##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24077269
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.346355103645685	37.0	35.0	38.0	34.0	39.0
2	35.60502052786801	36.0	35.0	38.0	33.0	39.0
3	35.67391580000207	36.0	35.0	37.0	34.0	39.0
4	35.81151728628359	36.0	35.0	38.0	34.0	39.0
5	35.707486509371144	36.0	35.0	38.0	33.0	39.0
6	35.6709582386607	36.0	35.0	37.0	33.0	39.0
7	35.575524657717615	35.0	35.0	37.0	33.0	39.0
8	35.761070867298116	36.0	35.0	38.0	33.0	39.0
9	35.74577714773216	36.0	35.0	38.0	33.0	39.0
10-14	35.71488582862118	36.0	35.0	37.8	33.0	39.0
15-19	35.668886724653035	36.0	35.0	38.0	33.0	39.0
20-24	35.63875922971164	36.0	35.0	38.0	33.0	39.0
25-29	35.596166018662664	36.0	35.0	38.0	33.0	39.0
30-34	35.566267312127465	36.0	35.0	38.0	33.0	39.0
35-39	35.53440598267188	36.0	35.0	38.0	33.0	39.0
40-44	35.496920900788204	36.0	35.0	38.0	33.0	39.0
45-49	35.452309171775255	36.0	35.0	38.0	33.0	39.0
50-54	35.40892015618549	36.0	34.2	38.0	32.6	39.0
55-59	35.37466027397044	36.0	34.0	38.0	32.0	39.0
60-64	35.3195385450127	36.0	34.0	38.0	32.0	39.0
65-69	35.26542548242679	36.0	34.0	38.0	32.0	39.0
70-74	35.20684260711674	36.0	34.0	38.0	32.0	39.0
75-79	35.14691542065759	36.0	34.0	38.0	32.0	39.0
80-84	35.083598171228985	36.0	34.0	38.0	32.0	39.0
85-89	34.99891638239179	36.0	34.0	38.0	31.0	39.0
90-94	34.927499602304565	35.6	34.0	38.0	31.0	39.0
95-99	34.86426931210697	35.2	34.0	37.6	31.0	39.0
100-104	34.8078604138203	35.0	34.0	37.4	31.0	39.0
105-109	34.69188712719591	35.0	34.0	37.0	30.0	39.0
110-114	34.61891792747207	35.0	34.0	37.0	30.0	39.0
115-119	34.5295997579601	35.0	34.0	37.0	30.0	39.0
120-124	34.46362954736888	35.0	34.0	37.0	30.0	39.0
125-129	34.32651750266935	35.0	34.0	37.0	29.0	39.0
130-134	34.233405093131495	35.0	34.0	37.0	29.0	39.0
135-139	34.12106397038237	35.0	34.0	37.0	28.4	39.0
140-144	33.983723620954834	35.0	33.8	37.0	28.0	39.0
145-149	33.953926213532256	35.0	33.0	37.0	28.0	39.0
150	34.878248595389	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	23.0
21	340.0
22	2870.0
23	14220.0
24	45538.0
25	102770.0
26	183455.0
27	288501.0
28	398600.0
29	500503.0
30	618902.0
31	782540.0
32	1024299.0
33	1422242.0
34	2171827.0
35	3918705.0
36	1.1024502E7
37	1577431.0
38	1.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.36141781694593	20.491759260570625	17.046422499163008	28.100400423320437
2	23.627090914135273	25.840394350388923	33.211043711437696	17.32147102403811
3	20.41765066950542	29.17813582622036	31.681149978458922	18.723063525815302
4	23.688039236632065	33.210563800645026	24.13760764796679	18.96378931475612
5	24.903739591682196	35.218479436554304	23.653360721760926	16.224420250002577
6	19.856861673140756	38.94158428017729	24.035554032311556	17.1660000143704
7	20.491755107275665	21.675593689633153	39.0089299579616	18.823721245129587
8	22.16534192478391	23.77691174194216	30.39283234323627	23.664913990037657
9	23.37599417940631	23.19186200062806	30.1500805593857	23.28206326057993
10-14	22.849724360350006	29.60298944203348	26.91371268062005	20.633573516996467
15-19	22.798173663300435	28.41776615113616	28.494714246869112	20.289345938694293
20-24	22.73067333214067	28.644113492713046	28.21007420468321	20.41513897046307
