##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.2.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24177239
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00839181843717	37.0	35.0	38.0	33.0	39.0
2	35.20443471647031	35.0	34.0	38.0	32.0	39.0
3	35.40155755584829	36.0	34.0	38.0	32.0	39.0
4	35.40374825264374	36.0	35.0	38.0	32.0	39.0
5	35.32518998550662	36.0	35.0	38.0	32.0	39.0
6	35.24017626661175	36.0	34.0	37.0	33.0	39.0
7	35.08447536130986	35.0	34.0	37.0	33.0	39.0
8	35.26228908933729	35.0	34.0	37.0	33.0	39.0
9	35.22442322715178	35.0	34.0	37.0	32.0	39.0
10-14	35.2623614218315	35.4	34.2	37.4	32.4	39.0
15-19	35.24040866701115	36.0	34.0	37.0	32.0	39.0
20-24	35.24058155689324	36.0	34.0	37.0	32.0	39.0
25-29	35.2310502617772	36.0	34.0	37.0	32.0	39.0
30-34	35.20412687321327	36.0	34.0	37.0	32.0	39.0
35-39	35.17266686241551	35.8	34.0	37.0	32.0	39.0
40-44	35.15122740855562	35.8	34.0	37.0	32.0	39.0
45-49	35.13755656714979	35.8	34.0	37.0	32.0	39.0
50-54	35.109765279650006	35.2	34.0	37.0	32.0	39.0
55-59	35.08662962714642	35.0	34.0	37.0	32.0	39.0
60-64	35.06261874649955	35.0	34.0	37.0	32.0	39.0
65-69	35.02964930002686	35.0	34.0	37.0	32.0	39.0
70-74	35.015506379447814	35.0	34.0	37.0	32.0	39.0
75-79	34.983686804860326	35.0	34.0	37.0	32.0	39.0
80-84	34.9612608338007	35.0	34.0	37.0	32.0	39.0
85-89	34.92929991070101	35.0	34.0	37.0	32.0	39.0
90-94	34.900231178750175	35.0	34.0	37.0	31.0	39.0
95-99	34.8582122465981	35.0	34.0	37.0	31.0	39.0
100-104	34.829283314654205	35.0	34.0	37.0	31.0	39.0
105-109	34.80457061661979	35.0	34.0	37.0	31.0	39.0
110-114	34.75601437055921	35.0	34.0	37.0	31.0	39.0
115-119	34.730063458680974	35.0	34.0	37.0	31.0	39.0
120-124	34.69082602496103	35.0	34.0	37.0	31.0	39.0
125-129	34.64574671417824	35.0	34.0	37.0	30.8	39.0
130-134	34.60457858147278	35.0	34.0	37.0	30.2	39.0
135-139	34.55701022155391	35.0	34.0	37.0	30.0	39.0
140-144	34.516694213384696	35.0	34.0	37.0	30.0	39.0
145-149	34.51195029807842	35.0	34.0	37.0	30.0	39.0
150	35.195099808344075	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	59.0
23	793.0
24	6954.0
25	34490.0
26	106687.0
27	233458.0
28	385793.0
29	531357.0
30	686967.0
31	897763.0
32	1209310.0
33	1702459.0
34	2585350.0
35	4552970.0
36	1.0174985E7
37	1067821.0
38	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.58607333120213	11.825163328203026	15.717419180908127	36.87134415968672
2	22.30207828809978	12.750800240392667	34.217964843606204	30.72915662790135
3	21.238496070552813	17.4914738410142	27.54146129813524	33.72856879029775
4	24.476753174977464	24.91504123212528	22.151632239901176	28.456573352996077
5	24.297031440153003	31.63995796514194	24.18536781762328	19.87764277708178
6	18.788339228650983	37.90148999278119	25.246360222652044	18.06381055591579
7	14.844359339008381	26.68989777048657	40.184221668887986	18.281521221617062
8	17.463135357530117	26.304784495209848	31.77469701075762	24.457383136502415
9	18.111680291847023	24.71281649332735	32.95498003271698	24.220523182108654
10-14	19.084665356916297	30.88822295269719	27.467194846359998	22.55991684402651
15-19	19.24182553625035	29.198537272757342	28.455307042730098	23.10433014826221
20-24	19.309198395700616	29.362684003480748	28.115030616141233	23.2130869846774
25-29	19.460710298906886	29.42426211357948	27.929426988665	23.185600598848634
30-34	19.34838855119998	29.370291711466546	27.894355986634388	23.38696375069909
35-39	19.437441115377666	29.168633365039025	28.079089842862636	23.314835676720673
