##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24177239
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	58-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.24378375049359	37.0	35.0	38.0	33.0	39.0
2	35.500551158881294	36.0	35.0	38.0	33.0	39.0
3	35.57233669237418	36.0	35.0	37.0	33.0	39.0
4	35.72229256616109	36.0	35.0	38.0	33.0	39.0
5	35.59864941567563	36.0	35.0	38.0	33.0	39.0
6	35.578448018816374	36.0	35.0	37.0	33.0	39.0
7	35.47453015623496	35.0	34.0	37.0	33.0	39.0
8	35.66625634961875	36.0	35.0	38.0	33.0	39.0
9	35.64990150446873	36.0	35.0	38.0	33.0	39.0
10-14	35.610146336395154	36.0	35.0	37.8	33.0	39.0
15-19	35.56024791747312	36.0	35.0	38.0	33.0	39.0
20-24	35.53089239015257	36.0	35.0	38.0	33.0	39.0
25-29	35.48006025005585	36.0	35.0	38.0	33.0	39.0
30-34	35.45426695744705	36.0	35.0	38.0	33.0	39.0
35-39	35.41859295844326	36.0	34.8	38.0	32.8	39.0
40-44	35.377027062519424	36.0	34.0	38.0	32.0	39.0
45-49	35.33413285114979	36.0	34.0	38.0	32.0	39.0
50-54	35.288150809941534	36.0	34.0	38.0	32.0	39.0
55-59	35.24957222976186	36.0	34.0	38.0	32.0	39.0
60-64	35.19416880109812	36.0	34.0	38.0	32.0	39.0
65-69	35.13524957548371	36.0	34.0	38.0	32.0	39.0
70-74	35.07312421550173	36.0	34.0	38.0	31.8	39.0
75-79	35.01013979530616	35.8	34.0	38.0	31.0	39.0
80-84	34.94484742051263	35.2	34.0	37.6	31.0	39.0
85-89	34.851678620302735	35.0	34.0	37.0	31.0	39.0
90-94	34.778185981693824	35.0	34.0	37.0	30.4	39.0
95-99	34.71578549141483	35.0	34.0	37.0	30.0	39.0
100-104	34.6585102552999	35.0	34.0	37.0	30.0	39.0
105-109	34.533147399995016	35.0	34.0	37.0	30.0	39.0
110-114	34.46484854835414	35.0	34.0	37.0	29.8	39.0
115-119	34.371969910100475	35.0	34.0	37.0	29.0	39.0
120-124	34.31205396790648	35.0	34.0	37.0	29.0	39.0
125-129	34.16652540661367	35.0	34.0	37.0	28.8	39.0
130-134	34.0761855595842	35.0	34.0	37.0	28.0	39.0
135-139	33.96296569165027	35.0	33.4	37.0	28.0	39.0
140-144	33.82504457981081	35.0	33.0	37.0	27.0	39.0
145-149	33.800675658001545	35.0	33.0	37.0	27.2	39.0
150	34.789696297376786	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	35.0
21	451.0
22	3555.0
23	17052.0
24	54389.0
25	120841.0
26	214151.0
27	333874.0
28	454443.0
29	560353.0
30	682833.0
31	850059.0
32	1094776.0
33	1493256.0
34	2229042.0
35	3914753.0
36	1.0669067E7
37	1484303.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.161593058661495	19.552195352000286	18.037365639641482	28.248845949696737
2	23.67765183424392	24.812177669433446	33.88091037863425	17.629260117688386
3	20.55485808969927	28.43856790914121	32.334807545403606	18.671766455755915
4	23.993873018038265	32.84703767751432	24.052953979843565	19.106135324603848
5	25.087759224211315	35.061434667815455	23.556676721446554	16.294129386526677
6	19.838332242982748	38.80356230916194	23.979111097011533	17.378994350843783
7	20.33311165100366	21.322141043483086	39.4178880392422	18.926859266271055
8	22.12978082402213	23.591225615133308	30.355873141676764	23.923120419167795
9	23.37798786701823	23.169353622222953	29.96092316413797	23.491735346620846
10-14	22.73208367589037	29.596786465154274	26.974271958845257	20.6968579001101
15-19	22.69576935563238	28.385644862095294	28.57317413291071	20.345411649361616
20-24	22.66666169229041	28.59159133974771	28.294066009763334	20.447680958198546
25-29	22.749321598854475	28.560196118693344	28.21247574702764	20.47800653542454
