254380835589003	8.3267049292636E-6	0.0	4.9960229575581604E-5	0.13613746224099527	2.4980114787790802E-5
30-31	0.0028060995611618334	8.3267049292636E-6	0.0	5.4123582040213406E-5	0.13942651068805434	2.4980114787790802E-5
32-33	0.002849814762040467	8.3267049292636E-6	0.0	5.4123582040213406E-5	0.14307360744707182	2.7061791020106703E-5
34-35	0.0029330818113331033	8.3267049292636E-6	0.0	5.620525827252931E-5	0.14627938884483832	3.33068197170544E-5
36-37	0.0029767970122117375	8.3267049292636E-6	0.0	6.45319632017929E-5	0.14830485981888167	4.163352464631801E-5
38-39	0.0030850441762921644	8.3267049292636E-6	0.0	7.494034436337241E-5	0.1502845339158141	4.78785533432657E-5
40-41	0.0031225143484738503	1.0408381161579502E-5	0.0	8.74304017572678E-5	0.152405761996544	6.036861073716111E-5
42-43	0.0032807217421298588	1.2490057393895401E-5	0.0	9.15937542218996E-5	0.1544124978844965	9.15937542218996E-5
44-45	0.0035367679187047144	1.66534098585272E-5	0.0	9.783878291884731E-5	0.15659409457596357	9.992045915116321E-5
46-47	0.003896897906895365	1.66534098585272E-5	0.0	1.1241051654505861E-4	0.15857376867289602	1.0824716408042681E-4
48-49	0.004073840386642217	1.66534098585272E-5	0.0	1.1865554524200631E-4	0.1603119683268798	1.165738690096904E-4
50-51	0.004471440547014554	1.66534098585272E-5	0.0	1.24900573938954E-4	0.16231245918613538	1.3739063133284943E-4
52-53	0.004792018686791202	1.66534098585272E-5	0.0	1.353089551005335E-4	0.16416931438536114	1.602890698883243E-4
54-55	0.005104270121638587	1.66534098585272E-5	0.0	1.4155398379748122E-4	0.16565771289146702	1.9984091830232642E-4
56-57	0.0053394995358902846	1.66534098585272E-5	0.0	1.4155398379748122E-4	0.16694210712680593	2.393927667163285E-4
58-59	0.0054331749663445	1.66534098585272E-5	0.0	1.4155398379748122E-4	0.16805996726355957	2.664545577364352E-4
60-61	0.005635097560879142	1.66534098585272E-5	0.0	1.4363566002979712E-4	0.1691361938756669	3.080880823827532E-4
62-63	0.006197150143604435	1.66534098585272E-5	0.0	1.477990124944289E-4	0.17026238071734978	3.559666357260189E-4
64-65	0.006992350464349109	1.66534098585272E-5	0.0	1.540440411913766E-4	0.17117207323087186	3.830284267461256E-4
66-67	0.007654323506225564	1.66534098585272E-5	0.0	1.540440411913766E-4	0.17219625793717125	3.9760016037233695E-4
68-69	0.008205967707789278	1.66534098585272E-5	0.0	1.5820739365600842E-4	0.17316007403273354	4.267436276247595E-4
70-71	0.008507810761475085	1.66534098585272E-5	0.0	1.623707461206402E-4	0.17417385035787136	5.037656482204478E-4
72-73	0.009432075008623343	1.66534098585272E-5	0.0	1.7069745104990382E-4	0.17506272610907025	5.495625253313977E-4
74-75	0.010017026029904112	1.66534098585272E-5	0.0	1.7069745104990382E-4	0.1759620102414307	5.662159351899249E-4
76-77	0.010375074341862446	1.66534098585272E-5	0.0	1.7069745104990382E-4	0.17687794778364974	5.974410786746633E-4
78-79	0.010522873354356876	1.66534098585272E-5	0.0	1.7277912728221972E-4	0.1778313554980504	6.245028696947701E-4
80-81	0.010737286006285414	1.66534098585272E-5	0.0	1.811058322114833E-4	0.17870982286808773	6.494829844825608E-4
82-83	0.010893411723709105	1.66534098585272E-5	0.0	1.831875084437992E-4	0.17963408711523599	6.911165091288789E-4