40-44	19.406422394865324	29.275915466668284	28.01012865046833	23.307533487998064
45-49	19.539193085981264	29.09629478155409	28.005577817578363	23.35893431488628
50-54	19.523780488163005	29.164928767410913	27.999453633509635	23.311837110916443
55-59	19.616150760612292	29.081248997298264	27.937035302496575	23.36556493959287
60-64	19.626823247925337	29.01216333701275	27.885654769894874	23.475358645167034
65-69	19.599696308843782	28.87760175974102	28.06223997706268	23.460461954352517
70-74	19.689304400893676	28.887774450842702	28.005313566484823	23.4176075817788
75-79	19.548696572178756	28.91654763297456	28.017060081312017	23.517695713534664
80-84	19.770482474337875	28.748532955844684	27.913652516655123	23.567332053162314
85-89	19.722396916852215	28.81041401600167	28.03316062313323	23.43402844401289
90-94	19.890524223852402	28.664414557316743	27.951307352042047	23.49375386678881
95-99	19.92906852385676	28.692114317729718	27.97044975703927	23.40836740137425
100-104	19.9451671543942	28.586917596451134	27.95300112711593	23.514914122038736
105-109	19.87507339555687	28.586722505721205	28.028959290421955	23.509244808299965
110-114	19.9684339102741	28.60434660958956	27.930801687316926	23.49641779281942
115-119	20.03325808712905	28.558449828202864	27.997899207092637	23.410392877575443
120-124	20.0442520770089	28.403080868198828	28.09373526256479	23.45893179222748
125-129	20.133424411393804	28.41849920395217	28.002057736087743	23.44601864856628
130-134	20.04153426777714	28.54000190364015	27.970027553970905	23.448436274611797
135-139	20.123408467861104	28.390935393898946	27.977767456696554	23.507888681543392
140-144	20.20530976995885	28.31889090204619	28.06345219126572	23.41234713672924
145-149	19.89482139563389	28.38590914543933	28.245209600868808	23.47405985805797
150	20.25281650261375	27.293788469911302	28.321342388257627	24.132052639217328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	3.0
7	4.5
8	7.5
9	10.5
10	16.5
11	40.5
12	76.5
13	145.0
14	244.5
15	413.5
16	760.0
17	1463.0
18	3390.5
19	6854.0
20	8262.0
21	9713.0
22	14203.5
23	20499.5
24	30276.0
25	42615.0
26	56833.5
27	77148.5
28	106102.0
29	144570.5
30	190989.5
31	244038.0
32	299494.0
33	358961.5
34	427602.0
35	511817.5
36	611272.5
37	724155.5
38	849784.5
39	988955.5
40	1132067.0
41	1272087.5
42	1404845.0
43	1510746.0
44	1586134.0
45	1612714.0
46	1563042.0
47	1464741.5
48	1323775.0
49	1148216.5
50	966437.5
51	792537.5
52	638464.0
53	512582.5
54	416747.5
55	325427.0
56	242762.0
57	171160.5
58	111470.5
59	72139.5
60	50399.0
61	38287.0
62	28576.0
63	19271.0
64	12116.0
65	8171.5
66	5515.5
67	3693.0
68	2363.0
69	1596.5
70	1549.5
71	1856.0
72	1939.5
73	1413.5
74	859.5
75	484.0
76	216.5
77	82.0
78	19.0
79	5.5
80	5.5
81	4.5
82	3.0
83	3.5
84	4.5
85	4.0
86	2.0
87	1.5
88	2.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.335596839655677E-4
3	7.196851551163472E-4
4	8.975383831048698E-4
5	6.70051696142806E-4
6	5.873292645202375E-4
7	7.031406687918334E-4
8	5.914653861013658E-4
9	7.817269788332738E-4
10-14	8.876116913101615E-4
15-19	6.394443964424557E-4
20-24	6.435805180235841E-4
25-29	7.36229641440861E-4
30-34	3.664603720879791E-4
35-39	3.5736090460949656E-4
40-44	1.646176389289116E-4
45-49	1.6958098482626574E-4
50-54	2.514761921326087E-4
55-59	2.4568562191902885E-4
60-64	1.7206265777494278E-4
65-69	1.83643802759541E-4
70-74	1.149841970765607E-4
75-79	1.5965437227354485E-4