30-34	22.742083163424905	28.391715034127756	28.41826728022997	20.447934522217363
35-39	22.828078921666776	28.406506632126188	28.253137589449317	20.512276856757712
40-44	23.041965213645778	28.166630606580018	28.279745259580714	20.511658920193494
45-49	23.08154045215833	28.10159092194109	28.295278050566488	20.5215905753341
50-54	23.098566574111846	28.099372538769813	28.277810615825988	20.524250271292352
55-59	23.23252728098959	27.92205948679301	28.300647797712948	20.544765434504455
60-64	23.079507325986253	27.875843807159395	28.39613239167511	20.648516475179242
65-69	23.106854801267755	27.923922960733012	28.419129421324502	20.55009281667473
70-74	23.328134853938533	27.83880888232947	28.311536942274806	20.52151932145719
75-79	23.19614170944676	27.850256184232276	28.40751831110489	20.546083795216074
80-84	23.333316788679355	27.91700821036726	28.19682711589373	20.552847885059656
85-89	23.321128454265473	27.878962562199185	28.33389763640688	20.466011347128468
90-94	23.289064921663382	27.88531548202608	28.315136315696865	20.510483280613673
95-99	23.235578786240904	28.012398572168806	28.349486821201815	20.402535820388476
100-104	23.43738696920241	27.954283784911603	28.18283640099814	20.425492844887845
105-109	23.21955723243603	28.027400204027703	28.418533137574098	20.33450942596217
110-114	23.33744621758752	27.980376471881684	28.31335081314657	20.368826497384227
115-119	23.38531701049379	27.994797506131224	28.388865226028166	20.23102025734682
120-124	23.439040428591635	27.998361717039373	28.419688738649967	20.14290911571902
125-129	23.367952109690407	28.193687305033315	28.282310105750174	20.1560504795261
130-134	23.50825297747744	28.166310033664605	28.238595395401266	20.086841593456686
135-139	23.30969525888864	28.202965848600552	28.442428386287975	20.04491050622283
140-144	23.263195123684465	28.274180905405856	28.328375942732535	20.134248028177144
145-149	23.14398371504958	28.252280726050174	28.33031850328987	20.27341705561038
150	23.691597243488328	27.09721301872686	28.4230132613001	20.788176476484722
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	4.5
8	10.0
9	13.5
10	25.5
11	34.5
12	55.0
13	98.5
14	157.0
15	264.5
16	413.0
17	716.5
18	1253.5
19	1993.5
20	3144.5
21	5040.5
22	7579.0
23	11421.5
24	16917.5
25	25145.5
26	46401.0
27	64136.5
28	76606.0
29	104787.0
30	139681.5
31	174741.5
32	222541.0
33	287132.0
34	362192.5
35	456347.0
36	579300.5
37	720782.5
38	865643.0
39	1031797.5
40	1217518.5
41	1383462.5
42	1527346.0
43	1621953.5
44	1650136.0
45	1654187.0
46	1605289.0
47	1477462.5
48	1313344.5
49	1124573.0
50	932236.0
51	763223.0
52	613935.5
53	511089.5
54	425970.5
55	333470.5
56	248347.0
57	174491.5
58	119764.0
59	78051.0
60	53801.5
61	41339.0
62	30370.5
63	20533.5
64	14079.5
65	9593.0
66	6352.0
67	4474.5
68	3424.0
69	2545.0
70	1879.0
71	1501.5
72	1182.0
73	999.5
74	1022.5
75	936.5
76	655.0
77	323.0
78	141.0
79	64.0
80	19.0
81	6.0
82	3.0
83	2.5
84	3.0
85	1.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.272243162256865E-6
3	1.2408364743385296E-4
4	0.0010174859089575944
5	0.16734747917245638
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	1.406281337583667E-5
25-29	0.06564273116545691
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.272243162256864E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.274370376886897E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	1.0