25-29	22.830493555473154	28.595289485272268	28.12866989835468	20.445547060899894
30-34	22.81870173897214	28.410719670906197	28.343496099993732	20.42708249012793
35-39	22.920974135397167	28.459171179256256	28.153829240351136	20.466025444995445
40-44	23.13654094241336	28.176260355773735	28.188496793386324	20.498701908426575
45-49	23.174399887296186	28.113078771516818	28.201758264195163	20.510763076991832
50-54	23.18788895866886	28.115157080315043	28.164170944802752	20.532783016213344
55-59	23.32457057318253	27.91286420399257	28.210860625430566	20.55170459739433
60-64	23.17487183346549	27.851064331717367	28.308269027864792	20.665794806952352
65-69	23.17497333749682	27.905818283502963	28.37035703798047	20.548851341019745
70-74	23.398605451061545	27.82163940664079	28.260179828303528	20.519575313994135
75-79	23.257931060834807	27.83981090800954	28.350113406185194	20.55214462497046
80-84	23.42576086850973	27.907409714970093	28.127324700004337	20.53950471651584
85-89	23.40490863668811	27.863471151694785	28.27146412849854	20.460156083118566
90-94	23.38592388118745	27.858273318389674	28.263519247177584	20.492283553245294
95-99	23.295422360209596	27.98328806676632	28.31231270476608	20.408976868258005
100-104	23.520300891130987	27.916277428929252	28.14269111321263	20.420730566727126
105-109	23.288314497515213	27.98141064021965	28.40415763057848	20.32611723168666
110-114	23.39502524577265	27.951414023642833	28.281258798750486	20.37230193183403
115-119	23.471157418656986	27.945896124476626	28.357627247327528	20.22531920953886
120-124	23.528196924603957	27.94952680391968	28.39590192257579	20.12637434890057
125-129	23.461137504338577	28.167496422937106	28.232042439591797	20.13932363313252
130-134	23.615995362624258	28.135839345528176	28.176272936303636	20.07189235554393
135-139	23.412718864956368	28.16415956555034	28.409883350718278	20.01323821877501
140-144	23.373130036807847	28.227854099991728	28.268932863269324	20.130082999931098
145-149	23.238595784851164	28.080474406721095	28.392735687299343	20.2881941211284
150	23.833369987759333	26.970165614945756	28.48969963974215	20.706764757552758
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.5
3	1.0
4	1.0
5	1.5
6	2.0
7	4.0
8	9.5
9	18.0
10	32.5
11	56.0
12	82.5
13	112.5
14	201.5
15	348.0
16	543.0
17	864.5
18	1433.5
19	2371.5
20	3651.5
21	5584.0
22	8297.0
23	12233.5
24	17538.5
25	25820.5
26	45542.5
27	62886.5
28	76537.0
29	103928.5
30	137619.0
31	172700.0
32	220128.0
33	283310.5
34	359013.5
35	452757.0
36	570324.0
37	706613.5
38	848589.5
39	1012980.5
40	1197677.5
41	1365385.0
42	1514047.5
43	1610700.5
44	1642809.0
45	1652110.5
46	1606144.0
47	1479529.5
48	1313414.0
49	1123761.5
50	931806.0
51	764966.5
52	618010.0
53	516788.0
54	435567.5
55	343535.5
56	255316.0
57	178509.5
58	121259.0
59	78041.5
60	53560.0
61	41825.5
62	31197.0
63	20787.5
64	13824.5
65	9431.0
66	6237.5
67	4398.0
68	3363.5
69	2525.0
70	1930.5
71	1522.5
72	1178.5
73	946.0
74	1019.5
75	987.5
76	647.0
77	306.5
78	144.5
79	73.5
80	25.5
81	5.0
82	3.5
83	3.5
84	4.5
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.153294960487421E-6
3	7.475930928877357E-5
4	7.351332080062735E-4
5	0.133225242447555