80-84	6.452359541025466E-5
85-89	1.6709993114407442E-4
90-94	7.858690162330344E-5
95-99	8.02417822219335E-5
100-104	6.204304008414691E-5
105-109	1.2656886345900742E-4
110-114	6.287138894661751E-5
115-119	1.3318968260766272E-4
120-124	1.6131889163597036E-4
125-129	1.0589306575721952E-4
130-134	1.0175901796690314E-4
135-139	1.4147640172791832E-4
140-144	1.6219985909135507E-4
145-149	8.396306471500233E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	0.0
70-71	2.0
72-73	3.0
74-75	3.0
76-77	5.0
78-79	8.0
80-81	16.0
82-83	14.0
84-85	21.0
86-87	32.0
88-89	38.0
90-91	35.0
92-93	49.0
94-95	48.0
96-97	68.0
98-99	59.0
100-101	70.0
102-103	81.0
104-105	81.0
106-107	86.0
108-109	83.0
110-111	96.0
112-113	105.0
114-115	138.0
116-117	109.0
118-119	141.0
120-121	134.0
122-123	124.0
124-125	170.0
126-127	173.0
128-129	196.0
130-131	278.0
132-133	318.0
134-135	439.0
136-137	623.0
138-139	1579.0
140-141	3602.0
142-143	5890.0
144-145	48728.0
146-147	617043.0
148-149	1006246.0
150-151	2.2490304E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.571331314391216
#Duplication Level	Percentage of total
1	37.08735695409674
2	11.096974035519422
3	6.228336610580836
4	4.398231909477922
5	3.1508766826767136
6	2.506419966965443
7	2.073524645990131
8	1.7269639740024965
9	1.545204476436723
>10	16.859037835585386
>50	4.620103469034708
>100	6.673315735195262
>500	1.189731492778706
>1k	0.8439222116593835
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	1.654448632451373E-4	1.6958098482626572E-4
2	0.0	0.0	0.0	4.1361215811284326E-6	0.0016337680245457308	2.8952851067899025E-4
3	4.1361215811284326E-6	0.0	0.0	4.1361215811284326E-6	0.003089682821102939	3.7638706388268733E-4
4	1.2408364743385298E-5	0.0	0.0	4.1361215811284326E-6	0.004835126128339137	4.425650091807423E-4
5	2.4816729486770595E-5	0.0	0.0	4.1361215811284326E-6	0.00659711392189985	4.6738173866751285E-4
6	2.4816729486770595E-5	0.0	0.0	4.1361215811284326E-6	0.007689050019317756	4.963345897354118E-4
7	3.308897264902746E-5	0.0	0.0	4.1361215811284326E-6	0.008888525277845002	5.17015197641054E-4
8	3.308897264902746E-5	0.0	0.0	4.1361215811284326E-6	0.010704282651960382	5.749208997768521E-4
9	3.7225094230155895E-5	0.0	0.0	4.1361215811284326E-6	0.014083493983742312	6.493710882371639E-4
10-11	4.136121581128432E-5	0.0	0.0	4.1361215811284326E-6	0.018773855856741954	7.755227964615811E-4
12-13	4.136121581128432E-5	0.0	0.0	4.1361215811284326E-6	0.02221304095145025	8.99606443895434E-4
14-15	4.342927660184854E-5	0.0	0.0	6.204182371692649E-6	0.026140288392731692	0.0010050775442142091
16-17	4.5497337392412756E-5	0.0	0.0	2.2748668696206378E-5	0.029498819116607983	0.0011229570092763696
18-19	5.790570213579805E-5	0.0	2.0680607905642163E-6	2.4816729486770595E-5	0.03222245517778105	0.0012118836232706308
20-21	6.410988450749071E-5	0.0	4.1361215811284326E-6	3.7225094230155895E-5	0.03533075054599907	0.001300810237264892
22-23	7.031406687918335E-5	0.0	4.1361215811284326E-6	3.7225094230155895E-5	0.0380564546679627	0.0013814646080968964
24-25	7.031406687918335E-5	0.0	4.1361215811284326E-6	3.7225094230155895E-5	0.04067461962881701	0.0014931398907873641
26-27	7.031406687918335E-5	0.0	4.1361215811284326E-6	3.929315502072011E-5	0.04422754806700632	0.001577930383200497
28-29	7.031406687918335E-5	0.0	4.1361215811284326E-6	4.136121581128432E-5	0.04795212555081248	0.0016503125108702445
30-31	7.6518249250876E-5	0.0	4.1361215811284326E-6	4.342927660184854E-5	0.05175322128386951	0.0017433752464456342
