##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24182374
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	66-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04406138950626	37.0	35.0	38.0	33.0	39.0
2	35.173329508508964	35.0	34.0	38.0	32.0	39.0
3	35.379366434412105	36.0	34.0	38.0	32.0	39.0
4	35.32665047691347	36.0	35.0	38.0	32.0	39.0
5	35.217385108674605	36.0	34.0	37.0	32.0	39.0
6	35.11941627401843	36.0	34.0	37.0	32.0	39.0
7	34.984973601020315	35.0	34.0	37.0	32.0	39.0
8	35.14726213398238	35.0	34.0	37.0	32.0	39.0
9	35.09527646045008	35.0	34.0	37.0	32.0	39.0
10-14	35.12340450114617	35.4	34.0	37.4	32.0	39.0
15-19	35.08254278095277	36.0	34.0	37.0	32.0	39.0
20-24	35.07616119906176	36.0	34.0	37.0	32.0	39.0
25-29	35.05113559983813	36.0	34.0	37.0	32.0	39.0
30-34	35.00979677181405	36.0	34.0	37.0	31.2	39.0
35-39	34.963783448225556	35.6	34.0	37.0	31.0	39.0
40-44	34.93034045375363	35.2	34.0	37.0	31.0	39.0
45-49	34.907046917726106	35.0	34.0	37.0	31.0	39.0
50-54	34.86466351070412	35.0	34.0	37.0	31.0	39.0
55-59	34.82837641994951	35.0	34.0	37.0	31.0	39.0
60-64	34.79252969125364	35.0	34.0	37.0	31.0	39.0
65-69	34.743025363821594	35.0	34.0	37.0	30.6	39.0
70-74	34.717275280844824	35.0	34.0	37.0	30.0	39.0
75-79	34.672453584689	35.0	34.0	37.0	30.0	39.0
80-84	34.6332411860138	35.0	34.0	37.0	30.0	39.0
85-89	34.58943399953689	35.0	34.0	37.0	30.0	39.0
90-94	34.540315421113874	35.0	34.0	37.0	30.0	39.0
95-99	34.48590885565962	35.0	34.0	37.0	30.0	39.0
100-104	34.43672764963513	35.0	34.0	37.0	30.0	39.0
105-109	34.39816458821031	35.0	34.0	37.0	30.0	39.0
110-114	34.3324680132344	35.0	34.0	37.0	29.0	39.0
115-119	34.29164901988157	35.0	34.0	37.0	29.0	39.0
120-124	34.234096040467975	35.0	34.0	37.0	29.0	39.0
125-129	34.168786958962656	35.0	34.0	37.0	29.0	39.0
130-134	34.11020406629692	35.0	34.0	37.0	28.4	39.0
135-139	34.04704053093552	35.0	34.0	37.0	28.0	39.0
140-144	33.97720144716709	35.0	34.0	37.0	28.0	39.0
145-149	33.98142438280171	35.0	33.4	37.0	28.0	39.0
150	34.8676626104615	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	6.0
22	83.0
23	1206.0
24	10138.0
25	48824.0
26	146649.0
27	309394.0
28	491339.0
29	664118.0
30	851261.0
31	1099594.0
32	1452788.0
33	1987930.0
34	2876123.0
35	4640200.0
36	8384222.0
37	1218451.0
38	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.09481021176829	11.90516282644541	14.645431420422165	37.35459554136414
2	21.74779452986588	13.334941123573277	34.88535128169787	30.031913064862962
3	20.86377705508794	18.074128855745855	27.42193851846911	33.6401555706971
4	24.18526677255718	25.779703343904465	22.079599011008504	27.955430872529845
5	23.84582731910429	32.16856092912835	24.23183795873293	19.753773793034433
6	18.349762683186675	38.15202989335434	25.60347306251437	17.89473436094461
7	14.811232291112542	26.15151602368154	40.397233370914684	18.640018314291236
8	17.37896344417359	26.214356093623998	31.901691900151924	24.50498856205049
9	18.119768775341992	24.79883960910746	32.876315285840874	24.205076329709676
10-14	19.12760311701687	30.853156676253317	27.388498583456478	22.630741623273337
15-19	19.204486131297312	29.27826354181177	28.46104017439123	23.05621015249969
20-24	19.31120095927634	29.405799849185115	28.09629193484432	23.186707256694223
25-29	19.435878894184857	29.494028017610933	27.938945649314373	23.131147438889833
30-34	19.36141545046803	29.388440683910783	27.90151443398526	23.348629431635935
35-39	19.436085866751327	29.209769961533894	28.082874444240723	23.271269727474056