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	6.645271936779873E-6
25-29	0.05220608699433478
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.307677839551824E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	2.0
64-65	4.0
66-67	6.0
68-69	5.0
70-71	15.0
72-73	23.0
74-75	25.0
76-77	32.0
78-79	45.0
80-81	73.0
82-83	94.0
84-85	89.0
86-87	129.0
88-89	140.0
90-91	197.0
92-93	190.0
94-95	254.0
96-97	235.0
98-99	298.0
100-101	389.0
102-103	318.0
104-105	402.0
106-107	396.0
108-109	404.0
110-111	482.0
112-113	471.0
114-115	558.0
116-117	599.0
118-119	660.0
120-121	735.0
122-123	809.0
124-125	846.0
126-127	1058.0
128-129	1159.0
130-131	1407.0
132-133	1758.0
134-135	2181.0
136-137	2982.0
138-139	5223.0
140-141	8620.0
142-143	12387.0
144-145	61906.0
146-147	727816.0
148-149	1093543.0
150-151	2.2148303E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.61858478258496
#Duplication Level	Percentage of total
1	27.621526826127035
2	10.968466912103757
3	6.79620973245539
4	4.7404169046926565
5	3.45990237280285
6	2.812356960680473
7	2.3181940119574658
8	1.8875879270101876
9	1.5888569223051787
>10	18.7914453745733
>50	5.587003090903223
>100	9.143597490954756
>500	2.0012748838577723
>1k	1.9939070088011213
>5k	0.24577838221682427
>10k+	0.04347519855799155
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0766474802437104E-5	0.0	0.0	4.153294960487421E-6	0.0014744197109730344	0.0
2	6.22994244073113E-5	0.0	0.0	1.2459884881462262E-5	0.010703041113176083	4.153294960487421E-6
3	1.57825208498522E-4	0.0	0.0	1.2459884881462262E-5	0.013045499470890988	8.306589920974842E-6
4	2.9488394219460686E-4	0.0	0.0	2.0766474802437104E-5	0.01602341195756047	1.2459884881462262E-5
5	3.5303007164143074E-4	0.0	0.0	2.0766474802437104E-5	0.019545406084053803	1.2459884881462262E-5
6	4.028696111672798E-4	0.0	0.0	2.0766474802437104E-5	0.02198339022585992	1.6613179841949684E-5
7	4.4855585573264144E-4	0.0	0.0	2.4919769762924524E-5	0.02463734570561138	2.0766474802437104E-5
8	5.025486902189779E-4	0.0	0.0	2.4919769762924524E-5	0.030123848348415264	2.0766474802437104E-5
9	8.098925172950471E-4	0.0	0.0	2.4919769762924524E-5	0.041063627274339126	2.0766474802437104E-5
10-11	0.0012376818982252514	0.0	0.0	2.6996417243168236E-5	0.05040023434551485	2.6996417243168236E-5
12-13	0.0012937513801918317	0.0	0.0	4.153294960487421E-5	0.05707457934701814	2.9073064723411944E-5
14-15	0.001370587336960849	0.0	0.0	5.399283448633647E-5	0.07734265875419674	2.9073064723411944E-5
16-17	0.0013996604016842608	0.0	0.0	5.606948196658018E-5	0.09516860072460875	3.322635968389937E-5
18-19	0.0016530113942739935	0.0	0.0	6.437607188755501E-5	0.10147953241706939	3.7379654644386784E-5
20-21	0.0020018881709549367	0.0	0.0	7.060601432828615E-5	0.10880594472736921	3.94563021246305E-5
22-23	0.002365301479997586	0.0	0.0	7.475930928877358E-5	0.11611159056286657	4.153294960487421E-5
24-25	0.002396451192201242	0.0	0.0	8.306589920974841E-5	0.12101040196876149	4.153294960487421E-5
26-27	0.0024462907317270906	0.0	0.0	8.721919417023583E-5	0.12552711023829155	4.153294960487421E-5
28-29	0.0026934117818760922	0.0	0.0	8.721919417023583E-5	0.13071872893890085	4.153294960487421E-5