32-33	7.858631004144022E-5	0.0	4.1361215811284326E-6	4.756539818297697E-5	0.055219291168855135	0.0018757311370417441
34-35	7.858631004144022E-5	0.0	4.1361215811284326E-6	4.963345897354119E-5	0.058482691096365474	0.002037039878705753
36-37	7.858631004144022E-5	0.0	4.1361215811284326E-6	5.790570213579805E-5	0.06159925870774574	0.0021094220063755005
38-39	7.858631004144022E-5	0.0	4.1361215811284326E-6	6.204182371692648E-5	0.0646186274619695	0.0021549193437679134
40-41	7.858631004144022E-5	0.0	4.1361215811284326E-6	6.824600608861913E-5	0.0676690171280517	0.00224177789697161
42-43	7.858631004144022E-5	0.0	4.1361215811284326E-6	7.445018846031179E-5	0.07058084672116613	0.002343112875709257
44-45	8.272243162256864E-5	0.0	4.1361215811284326E-6	7.445018846031179E-5	0.07360435159697101	0.0024485839760280317
46-47	8.272243162256864E-5	8.272243162256865E-6	4.1361215811284326E-6	9.513079636595394E-5	0.07663612871593817	0.0025354425292317294
48-49	8.685855320369709E-5	1.4476425533949513E-5	4.1361215811284326E-6	9.513079636595394E-5	0.08055717197484791	0.0026367775079693756
50-51	9.306273557538973E-5	1.654448632451373E-5	4.1361215811284326E-6	9.926691794708238E-5	0.08358688103302449	0.0027112276964296874
52-53	9.513079636595394E-5	1.654448632451373E-5	4.1361215811284326E-6	9.926691794708238E-5	0.08624433914889951	0.0027794737025183067
54-55	9.513079636595394E-5	1.654448632451373E-5	4.1361215811284326E-6	1.013349787376466E-4	0.08904035733774232	0.002843583587025797
56-57	9.513079636595394E-5	2.068060790564216E-5	4.1361215811284326E-6	1.1167528269046768E-4	0.091960459174019	0.0029118295931144164
58-59	9.513079636595394E-5	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.0943304568400056	0.002994552024736985
60-61	9.513079636595394E-5	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.09701066362457682	0.0030586619092444756
62-63	9.926691794708238E-5	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.09945304341823316	0.0031641330095632508
64-65	1.0547110031877502E-4	2.068060790564216E-5	4.1361215811284326E-6	1.158114042715961E-4	0.10203605134564786	0.003236515137232998
66-67	1.0753916110933924E-4	2.4816729486770595E-5	4.1361215811284326E-6	1.158114042715961E-4	0.10472039425180021	0.0032882166569971035
68-69	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.1787946506216032E-4	0.10708211967462455	0.0033730071494102367
70-71	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2408364743385296E-4	0.10949761467800356	0.0034267767299649065
72-73	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2615170822441717E-4	0.11175180093971855	0.0035363839518648098
74-75	1.0753916110933924E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.11446096057535768	0.0036232425050685066
76-77	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1170605129890969	0.003703896875900511
78-79	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1198627353603114	0.0037452580917117957
80-81	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.12264634518441084	0.003801095733057029
82-83	1.158114042715961E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.12527691851000852	0.0038569333744022634
84-85	1.2201558664328876E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.12804811996936455	0.0038941584686324193
86-87	1.3028782980554563E-4	2.8952851067899026E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.13118743624944107	0.003970676717883296