40-44	19.43177573723203	29.295060885028608	28.008021261009546	23.265142116729816
45-49	19.53273045885596	29.124541754156873	27.997188624359413	23.34553916262775
50-54	19.53452405396647	29.19182185642623	27.998471478298313	23.27518261130899
55-59	19.630325421169108	29.12177970172536	27.913314875273286	23.334580001832247
60-64	19.65361005042616	29.040247395848517	27.871010178680137	23.435132375045185
65-69	19.61173210483544	28.928950125914643	28.03744892418202	23.421868845067902
70-74	19.707899356348744	28.921780554826142	28.000184432034107	23.370135656791007
75-79	19.601738804878813	28.92136842439392	27.9970332694591	23.479859501268173
80-84	19.791631017333778	28.781743675373455	27.901655826705614	23.52496948058716
85-89	19.772052632903105	28.820472237997375	28.01027866931062	23.397196459788905
90-94	19.915096921134456	28.689544555466256	27.94000051641472	23.455358006984568
95-99	19.96101268950553	28.692100429381135	27.963615659061375	23.38327122205196
100-104	19.98019376111498	28.6032529080473	27.95561203799899	23.460941292838733
105-109	19.93106307247423	28.580976188989016	28.010558914029883	23.47740182450687
110-114	20.006341512830613	28.605573352094964	27.93526224544209	23.452822889632337
115-119	20.073367602603163	28.540019248373504	27.9891067429827	23.397506406040634
120-124	20.09789067716403	28.389761222429993	28.07402636100403	23.43832173940195
125-129	20.16501930304181	28.39613781012678	28.013153146023267	23.425689740808142
130-134	20.09613461579216	28.520269096456076	27.975850987424682	23.40774530032708
135-139	20.154896929671377	28.370903882724864	27.985366914301586	23.488832273302172
140-144	20.25822523826212	28.28764589821488	28.062761562523757	23.391367300999242
145-149	19.932108435458215	28.233136125943215	28.313997582868417	23.52075785573015
150	20.19023971006915	27.16176239434884	28.523226291348834	24.12477160423317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.0
8	8.0
9	14.5
10	22.0
11	39.0
12	70.5
13	140.5
14	254.0
15	423.5
16	831.0
17	1607.0
18	3774.0
19	7004.5
20	7974.5
21	9490.5
22	14309.5
23	21002.0
24	30626.0
25	43246.0
26	58040.5
27	78061.5
28	107840.0
29	147426.5
30	194560.5
31	249222.0
32	305039.0
33	364169.0
34	434755.5
35	521055.0
36	620556.5
37	732550.0
38	855934.0
39	992345.5
40	1134360.5
41	1272198.0
42	1402314.0
43	1508410.0
44	1581940.0
45	1605255.5
46	1555390.5
47	1459756.0
48	1319319.0
49	1141549.5
50	960422.0
51	787475.0
52	632710.5
53	505167.0
54	407967.5
55	318707.0
56	238782.0
57	169583.0
58	112897.5
59	74628.5
60	52806.0
61	40644.0
62	30547.5
63	20902.0
64	13348.0
65	8865.0
66	5831.5
67	3944.5
68	2575.0
69	1705.5
70	1691.0
71	2166.5
72	2398.0
73	1748.5
74	990.0
75	578.0
76	278.5
77	100.5
78	30.0
79	14.0
80	6.5
81	3.5
82	3.5
83	3.0
84	3.5
85	3.5
86	1.0
87	1.0
88	1.0
89	0.0
90	1.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.7555297920708696E-4
3	7.65020010028792E-4
4	6.781799007822805E-4
5	5.747988183459573E-4
6	5.91339791535769E-4
7	6.864503873771864E-4
8	6.409627111052041E-4
9	7.360733069466215E-4
10-14	8.328380001070201E-4
15-19	6.128430566825242E-4
20-24	6.004373267901655E-4
25-29	7.005102145885263E-4
30-34	3.5232272894298965E-4
35-39	3.9946450253395307E-4
40-44	1.720261211740419E-4
45-49	2.0179987291570297E-4
50-54	2.2578428404092997E-4
55-59	2.3405477063583583E-4
60-64	1.827777537474195E-4
65-69	1.6210154128213074E-4
70-74	8.353192773469326E-5
75-79	2.0014589808921537E-4
80-84	5.210416940127404E-5