30-31	0.0029654526017880182	4.153294960487421E-6	0.0	8.721919417023583E-5	0.13413896733886221	4.5686244565361624E-5
32-33	0.002998678961471918	4.153294960487421E-6	0.0	9.96790790516981E-5	0.13778971360913067	4.983953952584905E-5
34-35	0.0031149712203655654	4.153294960487421E-6	0.0	1.0798566897267294E-4	0.14113311605232304	5.399283448633647E-5
36-37	0.003148197580049465	4.153294960487421E-6	0.0	1.1629225889364778E-4	0.14346311452515648	6.22994244073113E-5
38-39	0.0032250335368184824	4.153294960487421E-6	0.0	1.1629225889364778E-4	0.14582218606271335	7.8912604249261E-5
40-41	0.0032769497238245753	4.153294960487421E-6	0.0	1.1836890637389148E-4	0.14818125760027018	8.929584165047954E-5
42-43	0.003490844414289677	4.153294960487421E-6	0.0	1.245988488146226E-4	0.1503139745624805	9.760243157145439E-5
44-45	0.0038480277808915953	4.153294960487421E-6	0.0	1.245988488146226E-4	0.15269173592735952	1.1006231645291665E-4
46-47	0.00411383865836279	4.153294960487421E-6	0.0	1.245988488146226E-4	0.1550466541699559	1.2459884881462264E-4
48-49	0.004255050687019363	4.153294960487421E-6	0.0	1.2667549629486634E-4	0.15710461182287744	1.4536532361705972E-4
50-51	0.004610157406141038	4.153294960487421E-6	0.0	1.2875214377511005E-4	0.1591604928283187	1.7443838834047166E-4
52-53	0.00488635152101345	4.153294960487421E-6	0.0	1.3290543873559747E-4	0.16128697984808826	1.993581581033962E-4
54-55	0.005091939621557578	4.153294960487421E-6	0.0	1.391353811763286E-4	0.16281539239354764	2.3050787030705187E-4
56-57	0.005258071419977075	4.153294960487421E-6	0.0	1.412120286565723E-4	0.1642918887520009	2.6996417243168236E-4
58-59	0.005334907376746092	4.153294960487421E-6	0.0	1.4536532361705972E-4	0.16570193239108638	3.1772706447728773E-4
60-61	0.0054968858802051015	4.153294960487421E-6	0.0	1.4536532361705972E-4	0.16704759995828433	3.966396687265487E-4
62-63	0.006124033419238702	6.229942440731132E-6	0.0	1.4536532361705972E-4	0.16831227827375272	4.5686244565361627E-4
64-65	0.006902776224330093	8.306589920974842E-6	0.0	1.57825208498522E-4	0.16932152894915117	5.108552801399527E-4
66-67	0.007451011159114433	8.306589920974842E-6	0.0	1.6405515093925312E-4	0.1704823748906074	5.586181721855581E-4
68-69	0.00788087718752488	8.306589920974842E-6	0.0	1.7859168330095908E-4	0.1714937022134861	6.084577117114072E-4
70-71	0.00814876471247632	8.306589920974842E-6	0.0	1.9105156818242136E-4	0.17245726664431915	6.936002584013993E-4
72-73	0.009018880006698433	1.2459884881462262E-5	0.0	2.035114530638836E-4	0.17334607176586347	7.621296252494417E-4
74-75	0.009415519675424982	1.2459884881462262E-5	0.0	2.035114530638836E-4	0.1742681032470917	7.891260424926099E-4
76-77	0.009681330552896179	1.2459884881462262E-5	0.0	2.035114530638836E-4	0.17532719346201597	8.098925172950471E-4
78-79	0.00982254258155275	1.2459884881462262E-5	0.0	2.035114530638836E-4	0.1765731819501622	8.576554093406524E-4
80-81	0.010046820509419071	1.2459884881462262E-5	0.0	2.0558810054412732E-4	0.1775180565536731	8.88805121544308E-4
82-83	0.010200492422957105	1.2459884881462262E-5	0.0	2.0766474802437102E-4	0.17850446410678886	9.137248913072325E-4
84-85	0.010426846998303669	1.2459884881462262E-5	0.0	2.0766474802437102E-4	0.17940780576069487	9.386446610701571E-4