62-63	5.0
64-65	1.0
66-67	3.0
68-69	10.0
70-71	17.0
72-73	23.0
74-75	30.0
76-77	31.0
78-79	47.0
80-81	72.0
82-83	91.0
84-85	115.0
86-87	132.0
88-89	167.0
90-91	190.0
92-93	190.0
94-95	270.0
96-97	315.0
98-99	319.0
100-101	378.0
102-103	397.0
104-105	424.0
106-107	470.0
108-109	498.0
110-111	552.0
112-113	542.0
114-115	601.0
116-117	621.0
118-119	719.0
120-121	771.0
122-123	884.0
124-125	1030.0
126-127	1226.0
128-129	1311.0
130-131	1543.0
132-133	1987.0
134-135	2462.0
136-137	3370.0
138-139	5795.0
140-141	9510.0
142-143	13957.0
144-145	65199.0
146-147	738716.0
148-149	1185427.0
150-151	2.2136819E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48900864738568
#Duplication Level	Percentage of total
1	28.594391919362643
2	10.611830578500985
3	6.88035261137651
4	4.62522618973991
5	3.637867093741224
6	2.766335537128301
7	2.3752044825862852
8	1.9747776227068323
9	1.6335741882742172
>10	19.321495078263485
>50	5.589535700673575
>100	8.478730368806382
>500	1.7136768334572874
>1k	1.5749340916255086
>5k	0.2220677037569754
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2408364743385298E-5	0.0	0.0	0.0	0.0015138204986930063	4.1361215811284326E-6
2	3.7225094230155895E-5	0.0	0.0	8.272243162256865E-6	0.009426221083391698	4.1361215811284326E-6
3	1.6130874166400886E-4	0.0	0.0	8.272243162256865E-6	0.011461192901306887	4.1361215811284326E-6
4	2.812562675167334E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.014207577631176166	8.272243162256865E-6
5	3.3916196965253143E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.017504066531335526	8.272243162256865E-6
6	3.598425775581736E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.019989875601593713	8.272243162256865E-6
7	4.0120379336945795E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.022368145510742562	8.272243162256865E-6
8	4.5497337392412756E-4	4.1361215811284326E-6	0.0	8.272243162256865E-6	0.027637564405100186	8.272243162256865E-6
9	7.445018846031178E-4	4.1361215811284326E-6	0.0	1.2408364743385298E-5	0.0382880774765059	8.272243162256865E-6
10-11	0.0011891349545744244	4.1361215811284326E-6	0.0	1.2408364743385298E-5	0.04740822556289409	8.272243162256865E-6
12-13	0.0012449725959196582	4.1361215811284326E-6	0.0	2.068060790564216E-5	0.05395570602582041	8.272243162256865E-6
14-15	0.0013152866627988416	4.1361215811284326E-6	0.0	2.8952851067899026E-5	0.07297359305584894	1.0340303952821082E-5
16-17	0.001350443696238433	4.1361215811284326E-6	0.0	3.308897264902746E-5	0.08960493793356636	1.2408364743385298E-5
18-19	0.0016689250579853226	4.1361215811284326E-6	0.0	4.136121581128432E-5	0.09573673817758926	1.4476425533949513E-5
20-21	0.0020308356963340602	4.1361215811284326E-6	0.0	4.963345897354119E-5	0.10280123383815662	1.654448632451373E-5
22-23	0.002409290821007312	4.1361215811284326E-6	0.0	4.963345897354119E-5	0.10967753596678265	2.0680607905642164E-5
24-25	0.002458924279980853	6.204182371692649E-6	0.0	4.963345897354119E-5	0.11439271456926906	2.4816729486770595E-5
26-27	0.002504421617373266	1.0340303952821082E-5	0.0	4.963345897354119E-5	0.11847713463063339	3.722509423015589E-5
28-29	0.002742248608288151	1.2408364743385298E-5	0.0	4.963345897354119E-5	0.12339705125138566	4.5497337392412756E-5
30-31	0.003073138334778425	1.2408364743385298E-5	0.0	5.3769580554669625E-5	0.1270533827291032	4.5497337392412756E-5