85-89	2.1503376832404054E-4
90-94	7.360806548550644E-5
95-99	7.60898491766032E-5
100-104	7.112819481694966E-5
105-109	1.2654364115928697E-4
110-114	5.8723803549026824E-5
115-119	1.4391719461186926E-4
120-124	1.679068364864831E-4
125-129	8.271496453000348E-5
130-134	8.768108252851198E-5
135-139	1.5303921022568627E-4
140-144	0.033802632659817526
145-149	9.241396837131933E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	1.0
70-71	1.0
72-73	4.0
74-75	4.0
76-77	8.0
78-79	7.0
80-81	20.0
82-83	31.0
84-85	25.0
86-87	44.0
88-89	41.0
90-91	46.0
92-93	72.0
94-95	86.0
96-97	92.0
98-99	91.0
100-101	108.0
102-103	118.0
104-105	129.0
106-107	130.0
108-109	158.0
110-111	159.0
112-113	161.0
114-115	169.0
116-117	195.0
118-119	178.0
120-121	210.0
122-123	245.0
124-125	260.0
126-127	314.0
128-129	300.0
130-131	403.0
132-133	509.0
134-135	655.0
136-137	1002.0
138-139	2147.0
140-141	4454.0
142-143	7434.0
144-145	52933.0
146-147	624681.0
148-149	1230614.0
150-151	2.2254134E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.428855755246296
#Duplication Level	Percentage of total
1	36.87471162420175
2	10.89229489927526
3	6.4483667729883525
4	4.473183863100121
5	3.4129935959558155
6	2.5666022183872688
7	2.0795254932184624
8	1.769332679479842
9	1.5455513392891347
>10	17.170018178775923
>50	4.683906138186324
>100	6.239869653060916
>500	1.114577281458549
>1k	0.7290662626223768
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.13524329745293E-6	1.5713924530321135E-4	1.364630288159467E-4
2	0.0	0.0	0.0	8.27048659490586E-6	0.0018071013209869304	3.1841373390387563E-4
3	8.27048659490586E-6	0.0	0.0	8.27048659490586E-6	0.003117973446279509	3.9284811325802834E-4
4	1.654097318981172E-5	0.0	0.0	1.240572989235879E-5	0.004635607736444734	4.7141773590963403E-4
5	2.067621648726465E-5	0.0	0.0	1.240572989235879E-5	0.006273164082236095	5.334463853714279E-4
6	2.481145978471758E-5	0.0	0.0	1.240572989235879E-5	0.007493060854984709	5.747988183459573E-4
7	2.481145978471758E-5	0.0	0.0	1.240572989235879E-5	0.00856822411232247	6.285569812128454E-4
8	2.481145978471758E-5	0.0	0.0	1.654097318981172E-5	0.010482841759043177	6.823151440797335E-4
9	2.481145978471758E-5	0.0	0.0	1.654097318981172E-5	0.01417147878037119	7.526142801364332E-4
10-11	2.894670308217051E-5	0.0	0.0	1.654097318981172E-5	0.018941481923983147	8.725363357625683E-4
12-13	2.894670308217051E-5	0.0	0.0	2.067621648726465E-5	0.022566022674200638	9.697145532527121E-4
14-15	2.894670308217051E-5	0.0	0.0	2.481145978471758E-5	0.02682945851387461	0.0011123804470148382
16-17	3.514956802834991E-5	0.0	0.0	3.7217189677076366E-5	0.030691775753695645	0.001209558664504982
18-19	4.548767627198223E-5	0.0	0.0	4.13524329745293E-5	0.03381181682162388	0.0013005340170489463
20-21	4.962291956943516E-5	0.0	0.0	4.755529792070869E-5	0.03722132492037382	0.0013853065046467315
22-23	5.789340616434102E-5	0.0	0.0	4.962291956943516E-5	0.04048403188206419	0.0014824847221368755
24-25	5.789340616434102E-5	0.0	0.0	4.962291956943516E-5	0.04353584143558445	0.0015900010478706516
26-27	5.789340616434102E-5	0.0	0.0	5.1690541218161624E-5	0.04720793748372265	0.0016520296973324454
28-29	5.789340616434102E-5	0.0	0.0	5.789340616434102E-5	0.050995820344189535	0.0017326669416327777
30-31	6.202864946179395E-5	0.0	0.0	6.616389275924688E-5	0.05491809861182363	0.0018174394292305627
32-33	6.616389275924688E-5	0.0	0.0	7.856962265160566E-5	0.05828832189924778	0.0019373614848566976