86-87	0.010524449429875123	1.2459884881462262E-5	0.0	2.0974139550461473E-4	0.1803443737742848	9.718710207540565E-4
88-89	0.0105327560197961	1.4536532361705972E-5	0.0	2.1181804298485847E-4	0.18127886514039443	0.0010300171502008802
90-91	0.0105327560197961	1.6613179841949684E-5	0.0	2.201246329058333E-4	0.1823275721179175	0.0010840099846872167
92-93	0.010549369199638049	2.0766474802437104E-5	0.0	2.2635457534656443E-4	0.18329944313867158	0.0011525393515352594
94-95	0.010549369199638049	2.0766474802437104E-5	0.0	2.2843122282680813E-4	0.18420901473501833	0.0011940723011401335
96-97	0.010551445847118292	2.0766474802437104E-5	0.0	2.3050787030705184E-4	0.185305484604587	0.0012210687183833017
98-99	0.010559752437039268	2.0766474802437104E-5	0.0	2.4296775518851412E-4	0.18655770303517397	0.0012646783154684196
100-101	0.010570135674440486	2.0766474802437104E-5	0.0	2.450444026687578E-4	0.18768739926442654	0.0012937513801918317
102-103	0.010574288969400974	2.4919769762924524E-5	0.0	2.450444026687578E-4	0.18852221155148452	0.0013477442146781681
104-105	0.010590902149242923	2.4919769762924524E-5	0.0	2.533509925897327E-4	0.18959583829877052	0.0014162735815262104
106-107	0.01061166862404536	2.9073064723411944E-5	0.0	2.616575825107075E-4	0.1904410338232297	0.0014764963584532782
108-109	0.010617898566486091	3.114971220365565E-5	0.0	2.6996417243168236E-4	0.19142328808138498	0.001563715552623514
110-111	0.010636588393808283	3.5303007164143076E-5	0.0	3.1357376951680026E-4	0.1925986705552029	0.0016114784446691192
112-113	0.010653201573650234	4.3609597085117916E-5	0.0	3.2603365439826256E-4	0.193562234986036	0.0016966209913591114
114-115	0.010665661458531695	4.568624456536163E-5	0.0	3.2811030187850624E-4	0.19456317907151346	0.0017693036531676413
116-117	0.01067812134341316	4.983953952584905E-5	0.0	3.2811030187850624E-4	0.1957136417755685	0.0018232964876539778
118-119	0.01068435128585389	4.983953952584905E-5	0.0	3.3226359683899366E-4	0.19668343614884232	0.001900132444422995
120-121	0.010698887818215597	4.983953952584905E-5	0.0	3.3849353927972476E-4	0.19773006647888514	0.0019665851637907937
122-123	0.01070096446569584	5.191618700609276E-5	0.0	3.405701867599685E-4	0.19903212444899793	0.00203719117811908
124-125	0.010703041113176083	5.606948196658018E-5	0.0	3.447234817204559E-4	0.20007667813156052	0.002091184012605416
126-127	0.010711347703097058	5.814612944682389E-5	0.0	3.5303007164143074E-4	0.2011066952817614	0.0021700966168546774
128-129	0.010711347703097058	5.814612944682389E-5	0.0	3.5718336660191816E-4	0.20231530411526324	0.0022448559261434506
130-131	0.010715500998057546	5.814612944682389E-5	0.0	3.5718336660191816E-4	0.20352183630128484	0.0023030020555902747
132-133	0.010717577645537789	5.814612944682389E-5	0.0	3.5718336660191816E-4	0.204622459465814	0.002381914659839536
134-135	0.010719654293018032	5.814612944682389E-5	0.0	3.6133666156240563E-4	0.20550711129239782	0.0024795170914109904
136-137	0.010727960882939008	6.22994244073113E-5	0.0	3.8002648888459903E-4	0.206684570413696	0.0025584296956602513
138-139	0.010727960882939008	6.437607188755501E-5	0.0	3.821031363648427E-4	0.2076398282546081	0.002631112357468781
>>END_MODULE