32-33	0.003106227307427453	1.2408364743385298E-5	0.0	6.204182371692648E-5	0.1307572796050037	4.5497337392412756E-5
34-35	0.0032509915627669476	1.2408364743385298E-5	0.0	6.204182371692648E-5	0.13406410880911587	4.963345897354119E-5
36-37	0.003279944413834847	1.2408364743385298E-5	0.0	6.617794529805492E-5	0.13626245742948564	5.3769580554669625E-5
38-39	0.0034309128515460346	1.2408364743385298E-5	0.0	6.617794529805492E-5	0.13820436651182544	6.20418237169265E-5
40-41	0.003511567222378039	1.2408364743385298E-5	0.0	7.238212766974756E-5	0.1402889717887142	6.617794529805492E-5
42-43	0.0037845512467325156	1.2408364743385298E-5	0.0	7.445018846031179E-5	0.14214609037864084	8.065437083200444E-5
44-45	0.00415473412824351	1.2408364743385298E-5	0.0	7.858631004144022E-5	0.1444478420385388	9.099467478482551E-5
46-47	0.0045518018000318405	1.4476425533949513E-5	0.0	7.858631004144022E-5	0.14642284009352763	1.1374334348103189E-4
48-49	0.004721382784858105	1.654448632451373E-5	0.0	8.272243162256866E-5	0.14830684347373163	1.2615170822441717E-4
50-51	0.005124654639018128	1.654448632451373E-5	0.0	8.685855320369709E-5	0.15025909286002426	1.4890037692062355E-4
52-53	0.0053997067241631684	1.654448632451373E-5	0.0	9.513079636595394E-5	0.15227131600924326	1.881935319413437E-4
54-55	0.005662350444564824	1.654448632451373E-5	0.0	9.926691794708238E-5	0.15379134069030792	2.2335056538093535E-4
56-57	0.005883632949155195	1.654448632451373E-5	0.0	9.926691794708238E-5	0.15534445434402167	2.77120145935605E-4
58-59	0.0059849679278928414	1.654448632451373E-5	0.0	9.926691794708238E-5	0.1566142436694281	3.1434524016576087E-4
60-61	0.006183501763787007	2.068060790564216E-5	0.0	9.926691794708238E-5	0.1579543470617137	3.453661520242241E-4
62-63	0.006837008973605299	2.068060790564216E-5	0.0	9.926691794708238E-5	0.15925722535976916	4.2395246206566433E-4
64-65	0.007600123405323495	2.4816729486770595E-5	0.0	1.013349787376466E-4	0.1602664390255645	4.7772204262033394E-4
66-67	0.008187452669843732	2.4816729486770595E-5	0.0	1.0340303952821081E-4	0.16133769451507676	5.066748936882329E-4
68-69	0.008758237448039455	2.4816729486770595E-5	0.0	1.0753916110933924E-4	0.1623572484848249	5.335596839655678E-4
70-71	0.009070514627414651	2.4816729486770595E-5	0.0	1.1787946506216032E-4	0.16336025796824855	6.224862979598291E-4
72-73	0.009955644645776137	2.4816729486770595E-5	0.0	1.24083647433853E-4	0.1642143670747516	6.907323040484482E-4
74-75	0.010408549958909701	2.4816729486770595E-5	0.0	1.2821976901498141E-4	0.16506640812046405	7.217532159069114E-4
76-77	0.010735303563818847	2.4816729486770595E-5	0.0	1.3028782980554563E-4	0.16611698300207067	7.527741277653748E-4
78-79	0.010933837399713011	2.4816729486770595E-5	0.0	1.3442395138667406E-4	0.16738884038826768	7.734547356710169E-4
80-81	0.011225433971182565	2.4816729486770595E-5	0.0	1.406281337583667E-4	0.16848284454647613	8.230881946445581E-4
82-83	0.011399151077589958	2.4816729486770595E-5	0.0	1.4476425533949512E-4	0.16953548748887332	8.768577751992277E-4
84-85	0.01166386285878218	2.6884790277334812E-5	0.0	1.4476425533949512E-4	0.1705529733978309	9.016745046859983E-4
86-87	0.011787946506216031	2.8952851067899026E-5	0.0	1.4683231613005937E-4	0.17153323421255834	9.161509302199479E-4
88-89	0.011808627114121674	2.8952851067899026E-5	0.0	1.4890037692062358E-4	0.17248867829779901	9.575121460312321E-4