34-35	6.616389275924688E-5	0.0	0.0	8.27048659490586E-5	0.0615014059413687	0.002059351162131559
36-37	6.616389275924688E-5	4.13524329745293E-6	0.0	8.684010924651152E-5	0.06482614155252087	0.002133785541485712
38-39	7.029913605669981E-5	4.13524329745293E-6	0.0	9.097535254396446E-5	0.06792550640396183	0.002189611326001327
40-41	7.029913605669981E-5	6.202864946179395E-6	0.0	9.097535254396446E-5	0.07118821336565219	0.0023012628950325555
42-43	7.029913605669981E-5	8.27048659490586E-6	4.13524329745293E-6	9.097535254396446E-5	0.07427930773049826	0.002435658302199776
44-45	7.029913605669981E-5	8.27048659490586E-6	4.13524329745293E-6	9.304297419269092E-5	0.07732077917577489	0.0025287012763924666
46-47	7.443437935415274E-5	8.27048659490586E-6	4.13524329745293E-6	9.924583913887032E-5	0.08049251078492128	0.002611406142341525
48-49	7.443437935415274E-5	8.27048659490586E-6	6.202864946179395E-6	9.924583913887032E-5	0.08475181138129781	0.0026775700351007723
50-51	7.856962265160566E-5	8.27048659490586E-6	8.27048659490586E-6	1.0751632573377618E-4	0.08799177450485217	0.0027768158742396427
52-53	8.27048659490586E-5	8.27048659490586E-6	8.27048659490586E-6	1.1165156903122911E-4	0.09104565168002116	0.0028450473886476157
54-55	8.27048659490586E-5	8.27048659490586E-6	8.27048659490586E-6	1.1785443397740851E-4	0.09414088128816467	0.0029008731731632303
56-57	8.477248759778506E-5	1.240572989235879E-5	8.27048659490586E-6	1.2819254222104083E-4	0.0971968260849824	0.0029525637143813918
58-59	8.684010924651152E-5	1.240572989235879E-5	8.27048659490586E-6	1.2819254222104083E-4	0.09981650271391883	0.003033200958681724
60-61	8.684010924651152E-5	1.240572989235879E-5	8.27048659490586E-6	1.2819254222104083E-4	0.10254369566858904	0.0031138382029820564
62-63	9.097535254396446E-5	1.240572989235879E-5	8.27048659490586E-6	1.2819254222104083E-4	0.10522540094698726	0.0032316926369594648
64-65	9.097535254396446E-5	1.240572989235879E-5	8.27048659490586E-6	1.3232778551849377E-4	0.10822551995928936	0.003279247934880173
66-67	9.097535254396446E-5	1.240572989235879E-5	8.27048659490586E-6	1.3232778551849377E-4	0.11112019026750641	0.0033640204224779586
68-69	9.717821749014385E-5	1.240572989235879E-5	8.27048659490586E-6	1.3232778551849377E-4	0.1137522726263352	0.003432251936885932
70-71	1.0131346078759678E-4	1.240572989235879E-5	8.27048659490586E-6	1.3232778551849377E-4	0.11641536930989488	0.003481874856455367
72-73	1.0751632573377618E-4	1.240572989235879E-5	8.27048659490586E-6	1.364630288159467E-4	0.1189482058295848	0.0035790530739455107
74-75	1.1165156903122911E-4	2.2743838135991114E-5	8.27048659490586E-6	1.364630288159467E-4	0.12166919591930883	0.003696907507922919
76-77	1.1578681232868204E-4	2.481145978471758E-5	8.27048659490586E-6	1.364630288159467E-4	0.1246672473099622	0.0037816799955207045
78-79	1.2198967727486144E-4	2.481145978471758E-5	8.27048659490586E-6	1.364630288159467E-4	0.12765702821402067	0.003827167671792687
80-81	1.240572989235879E-4	2.481145978471758E-5	8.27048659490586E-6	1.364630288159467E-4	0.13057030711707626	0.0038829934563083014
82-83	1.2612492057231437E-4	2.481145978471758E-5	8.27048659490586E-6	1.364630288159467E-4	0.1335435470479449	0.003932616375877737
84-85	1.3439540716722023E-4	2.6879081433444045E-5	8.27048659490586E-6	1.364630288159467E-4	0.13633483627372567	0.003994645025339531
86-87	1.364630288159467E-4	2.894670308217051E-5	8.27048659490586E-6	1.364630288159467E-4	0.13947348593649242	0.004081485134586041