88-89	1.3442395138667406E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1337745802984369	0.004059603331877557
90-91	1.3649201217723827E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1364258342319402	0.004109236790851097
92-93	1.3649201217723827E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.13859729806203264	0.004185755040101973
94-95	1.3649201217723827E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.14058884060334598	0.004262273289352849
96-97	1.406281337583667E-4	3.308897264902746E-5	4.1361215811284326E-6	1.2821976901498141E-4	0.1425224774425235	0.004328451234650904
98-99	1.406281337583667E-4	3.308897264902746E-5	4.1361215811284326E-6	1.3235589059610984E-4	0.14419553862208997	0.00435533602492824
100-101	1.406281337583667E-4	3.5157033439591674E-5	4.1361215811284326E-6	1.3649201217723827E-4	0.14612917546126752	0.00439049305836783
102-103	1.4476425533949512E-4	4.136121581128432E-5	4.1361215811284326E-6	1.3856007296780248E-4	0.14789116325482823	0.00445873906445645
104-105	1.4476425533949512E-4	4.136121581128432E-5	4.1361215811284326E-6	1.4476425533949512E-4	0.14959317728546256	0.004489759976314914
106-107	1.4683231613005937E-4	4.342927660184854E-5	4.1361215811284326E-6	1.4476425533949512E-4	0.15132207610637427	0.004541461496079018
108-109	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.5510455929231622E-4	0.15330121028294422	0.004584890772680867
110-111	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.5924068087344465E-4	0.15562984673311953	0.004686225751418514
112-113	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.654448632451373E-4	0.15753866684281032	0.004764812061459954
114-115	1.4890037692062358E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.6958098482626572E-4	0.15913520977312587	0.004808241338061803
116-117	1.53036498501752E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.6958098482626572E-4	0.1607110720955358	0.004824785824386316
118-119	1.53036498501752E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.7164904561682993E-4	0.16244824315960973	0.004859942857825908
120-121	1.5924068087344465E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.778532279885226E-4	0.16427847695925907	0.004909576316799449
122-123	1.6130874166400886E-4	4.5497337392412756E-5	4.1361215811284326E-6	1.8198934956965103E-4	0.16643753242460813	0.004938529167867349
124-125	1.6130874166400886E-4	4.963345897354119E-5	4.1361215811284326E-6	1.8819353194134367E-4	0.16794308068013886	0.004955073654191861
126-127	1.675129240357015E-4	4.963345897354119E-5	4.1361215811284326E-6	1.943977143130363E-4	0.16938245099037155	0.0049695500797258116
128-129	1.7164904561682993E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.17114443878393226	0.004996434870003147
130-131	1.7578516719795838E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.17294778779330428	0.005025387721071046
132-133	1.881935319413437E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.1742403257874069	0.005062612815301201
134-135	2.0060189668472898E-4	4.963345897354119E-5	4.1361215811284326E-6	1.9853383589416476E-4	0.17581618810981683	0.005114314335065307
136-137	2.026699574752932E-4	4.963345897354119E-5	4.1361215811284326E-6	2.047380182658574E-4	0.17812828007366763	0.005149471368504898
138-139	2.026699574752932E-4	4.963345897354119E-5	4.1361215811284326E-6	2.1507832221867847E-4	0.1797723884021662	0.005166015854829412
>>END_MODULE