90-91	0.011814831296493368	2.8952851067899026E-5	0.0	1.5924068087344465E-4	0.17364265621893385	9.988733618425164E-4
92-93	0.011837579965189573	2.8952851067899026E-5	0.0	1.6958098482626572E-4	0.17466634631026312	0.0010319623344915438
94-95	0.011845852208351831	3.102091185846325E-5	0.0	1.6958098482626572E-4	0.17562799457787548	0.0010629832463500072
96-97	0.011852056390723523	3.308897264902746E-5	0.0	1.7164904561682993E-4	0.1767654280126858	0.0010981402797895987
98-99	0.011868600877048037	3.7225094230155895E-5	4.1361215811284326E-6	1.799212887790868E-4	0.17808485079706576	0.001139501495600883
100-101	0.01187480505941973	3.7225094230155895E-5	4.1361215811284326E-6	1.8612547115077945E-4	0.17936911654800616	0.001184998832993296
102-103	0.01187480505941973	3.7225094230155895E-5	4.1361215811284326E-6	2.0060189668472898E-4	0.18033076481561852	0.0012304961703857088
104-105	0.01188514536337255	3.7225094230155895E-5	4.1361215811284326E-6	2.1301026142811426E-4	0.18137513551485346	0.0013235589059610985
106-107	0.01191409821444045	3.7225094230155895E-5	4.1361215811284326E-6	2.2335056538093535E-4	0.18228715032349227	0.0013814646080968964
108-109	0.011951323308670606	3.7225094230155895E-5	4.1361215811284326E-6	2.29554747752628E-4	0.18337495029932904	0.001435234188651566
110-111	0.011980276159738505	3.7225094230155895E-5	4.1361215811284326E-6	2.4403117328657752E-4	0.18474400654268258	0.0014786634652534146
112-113	0.012023705436340354	4.136121581128432E-5	4.1361215811284326E-6	2.4609923407714173E-4	0.18582560233614764	0.0015345011065986485
114-115	0.012040249922664868	4.5497337392412756E-5	4.1361215811284326E-6	2.647117811922197E-4	0.18673348102320533	0.0015655220184571117
116-117	0.012040249922664868	4.5497337392412756E-5	4.1361215811284326E-6	2.812562675167334E-4	0.18783782548536665	0.0016027471126872676
118-119	0.012052658287408253	4.5497337392412756E-5	4.1361215811284326E-6	2.812562675167334E-4	0.188718819382147	0.0016709931187758867
120-121	0.012052658287408253	5.1701519764105404E-5	4.1361215811284326E-6	2.812562675167334E-4	0.1897528497774291	0.001730966881702249
122-123	0.012060930530570509	5.376958055466962E-5	4.1361215811284326E-6	2.853923890978618E-4	0.1912377174250542	0.0018033490093719963
124-125	0.012069202773732766	5.376958055466962E-5	4.1361215811284326E-6	2.853923890978618E-4	0.1924165120756758	0.0018591866507172304
126-127	0.012073338895313895	5.376958055466962E-5	4.1361215811284326E-6	2.853923890978618E-4	0.1935270607202088	0.001931568778386978
128-129	0.012085747260057279	5.790570213579805E-5	4.1361215811284326E-6	2.936646322601187E-4	0.1947637610729662	0.0020039509060567253
130-131	0.012096087564010101	7.031406687918335E-5	4.1361215811284326E-6	2.936646322601187E-4	0.1960108017296764	0.0020639246689830876
132-133	0.012098155624800665	7.858631004144022E-5	6.204182371692649E-6	2.936646322601187E-4	0.1972888632982451	0.0021301026142811425
134-135	0.012102291746381793	8.065437083200443E-5	8.272243162256865E-6	2.936646322601187E-4	0.19826912411297254	0.0021735318908829913
136-137	0.012104359807172357	8.479049241313287E-5	8.272243162256865E-6	2.936646322601187E-4	0.19956786628944687	0.002208688924322583
138-139	0.012106427867962921	8.685855320369709E-5	8.272243162256865E-6	2.936646322601187E-4	0.20068461911635155	0.002247982079343303
>>END_MODULE