88-89	1.4473351541085256E-4	2.894670308217051E-5	8.27048659490586E-6	1.364630288159467E-4	0.14229372186535533	0.004168325243832554
90-91	1.5300400200575842E-4	2.894670308217051E-5	8.27048659490586E-6	1.364630288159467E-4	0.14505399676640515	0.004211745298455809
92-93	1.6127448860066426E-4	2.894670308217051E-5	8.27048659490586E-6	1.364630288159467E-4	0.14737180063462751	0.004308923515945954
94-95	1.6334211024939072E-4	2.894670308217051E-5	8.27048659490586E-6	1.364630288159467E-4	0.1494642337431387	0.004379222652002654
96-97	1.7574784014174951E-4	3.1014324730896976E-5	8.27048659490586E-6	1.364630288159467E-4	0.15154632874340626	0.00443918367981572
98-99	1.7781546179047598E-4	3.308194637962344E-5	8.27048659490586E-6	1.364630288159467E-4	0.15338237676747535	0.004488806599385156
100-101	1.7988308343920245E-4	3.308194637962344E-5	8.27048659490586E-6	1.364630288159467E-4	0.15555131187698942	0.004548767627198223
102-103	1.8608594838538184E-4	3.308194637962344E-5	8.27048659490586E-6	1.364630288159467E-4	0.15763340687725697	0.004612863898308743
104-105	1.8608594838538184E-4	4.13524329745293E-5	8.27048659490586E-6	1.364630288159467E-4	0.15933505949415885	0.004672824926121811
106-107	1.8608594838538184E-4	4.13524329745293E-5	8.27048659490586E-6	1.4473351541085256E-4	0.16116283703163303	0.004734853575583605
108-109	1.8608594838538184E-4	4.13524329745293E-5	8.27048659490586E-6	1.6334211024939075E-4	0.16345376181842197	0.00477827363020686
110-111	1.8608594838538184E-4	4.13524329745293E-5	8.27048659490586E-6	1.7781546179047598E-4	0.16575709233510325	0.004850640387912287
112-113	1.8608594838538184E-4	4.13524329745293E-5	8.27048659490586E-6	1.7988308343920245E-4	0.16754558506125164	0.004920939523968987
114-115	1.881535700341083E-4	4.13524329745293E-5	8.27048659490586E-6	1.8401832673665538E-4	0.16917487092044808	0.00498917103837696
116-117	2.0262692157519357E-4	4.13524329745293E-5	8.27048659490586E-6	1.8608594838538184E-4	0.17081863013118564	0.005022252984756583
118-119	2.0469454322392003E-4	4.548767627198223E-5	8.27048659490586E-6	1.943564349802877E-4	0.17272497729131142	0.005057402552784933
120-121	2.067621648726465E-4	4.548767627198223E-5	8.27048659490586E-6	2.0262692157519357E-4	0.17449486142262127	0.00509461974246201
122-123	2.067621648726465E-4	4.548767627198223E-5	8.27048659490586E-6	2.067621648726465E-4	0.17664725555894553	0.005166986500167435
124-125	2.067621648726465E-4	4.548767627198223E-5	8.27048659490586E-6	2.0882978652137296E-4	0.17831169098617033	0.0052083389331419655
126-127	2.067621648726465E-4	4.548767627198223E-5	8.27048659490586E-6	2.1503265146755236E-4	0.18011672468550854	0.005241420879521589
128-129	2.1089740817009943E-4	4.548767627198223E-5	8.27048659490586E-6	2.2123551641373176E-4	0.1821202500631245	0.005274502825901212
130-131	2.1710027311627882E-4	4.548767627198223E-5	1.654097318981172E-5	2.2743838135991115E-4	0.1840203943583041	0.005297246664037204
132-133	2.2330313806245822E-4	4.548767627198223E-5	2.067621648726465E-5	2.3157362465736408E-4	0.1855359610268206	0.005332396232065553
134-135	2.2743838135991115E-4	4.755529792070869E-5	2.067621648726465E-5	2.3157362465736408E-4	0.18713216493963744	0.005377883908337535
136-137	2.2743838135991115E-4	4.962291956943516E-5	2.067621648726465E-5	2.3157362465736408E-4	0.18935072296872094	0.005408898233068433
138-139	2.2743838135991115E-4	4.962291956943516E-5	2.067621648726465E-5	2.3984411125226995E-4	0.19093038590834796	0.005444047801096782
>>END_MODULE