84-85	0.011153621252748594	1.66534098585272E-5	0.0	1.831875084437992E-4	0.1804209607310514	7.265050050782491E-4
86-87	0.011238969978273545	1.66534098585272E-5	0.0	1.8943253714074693E-4	0.18121199769933144	7.618935010276194E-4
88-89	0.011274358474222916	1.66534098585272E-5	0.0	1.915142133730628E-4	0.1822278557007016	8.118537306032011E-4
90-91	0.011278521826687548	1.66534098585272E-5	0.0	1.9567756583769463E-4	0.18319583514872848	8.409971978556236E-4
92-93	0.011288930207849126	1.66534098585272E-5	0.0	2.0400427076695823E-4	0.18409303760485662	8.888757511988895E-4
94-95	0.01129725691277839	1.66534098585272E-5	0.0	2.0400427076695823E-4	0.185044363643025	9.076108372897324E-4
96-97	0.011299338589010706	1.66534098585272E-5	0.0	2.0400427076695823E-4	0.1860893651116476	9.284275996128916E-4
98-99	0.011309746970172285	1.8735086090843102E-5	0.0	2.0608594699927414E-4	0.18726551218290605	9.617344193299459E-4
100-101	0.011313910322636917	2.0816762323159003E-5	0.0	2.123309756962218E-4	0.18855823312317424	0.0010179396776024753
102-103	0.011328482056263129	2.0816762323159003E-5	0.0	2.123309756962218E-4	0.18956784609584743	0.00104083811615795
104-105	0.011336808761192392	2.0816762323159003E-5	0.0	2.2482103309011723E-4	0.19052958051517738	0.0011116151080566906
106-107	0.01135346217105092	2.0816762323159003E-5	0.0	2.3314773801938083E-4	0.19144135470493176	0.001151166956470693
108-109	0.01137427893337408	2.2898438555474904E-5	0.0	2.4355611918096032E-4	0.1923364754848276	0.0012052905385109061
110-111	0.011413830781788082	2.4980114787790802E-5	0.0	2.70617910201067E-4	0.19357299116682325	0.0012469240631572242
112-113	0.011457545982666715	2.4980114787790802E-5	0.0	2.747812626656988E-4	0.19457219575833487	0.0012864759115712263
114-115	0.011476281068757558	2.4980114787790802E-5	0.0	2.789446151303306E-4	0.19544233642344291	0.001317701055055965
116-117	0.011488771126151454	2.4980114787790802E-5	0.0	2.789446151303306E-4	0.1965560332077319	0.0013468445223083873
118-119	0.011513751240939243	2.4980114787790802E-5	0.0	2.8727132005959425E-4	0.19760519802881915	0.0014113764855101804
120-121	0.011522077945868507	2.9143467252422604E-5	0.0	2.8727132005959425E-4	0.19869391469832037	0.0014426016289949188
122-123	0.011524159622100823	2.9143467252422604E-5	0.0	2.893529962919101E-4	0.19988879685566968	0.0015029702397320798
124-125	0.011530404650797771	3.33068197170544E-5	0.0	2.91434672524226E-4	0.20090881820950446	0.0015487671168430298
126-127	0.011532486327030087	3.33068197170544E-5	0.0	2.91434672524226E-4	0.2019454929731978	0.0016153807562771386
128-129	0.01154081303195935	3.33068197170544E-5	0.0	2.976797012211737E-4	0.20310706831083009	0.001673667690781984
130-131	0.011542894708191666	3.5388495949370304E-5	0.0	2.9976137745348964E-4	0.20425823526730075	0.0017132195391959858
132-133	0.011549139736888614	3.7470172181686205E-5	0.0	2.9976137745348964E-4	0.20546560748204395	0.0018110583221148332
134-135	0.011549139736888614	3.7470172181686205E-5	0.0	2.9976137745348964E-4	0.206460648721091	0.001877671961548942
136-137	0.011549139736888614	3.7470172181686205E-5	0.0	3.0392472991812145E-4	0.20759516226770314	0.0019484489534476825
138-139	0.011553303089353246	3.7470172181686205E-5	0.0	3.0600640615043734E-4	0.2086110202690733	0.0020129809166494752
>>END_MODULE
